Organ-specific cell division abnormalities caused by mutation in a general cell cycle regulator in C. elegans | Development
Cdks can also be negatively regulated by cyclin-dependent kinase inhibitors (CKIs); small polypeptides that bind to and inhibit ... cell cycle arrest in G1 or G0 (Pardee, 1989; Sherr, 1994). Coordination of cell cycle progression and arrest may depend on the ... One important gene family involved in such control is the p21/p27/p57 class of negative cell cycle regulators. Loss of function ... Koff, A. and Polyak, K. (1995). p27KIP1, an inhibitor of cyclin-dependent kinases. Prog. Cell Cycle Res. 1, 141-147. ...http://dev.biologists.org/content/129/9/2155
KAISO, a critical regulator of p53-mediated transcription of CDKN1A and apoptotic genes | PNAS
... cyclin-dependent kinase inhibitor 1) and apoptosis genes, subsequently inducing cell cycle arrest and potent apoptosis. KAISO ... Analogously, in Kaiso KO mouse embryonic fibroblast cells, p53-to-promoter binding and up-regulation of p21 and apoptosis gene ... Cell-specific Kaiso (ZBTB33) Regulation of Cell Cycle through Cyclin D1 and Cyclin E1 ... Moreover, the p53 with this particular acetylation pattern shows increased DNA binding and potently induces cell cycle arrest ...http://www.pnas.org/content/111/42/15078
Ectopic Expression of Interferon Regulatory Factor-1 Promotes Human Breast Cancer Cell Death and Results in Reduced Expression...
The p21/WAF1/CP1 cyclin-dependent kinase inhibitor is essential for DNA damage-induced cell cycle arrest, and its transcription ... p21 was up-regulated in IRF-1-infected MDA-MB-468 and SK-BR-3 cells. Recently, direct binding of IRF-1 to the IRF-E binding ... P21 interacts with cyclin/cyclin-dependent kinase complexes and may inhibit their activity (37 , 38) , resulting in cell cycle ... Barr virus bZIP transcription factor Zta causes G0/G1 cell cycle arrest through induction of cyclin-dependent kinase inhibitors ...http://cancerres.aacrjournals.org/content/64/22/8381
Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo
... the cyclin-dependent kinases (Cdks), and the expression of their cyclin partners, whereas inhibitors of Cdks, including p21 and ... a cyclin-Cdk inhibitor, links transforming growth factor-β and contact inhibition to cell cycle arrest," Genes and Development ... Cells were harvested by trypsinization, washed twice with PBS, and resuspended in 500 μL of binding buffer. Cell suspensions ... by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in ...https://www.hindawi.com/journals/ecam/2012/818261/
p21 binding to PCNA causes G1 and G2 cell... & related info | Mendeley
... inhibitors is its ability to associate with the proliferating cell nuclear antigen (PCNA), an auxiliary fa... ... A unique feature of p21 that distinguishes it from the other cyclin-dependent kinase (CDK) ... Cell cycle. *Cyclin-dependent kinase. *Growth arrest. *PCNA. *p21(CIP1/WAF1). Get free article suggestions today. Mendeley ... p21 binding to PCNA causes G1 and G2 cell cycle arrest in p53-deficient cells. *Cayrol C ...https://www.mendeley.com/research-papers/p21-binding-pcna-causes-g1-g2-cell-cycle-arrest-p53deficient-cells/
Cell Cycle Regulation of Stem Cells by MicroRNAs | SpringerLink
Cyclin dependent kinase inhibitor (CDKI) proteins including p21/Cip1, p27/Kip1 and p57/Kip2, block the activity of cyclin E- ... Cyclin dependent kinase proteins (CDK) tightly regulate the progression of the cell cycle. A CDK binds to its regulatory cyclin ... In this regard, miR-16 has been shown to inhibit MSC proliferation and induce cell cycle arrest by targeting cyclin E . ... MiRNAs and Cell Cycle Regulation of Stem Cells. Embryonic Stem Cells (ESCs). The duration of the cell cycle is variable between ...https://link.springer.com/article/10.1007/s12015-018-9808-y
Circles reshaping the RNA world: from waste to treasure | Springer for Research & Development
... while p21 (cyclin-dependent kinase inhibitor 1) restrains these activities and arrest cell cycle progression . The ... Circ-Foxo3 was shown to bind with the cell cycle proteins CDK2 and p21 to form a ternary complex. Normally, CDK2 (cyclin- ... A novel cyclinE/cyclinA-CDK inhibitor targets p27(Kip1) degradation, cell cycle progression and cell survival: implications in ... dependent kinase 2) is a well-known cell cycle facilitator interacting with cyclinA and cyclinE , ...https://rd.springer.com/article/10.1186/s12943-017-0630-y
The Dual Role of TGF in Human Cancer: From Tumor Suppression to Cancer Metastasis
... and induces cell cycle arrest in the G1 phase by increasing the expression of the small cyclin-dependent kinase inhibitors p15 ... inhibits CDK4/6 and displaces p21 and p27 from their preexisting CDK4/6 complexes allowing them to bind and inhibit CDK2-cyclin ... Cell Cycle Inhibition. Cell cycle progression is controlled by intracellular protein kinases, called cyclin dependent kinases ( ... calcium-calmodulin-dependent protein kinase II , cyclin-dependent kinase CDK2/4 , casein kinase , protein kinase C ...https://www.hindawi.com/journals/isrn/2012/381428/
Other Scholarly Content | Pitx2 expression induces cell cycle exit and p21 expression in neural stem cells | ID: wh246x81h |...
... including cyclins, cyclin dependent kinases and their inhibitors. In this study, we exogenously expressed the homeodomain ... The G1 phase of the cell cycle is mostly associated with cell cycle arrest and cell differentiation. Cell cycle progression and ... increase in expression of the cell cycle inhibitor p21Cip. In addition, Pitx2 bound directly to the p21Cip promoter as assessed ... A search for potential cell cycle inhibitors responsible for such cell cycle exit of NSCs revealed that Pitx2 expression caused ...https://ir.library.oregonstate.edu/concern/defaults/wh246x81h
Transforming Growth Factor β Mimetics: Discovery of 7-[4-(4-Cyanophenyl) phenoxy]-Heptanohydroxamic Acid, a Biaryl Hydroxamate...
Because one of the mechanisms that leads to G1 arrest is the induction of the cyclin-dependent kinase inhibitors, e.g., p21cip1 ... Effect of A-161906 and TGF-β on Cell Cycle in Mv1Lu Cells.. The effects of A-161906 were evaluated on cell cycle progression in ... In this regard, studies with A-161906 in highly Smad-dependent processes, e.g., activation of the Smad binding element/CAGA-Lux ... Consistent with the G1-S arrest, A-161906 induced the expression of the cyclin-dependent kinase inhibitor p21waf1/cip1. A- ...http://mct.aacrjournals.org/content/1/10/759
CDKN1A / WAF1 / p21 Antibody (aa117-146, FITC) for IHC, WB/Western, ELISA LS-C208353
P21 antibody LS-C208353 is an FITC-conjugated rabbit polyclonal antibody to p21 (CDKN1A / WAF1) from human and mouse. Validated ... About CDKN1A / WAF1 / p21. CDKN1A / WAF1 / p21 is a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and ... through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. ... inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The ...https://www.lsbio.com/antibodies/cdkn1a-antibody-waf1-antibody-p21-antibody-aa117-146-fitc-elisa-ihc-wb-western-ls-c208353/217007
Cell Cycle Protein Expression in Vascular Smooth Muscle Cells In Vitro and In Vivo Is Regulated Through Phosphatidylinositol 3...
As previously shown by others,21 upregulation of the cyclin-dependent kinase inhibitor p21Cip1 was prevented as well, further ... RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs. Cell. 1994;78 ... and arrested the cells in vitro before S phase. In contrast to findings in other cell types, growth factor- or balloon injury- ... cyclin D1, cyclin E, cyclin-dependent kinase 1, cyclin-dependent kinase 2, proliferating cell nuclear antigen, and p21Cip1 in ...http://atvb.ahajournals.org/content/21/7/1152
Cytotoxic effect of 5-aminoimidazole-4-carboxamide-1-β-4-ribofuranoside (AICAR) on childhood acute lymphoblastic leukemia (ALL)...
We found that treatment with AICAR inhibited cell proliferation, induced cell cycle arrest in G1-phase, and apoptosis in CCRF- ... These effects were abolished by treatment with the adenosine kinase inhibitor 5'-iodotubericidin prior to addition of AICAR ... We evaluated the effects of the AMPK activator AICAR on cell growth, cell cycle regulators and apoptosis of various childhood ... of the PI3K/Akt pathway may represent a compensatory survival mechanism in response to apoptosis and/or cell cycle arrest. ...https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-6-46
One Function-Multiple Mechanisms: The Manifold Activities of p53 as a Transcriptional Repressor
... and in cell-cycle control like the cyclin dependent kinase (CDK) inhibitor p21CIP1  or the inhibitor of Cyclin B/Cdc2 ... A. Vigneron, J. Cherier, B. Barré, E. Gamelin, and O. Coqueret, "The cell cycle inhibitor p21 binds to the myc and cdc25A ... On the other hand, miRNA-mediated repression of cell-cycle genes may also contribute to cell-cycle arrest. miRNA-107 is ... "The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases," Cell, vol. 75, no. 4, pp. 805-816, ...https://www.hindawi.com/journals/bmri/2011/464916/
OriGene - CDKN1A (AY008263) shRNA
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin- ... through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. ... Transient overexpression lysate of cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 2 ... cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at ...http://www.origene.com/shRNA/TF305469.aspx
... the transcriptional activation of p21WAF1, an inhibitor of cyclin-dependent kinases (6) . Biochemical mechanisms underlying ... Wild-type p53 is a critical cellular gatekeeper for growth and division that mediates DNA damage-induced cell-cycle arrest and ... Smac promotes caspase activation by binding to inhibitors of apoptosis proteins (IAPs) and suppressing their antiapoptotic ... Whole-Cell Extract and Cytosolic and Mitochondrial Fraction Preparation.. Cells were lysed in 100 μl of cold CHAPS Cell Extract ...http://cancerres.aacrjournals.org/content/64/9/3022
Histone Deacetylase Inhibitor FK228 Activates Tumor Suppressor Prdx1 with Apoptosis Induction in Esophageal Cancer Cells |...
... in both T.Tn and TE2 cells. Cyclin-dependent kinase inhibitor p21WAF1 was clearly up-regulated and it has been reported to play ... both in G1 and G2-M cell cycle arrest (29). Recent studies also reported that HDACIs exert cell cycle arrest by inducing p21 ... is an Abl SH3-binding protein and a physiological inhibitor of c-Abl tyrosine kinase activity. Genes Dev 1997;11:2456-67. ... activate transcription of the cyclin-dependent kinase inhibitor p21WAF1 (15, 17, 18) and induction of p21WAF1 by HDACIs ...http://clincancerres.aacrjournals.org/content/11/21/7945
P21 - Wikipedia
"Survival and cell cycle control in early hematopoiesis: role of bcl-2, and the cyclin dependent kinase inhibitors P27 and P21 ... also found that γ-irradiation of fibroblasts induced a p53 and p21 dependent cell cycle arrest, here p21 was found bound to ... p21 is a potent cyclin-dependent kinase inhibitor (CKI). The p21 (CIP1/WAF1) protein binds to and inhibits the activity of ... also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a cyclin-dependent kinase inhibitor (CKI) ...https://en.wikipedia.org/wiki/P21
Phenotypic and Molecular Characterization of MCF10DCIS and SUM Breast Cancer Cell Lines
p21 is a potent cyclin-dependent kinase inhibitor and inhibits the activity of cyclin-CDK2 or -CDK1 complexes, and thus ... through which this protein mediates the p53-dependent cell cycle phase arrest in response to a variety of stress stimuli. p21 ... In cells entering the cell cycle, extracellular signals induce the expression of D-type cyclins, which bind to and activate ... and the kinase activity of CDK4 is suppressed by p16INK4a (p16). Cyclin-dependent kinase inhibitor 2A, (CDKN2A, ) also known as ...https://www.hindawi.com/journals/ijbc/2013/872743/
p21 Antibody (Monoclonal, HJ21)
p21 Monoclonal Antibody from Invitrogen for Western Blot, Immunofluorescence and Immunohistochemistry applications. This ... cyclin-dependent protein serine/threonine kinase inhibitor activity. protein binding. cyclin-dependent protein kinase ... signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Ras protein signal transduction ... Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, ...https://www.thermofisher.com/antibody/product/p21-Antibody-clone-HJ21-Monoclonal/AHZ0422
The Novel p21 Polymorphism p21G251A Is Associated with Locally Advanced Breast Cancer | Clinical Cancer Research
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 1993;75:805-16. ... Luo Y, Hurwitz J, Massague J. Cell-cycle inhibition by independent Cdk and Pcna binding domains in P21(Cip1). Nature 1995;375: ... The cyclin-dependent kinase inhibitor p21 (CIP1, WAF1, CDKN1A) is thought to be the main executor of p53-induced growth arrest ... P53-dependent inhibition of cyclin-dependent kinase-activities in human fibroblasts during radiation-induced G1 arrest. Cell ...http://clincancerres.aacrjournals.org/content/12/20/6000
Gene Ontology Classifications
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin- ... through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. ... cyclin-dependent kinase inhibitor 1A (P21). MGI:104556 Go Annotations as Summary Text (Tabular View) (GO Graph). Summary from ... cyclin-dependent kinase2 or cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at ...http://www.informatics.jax.org/go/marker/MGI:104556
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 1993;75:805-16. ... There were also other cell cycle arrests that occurred in human cells following ionizing radiation. Throughout S phase, ... Participation of ATM in insulin signalling through phosphorylation of eIF-4E binding protein 1 (4E-BP1). Nat Cell Biol 2000;2: ... perhaps the best known of which is the p21 cyclin-dependent kinase inhibitor discovered by the Vogelstein and Elledge ...http://mcr.aacrjournals.org/content/6/4/517
Brain Sciences | Free Full-Text | Ethanol Neurotoxicity in the Developing Cerebellum: Underlying Mechanisms and Implications |...
Ethanol's harmful effects include neuronal cell death, impaired differentiation, reduction of neuronal numbers, and weakening ... In this period neuronal maturation and differentiation begin and neuronal cells start migrating to their ultimate destinations ... Ethanol Alters Cell Cycle Progression. Cyclin-dependent kinases (CDKs) are required for neural development and differentiation ... and Guanine dissociation inhibitors (GDIs). Downstream, important effectors for active Rac1 and Cdc42 are the p21-activated ...http://mdpi.com/2076-3425/3/2/941/htm
Molecular Vision: Inhibition of proliferation of rat lens epithelial cell by overexpession of KLF6
KLF6 also directly interacts with cyclin D1 to suppress cyclin-dependent kinase 4 and causes cell cycle arrest . ... p21 WAF1/Cip1), an inhibitor of the cyclin-dependent kinases, in both cultured cells and a transgenic mouse model . ... a novel DNA-binding zinc finger protein that binds to the GC-rich binding sites of the HIV-1 promoter. J Biochem. 1998; 124:389 ... Conclusions: pKLF6 cDNA inhibited cell proliferation and decreased cell viability of LEC by unregulation of p21 and p27 kip1. ...http://www.molvis.org/molvis/v17/a122/