NAVER Academic | Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk...
To test this hypothesis, SF scaffolds with different pore diameters were prepared and seeded with human mesenchymal stem cells ... of the newly formed tissue and suggested that the structure of tissue-engineered bone was controlled by the underlying scaffold ... dynamic cell seeding in spinner flasks resulted in equal cell viability and proliferation, and better cell distribution ... while keeping scaffold porosities constant among groups. We hypothesized that tissue engineered bone-like structure resulting ...http://academic.naver.com/article.naver?doc_id=64838896
Mesenchymal stem cell cultivation in electrospun scaffolds: mechanistic modeling for tissue engineering | Springer for Research...
... and processes can be optimized to develop a tissue substitute. Three-dimensional (3D) architectural... ... Tissue engineering is a multidisciplinary field of research in which the cells, biomaterials, ... The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo. J. Biomed ... focusing on mesenchymal stem cell culture in electrospun scaffolds.. Keywords. Stem cells Tissue development Electrospun ...https://rd.springer.com/article/10.1007%2Fs10867-018-9482-y
A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled...
... proliferation and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) to chondrocytes in culture with ... The scaffold was fabricated by simple freeze-drying and cross-linking techniques. On histology, scaffolds showed most of the ... Human cartilage was physically shattered, then decellularized sequentially with use of hypotonic buffer, TritonX-100, and a ... and PKH26 fluorescent labeling and in vivo fluorescent imaging can be useful for cell tracking and analyzing cell-scaffold ...https://www.semanticscholar.org/paper/A-cartilage-ECM-derived-3-D-porous-acellular-matrix-Yang-Peng/475874697bc633d7e48f2c588c8682b4fdafb573
Bone Regeneration using Silk Hydroxyapatite Hybrid Composite in a Rat Alveolar Defect Model
Engineering cartilage-like tissue using human mesenchymal stem cells and silk protein scaffolds. Biotechnol Bioeng. 2004;88:379 ... Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold. J Cranio- ... Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. J Biomed Mater Res. 2004;71A:25- ... In vitro cartilage tissue engineering with 3D porous aqueous-derived silk scaffolds and mesenchymal stem cells. Biomaterials. ...http://www.medsci.org/v15p0059.htm
Coupling of gelatin to inner surfaces of pore walls in spongy alginate-based scaffolds facilitates the adhesion, growth and...
We have developed a novel wide-pore scaffold for cell 3D culturing, based on the technology of freeze-drying of Ca-alginate and ... Phenotype and gene expression of human mesenchymal stem cells in alginate scaffolds. Tissue Eng Part A. 2009;15:1763-73. ... Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials. 2005;26 ... Assessment of stem cell/biomaterial combinations for stem cell-based tissue engineering. Biomaterials. 2008;29:302-13.CrossRef ...https://link.springer.com/article/10.1007%2Fs10856-011-4323-6
Janne Elin Reseland - Institutt for klinisk odontologi
Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells ... In addition, secretion of vascular endothelial growth factor A was significantly enhanced from cells cultured on scaffolds with ... Ameloblastin peptides modulates the osteogenic capacity of human mesenchymal stem cells. Frontiers in Physiology. ISSN 1664- ... Gordeladze, Jan Oxholm & Reseland, Janne Elin (2014). Stem Cells and Scaffold Materials for Osteoblast Engineering: Guidance ...http://www.odont.uio.no/iko/personer/vit/jreselan/
Håvard Jostein Haugen - Institutt for klinisk odontologi
Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells ... In addition, secretion of vascular endothelial growth factor A was significantly enhanced from cells cultured on scaffolds with ... The role of new zinc incorporated monetite cements on osteogenic differentiation of human mesenchymal stem cells. Materials ... zinc incorporated bone cements with osteogenic differentiation potential towards human mesenchymal stem cells (hMSCs). XRD ...http://www.odont.uio.no/iko/personer/vit/haavarjh/
Preparation and investigation of polylactic acid, calcium carbonate and polyvinylalcohol nanofibrous scaffolds for osteogenic...
MTT and ALP results showed that alignment of nanofiber increased the osteogenic differentiation of stem cells.Conclusion: ... The surface morphology of prepared nanofibrous scaffolds with and without cell was examined using scanning electron microscopy ... The in vitro properties of fabricated scaffolds was also investigated by the MTT assay and alkaline phosphatase activity (ALP). ... Materials and Methods: Aligned and random nanocomposite nanofibrous scaffolds were electrospun from polylactic acid (PLA), poly ...http://nmj.mums.ac.ir/article_6508.html
Protocols and Video Articles Authored by Zhixiang Tong (Translated to Turkish)
... were able to maintain their mechanical integrity as well as the viability of encapsulated primary human mesenchymal stem cells ... Successful engineering of vocal fold tissues requires a strategic combination of therapeutic cells, biomimetic scaffolds, and ... Overall, the high-frequency vibratory stimulation, when combined with a synthetic fibrous scaffold, serves as a potent ... Dynamic Vibration Cooperates with Connective Tissue Growth Factor to Modulate Stem Cell Behaviors Tissue Engineering. Part A. ...https://www.jove.com/author/Zhixiang_Tong?language=Turkish
Protein-Based Hydrogels | SpringerLink
Novel soy protein scaffolds for tissue regeneration: material characterization and interaction with human mesenchymal stem ... Combination of collagen-based scaffold and bioactive factors induces adipose-derived mesenchymal stem cells chondrogenic ... Collagen-hydroxyapatite scaffolds induce human adipose derived stem cells osteogenic differentiation in vitro. PLoS One 11(3): ... Transforming growth factor beta-releasing scaffolds for cartilage tissue engineering. Tissue Eng Part B Rev 20(2):106-125PubMed ...https://link.springer.com/referenceworkentry/10.1007%2F978-3-319-76573-0_52-1
Stem and progenitor cells: advancing bone tissue engineering | SpringerLink
Unlike many other postnatal tissues, bone can regenerate and repair itself; nevertheless, this capacity can be overcome. ... Bone regeneration of mouse critical-sized calvarial defects with human mesenchymal stem cells in scaffold. Lab Anim Res. 2013; ... reconstruction of rat calvarial defects achieved by plasma-treated electrospun scaffolds and induced pluripotent stem cells. ... Functional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice. ...https://link.springer.com/article/10.1007%2Fs13346-015-0235-1
Protocols and Video Articles Authored by Zhixiang Tong
... were able to maintain their mechanical integrity as well as the viability of encapsulated primary human mesenchymal stem cells ... Successful engineering of vocal fold tissues requires a strategic combination of therapeutic cells, biomimetic scaffolds, and ... This dynamic culture device, when combined with a fibrous poly(ε-caprolactone) scaffold, creates a vocal fold-mimetic ... Dynamic Vibration Cooperates with Connective Tissue Growth Factor to Modulate Stem Cell Behaviors Tissue Engineering. Part A. ...https://www.jove.com/author/Zhixiang_Tong
In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells | PNAS
2003) Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds. Proc Natl Acad Sci USA 100:12741- ... Histological examination of the implants revealed formation of connective tissue in the scaffold with increasing bone area (Fig ... 2006) Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells 24:1914-1922. ... In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells. Nathaniel S. ...https://www.pnas.org/content/105/52/20641
Enhanced osteogenesis in co-cultures with human mesenchymal stem cells and endothelial cells on polymeric microfiber scaffolds
bone tissue engineering; co-culture; mesenchymal stem cells; human umbilical vein endothelial cells; 3D culture; More... ... The results demonstrate that the combination of MSC and HUVEC populations within a porous scaffold material under osteogenic ... " human mesenchymal stem cells (hMSCs) and their osteogenically precultured derivatives were directly co-cultured with human ... Specifically, cells were cultured on scaffolds for up to three weeks, and the cellularity, alkaline phosphatase activity (ALP ...https://scholarship.rice.edu/handle/1911/71555
Patent US7022522 - Macroporous polymer scaffold containing calcium phosphate particles - Google Patents
The polymer scaffold has utility in the area of tissue engineering, particularly as a scaffold for both in vitro and in vivo ... cell growth. The polymer scaffold may be produced using pure polymer or alternatively a composite material may be formed ... The polymer scaffold embodies macropores having a diameter in a range of 0.5-3.5 mm, and preferably in a range of about 1.0-2.0 ... The polymer scaffold is prepared using a novel process which advantageously combines the techniques of particulate leaching and ...http://www.google.co.uk/patents/US7022522
Frontiers | Distribution and Viability of Fetal and Adult Human Bone Marrow Stromal Cells in a Biaxial Rotating Vessel...
Bone marrow derived mesenchymal stromal cells (MSCs) are promising candidates for tissue engineering based cell therapies due ... One of the hurdles to overcome when combining additive manufactured scaffolds with MSCs is the resulting heterogeneous cell ... Bone marrow derived mesenchymal stromal cells (MSCs) are promising candidates for tissue engineering based cell therapies due ... One of the hurdles to overcome when combining additive manufactured scaffolds with MSCs is the resulting heterogeneous cell ...https://www.frontiersin.org/articles/10.3389/fbioe.2015.00169/full
Fabrication of rnacroporous cement scaffolds using PEG particles : In vitro evaluation with induced pluripotent stem cell...
Bioinaterial scaffold, Calcium phosphate cement, Macroporosity, Mesenchymal progenitors, Induced pluripotent stem cells, Tissue ... then tested in vitro with human mesenchymal progenitors derived from induced pluripotent stem cells (iPSC-MPs). Biomimetic ... In vitro evaluation with induced pluripotent stem cell-derived mesenchymal progenitors. Sladkova, Martina New York Stem Cell ... CPCs are, consequently, very effective substrates (scaffolds) for tissue engineering; bone and stem cells attach readily, and ...http://uu.diva-portal.org/smash/record.jsf?pid=diva2:1040414
Effects of Matrix Stiffness on the Morphology, Adhesion, Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells
Close dependence of fibroblast proliferation on collagen scaffold matrix stiffness. J Tissue Eng Regen M. 2009;3:77-84 ... Mesenchymal stem cell fate is regulated by the composition and mechanical properties of collagen-glycosaminoglycan scaffolds. J ... stiffness regulation of integrin-mediated mechanotransduction during osteogenic differentiation of human mesenchymal stem cells ... Adult mesenchymal stem cells and their potency in the cell-based therapy. Journal of stem cells. 2013;8:1-16 ...http://www.medsci.org/v15p0257.htm
Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System | Protocol
This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell ... Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets…. Published 10/03/2014 ... Cui, X. J., et al. Transplantation of Mesenchymal Stem Cells with Self-Assembling Polypeptide Scaffolds Is Conducive to ... Cerqueira, M. T., et al. Human Adipose Stem Cells Cell Sheet Constructs Impact Epidermal Morphogenesis in Full-Thickness ...https://www.jove.com/video/58624/construction-multilayered-mesenchymal-stem-cell-sheet-with-3d-dynamic
Prospect of Stem Cells in Bone Tissue Engineering: A Review
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... accelerates bone healing mediated by human mesenchymal stem cell-embedded hydroxyapatite/tricalcium phosphate scaffold," PLoS ... J. D. Kretlow and A. G. Mikos, "Review: mineralization of synthetic polymer scaffolds for bone tissue engineering," Tissue ...https://www.hindawi.com/journals/sci/2016/6180487/ref/
IJMS | Free Full-Text | Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells...
Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue ... Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes ... Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we ... without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane ...http://www.mdpi.com/1422-0067/12/10/6749/htm
Brian Snyder, MD, PhD | Boston Children's Hospital
Bone tissue engineering using human mesenchymal stem cells: effects of scaffold material and medium flow. Ann Biomed Eng. 2004 ... Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. J Biomed Mater Res A. 2004 Oct 01 ... A Tissue-Penetrating Double Network Restores the Mechanical Properties of Degenerated Articular Cartilage. Angew Chem Int Ed ... Porous silk fibroin 3-D scaffolds for delivery of bone morphogenetic protein-2 in vitro and in vivo. J Biomed Mater Res A. 2006 ...https://www.childrenshospital.org/directory/physicians/s/brian-snyder
NAVER Academic | Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte...
Here, we focused on integrin-mediated cell-scaffold adhesion and prepared cell adhesive fibroin in which a tandem repeat of the ... Although both the sequence and fibronectin increased cell adhesive force, chondrocytes grown on the fibroin substrate exhibited ... specific cell adhesion to the RGDS predominately changed the chondrocyte adhesive state. This suggestion was also supported by ... Initial chondrocyte-silk fibroin interactions are implicated in chondrogenesis when using fibroin as a scaffold for ...http://academic.naver.com/article.naver?doc_id=64841569
Fibrin scaffold - Wikipedia
Each combined with bone marrow mesenchymal stem cells and bone morphogenetic protein 2 (BMP-2) are injected into the ... For some tissues like cartilage, highly dense polymeric scaffolds such as polyethylene glycol (PEG) are essential due to ... Firstly there are only few autologous vessels available in a human body that might be of low quality, considering the health ... From autologous arterial-derived cells and fibrin scaffold, tissue engineered heart valves are formed, then mechanically ...https://en.wikipedia.org/wiki/Fibrin_scaffold
Stem-cell therapy - Wikipedia
Fetal tissue implant Human Stem Cells Institute Induced pluripotent stem cell Induced stem cells Ian Murnaghan for Explore Stem ... Mesenchymal stem cells that are induced to a neural cell fate are loaded onto a porous scaffold and are then implanted at the ... Scaffolds composed of natural and artificial components are seeded with mesenchymal stem cells and placed in the defect. Within ... Stem cells can also be used to stimulate the growth of human tissues. In an adult, wounded tissue is most often replaced by ...https://en.wikipedia.org/wiki/Stem-cell_therapy