Site of catabolite inhibition of carbohydrate metabolism." by J F. McGinnis and K Paigen
Cell-Membrane: me, Culture-Media, Enzyme-Repression, Escherichia-Coli: en, gd, me, Galactose, Glucose: me, Glycosides: me, ...https://mouseion.jax.org/stfb1970_1979/400/
Targeting Cellular Metabolism Chemosensitizes the Doxorubicin-Resistant Human Breast Adenocarcinoma Cells
In turn, the reduction in intracellular ATP levels might be potentiated by the repression of the levels of membrane transport ... MCF-7/MDR cells), the effects and mechanisms of 2-deoxy-D-glucose (2DG), a glucose analogue, on reversing multidrug resistance ... Aliquots of cell extract were prepared by ultrasonication. Samples were added to an enzyme reaction mixture (100 mM Tris-HCl ... The cell viability was calculated by the formula ... Cell Line. Human breast carcinoma cell line MCF-7, obtained ...https://www.hindawi.com/journals/bmri/2015/453986/
Catabolite repression - Wikipedia
It works by the fact that Hpr is phosphorylated by a specific mechanism, when glucose enters through the cell membrane protein ... In enteric bacteria one of the EIIA enzymes in their set is specific for glucose transport only. When glucose levels are high ... The catabolite repression was first shown to be initiated by glucose and therefore sometimes referred to as the glucose effect ... Absence of glucose will "turn off" catabolite repression. When glucose levels are low the phosphorylated form of EIIA ...https://en.wikipedia.org/wiki/Catabolite_repression
Frontiers | Multiple Roles of MYC in Integrating Regulatory Networks of Pluripotent Stem Cells | Cell and Developmental Biology
Indeed, PSCs are used for both disease and cancer modeling and to derive cells for regenerative medicine. For these reasons ... Indeed, it instruct the PSC-specific cell cycle, metabolism and epigenetic landscape, contributes to limit exit from ... Indeed, it instruct the PSC-specific cell cycle, metabolism and epigenetic landscape, contributes to limit exit from ... thus determining their cell identity. Among the transcription factors, MYC represents a central hub, which modulates and ...https://www.frontiersin.org/articles/10.3389/fcell.2017.00007/full
In pancreatic β-cells, activation of SREBP-1c has been shown to be involved in impaired insulin secretion and glucose ... implicating a role for SREBP-1c-mediated IRS-2 repression in the palmitate-EPA-mediated changes in β-cell physiology (Fig. 2D; ... 2A). These changes in SREBP-1c mRNA were associated with those in both membrane and nuclear forms of SREBP-1c protein (Fig. 2D ... for insulin measurement with an insulin enzyme-linked immunosorbent assay kit. Hoechst-33258 was used to determine the DNA ...http://diabetes.diabetesjournals.org/content/57/9/2382
Hydrogen Sulfide Alleviates Postharvest Senescence of Grape by Modulating the Antioxidant Defenses
LOX, as one of the key enzymes in membrane lipid peroxidation, is capable of catalyzing the peroxidation of unsaturated fatty ... role of H2S in grapes could be attributed to the increased activities of ROS-scavenging enzymes which bring about a repression ... P. Gao, Z. Zhu, and P. Zhang, "Effects of chitosan-glucose complex coating on postharvest quality and shelf life of table ... C. García-Mata and L. Lamattina, "Hydrogen sulphide, a novel gasotransmitter involved in guard cell signalling," New ...https://www.hindawi.com/journals/omcl/2016/4715651/
PEP group translocation - Wikipedia
EIIB phosphorylates glucose as it crosses the plasma membrane through the transmembrane Enzyme II C (EIIC), forming glucose-6- ... The benefit of transforming glucose into glucose-6-phosphate is that it will not leak out of the cell, therefore providing a ... which is involved in the catabolite repression system, also known as glucose effect. When the glucose concentration is high, ... For example, at low glucose concentrations phosphorylated EIIA accumulates and this activates membrane-bound adenylate cyclase ...https://en.wikipedia.org/wiki/PEP_group_translocation
MicroRNAs as stress regulators in pancreatic beta cells and diabetes
... compared to MIN6 cells and dissociated β-cells), where it may already attain maximal repression of target genes responsible for ... which facilitates delivery in cells by associating with lipoproteins and binding to cell surface membrane receptors such as the ... Studies of β-cell specific miR-7aKO mice also exhibited increased glucose tolerance and insulin secretion in response to an ... Conditional deletion of the miRNA processing enzyme Dicer has enabled the study of reduced or absent miRNA activity in a cell ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5605735/
Antievolution.org - Antievolution.org Discussion Board -Topic::ATPase origins
Membrane fragments were obtained from Escherichia coli cells carrying the recombinant plasmid for the engineered FoF1 and were ... HPr kinase/phosphorylase (HprK/P), the bifunctional sensor enzyme for catabolite repression, phosphorylates HPr, a ... To effectively dephosphorylate P-Ser-HPr when glucose is exhausted, the pyrophosphate concentration in the cells is lowered to ... These results established that the c10-14 and 3 3ab2 complexes are mechanical units of the membrane-embedded enzyme involved in ...http://www.antievolution.org/cgi-bin/ikonboard/ikonboard.cgi?s=50aab1e243fb46f7
The metabolism beyond programmed cell death in yeast.
A cell's reaction to any change in the endogenous or exogenous conditions often involves a complex response that eventually ... either leads to cell adaptation and survival or to the initiation and execution of (programmed) cell death. The molecular deci ... Cells lacking malic enzyme gene (MAE1) showed an enormously increased flux from glucose-6-phophate into the PPP when grown on ... Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expressionMol. Cell. ...http://www.biomedsearch.com/nih/metabolism-beyond-programmed-cell-death/22480867.html
CandidatusLiberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype |...
... in many of enzymes. In addition, zinc is involved in the maintenance of membrane integrity and protection of cell structural ... such as ADP-glucose pyrophosphorylase (AGPase)) and repression of transcripts related to starch breakdown [6, 16, 17, 19]. ... we found an induction of transcripts encoding key enzymes involved in starch biosynthesis and repression of those related to ... Plant Cell. 2006, 18: 2710-2732. 10.1105/tpc.105.039404.PubMed CentralView ArticlePubMedGoogle Scholar. ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-247
Frontiers | Astragaloside IV Inhibits Triglyceride Accumulation in Insulin-Resistant HepG2 Cells via AMPK-Induced SREBP-1c...
We evaluated glucose, triglyceride (TG), and non-esterified fatty acid (NEFA) production, as well as SREBP-1c transcription, ... We evaluated glucose, triglyceride (TG), and non-esterified fatty acid (NEFA) production, as well as SREBP-1c transcription, ... AST also inhibited translocation of SREBP-1c into the nucleus of insulin resistant HepG2 cells by inducing phosphorylation of ... Results: We found that AST attenuated insulin resistance and lipid accumulation in HepG2 cells. As an AMPK activator, AST ...https://www.frontiersin.org/articles/10.3389/fphar.2018.00345/full
Frontiers | COX-Independent Mechanisms of Cancer Chemoprevention by Anti-Inflammatory Drugs | Oncology
Experimental studies in human cancer cell lines and rodent models of carcinogenesis support these observations by providing ... Experimental studies in human cancer cell lines and rodent models of carcinogenesis support these observations by providing ... 2 and 9 are the principal enzymes involved in degrading type IV collagen of the basement membrane allowing endothelial cells to ... As a consequence, typical features of ESR can be observed in celecoxib-treated cells. These include global repression of ...https://www.frontiersin.org/articles/10.3389/fonc.2013.00181/full
Hepatic glucose sensing is required to preserve β cell glucose competence. J Clin Invest 2013;123:1662-1676pmid:23549084. ... cell membranes (35,36); are more likely to activate additional BA-sensitive signaling pathways such as protein kinase C, ... The enzyme 12α-hydroxylase (CYP8B1) is responsible for the conversion of CDCA into CA. GCA, glycocholic acid; GCDCA, ... such as a release of FXR-mediated repression of Cyp7a1 and subsequent increase in BA synthesis (41). Finally, evidence now ...http://diabetes.diabetesjournals.org/content/62/12/4184
Human Insulin Resistance Is Associated With Increased Plasma Levels of 12α-Hydroxylated Bile Acids | Diabetes
Hepatic glucose sensing is required to preserve β cell glucose competence. J Clin Invest 2013;123:1662-1676pmid:23549084. ... cell membranes (35,36); are more likely to activate additional BA-sensitive signaling pathways such as protein kinase C, ... The enzyme 12α-hydroxylase (CYP8B1) is responsible for the conversion of CDCA into CA. GCA, glycocholic acid; GCDCA, ... such as a release of FXR-mediated repression of Cyp7a1 and subsequent increase in BA synthesis (41). Finally, evidence now ...http://diabetes.diabetesjournals.org/content/62/12/4184.full
Peroxisome Proliferator-Activated Receptor and Retinoic X Receptor in Alcoholic Liver Disease
... thus increasing oxidative stress and leading to membrane-lipids oxidation , furthermore oxidative stress depletes the cell ... regulates glucose metabolism and insulin sensitivity," Proceedings of the National Academy of Sciences of the United States of ... composed at least by six genes encode for microsomal enzymes . GST enzymes of alpha, mu, and pi families account for the ... repression of glycolytic genes in response to fasting and pyruvate . Knockdown of SIRT1 in liver causes mild hypoglycemia, ...https://www.hindawi.com/journals/ppar/2009/748174/
Induced stem cells - Wikipedia
... of these teratoma-initiating cells is associated with failed repression of Nanog as well as a propensity for increased glucose ... Teratoma formation by pluripotent stem cells may be caused by low activity of PTEN enzyme, reported to promote the survival of ... A common feature of pluripotent stem cells is the specific nature of protein glycosylation of their outer membrane. That ... human iPS cell-derived myeloid cell lines as unlimited cell source for dendritic cell-like antigen-presenting cells". Gene ...https://en.wikipedia.org/wiki/Induced_stem_cells
Protocols and Video Articles Authored by Scott E. Gold (Translated to Korean)
Cell wall degrading enzymes (CWDEs) in fungi are often subject to carbon catabolite repression at the transcriptional level ... In this yeast, Snf1p is required to release glucose repression when this sugar is depleted from the growth medium. We have ... is randomly sequestered within double-membrane bound vesicles termed autophagosomes and targeted for degradation. Initially ... The repression of a subset of genes in filamentous cells may be critical for programming virulence. To identify these filament ...https://www.jove.com/author/Scott+E._Gold?language=Korean
Strand-specific transcriptomes of Enterohemorrhagic Escherichia coli in response to interactions with ground beef microbiota:...
Genes implicated in cell wall/membrane biogenesis. The murF gene encoding the MurF amide ligase enzyme was repressed in O26 ... and is decreased in glucose minimal media enriched with amino acids . The repression of genes involved in amino acid ... Phage display-derived inhibitor of the essential cell wall biosynthesis enzyme MurF. BMC Biochem. 2008;9:33.View ArticlePubMed ... Utility of muropeptide ligase for identification of inhibitors of the cell wall biosynthesis enzyme MurF. Antimicrob Agents ...https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3957-2
Involvement of Physical Parameters in Medium Improvement for Tannase Production by Aspergillus niger FETL FT3 in Submerged...
The cell-free culture filtrate was then filtered again using a membrane filter with a pore size of 0.22 μm (Millipore) and was ... the decline in enzyme production may be due to gallic acid production as the present enzyme shows strict end-product repression ... a rapid breakdown of tannin allows cell multiplication by the assimilation of the degradation products , presumably glucose ... Enzyme activity as expressed in U per mL and one unit of tannase was defined as the amount of enzyme required to release 1.0 μ ...https://www.hindawi.com/journals/btri/2011/897931/
Targeting Cancer Metabolism | Clinical Cancer Research
Targeting glucose metabolism may be a selective way to kill cancer cells. Several glycolytic enzymes are required to maintain a ... AMPK and GCN2-ATF4 signal the repression of mitochondria in colon cancer cells. Biochem J 2012;444:249-59. ... act on mitochondrial membranes and/or on the permeability transition pore complex to induce apoptosis. Mitochondrial membrane ... Glucose-independent glutamine metabolism via TCA cycling for proliferation and survival in β cells. Cell Metab 2012;15:110-21. ...http://clincancerres.aacrjournals.org/content/18/20/5537.full
82) demonstrated the restoration effect of GLP-128-36 on β-cell mass and the improvement of glucose disposal. In a HFD-fed ... are cell-penetrating peptides that are transported through the plasma membrane without a receptor being involved (32, 61). ... including cardiac protection and the repression of glucose production, however, have been recognized in the cardiovascular ... represses the hepatic gluconeogenic enzymes, including phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase ( ...http://ajpendo.physiology.org/content/311/3/E620
Role of Peroxisome Proliferator-Activated Receptor and B-Cell Lymphoma-6 in Regulation of Genes Involved in Metastasis and...
... repression of pro-inflammatory and pro-migratory genes via BCL-6 influenced their ability to invade a basement membrane, ... The matrix metalloproteinases are a family of zinc-dependent proteolytic enzymes that degrade extracellular matrix (ECM) ... and cultured in high-glucose DMEM containing 10% FBS and 1% penicillin/streptomycin in a humidified atmosphere at 37°C ... increased cell migration in both cell lines compared with control cells. GW501516 treatment of BCL-6 repressed cells did not ...https://www.hindawi.com/journals/ppar/2013/121956/
Glucose sensing through the Hxk2-dependent signalling pathway | Biochemical Society Transactions
Once inside the cell, glucose activates another pathway involved in the repression of genes not needed during growth on glucose ... Nutrient Sensing through the Plasma Membrane of Eukaryotic Cell. Glucose sensing through the Hxk2-dependent signalling pathway ... occur at the level of mRNA transcription by the repression or activation of the transcription of many genes that encode enzymes ... proteins are necessary to generate the glucose repression signal. Thus, in hxk2 mutants repression of several genes by glucose ...http://www.biochemsoctrans.org/content/33/1/265
Cross‐Talk Between Hypoxia and the Tumour via Exosomes | IntechOpen
The post‐isolation membrane dye labelling is quick (∼1 h), whereas the transfection of cells requires additional time (∼2 weeks ... Thus, cells cultured under hypoxia or constitutive HIF‐1α expression promoted EMT, whereas the repression of TWIST expression ... 4 - Rice, G.E., et al., The effect of glucose on the release and bioactivity of exosomes from first trimester trophoblast cells ... like 2 and 3 enzymes, which promote tumour metastasis by mediating cells to matrix adhesion and stabilising SNAI1 activity to ...https://www.intechopen.com/books/hypoxia-and-human-diseases/cross-talk-between-hypoxia-and-the-tumour-via-exosomes/