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*  KAKEN - Research Projects | Study on initiation of DNA repication in cell-free system of Xenopus eggs (KAKENHI-PROJECT-07680762)
... has an activity to untwist DNA and open double strand DNA to single strand DNA from cell-free DNA synthetic system of Xenopus ... Xenopus laevis / DNA rreplication / cell-free DNA replication / initiator protein / license factor / initation of DNA ... Study on initiation of DNA repication in cell-free system of Xenopus eggs. Research Project ... involvement of DNA-dependent protein kinase in the phosphorylation of P1 protein in cell-free DNA replication of Xenopus eggs' ...
  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680762/
*  SMART: Secondary literature for NH domain
... including cell-free translation, two-dimensional peptide mapping and recombinant DNA techniques, the structure of the ... which can in part be simulated in the quasi in vivo system of the Xenopus laevis oocyte. The preprohormones to vasopressin and ... Thus, synthetic oligonucleotides consisting of as few as eight nucleotides can be used to prime reverse transcription of ... DNA probes specific for vasopressin or oxytocin mRNAs have been used to identify both classic (hypothalamic) and novel (thymus ...
  http://smart.embl.de/smart/show_secondary.cgi?domain=NH
*  'peptidyl trna' Protocols and Video...
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology', 'Fluorescence- ... Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse ... Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids', ' ... Embryonic Stem Cells', 'Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in ...
  https://www.jove.com/keyword/peptidyl-trna
*  Genesis Special Issue - Xenopus: Advances and Emerging Technologies
... we describe the marriage of Xenopus cell-free cytoplasm and confinement technologies to generate synthetic cell-like systems, ... CELL BIOLOGY. Xenopus egg extract to study regulation of genome-wide and locus-specific DNA replication. Erica Raspelli, Lucia ... The cell-free Xenopus laevis egg extract represents an ideal system to overcome survival issues and to facilitate the ... Cell-free cytoplasmic extracts prepared from Xenopus eggs and embryos have for decades provided a biochemical system with which ...
  http://www.xenbase.org/entry/doNewsRead.do?id=257
*  'bacteriophage t7' Protocols and Video...
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology', 'Synthesis of ... Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus', 'Alphavirus Transducing System: Tools for ... Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay', 'Nuclear Magnetic Resonance ... coli-based Cell-free Expression System', 'Rescue of Recombinant Newcastle Disease Virus from cDNA', 'Expression, Isolation, and ...
  https://www.jove.com/keyword/bacteriophage+t7
*  Marine Drugs | Free Full-Text | Novel Conopeptides of Largely Unexplored Indo Pacific Conus sp. | HTML
... but are present in different cell types (neurons, neuro-endocrine cells, skeletal muscle cells, heart cells); and they possess ... Complementary DNA (cDNA) encoding the NaV channels was subcloned into the corresponding vector: the α-subunits rNaV1.1/pLCT1 ( ... Preparation of RNA for Functional Testing in Xenopus Oocytes. The RNA preparation of the different NaV, KV channels and nAChRs ... Green, B.R.; Bulaj, G. Oxidative folding of conotoxins in immobilized systems. Protein Pept. Lett. 2006, 13, 67-70. [Google ...
  http://www.mdpi.com/1660-3397/14/11/199/htm
*  Recombinant Gene Expression Protocols | SpringerLink
Synthetic Two-Cistron Expression System Brigitte E. Schoner. Pages 89-97 * Expression of cDNA-Encoded Proteins by Cell-Free ... Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer ... Expression of Exogenous Genes in Xenopus Oocytes, Eggs, and Embryos Koichiro Shiokawa, Chie Koga, Yuzuru Ito, Mikihito Shibata ... Expression in Mammalian Cells. * Overview of Vector Design for Mammalian Gene Expression ...
  https://link.springer.com/book/10.1385/0896034801
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cell-free expression, in vitro, transcription-translation, cell-free protein synthesis, synthetic biology, systems biology, ... such as mRNA and DNA degradation rates, can also be adjusted.7 The TX-TL cell-free expression system has been demonstrated for ... Using Xenopus oocytes as expression system, we determine the amount of Rb+ (Li+) transported into the cells by measuring ... Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology ...
  https://www.jove.com/visualize/abstract/21124924/bridging-gap-linking-molecular-simulations-systemic-descriptions
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Xenopus laevis egg extracts are emerging as a powerful cell-free extract model system in DNA damage checkpoint research. Low- ... However, the loss of both pathways is deleterious and results in synthetic lethality or sickness (i.e. slow growth). Conversely ... to prepare cell-free egg extracts (LSE), (2) to treat Xenopus sperm chromatin with two different DNA damaging approaches (MMS ... This cell line provides a powerful tool to investigate protein function in vivo by reverse genetics in vertebrate cells1. DNA ...
  https://www.jove.com/visualize/abstract/23824283/unligated-okazaki-fragments-induce-pcna-ubiquitination-requirement
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
DNA content in cells can be readily quantitated by flow cytometry of cells stained with propidium iodide, a fluorescent DNA ... Xenopus laevis egg extract is a well-characterized, robust system for studying the biochemistry of diverse cellular processes. ... NCs associated to the cells and/or transported to the underlying chamber can be detected. Measurement of free 125I allows ... To examine calcium dynamics, synthetic fluorescent Ca2+ indictors have become popular. Here we demonstrate TED (= targeted- ...
  https://www.jove.com/visualize/abstract/24155894/natriuretic-peptide-receptor-c-agonist-attenuates-expression-cell
*  Kits and methods for generating 5′ capped RNA - CellScript, LLC
... the cell-free extract is a human reticulocyte lysate. In some embodiments, the cell-free extract is synthetic (e.g., not ... a DNA polymerase. One embodiment of a method comprises contacting the uncapped RNA with capping enzyme system and the cap ... the eukaryotic cell in the kit is an oocyte (e.g., a Xenopus oocyte), or a somatic cell of any type from a human, an animal, a ... cell-free extract (e.g., eukaryotic cell-free extract from plant, animal or yeast cells). In some embodiments, the cell-free ...
  http://www.freepatentsonline.com/8846348.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Using Xenopus oocytes as expression system, we determine the amount of Rb+ (Li+) transported into the cells by measuring ... When cells contain loxP sites and express the Cre recombinase, a recombination event occurs. Double-stranded DNA is cut at both ... The dynamics of free calcium in the ER are imaged in one color, while the corresponding ER structure appears in red. At the ... To examine calcium dynamics, synthetic fluorescent Ca2+ indictors have become popular. Here we demonstrate TED (= targeted- ...
  https://www.jove.com/visualize/abstract/19956593/the-snf2-family-member-fun30-promotes-gene-silencing-heterochromatic
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
This system combines siRNA mediated gene silencing and live cell Ca2+ imaging13. Cells are treated with siRNAs that are ... Environmental Sciences, Issue 86, Plant Leaves, Synthetic Biology, Plants, Genetically Modified, DNA, Plant, RNA, Gene ... Mitochondria purified by this method are suitable for cell-free reconstitution of essential mitochondrial processes and can be ... studied extensively in Xenopus oocytes1 and genetically tractable organisms such as yeast2 and the Drosophila derived S2 cell ...
  https://www.jove.com/visualize/abstract/22563492/alternative-splicing-spg7-gene-involved-hereditary-spastic-paraplegia
*  Protocols and Video Articles Authored by Katharine S. Ullman (Translated to Swedish)
Thus, this cell-free system can be used to decipher events involved in mitotic remodeling of the nuclear envelope such as ... including the production of Xenopus egg extract and sperm chromatin DNA. ... We next designed synthetic RNA oligonucleotides to systematically delineate the features within this minimal RNA fragment that ... Feb, 2006 , Pubmed ID: 16364623 The membrane system that encloses genomic DNA is referred to as the nuclear envelope. However, ...
  https://www.jove.com/author/Katharine+S._Ullman?language=Swedish
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Xenopus laevis egg extracts are emerging as a powerful cell-free extract model system in DNA damage checkpoint research. Low- ... However, the loss of both pathways is deleterious and results in synthetic lethality or sickness (i.e. slow growth). Conversely ... to prepare cell-free egg extracts (LSE), (2) to treat Xenopus sperm chromatin with two different DNA damaging approaches (MMS ... The LacI reporter system is carried in all cells, including stem/progenitor cells and can easily be recovered and used to ...
  https://www.jove.com/visualize/abstract/22291963/histone-chaperone-asf1-plays-an-essential-role-maintaining-genomic
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Using Xenopus oocytes as expression system, we determine the amount of Rb+ (Li+) transported into the cells by measuring ... Endothelial cells, high-throughput, DNA, genomic DNA, RT-PCR, PCR ... The dynamics of free calcium in the ER are imaged in one color ... To examine calcium dynamics, synthetic fluorescent Ca2+ indictors have become popular. Here we demonstrate TED (= targeted- ... muscle progenitor cells, biophysical stimulation, iPS cells, myoblasts, muscle tissue, soft tissue, stem cells, cell culture, ...
  https://www.jove.com/visualize/abstract/20634947/frog-alpha-beta-ryanodine-receptors-provide-distinct-intracellular
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
First, bacterial DNA is extracted along with host tissue DNA from clinical biopsies or slit skin smears (SSS).10 The desired ... Since then, these stem cells have been renamed as Adipose-derived Stem Cells or ASCs and have gone on to become one of the most ... This is a convenient model system to directly explore BCL-2 family function because the protein and lipid components are ... Here, we outline an experimental procedure for measuring the thermodynamic property of the Gibbs free energy of unfolding in ...
  https://www.jove.com/visualize/abstract/22073310/association-genetic-loci-with-blood-lipids-chinese
*  Patent US7910370 - Methods of using BCL-2 for the therapeutic treatment and prevention of diseases - Google Patents
The method includes increasing the activity of Bcl-2 in a population of cells and transplanting the population of cells having ... Also provided is a method of prolonging the in vivo survival of transplanted cells for the treatment of a disease or ... A method to enhance the sensitivity of malignant cells to therapy is provided that includes decreasing the activity of Bcl-2 in ... The method includes increasing the activity of Bcl-2 in cells affected by the disease or pathological condition. Diseases or ...
  http://www.google.com/patents/US7910370?dq=6650327
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... coli cells and in vitro in a cell-free system. Sucrose gradient centrifugation is further utilized to isolate RNCs. The ... In this assay, cells are microinjected with either in vitro synthesized mRNA or plasmid DNA containing the gene of interest. ... Using the synthetic modified mRNA, cells can be induced to transiently express the desired proteins, which they normally would ... RNA localization is a conserved mechanism of establishing cell polarity. Vg1 mRNA localizes to the vegetal pole of Xenopus ...
  https://www.jove.com/visualize/abstract/22844456/tristetraprolin-inhibits-poly-tail-synthesis-nuclear-mrna-that
*  Marine Drugs | Free Full-Text | Biogenetic Relationships of Bioactive Sponge Merotriterpenoids | HTML
Cell 2000, 11, 3495-3508. [Google Scholar] [CrossRef] [PubMed]. *Dictenberg, J.B.; Swanger, S.A.; Antar, L.N.; Singer, R.H.; ... The toxicols (17-19) and shaagrockol C (22) inhibit the DNA polymerase function of HIV-1 reverse transcriptase [3]. Akaterpin ( ... Formation of nuclear pore complexes (NPCs) in Xenopus laevis eggs was inhibited by 2, but not the double membrane of the ... Biosynthesis of Diazepinomicin/ECO-4601, a Micromonospora Secondary Metabolite with a Novel Ring System. J. Nat. Prod. 2008, 71 ...
  http://www.mdpi.com/1660-3397/15/9/285/htm
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cells grown in 3D retain the important cues from the extracellular matrix and provide a physiologically relevant ex vivo system ... Xenopus egg extract has been used to study protein turnover in many cellular contexts, including the cell cycle and signal ... Post-EMT AV cushion mesenchymal cells can be cultured inside mechanically constrained or stress-free collagen gels. These 3D in ... Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells ...
  https://www.jove.com/visualize/abstract/24944076/the-anti-tumor-efficiency-pterostilbene-is-promoted-with-combined
*  Cell culture - Wikipedia
... they are model system of plant cell) Other species cell lines Dog MDCK kidney epithelial Xenopus A6 kidney epithelial Zebrafish ... Biological products produced by recombinant DNA (rDNA) technology in animal cell cultures include enzymes, synthetic hormones, ... 3D cell cultures can be grown using a scaffold or matrix, or in a scaffold-free manner. Scaffold based cultures utilize an ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ...
  https://en.wikipedia.org/wiki/Cell_culture
*  Marine Drugs | Free Full-Text | APETx4, a Novel Sea Anemone Toxin and a Modulator of the Cancer-Relevant Potassium Channel...
... sea anemone fractions were screened for inhibitory activity on KV10.1 by measuring whole-cell currents as expressed in Xenopus ... APETx4 induces a concentration-dependent cytotoxic and proapoptotic effect in various cancerous and noncancerous cell lines. ... This dye is unable to enter viable cells but binds DNA in (or released from) dead cells, thereby enhancing its green ... Figure S6 provides images taken by the IncuCyte Zoom System for each cell line at 0 h, 24 h and 48 h after changing the growth ...
  http://www.mdpi.com/1660-3397/15/9/287/htm
*  Barry Aprison « People « Institute for Genomics and Systems Biology
... serum-free cultures of Xenopus laevis parenchymal liver cells. Dr. Aprison taught undergraduates biology and received his Ph.D ... They include classes for Systems Biology & Disease (MD-Ph.D. students), Systems & Networks in Cancer (grad students), Synthetic ... At Brandeis University he investigated the regulation of yolk protein gene expression and DNA synthesis in fully-defined, ... Institute for Genomics & Systems Biology. Knapp Center for Biomedical Discovery. 900 East 57th Street. Chicago, IL 60637 ...
  http://www.igsb.anl.gov/people/barry-aprison/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Here, we describe one such assay using a plant cell-free system. This assay can be adapted for studies of the roles of ... alkane hydroxylase system, resting cell assay, prefoldin, Escherichia coli, synthetic biology, homologous interaction mapping, ... Xenopus egg extract has been used to study protein turnover in many cellular contexts, including the cell cycle and signal ... To be able to monitor ubiquitin-proteasome activity in living primary cells, complementary DNA constructs coding for a green ...
  https://www.jove.com/visualize/abstract/26186340/proteasome-activity-is-affected-fluctuations-insulin-degrading-enzyme