Mechanisms of targeting the MDM2-p53-FOXM1 axis in well-differentiated intestinal neuroendocrine tumors - Abstract -...
Results: The midgut wild type cell line KRJ-I responded to the treatment with cell cycle arrest and apoptosis. By gene ... Finally, we analyzed the anti-tumor effect of nutlin-3a in a xenograft mouse model in vivo. During the study, the tumor volume ... in p53 wild type and mutant GEP-NEN cell lines by proliferation assay, flow cytometry, immune fluorescence, western blot and ... KRJ-I-derived xenograft tumors showed a significantly decrease tumor growth upon treatment with nutlin-3a in vivo. Furthermore ...https://www.karger.com/Article/Abstract/481506
FHL2 | Cancer Genetics Web
We showed that suppression of FHL2 by rAAV-shRNA induced G0/G1 cell cycle arrest and inhibited cell growth. Apoptosis-related ... The use of isogenic cell lines and a xenograft model that mimics the transition from androgen-stimulated to castration- ... To examine the effects of Ets-1 upon cell migration, a wound assay was performed, and demonstrated that wounds were completely ... A strong anti-tumorigenic effect of rAAV-FHL2-shRNA was shown in nude mice and this antitumor effect was enhanced when combined ...http://www.cancerindex.org/geneweb/FHL2.htm
Modulation of Hsp90 Function by Ansamycins Sensitizes Breast Cancer Cells to Chemotherapy-induced Apoptosis in an RB- and...
Instead, these cells undergo cell cycle arrest in mitosis followed by apoptosis (10) . 17-AAG has antitumor activity in a ... 17-AAG has antitumor activity in cell culture and animal xenograft models and is currently in clinical trial. It causes an RB- ... 17-AAG caused sensitization of SKBr-3 cells to Taxol as demonstrated by several assays of apoptosis. SKBR-3 cells are RB- ... In cells with mutated RB, 17-AAG caused cell cycle arrest in mitosis, followed by apoptosis. In these cells, the 17-AAG-induced ...http://clincancerres.aacrjournals.org/content/7/8/2228?ijkey=2b161c0e04ddd997f2af2f4b9f43eef3bfd60322&keytype2=tf_ipsecsha
FW-04-806 inhibits proliferation and induces apoptosis in human breast cancer cells by binding to N-terminus of Hsp90 and...
Quantitative Real-time PCR and xenograft models on the research of FW-04-806 antitumor mechanism, through the HER2- ... In breast cancer cell lines, FW-04-806 inhibits cell proliferation, caused G2/M cell cycle arrest, induced apoptosis, and ... The result is consistent with cell proliferation assay and in vitro apoptosis assay applied for SKBR-3 and MCF-7. Furthermore, ... Importantly, FW-04-806 displays a better anti-tumor effect in HER2-overexpressed SKBR3 tumor xenograft model than in HER2- ...https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-150
Mechanism of PFK15-induced cell cycle arrest in G0/G1 p | Open-i
Mechanism of PFK15-induced cell cycle arrest in G0/G1 phase.Cells were treated with various concentration of PFK15 for 24 h. ... apoptosis and cell cycle progression in gastric cancer cells were evaluated by cytotoxicity and apoptosis assays, flow ... and a xenograft tumor model was used to verify the therapeutic effect of PFK15 in vivo. Results showed that PFK15 inhibited the ... The objective of this study was to investigate the antitumor activity of PFK15 (1-(4-pyridinyl)-3-(2-quinolinyl)-2-propen-1-one ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC5036843_pone.0163768.g003&req=4
ROS generation mediates PA-activated apoptotic pathways | Open-i
a NCI-H23 and NCI-H460 cells were treated with PA (20, 40 and 80 µM) for 24 h. The protein levels of ... colony formation and EdU proliferation assays. Flow cytometric analysis was used to detect cell cycle changes. Apoptosis was ... Results: PA exhibited anti-tumor effects in vitro accompanied by induction of G2/M phase arrest and apoptosis in NCI-H23 and ... Bottom Line: The in vivo efficacy of PA was measured using a NCI-H23 xenograft model in nude mice.Moreover, blockage of ROS ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4524283_12935_2015_230_Fig3_HTML&req=4
Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo
"Perillyl alcohol and perillic acid induced cell cycle arrest and apoptosis in non small cell lung cancer cells," Cancer Letters ... A549 xenografts by inducing apoptosis, as confirmed by TUNEL assay. Collectively, these data provide insight into the molecular ... antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell ... Terpinen-4-ol Inhibits A549 Tumor Growth in a S.C. Tumor Model. The prominent inhibitory effect of terpinen-4-ol on NSCLC cell ...https://www.hindawi.com/journals/ecam/2012/818261/
SEM image and in vitro release profile of 9NC loaded li | Open-i
Xenograft Model Antitumor Assays*. Minor. *Animals. *Apoptosis/drug effects. *Cell Cycle/drug effects ... 9NC and 9NC-LP can inhibit HCC growth via cell cycle arrest and induction of apoptosis. 9NC-LP has a more potent anti-tumor ... 9NC and 9NC-LP can inhibit HCC growth via cell cycle arrest and induction of apoptosis. 9NC-LP has a more potent anti-tumor ... 9NC and 9NC-LP can inhibit HCC growth via cell cycle arrest and induction of apoptosis. 9NC-LP has a more potent anti-tumor ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3111480_pone.0021064.g001&req=4
Synergistic Activity of Sorafenib and Sulforaphane Abolishes Pancreatic Cancer Stem Cell Characteristics | Cancer Research
... and acts as an antioxidant by increasing reduced glutathione levels as well as inducing cell cycle arrest and apoptosis. In ... In the present study, we used in vitro assays and in vivo xenograft models to examine the effects of SO on the pancreatic CSC ... Sorafenib (SO), a novel multikinase inhibitor, also known as BAY 43-9006 or Nexavar, has shown promising antitumor activity in ... Xenograft tumor models. MIA-PaCa2 tumor cells (2 × 107 in 200 μL of PBS) were injected s.c. into the left and right flanks of 4 ...http://cancerres.aacrjournals.org/content/70/12/5004
Simvastatin-induced cell cycle arrest through inhibition of STAT3/SKP2 axis and activation of AMPK to promote p27 and p21...
Moreover, we established a HepG2 tumor-bearing xenograft animal model to examine the antitumor effects of simvastatin in vivo. ... Simvastatin inhibits cell growth and induces apoptosis and G0/G1 cell cycle arrest in hepatic cancer cells. Int J Mol Med 2010 ... DNA content assay. Cell cycle distributions were determined by DNA content assay. HepG2 and Hep3B cells were seeded into six- ... Cell cycle arrest and apoptosis induction in hepatocellular carcinoma cells by HMG-CoA reductase inhibitors. Synergistic ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5386458/
Pachymic acid induces apoptosis via activating ROS-dependent JNK and ER stress pathways in lung cancer cells | Cancer Cell...
... colony formation and EdU proliferation assays. Flow cytometric analysis was used to detect cell cycle changes. Apoptosis was ... The in vivo efficacy of PA was measured using a NCI-H23 xenograft model in nude mice. PA exhibited anti-tumor effects in vitro ... accompanied by induction of G2/M phase arrest and apoptosis in NCI-H23 and NCI-H460 lung cancer cells. Mechanistically, our ... and inhibited cell proliferation and induction of apoptosis of tumor cells in tumor xenograft tissues. In summary, our study ...https://cancerci.biomedcentral.com/articles/10.1186/s12935-015-0230-0
Evaluation of Selective γ-Secretase Inhibitor PF-03084014 for Its Antitumor Efficacy and Gastrointestinal Safety to Guide...
Induction of cell cycle arrest and apoptosis by PF-03084014 in T-ALL cells. A, HPB-ALL cells were treated with indicated ... Subcutaneous xenograft models in athymic mice. Cells for implantation into athymic mice were harvested and pelleted by ... Cell-based proliferation assay. Cells were seeded in 96-well plates at 2,000 (Sup-T1, Jurkat, and DND-41) or 10,000 (HPB-ALL or ... PF-03084014 caused cell growth inhibition of several T-ALL cell lines via cell cycle arrest and induction of apoptosis. PF- ...http://mct.aacrjournals.org/content/9/6/1618
Enhancement of pomalidomide anti-tumor response with ACY-241, a selective HDAC6 inhibitor - pdf descargar
... is shown to synergize with pomalidomide in in vitro assays through promoting greater apoptosis and cell cycle arrest. ... Furthermore, utilizing a multiple myeloma in vivo murine xenograft model, combination treatment with pomalidomide and ACY-241 ... Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer - Shu Ruan, Feng Jia, and Jianbo Li ... Fracture Mechanics Models for Brittle Failure of Bottom Rails due to Uplift in Timber Frame Shear Walls - Joergen L. Jensen, ...http://libros.duhnnae.com/2017/jun9/149863370214-Enhancement-of-pomalidomide-anti-tumor-response-with-ACY-241-a-selective-HDAC6-inhibitor.php
Crocin Exhibits Antitumor Effects on Human Leukemia HL-60 Cells In Vitro and In Vivo
HL-60 cells were treated by crocin, and cell proliferation, apoptosis, and cell cycle profiles were examined by MTT assay, AO/ ... Nude mice xenograft models were established by injecting HL-60 cells (. /0.2 mL) subcutaneously on the back of the right ... Figure 2: Crocin induces apoptosis and cell cycle arrest of HL-60 cells. (a) HL-60 cells were treated by crocin at the ... Crocin Induces Apoptosis and Cell Cycle Arrest of HL-60 Cells. To determine whether crocin inhibits the proliferation of HL-60 ...https://www.hindawi.com/journals/ecam/2013/690164/
Frontiers | Para-Toluenesulfonamide Induces Anti-tumor Activity Through Akt-Dependent and -Independent mTOR/p70S6K Pathway:...
... two CRPC cell lines, through p21- and p27-independent G1 arrest of cell cycle in which cyclin D1 was down-regulated and Rb ... Moreover, the tumor xenograft model showed that PTS inhibited tumor growth with a T/C (treatment/control) of 0.44 and a 56% ... leading to apoptosis. PTS inhibited the phosphorylation of m-TOR, 4E-BP1 and p70S6K in both cell lines. Overexpression of ... two CRPC cell lines, through p21- and p27-independent G1 arrest of cell cycle in which cyclin D1 was down-regulated and Rb ...https://www.frontiersin.org/articles/10.3389/fphar.2018.01223/full
POLD1 | Cancer Genetics Web
These findings indicate that Dp inhibits cell proliferation, induces cell cycle arrest and apoptosis in glioma cells, and is a ... tumorigenicity assay and a mouse model. Cell cycle distribution was examined with flow cytometric analysis, and cell cycle- ... The antitumor efficacy of NHPI in vivo was tested in BT-20 xenografts. The underlying antitumor mechanisms of NHPI in vitro and ... Silencing Hec1 markedly suppressed proliferation, promoted apoptosis and induced cell-cycle arrest in G2/M-phase in PCa cells. ...http://www.cancerindex.org/geneweb/POLD1.htm
CPT exhibits highly S phase-specific cytotoxicity and induces G2-M cell cycle arrest (11) . A replication fork collision model ... This conclusion is also supported by results from our studies using the apoptosis assay, because P388/CPT45 cells were more ... Antitumor Activity against scid Mice Carrying Human Tumor Xenografts.. CB17/Icr female scid mice were implanted with a single ... Assay for TOP1-SUMO Conjugates.. RPMI-8402 cells (106/sample) were treated with drug for 15 min at 37°C. Cells were pelleted ...http://cancerres.aacrjournals.org/content/63/23/8400
... which inhibits the proteasome of glioma cells with the subsequent induction of cell cycle arrest and apoptosis. Of note, short- ... delays the growth of malignant glioma cells was subsequently assessed in vivo in an intracerebral glioma xenograft mouse model ... Cell viability was assessed by the MTT assay for K-562 cells or cell density by crystal violet staining for all other cell ... 5B). Because local application of antitumor compounds is also a feasible delivery method, we additionally checked whether an ...http://clincancerres.aacrjournals.org/content/15/21/6609
Protocols and Video Articles Authored by Xiao-Guang Chen
Antitumor activity was observed by Renca xenograft model in vivo. The effect of T03 on cell cycle and apoptosis were measured ... T03 induced cell apoptosis and cell cycle arrest in HCC. Moreover, it proved that T03 reduced the tumor weight with the rate of ... The abilities of cell motility and adhesion in BxPC3 cells were detected by MTT assay and wound healing assay, respectively. ... Induction of Cell Cycle Arrest by GL331 Via Triggering an ATM-dependent DNA Damage Response in HepG2 Cells Journal of Asian ...https://www.jove.com/author/Xiao-Guang_Chen
Histone Deacetylase Inhibitor FK228 Activates Tumor Suppressor Prdx1 with Apoptosis Induction in Esophageal Cancer Cells |...
Cell cycle analysis and terminal deoxynucleotidyl transferase-mediated nick-end labeling assay. For cell cycle analysis, cells ... Xenograft model of real-time quantitative PCR for Prdx1 gene. T.Tn (2.5 × 106 cells) and TE2 (2.5 × 106 cells) were injected ... Taken together, we propose a novel antitumor mechanism of FK228 that induces cell growth arrest and/or apoptosis induction ... both in G1 and G2-M cell cycle arrest (29). Recent studies also reported that HDACIs exert cell cycle arrest by inducing p21 ...http://clincancerres.aacrjournals.org/content/11/21/7945
MGCD0103, a novel isotype-selective histone deacetylase inhibitor, has broad spectrum antitumor activity in vitro and in vivo |...
Induction of Cell Cycle Arrest and p21 Expression in Human Cancer Cell Lines In vitro. Cell cycle profiles of human cancer cell ... Broad Spectrum Antitumor Activity in Human Tumor Xenograft Models and Induction of Histone Acetylation in Tumors In vivo. To ... associated with growth arrest and apoptosis of human cancer cell lines. These effects were selective to cancer cells as they ... Cell Viability Assay. Cells in 96-well plates were incubated with compounds at various concentrations for 72 h at 37°C in 5% CO ...http://mct.aacrjournals.org/content/7/4/759
... resulting in decreased proliferation and increased apoptosis (22). This was also shown in an in vivo xenograft model of canine ... and have shown efficacy in hematologic and solid tumors through inhibition of proliferation by cell cycle arrest, effects on ... Traditional preclinical murine models often fail to accurately predict the antitumor activity or toxicity observed in human ... Controls for the valproic acid assay were purchased from Cliniqa. Anti-acetyl-histone H3 and H4 and total histone H3 and H4 ...http://clincancerres.aacrjournals.org/content/16/19/4832
Foretinib (GSK1363089), an Orally Available Multikinase Inhibitor of c-Met and VEGFR-2, Blocks Proliferation, Induces Anoikis,...
... reduced proliferation mediated by a G2-M cell-cycle arrest; (iii) induced cell death through a 2-step mechanism in which cells ... and in 2 xenograft mouse models, it reduced tumor burden through inhibition of angiogenesis and induction of apoptosis. ... Primary cells, 3D model, adhesion, migration, and invasion assay. Primary human peritoneal mesothelial cells and the 3D omental ... Our data suggest that anoikis induction contributes to the potent antitumor effects of the inhibitor. Characterization of the ...http://clincancerres.aacrjournals.org/content/17/12/4042.long
Valproic Acid Induces Notch1 Signaling in Small Cell Lung Cancer Cells
Valproic acid induces apoptosis and cell cycle arrest in poorly differentiated thyroid cancer cells. J Clin Endocrinol Metab. ... Furthermore, we have previously demonstrated in a mouse xenograft model of NET disease that non-toxic concentrations of VPA can ... In order to assess the impact of VPA on SCLC cells, we utilized the MTT cell proliferation assay, a measure cell mitochondrial ... that VPA may also activate Notch1 signaling in SCLC cells with subsequent anti-tumor effects. To test this hypothesis we ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2900385/
Synergistic killing of lung cancer cells by cisplatin and radiation via autophagy and apoptosis
Computerized video time lapse study of cell cycle delay and arrest, mitotic catastrophe, apoptosis and clonogenic survival in ... Lewis cells were injected into mice to establish the xenograft model. When the xenografts had grown to the same size, the mice ... A) Cell viability was detected by MTT assay following treatment of the A549 cells with various concentrations of cisplatin. (B ... On one hand, autophagy exhibits an antitumor role (9); while on the other hand, autophagy, an adaptive response, protects tumor ...https://www.spandidos-publications.com/ol/7/6/1903