Autotaxin through lysophosphatidic acid stimulates polarization, motility, and transendothelial migration of naive T cells. -...
In this article, we show that HA130, an inhibitor of the enzymatic activity of ATX, slows T cell migration across lymph node ... T cells possess Mn(+2)-activatable receptors for ATX, which are localized at the leading edge of polarized cells. ATX must bind ... Ex vivo, ATX plus LPC or LPA itself induces the polarization of mouse naive T cells and stimulates their motility on an ICAM-1 ... Under physiologic shear conditions in a flow chamber, LPA or ATX/LPC strongly enhances TEM of integrin-arrested T cells across ...https://www.semanticscholar.org/paper/Autotaxin-through-lysophosphatidic-acid-stimulates-Zhang-Chen/0ab0be8d7a0d3b7a00ef1b8992e38d97bb5a76d9
GANP® Mice for High Affinity Antibodies
Germinal center-associated nuclear protein contributes to affinity maturation of B cell antigen receptor in T cell-dependent ... It can be up-regulated in germinal center (GC) B cells upon immunization with T-cell dependent antigen. GANP carries two ... the acronym for Germinal Center Associated Nuclear Protein (GANP), is a recently discovered protein, over-expressed in B-cells ... To submit an antigen, mail it together with a completed hard copy of Antigen Submission Antigen Submission Form ...https://www.genscript.com/GANP-Transgenic-Mice.html
Toll-like receptor 9 antagonizes antibody affinity maturation The presentation of antigen by germinal-center B cells to ... T cell flow cytometry - analyzing antigen-specific T cells extra- and intracellularly 27 June 2018. - 28 June 2018. ... Pierce and colleagues show that TLR9 signaling in B cells antagonizes B cell-mediated antigen presentation, which leads to the ... The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers ...https://www.nature.com/ni/?error=cookies_not_supported&code=2ec63d9d-71d3-4d3a-8fb5-9a89c6af4004
Affinity Biologicals Incorporated Company Profile
Selective expansion of high-affinity antigen-specific B cells in germinal centers (GCs) is a key event in antibody affinity ... Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction. ... maturation. GC B cells with improved affinity can either continue affinity-d... ...https://www.bioportfolio.com/corporate/company/17570/Affinity-Biologicals-Incorporated.html
Affinity BioReagents, Inc Company Profile
Selective expansion of high-affinity antigen-specific B cells in germinal centers (GCs) is a key event in antibody affinity ... Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction. ... maturation. GC B cells with improved affinity can either continue affinity-d... ... This is a multi-center, randomized controlled trial designed to evaluate the use of fresh hypothermically stored human amniotic ...https://www.bioportfolio.com/corporate/company/21954/Affinity-BioReagents-Inc.html
Newest Articles | JEM
... germinal center flexibility permits nonspecific B cells to achieve de novo antigen recognition and antibody affinity maturation ... Left or right? Directions to stem cell engraftment In this issue of JEM, Wu et al. use genetic barcoding of macaque ... Stochasticity enables BCR-independent germinal center initiation and antibody affinity maturation Whether Ig engagement by ... Cilia loss sensitizes cells to transformation by activating the mevalonate pathway Cilia loss is common in cancer, and its ...http://jem.rupress.org/latest?page=6&variant=full-text.pdf
Short Communication on: Study of Avidity of Antigen-Specific Antibody as a Means of Understanding Development of Long-Term...
Study of Avidity of Antigen-Specific Antibody as a Means of Understanding Development of Long-Term Immunological Memory after ... of an effective memory response by interacting with B cells in the germinal center inducing antigen specific B cell maturation ... Upon exposure to an antigen, memory B cells can be generated in the germinal centre of the secondary lymphoid organs and can ... 2006) High affinity germinal center B cells are actively selected into the plasma cell compartment. J Exp Med203:2419-2424. ...https://www.omicsonline.org/open-access/short-communication-on-study-of-avidity-of-antigenspecific-antibody-as-a-means-of-understanding-development-of-longterm-immunologi-2157-7560-1000363.php?aid=91533
CD40 Ligand and Autoantigen Are Involved in the Pathogenesis of Low-Grade B-Cell Lymphomas of Mucosa-Associated Lymphoid Tissue
... that antigen and CD40 Ligand act during cognate T/B cell interaction and are crucial for germinal center B-cell maturation ... T cells may drive the evolution of low-grade MALT-type lymphomas either directly or by paracrine mechanisms and that antigen ... Low-grade MALT-type lymphomas are malignancies of mucosal marginal-zone B cells and preceded by reactive inflammatory lymphoid ... Different VH families were used and more than 69% of tumor immunoglobulins bound different mucosal antigens. CD40L expression ...https://www.hindawi.com/journals/jir/1998/018679/abs/
γδ T-cell help in responses to pathogens and in the development of systemic autoimmunity | Springer for Research & Development
Mice rendered deficient in αβ T-cells by single-gene knockout mutation show enhanced levels of autoantibody formation and even ... Pulendran B, Smith KG, Nossal GJ: Soluble antigen can impede affinity maturation and the germinal center reaction but enhance ... Bowen MB, Butch AW, Parvin CA, Levine A, Nahm MH: Germinal center T-cells are distinct helper-inducer T-cells. Human Immunol ... Effect of thymus cell injections on germinal center formation in lymphoid tissues of nude (thymusless) mice. Cell Immunol 1974 ...https://rd.springer.com/article/10.1007/BF02786392
Age, microbiota, and T cells shape diverse individual IgA repertoires in the intestine | JEM
... the one hand stimulate the maturation of GALTs and germinal centers and on the other hand provide a complex array of antigens. ... IgA-producing plasma cells originate from germinal centers that are induced by B-cell receptor engagement in humans. ... Moreover, SHM was strictly dependent on T cells and the presence of RORγt. Besides T cell-dependent germinal center reactions, ... Cell isolation, flow cytometry, and cell sorting.. For isolation of intestinal lamina propria cells, gut content and PPs were ...http://jem.rupress.org/content/209/2/365.long
CD301b+ dendritic cells suppress T follicular helper cells and antibody responses to protein antigens | eLife
CD301b dendritic cells potently suppress antibody responses by limiting the activity of follicular helper T cells and germinal ... Persistent antigen and germinal center B cells sustain T follicular helper cell responses and phenotype * D Baumjohann ... where B cells undergo affinity maturation, selection and proliferation. Follicular helper T cells (Tfh) are a subset of CD4+ T ... PD-1 regulates germinal center B cell survival and the formation and affinity of long-lived plasma cells * KL Good-Jacobson ...https://elifesciences.org/articles/17979
Chimeric camel/human heavy-chain antibodies protect against MERS-CoV infection | Science Advances
After immunization, antigen-stimulated B cells undergo affinity maturation in germinal centers of secondary lymphoid organs, ... The thawed cell suspension was quickly transferred to 40 ml of ice-cold RPMI 1640 (Lonza) medium. Cells were counted, and 107 ... Antigen-selected, immunoglobulin-secreting cells persist in human spleen and bone marrow. Blood 103, 3805-3812 (2004).. ... Targeting and tracing antigens in live cells with fluorescent nanobodies. Nat. Methods 3, 887-889 (2006).. ...http://advances.sciencemag.org/content/4/8/eaas9667.full
Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation | Garvan...
... noncognate B cells relayed antigen opsonized by newly produced antibodies from the subcapsular region to the germinal center, ... noncognate B cells relayed antigen opsonized by newly produced antibodies from the subcapsular region to the germinal center, ... Thus, we characterize SCS macrophages as specialized antigen-presenting cells functioning at the apex of an antigen transport ... Thus, we characterize SCS macrophages as specialized antigen-presenting cells functioning at the apex of an antigen transport ...https://www.garvan.org.au/research/publications/10276
Monoclonal IgG antibodies generated from joint-derived B cells of RA patients have a strong bias toward citrullinated...
Maturation of the immune response in germinal centers. Cell. 67:1121-1129. doi:10.1016/0092-8674(91)90289-B. ... affinity maturation is primarily dependent on the presentation of antigens to T cells and takes place in germinal centers (Liu ... Imaging of germinal center selection events during affinity maturation. Science. 315:528-531. doi:10.1126/science.1136736. ... Single B cell sorting. Cryopreserved synovial mononuclear cells were thawed, and the cell suspensions were stained with anti- ...http://jem.rupress.org/content/early/2013/02/19/jem.20121486
Selection and maturation of B cells into plasma cells producing high-affinity antibodies occur in germinal centers (GC). GCs ... The androgen receptor controls expression of the cancerassociated sTn antigen and cell adhesion through induction of ST6GalNAc1 ... BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-β receptor type I ALK5 ... Autologous bone marrow Th cells can support multiple myeloma cell proliferation in vitro and in xenografted mice ...https://www.duo.uio.no/handle/10852/233/discover?rpp=100&sort_by=dc.date.issued_dt&order=DESC
Follicular Helper T Cells in Immunity and Autoimmunity | Frontiers Research Topic
... are professional B cell helper cells in germinal centers (GCs), lymphoid structures where B cell maturation into high affinity ... plasma cells and memory B cells takes place. Upon antigen encounter, a fraction of T cells upregulates chemokine receptor CXCR5 ... These CXCR5-expressing T cells upregulate Bcl-6, acquire the full differentiation program of the Tfh cell lineage, and help B ... that allows their locali-zation inside the B cell follicles where together with dendritic cells and B cells initiate the GC ...https://www.frontiersin.org/research-topics/7025/follicular-helper-t-cells-in-immunity-and-autoimmunity
B cell - Wikipedia
... have antibodies with a higher affinity towards their target antigen due to affinity maturation in the germinal center (GC) and ... Plasma cells typically result from the germinal center reaction from T cell-dependent activation of B cells, however they can ... Antigens that activate B cells with the help of T-cell are known as T cell-dependent (TD) antigens and include foreign proteins ... At the SLO, B cell activation begins when the B cell binds to an antigen via its BCR. Of the three B cell subsets, FO B cells ...https://en.wikipedia.org/wiki/B_cell
Through the miracle of genetic engineering, you have generated ... - TechyLib
germinal center. site of B cell proliferation and affinity maturation during T dependent B cell response to. antigen.. ... Cell in germinal center that displays antigen on its surface and helps to select high. affinity B cells during affinity ... Can be used to determine the number of cells in a cell suspension that express a particular antigen.. b). Sensitive method used ... Dimeric (2 antigen binding sites/molecule).. b). Tetrameric (4 antigen binding sites/molecule).. c). Dec. americ (10 antigen ...https://www.techylib.com/el/view/taxmanstrong/through_the_miracle_of_genetic_engineering_you_have_generated
Welcome to CDC stacks | A Simple Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity Enables...
Our study describes a simple flow-cytometric method that enumerates virus-specific germinal center (GC) B cells as well as ... Limitations in methods for characterizing antigen-specific B cells have impeded progress in characterizing the quality of ... A Simple Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity Enables Characterization of Affinity Maturation ... a simple flow cytometry-based approach that measures both the number and affinity of IAV-binding germinal center B cells ...https://stacks.cdc.gov/view/cdc/32910
Composition of the CD95 DISC in freshly isolated and cu | Open-i
A) CD95 or (B) FADD was immunoprecipitated (IP) from 107 freshly isolated GC B cells ... Composition of the CD95 DISC in freshly isolated and cultured GC B cells. ( ... Affinity maturation of the B cell response to antigen (Ag) takes place in the germinal centers (GCs) of secondary follicles. ... Affinity maturation of the B cell response to antigen (Ag) takes place in the germinal centers (GCs) of secondary follicles. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2195905_JEM001318.f5&req=4
Role of framework mutations and antibody flexibility in the evolution of broadly neutralizing antibodies | eLife
Activated B cells can seed structures called germinal centers (GCs) in lymph nodes where affinity maturation occurs. During ... The probability that a B cell successfully internalizes an antigen i that it encounters also depends on the antigen ... 2] If the number of B cells in the GC exceeds 1,536 cells; this is a proxy for the antigen being consumed by the B cells 3] ... In the GC, B cells interact with antigen displayed on FDCs. B cells with receptors that bind more strongly to the antigen will ...https://elifesciences.org/articles/33038
This Week in Science | Science
This process takes place in the germinal center, where B cells are also thought to compete vigorously for available antigen. ... male germline stem cells orient the mitotic spindle to generate a daughter stem cell and a cell destined for germ cell ... B cells become effective factories for antibody production only after they have gone through a series of maturation steps that ... of germinal center B cells is more consistent with a competition for the attention of helper T cells than for scarce antigen. ...http://science.sciencemag.org/content/315/5811/twis
Frontiers | Computational Model Reveals Limited Correlation between Germinal Center B-Cell Subclone Abundancy and Affinity:...
The expanded subclones are expected to have BCR affinities for the Ag higher than the affinities of the naive B cells in the ... Given the nature of affinity maturation one would expect that abundant subclones are of high affinity but since repertoire ... The expanded subclones are expected to have BCR affinities for the Ag higher than the affinities of the naive B cells in the ... One challenge is the selection of the Ag-specific B cells from the measured repertoire for downstream analyses. A general ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00221/full
Germinal center - Wikipedia
... cells in the presence of follicular dendritic cells (FDCs). Germinal centers are an important part of the B cell humoral immune ... Within lymph nodes, mature peripheral B cells known as follicular (Fo) B cells acquire antigen from follicular dendritic cells ... Meyer-Hermann ME (2002). "A Mathematical Model for the Germinal Centre Morphology and Affinity Maturation". J. Theor. Biol. 216 ... Interactions with T cells are also believed to prevent the generation of autoreactive germinal center B cells. At some unclear ...https://en.wikipedia.org/wiki/Germinal_center
Avidity of anti-malarial antibodies inversely related to transmission intensity at three sites in Uganda | Malaria Journal |...
Affinity maturation is acquired through somatic hypermutation during germinal centre reactions, with B cell affinity to antigen ... with short lived plasma cells (SLPC) emerging from germinal centres initially, followed by memory B cells (MBC) and then, weeks ... In general, repeated exposure to an antigen results in germinal centre reactions which lead to affinity maturation and ... Severe malaria infections impair germinal center responses by inhibiting T follicular helper cell differentiation. Cell Rep. ...https://malariajournal.biomedcentral.com/articles/10.1186/s12936-017-1721-3