"P38 Phoshporylates RB On SER567 By A Novel, Cell Cycle-Independent Mec" by Rachel Delston
The retinoblastoma protein: Rb) inhibits both cell division and apoptosis, but the mechanism by which Rb alternatively regulates these divergent outcomes remains poorly understood. Cyclin dependent kinases: Cdks) promote cell division by phosphorylating and reversibly inactivating Rb by a hierarchical series of phosphorylation events and sequential conformational changes. The stress-regulated mitogen activated protein kinase: MAPK) p38 also phosphorylates Rb, but it does so in a cell cycle-independent manner that is associated with apoptosis rather than with cell division. Here, we show that ...https://openscholarship.wustl.edu/etd/86/
Cell Cycle Synchronization | Springer for Research & Development
This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Celhttps://rd.springer.com/book/10.1007/978-1-4939-6603-5
Publication : USDA ARS
Citation: Li, C. 2011. Specific cell cycle synchronization with butyrate and cell cycle analysis. In: Banfalvi, G., editor. Methods in Molecular Biology, Cell Cycle Synchronization: Methods and Protocols. New York NY: Humana Press. p. 125-136. Interpretive Summary: Cultured cells usually do not grow at the same pace. However, study of cell growth regulation requires cultured cells proliferating at the same pace (i.e.,synchronization). Butyrate can block cells cycle, therefore can stop cell at specific stage of cell cycle. In this study, we investigated the property of the ...https://www.ars.usda.gov/research/publications/publication/?seqNo115=246814
Frontiers | Pharmacodynamic Modeling of Cell Cycle Effects for Gemcitabine and Trabectedin Combinations in Pancreatic Cancer...
Combinations of gemcitabine and trabectedin exert modest synergistic cytotoxic effects on two pancreatic cancer cell lines. Here, systems pharmacodynamic (PD) models that integrate cellular response data and extend a prototype model framework were developed to characterize dynamic changes in cell cycle phase of cancer cell subpopulations in response to gemcitabine and trabectedin as single agents and in combination. Extensive experimental data were obtained for two pancreatic cancer cell lines (MiaPaCa-2 and BxPC-3), including cell proliferation rates over 0-120 h of drug exposure, and the fraction of ...https://www.frontiersin.org/articles/10.3389/fphar.2016.00421/full
Cell Cycle - QIAGEN
The cell cycle includes 4 main phases: Gap 1 (G1), DNA replication (S), Gap 2 (G2), and mitosis (M). Tight regulation of the transition between these phases halts cell cycle progression if a phase is not properly completed. For example, the G2-M DNA damage checkpoint ensures the fidelity of DNA replication, and arrests the cell cycle to allow time for replication error correction and DNA damage repair. Cell cycle progression is regulated by the cyclic rise and fall of kinase expression, and their interaction with, and action on, their cyclin targets. ...https://www.qiagen.com/cn/shop/genes-and-pathways/complete-biology-list/cell-cycle/
Expression of cell cycle regulatory proteins cyclin B1, cyclin E, and cdk2 during the first three cell cycles of...
The cell cycle is a highly regulated process driven by endogenous factors that have regulatory functions. Certain proteins such as cyclins and cyclin-dependent kinases (cdks) are needed to progress through the four phases of the cell cycle. Cell cycle regulatory proteins have been characterized in somatic cells and exhibit phase specific expression patterns. However, the changes in expression of these proteins have not been characterized in early cleavage stage mouse embryos. This study utilized indirect immunofluorescence microscopy to determine the expression pattern of cell cycle regulators cyclin B 1, ...http://cardinalscholar.bsu.edu/handle/handle/186406
Impact of the Cell Cycle Progression Test on Physician and Patient Treatment Selection for Localized Prostate Cancer. -...
PURPOSE The cell cycle progression test is a validated molecular assay that assesses prostate cancer specific disease progression and mortality risk when combined with clinicopathological parameters. We present the results from PROCEDE-1000, a large, prospective registry designed to evaluate the impact of the cell cycle progression test on shared treatment decision making for patients newly diagnosed with prostate cancer. MATERIALS AND METHODS Untreated patients with newly diagnosed prostate adenocarcinoma were enrolled in the study and the cell cycle progression test was ...https://www.semanticscholar.org/paper/Impact-of-the-Cell-Cycle-Progression-Test-on-Physi-Shore-Kella/4d014549429bc7300a04f2fa13d20da45f158394
Essay about Mitosis and Cell Cycle Study - 747 Words
Cell Growth and Reproduction Study Guide The Cell Cycle Study Guide Vocabulary - Cell Cycle, Mitosis, Cytokinesis 1. How did the G1 and G2 stages get theirhttp://www.studymode.com/essays/Mitosis-And-Cell-Cycle-Study-1261495.html
Sam68 exerts separable effects on cell cycle progression and apoptosis | BMC Cell Biology | Full Text
Despite abundant circumstantial clues to its cellular function, an understanding of the precise cellular role of Sam68 and the significance of its RNA binding ability has proved elusive. Among the processes that it appears to regulate are transcription, splicing, RNA export and translation. The possibility that Sam68 might play multiple roles in gene expression is supported by the present study in which we show that its effects on cell proliferation and death are functionally separable.. We found that Sam68 compromises cell proliferation without the need for specific RNA binding. At least ...https://bmccellbiol.biomedcentral.com/articles/10.1186/1471-2121-5-5
Wiley: Annual Plant Reviews, Volume 32, Cell Cycle Control and Plant Development - Dirk Inz
1. The growing family of plant cyclin-dependent kinases with multiple functions in cellular and developmental regulation. 2. The plant cyclins.. 3. CDK inhibitors.. 4. The UPS: an engine that drives the cell cycle.. 5. CDK phosphorylation.. 6. E2F-DP transcription factors.. 7. Function of the retinoblastoma-related protein in plants.. 8. Auxin fuels the cell cycle engine during lateral root initiation.. 9. Cell cycle control during leaf development.. 10. Physiological relevance and molecular control of the endocycle in plants.. 11. Insights into the endocycle from trichome development.. 12. ...http://www.wiley.com/WileyCDA/WileyTitle/productCd-0470994320.html
"Regulation of Zygotic Transcription and Cell Cycle Checkpoints in Earl" by Xiang Maomao Zhang
For many organisms, the first goal of embryogenesis is to accumulate a large cell population to accommodate gastrulation. To achieve this quickly, embryos employ specialized cell cycles called cleavages that consist of continuous rounds of DNA replication and division. Cell proliferation occurs rapidly because cleavage cycles lack the gap phases and cell cycle checkpoints found in canonical cell cycles. Further, the genetic materials required to sustain cleavage cycles are preloaded during oogenesis, aiding efficient cell cycle progression. After a constant, ...https://repository.upenn.edu/edissertations/1521/
Organ-specific cell division abnormalities caused by mutation in a general cell cycle regulator in C. elegans | Development
Cell proliferation is essential for many key processes that occur during development including organogenesis, tissue renewal and germline formation. (Bartkova et al., 1997; Clurman and Roberts, 1995; Pines, 1995; Sandhu and Slingerland, 2000). Therefore, the timing of cell division and differentiation must be precisely coordinated with signals that specify morphogenesis, patterning and growth in a temporal, positional and cell type-specific manner (reviewed by Vidwans and Su, 2001). This coordination is executed through regulating both positive and negative regulatory components of the basal cell cycle machinery.. The cell ...http://dev.biologists.org/content/129/9/2155
Regulation of the Chlamydomonas Cell Cycle by a Stable, Chromatin-Associated Retinoblastoma Tumor Suppressor Complex | Plant...
A fundamental principle of cell cycle control involves regulatory switches that integrate internal and external information and drive coherent and unidirectional cell cycle progression (Morgan, 2007). A key regulator of the G1-S transition is the retinoblastoma (RB) tumor suppressor pathway. RB is part of a cell cycle network involving cyclin-dependent kinases (CDKs) that phosphorylate RB-related proteins (also referred to as pocket proteins) to regulate the initiation of S-phase (Gutierrez et al., 2002; Burkhart and Sage, 2008). Pocket proteins repress the cell cycle by binding to the E2F ...http://www.plantcell.org/content/22/10/3331?ijkey=a1374ba04c7bcdc32c4984519f899e95206f31fc&keytype2=tf_ipsecsha
Targeted interactomics reveals a complex core cell cycle machinery in **Arabidopsis thaliana** - Institutional Repository...
Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Over 100 new candidate cell cycle proteins were ...https://repository.uantwerpen.be/link/irua/83428
The molecular mechanism regulating the timing of cell cycle exit during development of the mouse organ of Corti :: University...
During development of the cochlea, cells within the prospective prosensory domain undergo a wave of cell cycle exit, which defines the number of postmitotic sensory progenitors giving rise to the hair cells and supporting cells of the organ of Corti. Previous studies indicated that p27 (Kip1), a cyclin-dependent kinase inhibitor, is required for the timely cell cycle exit of sensory progenitors. However, how the pattern of p27 expression is established in development, and how the timing of cell cycle exit affects morphogenesis of the organ ...http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/158103/rec/2
"Targeting Cell Cycle Proteins in Breast Cancer Cells with siRNA by Usi" by Manoj Parmar, Hamidreza Montazeri Aliabadi et al.
The cell cycle proteins are key regulators of cell cycle progression whose de-regulation is one of the causes of breast cancer. RNA interference (RNAi) is an endogenous mechanism to regulate gene expression and it could serve as the basis of regulating aberrant proteins including cell cycle proteins. Since the delivery of small interfering RNA (siRNA) is a main barrier for implementation of RNAi therapy, we explored the potential of a non-viral delivery system, 2.0 kDa polyethylenimines substituted with linoleic acid and caprylic acid, for this purpose. Using a library of siRNAs against cell cycle proteins, we identified ...http://digitalcommons.chapman.edu/pharmacy_articles/142/
Interferon-λ1 induces G1 phase cell cycle arrest and apoptosis in gastric carcinoma cells in vitro
In recent years, increasing research has focused on the relationship between cytokines and tumorigenesis. It has been suggested that cytokines may be a new therapeutic option for tumors (16-20). In our experiments, we demonstrated that IFN-λ1 inhibited the growth of gastric carcinoma cells in a concentration-dependent manner. These data suggest that IFN-λ1 may be a potential antitumor agent for the treatment of gastric cancer.. Impaired apoptotic induction and dysregulated cell cycle progression are important factors in cancer development. Accordingly, inhibition of cell cycle regulation is particularly useful in the treatment of cancer. In our in vitro study, we demonstrated the ...https://www.spandidos-publications.com/or/32/1/199
Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication | BMC Cell ...
Tumor cells stably transfected with fluorescent proteins enable scientists to visualize many important aspects of cancer in real time at the single cell level. For example, transfected tumor cells have been visualized either through surgically created chronic-transparent windows or directly through the opened skin of living animals . This intravital imaging provides a powerful tool for observing cancer initiation and progression and evaluating the efficacy of candidate cancer drugs in vivo. On the other hand, assays using tumor cells grown in culture provide reliable information about cancer mechanisms, and are amenable to automated high-throughput screening [16-20]. Using a modified fluorescent indicator of cell cycle ...https://bmccellbiol.biomedcentral.com/articles/10.1186/1471-2121-12-2
KEGG PATHWAY: Cell cycle - Homo sapiens (human)
Mitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cyclin-dependent kinases (CDKs) are key regulatory enzymes, each consisting of a catalytic CDK subunit and an activating cyclin subunit. CDKs regulate the cell's progression through the phases of the cell cycle by modulating the activity of key substrates. Downstream targets of CDKs include transcription factor E2F and its regulator Rb. Precise activation and inactivation of CDKs at specific points in the cell cycle ...http://www.genome.jp/kegg-bin/show_pathway?133840406410261/hsa04110.args
'g2 phase' Protocols and Video...
Video articles in JoVE about 'g2 phase' include 'Cell Cycle Analysis in the C. Elegans Germline with the Thymidine Analog EdU', 'Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols', 'Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes', 'Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay', 'Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage', 'Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional ...https://www.jove.com/keyword/g2+phase
PLOS Biology: The Cell Cycle Timing of Centromeric Chromatin Assembly in Drosophila Meiosis Is Distinct from Mitosis Yet...
The centromeric histone CENP-A is incorporated at different cell cycle phases during somatic mitosis, meiosis I and meiosis II in Drosophila melanogaster.http://journals.plos.org/plosbiology/article/figure?id=10.1371/journal.pbio.1001460.g004
Cell Cycle Gene
What is Cell Cycle Gene? Definition of Cell Cycle Gene. Cell Cycle Gene FAQ. Learn more about Cell Cycle Gene. Cell Cycle Gene facts.http://www.cancerterms.net/define-Cell+Cycle+Gene/
E2-2 alteration influences cell cycle exit of progenito | Open-i
E2-2 alteration influences cell cycle exit of progenitors in vivo. (A)E2-2 overexpression increased cell cycle exit (EdU+Ki67-/EdU+) among the progenitor cellhttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4274746_1749-8104-9-23-5&req=4
"HIV-1 gp120 and morphine induced oxidative stress: role in cell cycle " by Thangaval Samikkannu, Deep Ranjith et al.
HIV infection and illicit drugs are known to induce oxidative stress and linked with severity of viral replication, disease progression, impaired cell cycle regulation and neurodegeneration. Studies have shown that morphine accelerates HIV infection and disease progression mediated by Reactive oxygen species (ROS). Oxidative stress impact redox balance and ROS production affect cell cycle regulation. However, the role of morphine in HIV associated acceleration of oxidative stress and its link to cell cycle regulation and neurodegeneration has not been elucidated. The aim of present study is to elucidate the mechanism of oxidative stress induced glutathione synthases (GSS), super oxide dismutase (SOD), and glutathione ...http://digitalcommons.fiu.edu/com_facpub/48/
Background Adenocarcinoma, the most common form of lung malignancy, is 1 - Drug Resistance to EGFR Inhibitors in Lung Cancer
0.05, C< 0.01 vs. the control group). Analysis ... Cell cycle analysis The cells were cultured in a six-well plate for 24 hours and then incubated with different ratios of CIK (10:1, 20:1, or 30:1) for 72 hours. The cells were then digested, resuspended, incubated with P-gp antibodies for 30?moments at 4C, and washed twice in PBS. As demonstrated in Table?1, CIK cells caused a significant dose-dependent build up of A549 cells in the G2/M phases, lightly in the G0/G1, and a decrease of H phases from 1:10 to 1:30 at 48 hours Rilpivirine (Fig?3.) The variations in cell cycle distribution between the A549 and CIK treated A549 cells Rilpivirine were statistically ...http://elevateblogconference.com/background-adenocarcinoma-the-most-common-form-of-lung-malignancy-is-1/