NF-κB Inhibition by Bortezomib Permits IFN-γ-Activated RIP1 Kinase-Dependent Necrosis in Renal Cell Carcinoma | Molecular...
The VHL gene, a key tumor suppressor in ccRCCs (27), was not mutated in any of the 3 cell lines (not shown), and its product- ... extracts from 2 populations of cultured kidney epithelial cells (NKC1 and NKC2) were used. C, whole-cell extracts from RCC cell ... The role of von Hippel-Lindau tumor suppressor protein and hypoxia in renal clear cell carcinoma. Am J Physiol Renal Physiol ... Frequent mutations of genes encoding ubiquitin-mediated proteolysis pathway components in clear cell renal cell carcinoma. Nat ...http://mct.aacrjournals.org/content/12/8/1568
BAP1 - Wikipedia
Mutations in the tumor suppressor gene BAP1 occur in approximately 15% of clear cell renal cell carcinoma (CCRCC) cases. ... but BAP1 was first shown to act as a tumor suppressor in cultured cells, where its deubiquitinase (UCH) domain and Nuclear ... In cancer, BAP1 can function both as a Tumor suppressor and as a Metastasis suppressor. BAP1 somatic mutations were identified ... Sequencing efforts demonstrated worse outcomes in patients with BAP1 mutated clear cell renal cell carcinoma. . Two studies ...https://en.wikipedia.org/wiki/BAP1
β-Catenin Activation and Epithelial-Mesenchymal Transition in the Pathogenesis of Pterygium | IOVS | ARVO Journals
Overexpression of p53 tumor suppressor gene in pterygia. Eye. 2002;16:619-621. [CrossRef] [PubMed] ... Expression of vimentin by rabbit corneal epithelial cells during wound repair. Cell Tissue Res. 1992;267:347-356. [CrossRef] [ ... The role of epithelial-to-mesenchymal transition in renal fibrosis. J Mol Med. 2004;82:175-181. [CrossRef] [PubMed] ... Growth factors in cultured pterygium fibroblasts: immunohistochemical and ELISA analysis. Graefes Arch Clin Exp Ophthalmol. ...https://iovs.arvojournals.org/article.aspx?articleid=2126221
MicroRNA‑133b inhibits cell migration and invasion by targeting matrix metalloproteinase 14 in glioblastoma
Wu et al showed that miR-133b suppressed the cell proliferation, migration and invasion of renal cell carcinoma cells (9). ... This result demonstrates that miR-133b acts as a tumor suppressor and inhibits GBM cell migration and invasion. ... The cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Hyclone, Logan, UT, USA) containing 100 mg/ml streptomycin ... Western blotting and a luciferase reporter assay were used to identify the target genes of miR‑133b. It was found that miR‑133b ...https://spandidos-publications.com/ol/10/5/2781
An Association Study on Genetic Polymorphisms of Rab37 Gene with the Risk of Esophageal Squamous Cell Carcinoma in a Chinese...
... renal cell carcinoma; NSCLC: non-small cell lung cancer; TSG: tumor suppressor gene; EA: esophageal adenocarcinoma; SNPs: ... Involvement of TMEM22 overexpression in the growth of renal cell carcinoma cells. Oncol Rep. 2009;21:305-12 ... EA.hy926, HepG2, MCF7 and SW1990 were from American Type Culture Collection (ATCC). All cell lines were cultured in Dulbecco's ... Then, Rab37 was regarded as a metastasis-related tumor suppressor gene (TSG) in lung cancer . Except the two reports, there ...http://www.medsci.org/v10p0235.htm
DNA Fragments in the Blood Plasma of Cancer Patients: Quantitations and Evidence for Their Origin from Apoptotic and Necrotic...
... the presence of oncogene or tumor suppressor gene mutations that characterize DNA in tumor cells were detected in plasma DNA. ... small cell lung carcinomas, or renal carcinomas, could be determined in patients' plasma DNA (7, 8, 9) . Furthermore, tumor- ... a conclusion that we support by studies using cultured cells and mice with in vivo-induced apoptosis and necrosis. ... Plasma DNA can originate from both tumor and nontumor cells. We found that neither T cells nor endothelial cells seem to ...http://cancerres.aacrjournals.org/content/61/4/1659.long
Indoxyl sulfate (IS) and p-cresyl sulfate (PCS) increas | Open-i
Cultured human renal tubular HK2 cells treated with these toxins were used as an in vitro model. Injections of indoxyl sulfate ... Recent studies suggest that Klotho may be a tumor suppressor, and its expression may be repressed by DNA hypermethylation in ... Thus, inhibition of Klotho gene expression by uremic toxins correlates with gene hypermethylation, suggesting that epigenetic ... Cultured human renal tubular HK2 cells treated with these toxins were used as an in vitro model. Injections of indoxyl sulfate ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3306006_ki2011445f7&req=4
Interactive Fly, Drosophila
Expression of the wild-type TSC2 gene in TSC2 mutant tumor cells inhibits proliferation and tumorigenicity. This 'suppressor' ... In the Eker rat, however, a germ-line Tsc2 mutation gives rise to renal cell carcinomas with a complete penetrance. The ... The angiofibroma stroma cells of three adult TSC patients were cultured and these cells were compared with normal skin ... LKB1 (Drosophila homolog: lkb1), the gene mutated in PJS, acts as a tumor suppressor by activating TSC2, the gene mutated in ...http://www.sdbonline.org/sites/fly/newgene/gigas2.htm
MicroRNA‑495 suppresses cell proliferation and invasion of hepatocellular carcinoma by directly targeting insulin‑like growth...
Zhang B, Yuan F, Liu J, Li Y, Zhou F, Liu X, Hao Z, Li Q, Zheng Y and Wang W: Hsa-miR-495 acts as a tumor suppressor gene in ... MicroRNA-497 suppresses renal cell carcinoma by targeting VEGFR-2 in ACHN cells. Biosci Rep. 37:pii: BSR201702702017. View ... All HCC cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS; ... Shanghai, China). For cell transfection, cells were seeded into 6-well plates at a density of 5×105 cells per well. After ...https://www.spandidos-publications.com/etm/15/1/1150
MicroRNA-708 Induces Apoptosis and Suppresses Tumorigenicity in Renal Cancer Cells | Cancer Research
... as an important tumor suppressor in renal cell carcinoma (RCC). miR-708 expression was attenuated widely in human RCC specimens ... or loss of epigenetic silencing of a gene encoding a miRNA that targets one or more tumor suppressor genes could inhibit the ... UCSF Cell Culture Facility). All cell lines were cultured in a humidified incubator (5% CO2) at 37ºC. ... Cell lines and cell culture. The nonmalignant SV-40 immortalized renal cell line HK2 and human renal cancer cell lines A498 and ...http://cancerres.aacrjournals.org/content/71/19/6208
Protocols and Video Articles Authored by Nathalie Arbour (Translated to Arabic)
von Hippel-Lindau clear cell RCC (VHL(-/-)RCC) are characterized by mutations in the VHL tumor suppressor gene. Loss of VHL ... of T cell lines was assessed by comparing their proliferation and IFN-gamma secretion when cultured with antigen-loaded B cells ... TGF-alpha As a Candidate Tumor Antigen for Renal Cell Carcinomas Cancer Immunology, Immunotherapy : CII. Aug, 2009 , Pubmed ID ... CD8+ T cells, and gamma/delta T cells. NKG2D ligands have been detected on many tumor cell types and can be induced on ...https://www.jove.com/author/Nathalie_Arbour?language=Arabic
Discovery of a novel TLR2 signaling inhibitor with anti-viral activity
As 786-O and 769-G cells possess VHL mutation in each different system , we chosen the two renal tumor cell lines in our ... About 70% of very clear cell RCC features mutation or inactivation GP3A of the VHL growth suppressor gene. ... In addition, cells cultured on GRGDSP(FN) covered areas had been verified by immunophenotyping using anti-CD105 PE, Compact ... Louis, MO). Cell ethnicities and lines The two renal tumor cell lines, 769-G and 786-O (bought from American Type Tradition ...http://research-matters.net
The effects of wild-type p53 tumor suppressor activity and mutant p53 gain-of-function on cell growth. Gene 2001; 277: 15-30. ... and colon carcinoma cell line HCT-116, as well as its p53-deficient variant HCT-116/p53−/−. All cells were cultured at 37°C in ... The cell lines investigated included renal carcinoma (RCC26b, RCC45, RCC54, and ACHN), osteosarcoma (U2OS), lung fibrosarcoma ( ... Mitotic catastrophe and endomitosis in tumor cells: an evolutionary key to a molecular solution. Cell Biol Int 2005; 29: 1012-8 ...http://cancerres.aacrjournals.org/content/67/21/10351
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Tumor Cells, Cultured Colonic Neoplasms, Primary Cell Culture, Colon tumor, chelation, collagenase, matrigel, organoid, EGF, ... resulting in expression of mutated oncogenes and deletion of tumor suppressor genes. The phenotypic consequences of these ... This protocol describes a set of labeling techniques that can be used to gauge renal composition and test nephron functionality ... colon tumor cells digestion into single cells, embedding colon tumor cells into matrigel, and selective culture based on the ...https://www.jove.com/visualize/abstract/21437251/the-notch-2-gene-is-regulated-wnt-signaling-cultured-colorectal
Insulin-like growth factor binding protein 7 for treatment of cancer - University of Massachusetts
... or suspected of having a tumor, and administering to the subject an effective amount of an IGFBP7 agent if t ... Methods of treating a tumor in a subject include identifying a subject having, at risk for, ... "Epigenetic silencing of the RASSF1A tumor suppressor gene through HOXB3-mediated induction of DNMT3B expression," Mol. Cell., ... SK-MEL-28 cells were incubated with rIGFBP7 for 0, 2, 6, 12, or 24 hours, following which the cells were washed and cultured in ...http://www.freepatentsonline.com/9248163.html
Regulation of the Chemokine Receptor CXCR4 by Hypoxia | JEM
... gene-mediated growth suppression and induction of differentiation in renal cell carcinoma cells grown as multicellular tumor ... Effects of Hyp on CXCR4 expression by cancer cells. The ovarian cancer cell line CAOV3 was cultured in normoxic or hypoxic ... von Hippel-Lindau tumor suppressor protein; SDF-1, stromal-derived factor 1; TAM, tumor-associated macrophage; VEGF, vascular ... human breast cancer cells), CAOV3 (human ovarian cancer cell line), 786.0 (36), and WT2 human renal cancer cells (23) were ...http://jem.rupress.org/content/198/9/1391
A vital role for Angptl3 in the PAN-induced podocyte loss by affecting detachment and apoptosis in vitro | BMC Nephrology |...
Both the tumor suppressor protein p53 and caspases are involved in the crucial processes of renal and nonrenal cell apoptosis [ ... All cells were cultured on six-well plates under nonpermissive conditions for 14 days prior to the experiments, fields of cells ... ANGPTL3 gene transfection ameliorates the PAN-induced podocyte detachment . The experimental methods and conditions in the ... Regulation and function of the p53 tumor suppressor protein. Curr Opin Cell Biol. 2001;13:332-7.View ArticlePubMedGoogle ...https://bmcnephrol.biomedcentral.com/articles/10.1186/s12882-015-0034-4
SEMA3F | Cancer Genetics Web
... adhesion of the tumor cells to plastic or fibronectin nor did they modulate the proliferation of tumor cells cultured in cell ... functions as a tumor suppressor gene in schwannoma cells, the functional role of merlin in the tumor microenvironment and the ... We searched for chromosome 3p homo- and hemizygous losses in 23 lung cancer cell lines, 53 renal cell and 22 breast carcinoma ... cultured melanocytes and melanoma cells, dermal fibroblasts, squamous cell carcinoma cells and keratinocytes. Gene expression ...http://www.cancerindex.org/geneweb/SEMA3F.htm
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
We found that some tumor cell lines such as melanoma B16F1 cells, glioblastoma U87 cells, and breast cancer MDA-MB-435 cells ... resulting in expression of mutated oncogenes and deletion of tumor suppressor genes. The phenotypic consequences of these ... While these cultured tumor stem cells were relatively small, often bipoar with high nuclear to cytoplasmic ratio when cultured ... This protocol describes a set of labeling techniques that can be used to gauge renal composition and test nephron functionality ...https://www.jove.com/visualize/abstract/21731601/specific-syndecan-1-domains-regulate-mesenchymal-tumor-cell-adhesion
Renal cell carcinoma development in the rat independent of alterations at the VHL gene locus. | Scholars@Duke
Renal cell carcinoma development in the rat independent of alterations at the VHL gene locus.'s profile, publications, research ... Tumor Cells, Cultured * Tumor Suppressor Proteins * Ubiquitin-Protein Ligases * Von Hippel-Lindau Tumor Suppressor Protein ... tumor suppressor gene predispose to renal cell carcinoma and a constellation of other tumor types found in VHL disease. This ... spontaneous renal cell carcinoma develops with a high frequency. We therefore investigated the role of this tumor suppressor ...https://scholars.duke.edu/display/pub1143572
Inhibition of CCN6 (WISP3) expression promotes neoplastic progression and enhances the effects of insulin-like growth factor-1...
... cells with a CCN6 hairpin short interfering RNA. CCN6-deficient cells showed increased motility and invasiveness, and developed ... CCN6 is a secreted protein lost in 80% of the aggressive inflammatory breast cancers, and can decrease mammary tumor growth in ... Inhibition of CCN6 expression promoted anchorage-independent growth of HME cells and rendered them more responsive to the ... Specific stable inhibition of CCN6 expression in HME cells induces EMT, promotes anchorage-independent growth, motility and ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1351
Saúde Pública - Environmental exposures and gene regulation in disease etiology Environmental exposures and gene regulation in...
... on the expression of LH receptors during cell differentiation in cultured granulosa cells. Mol Cell Endocrinol 2003; 202(12): ... In other cases, altered methylation of oncogenes or tumor suppressor genes is mediated by dietary folate intake. Folate, found ... Cadmium exposure also potentiates some diabetic complications related to renal tubular and glomerular function51. ... As in the E. coli example, tumor cells can respond to treatment by amplifying and/or mutating genes that promote their survival ...https://scielosp.org/scielo.php?script=sci_arttext&pid=S1413-81232008000100030&lng=en
IJMS | Free Full-Text | Thrombospondin-1 Is a Putative Target Gene of Runx2 and Runx3 | HTML
Expression of TSP-1 is downregulated in cancer cells, and its ectopic expression has been shown to restrain tumor growth. ... is synthesized and secreted by many cell types, including osteoblast and cancer cells. TSP-1 is highly upregulated during early ... Hence, these results provide first line of evidence that TSP-1 is a transcriptional target gene of Runx2 and Runx3. ... On the other hand, TSP-1 gene expression drastically increased at both the transcriptional and translational levels in response ...http://mdpi.com/1422-0067/14/7/14321/htm
Index by author | Cancer Research
Expression of Cytokine Genes, Cytokine Receptor Genes, and Transcription Factors in Cultured Hodgkin and Reed-Sternberg Cells ... Serum Level of Interleukin 6 as a Prognosis Factor in Metastatic Renal Cell Carcinoma ... Effect of Thrombin Treatment of Tumor Cells on Adhesion of Tumor Cells to Platelets in Vitro and Tumor Metastasis in Vivo ... Localization of Metastasis Suppressor Gene(s) for Prostatic Cancer to the Short Arm of Human Chromosome 11 ...http://cancerres.aacrjournals.org/content/52/12.index-by-author
Calcium dobesilate may alleviate diabetes‑induced endothelial dysfunction and inflammation
Control cells were maintained in medium containing 5.5 mmol/l D-glucose to provide the cells with the basal needs for cell ... Dipetrillo K and Gesek FA: Pentoxifylline ameliorates renal tumor necrosis factor expression, sodium retention, and renal ... Navarro JF, Milena FJ, Mora C, León C and García J: Renal pro-inflammatory cytokine gene expression in diabetic nephropathy: ... HG‑treated HUVECs exhibited increased FITC‑BSA permeability compared to control cells cultured in medium alone; however, CaD ...https://www.spandidos-publications.com/mmr/16/6/8635