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*  US5945337A - Method for culturing CD34+ cells in a serum-free medium - Google Patents
The recipe for the formulation is given, which provides a medium suitable for the proliferation and differentiation of CD34 + ... cells purified from normal bone marrow, peripheral blood of patients treated with cytokines, and umbilical cord blood is ... A serum-free medium which supports the proliferation and differentiation of CD34 + ... Animal cell culture medium containing a plant-derived nutrients WO1998016629A1 (en) 1996-10-11. 1998-04-23. Life Technologies, ...
  https://patents.google.com/patent/US5945337
*  Cell Culture Media
SFM4Transfx-293 Media, a serum-free, animal-derived component free media. ... HyClone™ SFM4Transfx-293 Media. Promote transfection using lipofection and support growth of HEK 293 cultures with HyClone™ ... Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin-free medium specifically designed for high- ...
  https://www.fishersci.com/us/en/products/I9C8K0EE/cell-culture-media.html
*  Rogers, CA - Patent applications
The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or ... culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and ... The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells ( ... Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the ...
  http://www.patentsencyclopedia.com/inventor/rogers-ca-3/
*  US20080145893A1 - Method for producing a recombinant protein at high specific productivity, high batch yield and high...
... batch yield and volumetric yield to be an economic alternative to stable cell lines. The inventors improved on the methodology ... and labor-consuming step of creating stable cell lines. Production methods based on transient gene expression are advantageous ... exceeding 35 pg per cell per day), batch yield (exceeding 700 mg/l) and volumetric yield. ... Yet, most current production methods in mammalian cells involve the time- ...
  https://patents.google.com/patent/US20080145893A1/en
*  IJMS | Free Full-Text | Enhanced Cell Growth of Adipocyte-Derived Mesenchymal Stem Cells Using Chemically-Defined Serum-Free...
The animal factor included in culture media also limits clinical application. In this study, adipose-derived MSCs showed a ... In addition, MSCs cultured in STK2 showed a reduced senescence rate, small and homogenous cell size, and were more genetically ... Taken together, these results suggest that culture using STK2 medium offers many advantages through which it is possible to ... Tri-lineage differentiation analyses showed that MSCs cultured in STK2 were superior to those cultured in DMEM/FBS. ...
  http://www.mdpi.com/1422-0067/18/8/1779
*  General T cells - T cells - Applications - Miltenyi Biotec
T cell maintenance. TexMACS Medium is an optimized, serum-free cell culture medium developed for reliable T cell and Treg cell ... Chemically-defined and without animal-derived components, it allows for the highest reproducibility in human and mouse cell ... Cells were cultured either in TexMACS Medium (+ 10% serum) or RPMI (+ 10% serum) for 5 days . Analysis of lineage-specific ... Comparison of expansion rates of human CD4+ and CD8+ T cells in TexMACS Medium or competitor's T cell medium using the T Cell ...
  https://www.miltenyibiotec.com/UN-en/applications/t-cells/general-t-cells.html
*  Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research | Sigma-Aldrich
Addition of buffered salt solutions or serum-free culture medium stops labeling by causing rapid dye aggregation. However, ... In systems where animal-derived proteins must be avoided, recombinant albumin may be an acceptable substitute. ... Right: Suspension cultures of sorted PKH26pos or PKH26neg cells (~300 cells/secondary mammosphere).. B. Consistent with other ... transfected immune cells22,23, antigen presenting cells24, regulatory T cells23, and more. The ability to label almost any cell ...
  https://www.sigmaaldrich.com/technical-documents/articles/biowire/cell-tracking-lipophilic-membrane-dyes.html
*  Scientific Program | Cell Therapy 2016 | San Antonio| USA | OMICS International Conferences
Here, we evaluated growth of bone marrow derived hMSCs with a variety of cell culture media formulations and serum-free ... Cell culture medium and supplements are critical factors of the scale up process, yet many processes currently contain animal- ... These cells have now been harvested, by surgical excision of a stimulated nerveand cultured in appropriate restrictive media. ... In general, embryo derived stem cells require special culture techniques, are not motile, and in culture form colonies. While ...
  https://cellgenetherapy.conferenceseries.com/2016/scientific-program.php?day=1&sid=2343&date=2016-05-19
*  Mesenchymal Stem Cell Culture | Sigma-Aldrich
... are adult stem cells, which have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal ... Low FBS (2%) cell culture media for human mesenchymal stem cells.. Complete Xeno-free medium that is free of animal-derived ... serum-free, and cost effective for human mesenchymal stem cell cultures.. Application Note: PLTMax® Human Platelet Lysate (hPL ... Low Serum. Xeno-Free. GMP Grade. Human Mesenchymal-LS Expansion Medium. Human Mesenchymal-XF Expansion Medium. Stemline® ...
  https://www.sigmaaldrich.com/life-science/stem-cell-biology/mesenchymal.html
*  CryoStem™ Freezing Media - Stem Cell Freezing Medium
CryoStem Freezing Medium has been tested on hES cells and MSCs resulting in higher rates of cell adherence and pluripotent ... The culture of cells in animal component-free medium eliminates the risks associated with animal sera and serum-derived ... CryoStem Freezing Medium maintains xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment ... CryoStem Freezing Medium is a ready-to-use solution for the serum-free, animal component-free cryopreservation of human ...
  http://www.bioind.com/worldwide/cryostem-freezing-medium/
*  Patent US20030190748 - Serum free cultivation of primate embryonic stem cells - Google Patents
Also disclosed is a culture media containing fibroblast feeder layer and the fibroblast growth factor. ... These cells are cultured on a prolonged and stable basis in the presence of exogenously supplied fibroblast growth factor and ... in the absence of animal serum. Preferably there is also a fibroblast feeder layer. ... Disclosed herein are methods for culturing primate embryonic stem cells. ...
  http://www.google.com/patents/US20030190748?dq=5,870,513
*  Patent US7429567 - Sustained delivery of PDGF using self-assembling peptide nanofibers - Google Patents
Cardiomyocytes were plated at a density of 1.4 105 cells/cm2 overnight, cultured in serum-free DMEM for 24 hours, and then ... Once formed, membranes are stable in serum, aqueous solutions and cell culture medium. They can be made under sterile ... Smits, et al., "Neurotropic Activity of Platelet-Derived Growth Factor (PDGF): Rat Neuronal Cells Possess Functional PDGF beta- ... All animal protocols were approved by the Harvard Medical School Standing Committee on Animals. MI was produced in ...
  http://www.google.com/patents/US7429567?dq=7,117,286
*  Products | TNCbio
... serum free CHO culture. Chemically defined medium and CHO cells from TNCbio. CHOiX medium is free from animal and human derived ... replaces fetal bovine serum and other sera in cell cultures.. XerumFree™ is the best solution for achieving defined and ... Cell growth kits for defined and animal component free cell culture. XF Kit for endothelial cells. Complete kit for the culture ... TNCbio offers CHOiX medium for defined and consistent culture and expression of CHO cells. ...
  http://www.tncbio.com/products
*  DiVA - Søkeresultat
... cells based on Biovitrum's proprietary low protein serum-free medium without animal derived components (BVT4). The cells were ... Moreover, these cationic nanoparticles maintain their size in serum containing cell culture media, but not in serum free DMEM. ... MLN cells cultured ex vivo and treated with PalC had reduced CD4(+) CD25(+) T cells and an increased number of B220(+) CD19(+) ... glutamine and Biovitrum's proprietary low protein serum-free feed medium without animal derived components enriched with amino ...
  http://www.diva-portal.org/smash/resultList.jsf?dswid=-6003&searchType=SUBJECT&language=no&onlyFullText=false&af=%5B%5D&aq=%5B%5B%7B%22categoryId%22%3A%2211638%22%7D%5D%5D
*  Beneficial effects of astragaloside IV against angiotensin II-induced mitochondrial dysfunction in rat vascular smooth muscle...
The media were minced and plated into a cell culture bottle. The tissue was cultured in Dulbecco's modified Eagle's medium ( ... When the cells reached 70-80% confluence, and they were subjected to 24 h of starvation in serum-free DMEM. The cells were then ... Cell culture and treatments. The VSMCs derived from the thoracic aorta were obtained from male Sprague-Dawley (SD) rats ( ... The animal experimental procedures were carried out in accordance with the Experimental Animal Ethics rules and regulations of ...
  https://www.spandidos-publications.com/ijmm/36/5/1223?text=fulltext
*  Patent US6793677 - Method of providing cells and other biologic materials for transplantation - Google Patents
... the invention may be used as a way to provide disease-free disc tissue derived from animal sources, and is applicable as well ... cartilage cells, and so forth. Additional therapeutic substances like culture medium, growth factors, differentiation factors, ... In one disclosed example, living nucleus pulposis cells obtained from recently deceased human or animal donors are used to ... Although the description makes specific reference to human disc cells, ...
  http://www.google.com.au/patents/US6793677
*  Gs-Coupled Adenosine Receptors Differentially Limit Antigen-Induced Mast Cell Activation | Journal of Pharmacology and...
Bone Marrow-Derived Mast Cell Culture.. Bone marrow-derived mast cells (BMMCs) were isolated from the femurs of 8- to 12-week- ... BMMCs from A3−/− mice were cultured in vitro for 5 weeks in media containing murine IL-3 and stem cell factor. The expression ... dinitrophenyl-human serum albumin. HUCBMC. human umbilical cord blood-derived mast cell. IL. interleukin. LTB4. leukotriene B4 ... Here, using a panel of mice lacking various combinations of adenosine receptors, and mast cells derived from these animals, we ...
  http://jpet.aspetjournals.org/content/344/2/426.full
*  July 2010 Archives - BioProcess InternationalBioProcess International
... the biopharmaceutical industry has largely shifted to serum-free and/or chemically defined media in cell culture production ... Whether you are culturing animal cells in an R&D laboratory or in a pilot production facility, your ability to precisely ... Successful serum-free media formulations have been developed using plant-derived protein hydrolysates in combination with ... Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags ...
  http://www.bioprocessintl.com/category/2010/july-2010/
*  Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced...
The IPCs seeded on 24-well culture plates (Corning Costar) were washed with serum-free DMEM/F12, and exposed to culture media ... The BBB models were maintained in an EC medium [DMEM/F12 supplemented with 10% plasma derived serum (Animal Technologies Inc., ... The cells cultured in l-glucose (osmolarity control d-glucose 5.7 mM + l-glucose 35mM) for 5 days did not show OxSt or ... endothelial cell. ETC. electron transport chain. FACS. fluorescence-activated cell sorter. FBS. fetal bovine serum. GPI. ...
  http://jpet.aspetjournals.org/content/344/3/637.full
*  Enabling Technologies - BioProcess InternationalBioProcess International
... it maintains genetic stability in serum-free media while supporting virus production in suspension culture." Serum-free, animal ... "From the producer cell-line perspective, we see entirely new cell lines, such as EB66 cell line from Vivalis and SAFC. Derived ... Serum-free media and chemically defined supplements became the norm for cultures producing human therapeutics. Special ... with about a fifth choosing new culture media and another fifth divided between cell-line engineering and new cell lines. Of ...
  http://www.bioprocessintl.com/upstream-processing/cell-culture-media/enabling-technologies-348566/
*  Transcript</b>
... not from a live animal. To win, the lab-grown meat must have a taste and texture indistinguishable from real chicken flesh. ... Animal rights group PETA is offering a $1 million prize for the development of commercially-viable 'test-tube meat' - real meat ... and the main component of cell culture media is serum which has animal origin. So there's not any point to create animal-free ... tissue-engineered meat if you still use animal-derived serum. So the challenge is to design so-called defined cell culture ...
  https://www.npr.org/templates/transcript/transcript.php?storyId=89942776
*  Chemically defined medium - Wikipedia
Standard cell culture media are commonly supplemented with animal serum (such as fetal bovine serum, FBS) as a source of ... Serum-free media may contain undefined animal-derived products such as serum albumin (purified from blood), hydrolysates, ... of the over-all cost of the medium when calculated for large-scale cultures. There are increasing concerns about animal ... Reduced-serum media (commonly 1-5% FBS) Serum-free media (synonymous with Defined media) Protein-free media (no protein but ...
  https://en.wikipedia.org/wiki/Chemically_defined_medium
*  Organ culture - Wikipedia
Towell's II culture chamber). Since serum was found to be toxic, serum-free media were used, and the special apparatus ... Embryonic organ culture is an easier alternative to normal organ culture derived from adult animals. The following are four ... Another advantage is that cultured organs, created using the patients own stem cell allows for organ transplants would allow ... but adult organ culture will not survive on this medium. The culture of adult organs or parts from adult animals is more ...
  https://en.wikipedia.org/wiki/Organ_culture
*  Embryonic stem cell - Wikipedia
2005 detailed information about a new stem cell line that was derived from human embryos under completely cell- and serum-free ... The extracted inner cell mass was cultured on fibroblasts treated with mitomycin-c in a medium containing serum and conditioned ... Embryonic stem cells are derived from the inner cell mass of the early embryo, which are harvested from the donor mother animal ... These cells isolated from the teratocarcinoma replicated and grew in cell culture as a stem cell and are now known as embryonal ...
  https://en.wikipedia.org/wiki/Embryonic_stem_cell
*  Neural stem cell - Wikipedia
... been used to isolate and expand CNS stem cells by its ability to aggregate and proliferate hmNPCs under serum-free media ... "Generation and genetic modification of 3D cultures of human dopaminergic neurons derived from neural progenitor cells". Methods ... There are two ways to culture the hmNPCs, the adherent monolayer and the neurosphere culture systems. The neurosphere culture ... multipotent cells that generate the neurons and glia of the nervous system of all animals during embryonic development. Some ...
  https://en.wikipedia.org/wiki/Neural_stem_cell