Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. - Nuffield...
Washing samples results in decreased AnnV+MPs (P=0.002) and platelet-derived MPs (PMPs) (P=0.002). Double centrifugation of MPs ... Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated levels are found ... Cell-Derived Microparticles, Endothelial Cells, Flow Cytometry, Humans, Leukocytes, Middle Aged, Platelet Endothelial Cell ... Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. ...https://www.wrh.ox.ac.uk/publications/314642
Microparticles Carrying Peroxisome Proliferator‐Activated Receptor Alpha Restore the Reduced Differentiation and Functionality...
Derived Cells Induced by High‐Fat Diet. ...https://insights.ovid.com/pubmed?PMID=29080294
ISAC-NET - Microvesicle Analysis Interest Development Group
Jy, W., et al., Measuring circulating cell-derived microparticles. J Thromb Haemost, 2004. 2(10): p. 1842-51.. Other Resources ... Ayers, L., et al., Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the ... Super-resolved calibration-free flow cytometric characterization of platelets and cell-derived microparticles in platelet-rich ... Cell-derived Membrane Vesicles. Mammalian cells release small membrane vesicles that can carry biological molecules and signals ...http://isac-net.org/Resources/Microvesicle-Analysis-Interest-Development-Group.aspx
Physical activity, immune function and inflammation in kidney patients (the PINK study): a feasibility trial protocol | BMJ Open
Measuring circulating cell-derived microparticles. J Thromb Haemost 2004;2:1842-3.doi:10.1111/j.1538-7836.2004.00936.x. ... neutrophil derived (CD66b+), monocyte derived (CD14+) and endothelial cell derived (CD144+). Prothrombotic potential will be ... A flow cytometric method for characterization of circulating cell-derived microparticles in plasma. J Extracell Vesicles 2014;3 ... T cells will be categorised as helper (CD3+CD4+) cytotoxic (CD3+CD8+) and regulatory (CD4+CD25+CD127-) T cells. B Cells will be ...http://bmjopen.bmj.com/content/7/5/e014713
Circulating Endothelial-Derived Activated Microparticle: A Useful Biomarker for Predicting One-Year Mortality in Patients with...
A Useful Biomarker for Predicting One-Year Mortality in Patients with Advanced Non-Small Cell Lung Cancer. Chin-Chou Wang,1,2,3 ... platelet-derived apoptotic MPs (PDAp-MPs), (3) endothelial-derived activated MPs (EDAc-MPs), and (4) endothelial-derived ... This study tested the hypothesis that circulating microparticles (MPs) are useful biomarkers for predicting one-year mortality ... Levels of four MPs in circulation, (1) platelet-derived activated MPs (PDAc-MPs), (2) ...https://www.hindawi.com/journals/bmri/2014/173401/abs/
Adenovirus-induced thrombocytopenia: the role of von Willebrand factor and P-selectin in mediating accelerated platelet...
New horizons in the analysis of circulating cell-derived microparticles. Keio J Med 2004; 53:210-230. ... There is an associated increase in platelet and leukocyte-derived microparticles. Adenovirus-induced endothelial cell ... and we have also documented an increased number of circulating endothelial cell-derived MPs. Endothelial cell activation and ... Microparticles (MPs) are vesiculations of plasma membranes that are released from cells during activation, stress, and ...http://www.bloodjournal.org/content/109/7/2832?ijkey=49933bdd033494304eb4d8ab7bec94eaa9c4e4e0&keytype2=tf_ipsecsha&sso-checked=true
Vesicles Archives | Page 9 of 9 | Izon Science
Cell-derived or extracellular vesicles, including microparticles and exosomes, are abundantly present in body fluids such as ... Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical ... Microvesicles (MVs) are cell-derived vesicles which are of interest in a clinical setting, as they may be predictive of early ... Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes. In by Jules van der Voorn. ...http://izon.com/pub-application/vesicles/page/9/
Publication Izon Science have submitted or been involved with » Izon Science
Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical ... These data may necessitate reevaluation of certain published data on patient-derived MPs and contribute to correct the clinical ... Numerous diseases, recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been ...http://www.izon.com/publications/publication/159
Similar articles for PubMed (Select 23601226) - PubMed - NCBI
Commentary on: "predicting carotid artery disease and plaque instability from cell-derived microparticles". ...https://www.ncbi.nlm.nih.gov/pubmed?linkname=pubmed_pubmed&from_uid=23601226
The antiphospholipid syndrome: from pathophysiology to treatment, Clinical and Experimental Medicine | 10.1007/s10238-016-0430...
Cell-derived microparticles and exosomes in neuroinflammatory disorders. Horstman, LL; Jy, W; Minagar, A ... Circulating platelet-derived microparticles in systemic lupus erythematosus. Association with increased thrombin generation and ... Significance of anti-oxidized LDL antibody and monocyte-derived microparticles in anti-phospholipid antibody syndrome ... Endothelial microparticle release is stimulated in vitro by purified IgG from patients with the antiphospholipid syndrome ...https://www.deepdyve.com/lp/springer_journal/the-antiphospholipid-syndrome-from-pathophysiology-to-treatment-QBt95f2ejj
Inflammatory Disorders of the Nervous System | Springer for Research & Development
Circulating Cell-Derived Microparticles in Thrombotic and Inflammatory Disorders Wenche Jy, Lawrence L. Horstman, Joaquin J. ... "endothelial and leukocyte microparticles" and "gene microarray technology" to help explain important links between the central ...https://rd.springer.com/book/10.1385/1592599052
Cyclooxygenase-2-Derived Prostacyclin Regulates Arterial Thrombus Formation by Suppressing Tissue Factor in a Sirtuin-1...
Hematopoietic cell-derived microparticle tissue factor contributes to fibrin formation during thrombus propagation. Blood. 2004 ... Induction of microparticle- and cell-associated intravascular tissue factor in human endotoxemia. Blood. 2004;103:4545-4553. ... Cell Culture and Isolation of Total Leukocytes. Mouse cardiac endothelial cells (MCECs) were cultured as previously described. ... Plasma Microparticle Isolation. Microparticles were obtained from platelet-poor plasma diluted 1:3 with 20 mmol/L HEPES and 1 ...http://circ.ahajournals.org/content/126/11/1373
Table of Contents | Blood Journal
Hematopoietic cell-derived microparticle tissue factor contributes to fibrin formation during thrombus propagation Janet Chou, ... Anaplastic large cell lymphomas lack the expression of T-cell receptor molecules or molecules of proximal T-cell receptor ... Gene expression profiling of normal and malignant CD34-derived megakaryocytic cells Elena Tenedini, Maria Elena Fagioli, Nicola ... NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs Christophe ...http://www.bloodjournal.org/content/104/10?sso-checked=true
Tissue factor-positive neutrophils bind to injured endothelial wall and initiate thrombus formation | Blood Journal
Cancer cell-derived microparticles bearing P-selectin glycoprotein ligand 1 accelerate thrombus formation in vivo. J Exp Med ... Hematopoietic cell-derived microparticle tissue factor contributes to fibrin formation during thrombus propagation. Blood 2004; ... Trans-cellular migration: cell-cell contacts get intimate. Curr Opin Cell Biol 2008;20(5):533-540. ... and dendritic cells (DCs) are all fluorescently labeled.27 Exogenous monocytes and monocyte-derived microparticles were ...http://www.bloodjournal.org/content/120/10/2133?ijkey=76fa6d8aa89c00e993e5276bcd3c62e32d34d156&keytype2=tf_ipsecsha&sso-checked=true
... surface fragments of apoptotic cells, advanced glycated proteins, cell derived microparticles, and others (Silverstein et al., ... 2008) Platelet CD36 mediates interactions with endothelial cell-derived microparticles and contributes to thrombosis in mice. J ... endothelial cell cultures were placed in cell culture medium without puromycin. Endothelial cells were cultured until the cells ... To address this issue, we used flow cytometry to assess the accumulation of BM-derived cells at 24 and 72 h after ischemia in ...http://www.jneurosci.org/content/35/44/14783
Nouvelle Cuisine: Platelets Served with Inflammation | The Journal of Immunology
Improved flow cytometric assessment reveals distinct microvesicle (cell-derived microparticle) signatures in joint diseases. ... Platelet-derived CD40L also was shown to enhance CD8+ T cell responses and to promote T cell responses postinfection with ... Functionally, platelet microparticles (PMPs) are thought to participate in cell-cell communication. The PMP cargo is vast and ... suggesting that these proteins are involved in microparticle clearance and in microparticle interaction with other cells (131, ...http://www.jimmunol.org/content/194/12/5579
Aggregated Low-Density Lipoprotein Uptake Induces Membrane Tissue Factor Procoagulant Activity and Microparticle Release in...
Phospholipid composition of cell-derived microparticles determined by one-dimensional high-performance thin-layer ... Arrested cells were incubated with nLDL or agLDL for 2, 4, 6, 8, 12, 24, and 48 hours. At each time point, cells were washed ... High-Performance Thin-Layer Chromatography Analysis of Phospholipid Composition of VSMC Membrane and Cell-Derived MPs. Arrested ... Tedgui A, Mallat Z. Smooth muscle cells: another source of tissue factor-containing microparticles in atherothrombosis? Circ ...http://circ.ahajournals.org/content/110/4/452
Volume 21 Issue 11 | Journal of Biomedical Optics
Flow cytometry method (FCM) is widely used for analysis of cell-derived microparticles (MPs). Numerous efforts are currently ... Data derived from human embryonic kidney cells (HEK293T) indicate that when driven by a heat-shock promoter, MSNP cargo ... Raman microscopy of bladder cancer cells expressing green fluorescent protein Gurjit Mandair, Amy Han, Evan Keller, Michael ... Light-scattering gating and characterization of plasma microparticles Anastasiya Konokhova, Darya Chernova, Dmitry Strokotov, ...https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-21/issue-11
Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation. R J Berckmans. Department of ... derived from damaged cells at these sites will become exposed to blood components and could contribute to blood coagulation and ... Cell 53:505-18. 1988. *. Role of platelet P-selectin and microparticle PSGL-1 in thrombus formation. Bruce Furie. Center for ... Conversely, activated coagulation proteases may affect specific cellular receptors on inflammatory cells and endothelial cells ...https://www.labome.org/topics/physical/natural/biological/circulatory/blood/blood/hemostasis/blood-coagulation-19993.html
Abgeschlossene Projekte (US und Deutschland)- Institut für Translationale Immunologie (TIM)
NIH 1R21DK075857-01A2. Title: "Fibrolytic activation of hepatic stellate cells by T cell derived microparticles". Funktion: ...http://www.unimedizin-mainz.de/tim/forschung/drittmittelprojekte/abgeschlossene-projekte-us-und-deutschland.html
AM-derived SOCS attenuates pulmonary STAT activation in | Open-i
AM-derived SOCS attenuates pulmonary STAT activation in vivo. (A-D) Mouse lungs were pretreated oropharyngeally with 50 µl ... Cell-Derived Microparticles/immunology*/pathology. *Epithelial Cells/immunology*/pathology. *Macrophages/immunology*. * ... 7 E). In contrast to the effects of AM-derived MPs, administration of the same number of rat PM-derived MPs, isolated from CM ... 7 E). In contrast to the effects of AM-derived MPs, administration of the same number of rat PM-derived MPs, isolated from CM ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4419346_JEM_20141675_Fig7&req=4
Factor V Leiden and Inflammation
P. Chamouard, D. Desprez, B. Hugel et al., "Circulating cell-derived microparticles in Crohn's disease," Digestive Diseases and ... R. L. Silverstein, "Activation of Vascular Cells by Microparticles and Other Danger Signals Via the CD36 Scavenger Receptor ( ... A. Andoh, T. Tsujikawa, K. Hata et al., "Elevated circulating platelet-derived microparticles in patients with active ... D. C. Altieri, "Inflammatory cell participation in coagulation," Seminars in Cell and Developmental Biology, vol. 6, no. 5, pp ...https://www.hindawi.com/journals/thrombosis/2012/594986/ref/
WikiGenes - SELP - selectin P (granule membrane protein...
Endothelial cell-derived microparticles in allogeneic hematopoietic stem cell recipients. Pihusch, V., Rank, A., Steber, R., ... Granule membrane protein 140 (GMP140) binds to carcinomas and carcinoma-derived cell lines. Aruffo, A., Dietsch, M.T., Wan, H ... Development and assessment of enzyme immunoassay for platelet-derived microparticles. Osumi, K., Ozeki, Y., Saito, S., Nagamura ... CD62/P-selectin recognition of myeloid and tumor cell sulfatides. Aruffo, A., Kolanus, W., Walz, G., Fredman, P., Seed, B. Cell ...http://www.wikigenes.org/e/gene/e/6403.html
Cellular microparticles and pathophysiology of traumatic brain injury | SpringerLink
The finding that cellular microparticles (MPs) generated by injured cells profoundly impact on pathological courses... ... Abbreviations: BDMP, brain-derived microparticles; CSF, cerebrospinal fluid; EMP, endothelial-derived microparticles; EV, ... leukocyte-derived microparticles; miR, micro RNA; MP, microparticles; mtMP, mitochondrial miroparticles; PMP, platelet-derived ... Horstman LL, Jy W, Jimenez JJ, Bidot C, Ahn YS (2004) New horizons in the analysis of circulating cell-derived microparticles. ...https://link.springer.com/article/10.1007/s13238-017-0414-6
Flow Cytometry of Extracellular Vesicles: Potential, Pitfalls, and Prospects - Current Protocols
Measuring circulating cell‐derived microparticles. J. Thromb. Haemost. 2:1842‐1851.. Lacroix, R., Dubois, C., Leroyer, A.S., ... Measurement of circulating cell‐derived microparticles by flow cytometry: Sources of variability within the assay. Thromb. Res ... Flow cytometry has been a popular tool for measuring cell‐derived EVs, but has often been used in an uncritical manner in which ... Standardization of platelet‐derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-cy1314.html?quicktabs_cp=abstract