IJMS | Free Full-Text | The Clinical Utilization of Circulating Cell Free DNA (CCFDNA) in Blood of Cancer Patients | HTML
Detecting circulating DNA in cancer patients may help develop a DNA profile for early stage diagnosis in malignancies. The ... Qualitative and quantitative testing of circulating cell free DNA (CCFDNA) can be applied for the management of malignant and ... Cell-free tumor DNA in blood plasma as a marker for circulating tumor cells in prostate cancer. Clin. Cancer Res 2009, 15, 1032 ... Aberrant CpG island hypermethylation rarely occur in non-neoplastic and normally differentiated cells. Therefore, the DNA ...http://mdpi.com/1422-0067/14/9/18925/htm
Circulating Tumor Cells from Patients with Advanced Prostate and Breast Cancer Display Both Epithelial and Mesenchymal Markers ...
Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic ... Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature 2009;457:603-7. ... Circulating Tumor Cells from Patients with Advanced Prostate and Breast Cancer Display Both Epithelial and Mesenchymal Markers ... Circulating Tumor Cells from Patients with Advanced Prostate and Breast Cancer Display Both Epithelial and Mesenchymal Markers ...http://mcr.aacrjournals.org/content/9/8/997
Neutrophils Suppress Intraluminal NK Cell-Mediated Tumor Cell Clearance and Enhance Extravasation of Disseminated Carcinoma...
Tumor-elicited polymorphonuclear cells, in contrast to "normal" circulating polymorphonuclear cells, stimulate invasive and ... In the present study, we demonstrate that the systemic effects initiated by neoplastic cells residing in the primary tumor ... elevated levels of circulating neutrophils represent a marker of poor prognosis in patients with cancer (29, 30). ... injected tumor cells could result from two alternative mechanisms: (i) tumor cells disseminated by the 4T1 primary tumors ...http://cancerdiscovery.aacrjournals.org/content/6/6/630
An immunomagnetic epithelial tumor cell enrichment model for minimal residual disease detection of cytokeratin 8+ malignancies.
Immunocytochemical detection of isolated tumor cells in peripheral blood and bone marrow is currently the most established ... Neoplastic Cells, Circulating / metabolism. Sensitivity and Specificity. Time Factors. Tumor Markers, Biological / analysis. ... Cell Line, Tumor. Cell Survival. Cryopreservation. Epithelial Cells / cytology*, pathology. Flow Cytometry / methods*. Humans. ... Labelled cells were enriched by the use of magnetic columns. The eluted cytokeratin 8+ tumor cells were detected by flow ...http://www.biomedsearch.com/nih/immunomagnetic-epithelial-tumor-cell-enrichment/16178280.html
Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood...
Melanoma PatientCirculate Tumor CellSW620 CellCD45 DepletionPositive Enrichment. Background. In a variety of neoplastic ... Circulating tumor cell as a diagnostic marker in primary lung cancer. Clin Cancer Res. 2009, 15: 6980-6. 10.1158/1078-0432.CCR- ... Cells were resuspended in this buffer at a concentration of 1 × 108 cells/mL. The enrichment of tumor cells by CD45 depletion ... Detection of circulating tumor cells in peripheral blood of patients with renal cell carcinoma correlates with prognosis. ...https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-9-70
Loss of Heterozygosity at Tumor Suppressor Genes Detectable on Fractionated Circulating Cell-Free Tumor DNA as Indicator of...
Apart from killing of neoplastic cells, necrosis may also cause death of stromal cells surrounding the primary tumor. Moreover ... LOH at the markers D3S1605, D10S1765, D12S1725, D13S218, and D17S855 significantly correlated with tumor stage, tumor size, and ... Tumor cells that circulate in the blood can also contribute to the release of DNA (9). However, circulating tumor DNA is ... Comparative analysis of the genetics of circulating tumor cells and cell-free DNA in cancer patients are now feasible and they ...http://clincancerres.aacrjournals.org/content/18/20/5719
Splenic marginal zone lymphoma - Wikipedia
The neoplastic cells circulating in the peripheral blood are termed villous lymphocytes due to their characteristic appearance ... "Splenic B cell lymphoma with circulating villous lymphocytes: differential diagnosis of B cell leukaemias with large spleens". ... The relevant markers that define the immunophenotype for SMZL are shown in the adjacent table. The lack of CD5 expression is ... World Health Organization Classification of Tumors. 3. Lyon: IARC Press. ISBN 92-832-2411-6. CS1 maint: Multiple names: authors ...https://en.wikipedia.org/wiki/Splenic_marginal_zone_lymphoma
Epithelial-Mesenchymal Transition and Stem Cell Markers in Patients with HER2-Positive Metastatic Breast Cancer | Molecular...
Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic ... In many respects, cells that have undergone EMT behave similar to stem cells isolated from normal or neoplastic cell ... Clinical value of circulating endothelial cells and circulating tumor cells in metastatic breast cancer patients treated first ... Circulating tumor cells lacking cytokeratin in breast cancer: the importance of being mesenchymal. J Cell Mol Med 2011;15:1066- ...http://mct.aacrjournals.org/content/11/11/2526?rss=1
Cellular Image Analysis and Imaging by Flow Cytometry
Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors. Int J ... this approach depends largely on the availability of fluorescently-labeled markers to discriminate normal from neoplastic cells ... Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant ... In this case, a combination of enrichment using a marker specific to epithelial cells (e.g., the epithelial cell adhesion ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2034394/?lang=en-ca
Prognostic Value of Preoperative Serum Cell-Free Circulating DNA in Men with Prostate Cancer Undergoing Radical Prostatectomy |...
Only a low serum tumor marker concentration correlated with the amount of cell-free circulating DNA (14). In our study, we were ... from tumor cells. Evidence for this is based on cell-free circulating DNA having similar genetic and epigenetic properties as ... Stroun M, Anker P, Maurice P, Lyautey J, Lederrey C, Beljanski M. Neoplastic characteristics of the DNA found in the plasma of ... Association of cell-free circulating DNA levels with disease progression. The mean concentration of cell-free circulating DNA ...http://clincancerres.aacrjournals.org/content/13/18/5361
Isolation of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Using a Novel Cell Separation Strategy |...
... a well-known marker for HCC, is expressed in the cytoplasm of HCC cells. AFP mRNA in the peripheral blood detected by reverse ... Expression of Ep-CAM in normal, regenerating, metaplastic, and neoplastic liver. J Pathol 1999;188:201-6. ... In the samples spiked with 10 cells, none had less than 5 tumor cells detected. No tumor cells were detected in any samples ... Isolation of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Using a Novel Cell Separation Strategy. Wen Xu, ...http://clincancerres.aacrjournals.org/content/17/11/3783
The Prognostic Significance of Tumor-associated Antigen 22-1-1 Expression in Adenocarcinoma of the Uterine Cervix | Clinical...
Duk J. M., Aalders J. G., Fleuren G. J., Krans M., de Bruijn H. W. A. Tumor markers CA 125, squamous cell carcinoma antigen, ... and CA 19-9 in normal and neoplastic glandular cells of the uterine cervix. Cancer (Phila.), 62: 2580-2588, 1988. ... Schwartz P. E., Chambers S. K., Chambers J. T., Gutmann J., Katopodis N., Foemmel R. Circulating tumor markers in the ... The 22-1-1 Ag existed both in the cytoplasm and on the membrane of cancer cells. More than 50% of reactive tumor cells were ...http://clincancerres.aacrjournals.org/content/5/6/1449
Gene expression analysis of bone metastasis and circulating tumor cells from metastatic castrate-resistant prostate cancer...
... cells were spiked into normal blood to assess cell recovery rate. RNA extracted from recovered PC3 cells was amplified using an ... Conversely, circulating tumor cells (CTCs), which are likely to stem from bone metastases, can be isolated from blood. The ... through in vitro transcription may be useful as a method to detect gene expression in small numbers of CTCs and tumor cells ... We found that in 21 out of 28 comparisons, the presence or absence of detectable gene expression in CTCs and PCa cells ...https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-016-0829-5
Diabetes and Cancer | Diabetes Care
... the effects of insulin receptor activation on neoplastic cells may relate more to cell survival and mitogenesis than to ... insulin receptor may be a marker of breast cancer cell differentiation and identify cells with a potentially less aggressive ... Glucose deprivation contributes to the development of KRAS pathway mutations in tumor cells. Science 2009; 325: 1555-1559. ... Increased circulating insulin has a number of indirect effects including a reduction in the hepatic synthesis and blood levels ...http://care.diabetesjournals.org/content/33/7/1674.full
Rasheed, Z. A.<...
Circulating tumor cells expressing markers of tumor-initiating cells predict poor survival and cancer recurrence in patients ... Circulating Tumor Cell Phenotype Predicts Recurrence and Survival in Pancreatic Adenocarcinoma. Poruk, K. E., Valero, V., ... Neoplastic Stem Cells Medicine & Life Sciences Pancreatic Neoplasms Medicine & Life Sciences Adenocarcinoma Medicine & Life ... Ki-67 is required for maintenance of cancer stem cells but not cell proliferation. Cidado, J. , Wong, H. Y. , Marc Rosen, D. , ...https://jhu.pure.elsevier.com/en/persons/zeshaan-rasheed
Targeted drug delivery to mesothelioma cells using functionally selected internalizing human single-chain antibodies |...
Mesothelioma thus shares cell surface markers with other tumors; therefore, treatments developed for other neoplastic diseases ... Immunoliposomes can be constructed to have a long circulating half-life and to be nonimmunogenic (46). As such, immunoliposomes ... Besides serving as markers for malignant cells, tumor cell surface molecules are valuable targets for therapy due to their ... Binding to Mesothelioma Cells In situ. To determine if scFvs selected on mesothelioma cell lines recognize tumor cells in situ ...http://mct.aacrjournals.org/content/7/3/569.long
Endothelial progenitor cells in non-Hodgkin's lymphoma | Haematologica
Interestingly, circulating EPC can be either of non-malignant cell origin or may originate from the same neoplastic clone. For ... angiogenic factors can be produced by the tumor cells themselves but also by surrounding hematopoietic cells in the tumor ... 4 These cells expressed several markers in common with endothelial cells such as CD34, CD31, VEGFR2 (KDR), and Tie2.4 Further ... 2006) Neoplastic circulating endothelial cells in multiple myeloma with 13q14 deletion. Blood 107:2531-5. ...http://www.haematologica.org/content/92/4/433
cell cycle*retinoblastoma genes*dna mutational analysis*cultured tumor cells*biological tumor markers*proto oncogene proteins* ... neoplastic gene expression regulation*cell cycle proteins*genetic predisposition to disease*cyclin dependent kinases* ... Validation studies of circulating methylation biomarkers. Stephen Meltzer; Fiscal Year: 2005. *P16 GENE FAMILY IN HEALTH AND ... Exp Cell Res 264:42-55. 2001. ..It is now believed that loss of p16 is an early and often critical event in tumor progression. ...https://www.labome.org/topics/physical/natural/biological/genetic/inheritance/recessive/tumor/p16-genes-20254.html
Circulating tumour cells in the central and the peripheral venous compartment in patients with metastatic breast cancer.
The enumeration of circulating tumour cells (CTC) has prognostic significance in patients with metastatic breast cancer (MBC) ... 2009Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of ... Neoplastic Cells, Circulating*. Predictive Value of Tests. Prognosis. Veins*. Comments/Corrections. From MEDLINE®/PubMed®, a ... Year: 2008Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in ...http://www.biomedsearch.com/nih/Circulating-tumour-cells-in-central/21468046.html
Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays -...
... malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans ... as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or ... The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other ... Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, ...http://www.patentgenius.com/patent/6939675.html
The Circulating Dendritic Cell Compartment in Patients with Chronic Lymphocytic Leukemia Is Severely Defective and Unable to...
Neoplastic CLL cells are known to produce and release many cytokines, including IL-1, IL-6, IL-8, IL-10, tumor necrosis factor ... NK cell, and T- and B-cell markers (Lin−). HLA-DR+/Lin− DCs represented on average 57% (range, 32-87%) of the purified ... Cells were then washed and stained with cell surface-conjugated mAbs as described. For intracellular staining, cells were then ... DCs play a pivotal role in the induction of T-cell immunity, including tumor-specific T-cell responses. Thus far, little ...http://cancerres.aacrjournals.org/content/63/15/4497
Hairy cell leukemia - Renal and Urology News
Patients often present with pancytopenia with very few circulating neoplastic hairy cells. Monocytopenia is a characteristic ... CB-839 Drug Promising for Clear Cell, Papillary Renal Cell Cancer. *Ceruloplasmin a Possible Marker of CKD in Sickle Cell ... Tumor cells are predominately found in the peripheral blood, bone marrow and spleen. One has to consider hairy cell leukemia in ... It is a clonal B-cell, derived from a mature memory B-cell. The leukemic hairy cell differs from the normal B-cell counterpart ...http://www.renalandurologynews.com/oncology/hairy-cell-leukemia/article/619385/
Hairy cell leukemia - ONA
Patients often present with pancytopenia with very few circulating neoplastic hairy cells. Monocytopenia is a characteristic ... Tumor cells are predominately found in the peripheral blood, bone marrow and spleen. One has to consider hairy cell leukemia in ... However, hairy cell leukemia has a distinct immunophenotype and CD103 is the most specific marker by flow cytometry. Annexin A1 ... It is a clonal B-cell, derived from a mature memory B-cell. The leukemic hairy cell differs from the normal B-cell counterpart ...https://www.oncologynurseadvisor.com/hematology/hairy-cell-leukemia/article/597265/
Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver...
... variations of rs894151 and rs12438080 in pretransplant plasma circulating DNA are promising prognostic markers for tumor ... Methods Oligonucleotide microarrays were used to evaluate the genetic characteristics of tumor DNA in 30 HBV-related HCC ... Genetic variations play an important role in tumor development and metastasis. ... using pretransplant plasma circulating DNA. Prognostic significance was assessed by Kaplan-Meier survival estimates and log- ...https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0026003
Frontiers | Professional antigen presenting cells in human herpesvirus 8 infection | Immunology
This could be why T cell responses to HHV-8 antigens are not very robust. Of these APC, only B cells support complete, lytic ... This could be why T cell responses to HHV-8 antigens are not very robust. Of these APC, only B cells support complete, lytic ... dendritic cells, monocytes/macrophages, and B lymphocytes, are critically important in the recognition of an invading pathogen ... and HHV-8 associated B cell cancers. In this review, we discuss the effects of HHV-8 infection on professional APC and their ...https://www.frontiersin.org/articles/10.3389/fimmu.2012.00427/full