*  FIC, flu article
Efficacy, safety, and immunogenicity of a Vero-cell-culture-derived trivalent influenza vaccine: a multicentre, double-blind, ... is a trivalent vaccine containing cold-adapted influenza vaccine viruses that infect and replicate in cells lining the ... Vero/CHOK1, a novel mixture of cell lines that is optimal for the rescue of influenza A vaccine seeds. J Virol Methods. 2013 ... BACKGROUND: The use of cell-culture technologies for the manufacture of influenza vaccines might contribute to improved strain ...
  http://flu.org.cn/en/article?action=search&category=INDUSTRY
*  Protocols and Video Articles Authored by Eva Wagner
Safety and Immunogenicity of a Vero Cell Culture-derived Whole-virus H5N1 Influenza Vaccine in Chronically Ill and ... Superior in Vitro Stimulation of Human CD8+ T-cells by Whole Virus Versus Split Virus Influenza Vaccines PloS One. 2014 , ... These data indicate that nonadjuvanted whole-virus cell culture-derived H5N1 influenza vaccines are suitable for immunizing ... In this study, the immunogenicity and safety of a nonadjuvanted cell culture-derived whole-virus H5N1 vaccine were assessed in ...
  https://www.jove.com/author/Eva_Wagner
*  U.S. National Institutes of Health | Netdata
Immunogenicity Study of an Inactivated Influenza Vaccine (Split Virus, Vero Cell Derived) to Prevent Culture Confirmed ... Stem Cells and Resynchronization Cardiac. Recruiting. No Results Available. Heart Failure. Procedure: Pacing and Stem Cells ... Influenza. Biological: Vero cell-derived, trivalent, seasonal influenza vaccine,Biological: Placebo: Phosphate-buffered saline ... Study of the Safety and Immunogenicity of an Adenovirus-based Tuberculosis Vaccine. Terminated. No Results Available. ...
  http://www.netdata.com/netsite/08750f34/us-national-institutes-of-health?p=4489
*  Interesting Late Stage Viral Vaccine Candidates
I decided to explore a handful of interesting viral vaccines that are in late stage development. ... After reviewing the list of vaccines in development, ... Baxter's vero cell culture derived Preflucel was approved in ... This approval was the first for a cell culture-based seasonal influenza vaccine in the United States. Instead of using the ... produced in Vero cells by a process that does not require supplementation with human or animal derived components in production ...
  http://cellculturedish.com/2013/05/interesting-late-stage-viral-vaccine-candidates/
*  Dr Ilya Sandra Perlingieri warns on the dangerous of the H1N1 bioweapon vaccine | Eugenics and pandemics
Dangers In The Shots - Components Of H1N1 Vaccines They are considered a biodefense agents. By Dr. Ilya Sandra Perlingieri © Dr ... Jeff Rense has posted up this report from Dr Ilya Sandra Perlingieri on the dangers of the H1N1 vaccines classified as ... Cultured cells are taken from this species of monkey through a process called "vero cell technology." This species of monkey ( ... Whole virus (H5N1) influenza vaccine, vero-celled derived. (See above.) Trometamol: Also known as Tris (or Tris buffer) or THAM ...
  https://birdflu666.wordpress.com/2009/08/04/dr-ilya-sandra-perlingieri-warns-on-the-dangerous-of-the-h1n1-bioweapon-vaccine/
*  Frontiers | Allopurinol reduces antigen-specific and polyclonal activation of human T cells | Immunology
Influenza virus, tetanus toxoid and Trypanosoma cruzi-derived antigens. Allopurinol attenuated CD69 upregulation after CD3 and ... The diminished T cell activation and cytokine production in the presence of allopurinol support a direct action of allopurinol ... Influenza virus, tetanus toxoid and Trypanosoma cruzi-derived antigens. Allopurinol attenuated CD69 upregulation after CD3 and ... The diminished T cell activation and cytokine production in the presence of allopurinol support a direct action of allopurinol ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2012.00295/full
*  Cell-based vaccine - Wikipedia
Doroshenko A, Halperin S. Trivalent MDCK cell culture-derived influenza vaccine Optaflu (Novartis Vaccines). Expert Reviews. ... The FDA has approved two mammalian vero cell based vaccines for rotavirus, Rotarix by GlaxoSmithKline and RotaTeq by Merck. ... Optaflu is nearly identical to Flucelvax; it is also produced in MDCK cells and targets the same Influenza subtypes. The main ... The potential use of cell culture techniques in developing viral vaccines, especially for the Influenza virus, has been widely ...
  https://en.wikipedia.org/wiki/Cell-based_vaccine
*  Binary options with metatrader
Development of gamma binary call option mammalian cell (Vero) derived candi- date influenza virus vaccine. ... Journal of Clinical Child Psychology, and E. 251 for A2780 cells and 34481 for OAW42 cells.Proc. Phys. The secretion of ... may be particularly suitable to the design and evaluation of culture-specific programs. Clinical Pediatrics, 15, 510515. ... 307,308 Comparison clinical studies of ts-1A2 vaccine and the cold- adapted Ann Arbor vaccines favored further development of ...
  http://imbc-med.ru/binary-options-with-metatrader-2.html
*  Cell culture - Wikipedia
... "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast ... Due to the H5N1 pandemic threat, research into using cell culture for influenza vaccines is being funded by the United States ... Primate cell lines Vero (African green monkey Chlorocebus kidney epithelial cell line) Mouse cell lines MC3T3 (embryonic ... Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Microcarrier Cell ...
  https://en.wikipedia.org/wiki/Cell_culture
*  Method for producing viruses and vaccines in serum-free culture - Patent # 5753489 - PatentGenius
... therefrom utilizes serum-free cultured vertebrate cells or vertebrate biomass aggregates to both eliminate the necessity to use ... A method for producing Influenza and other viruses and vaccines derived ... Continuous cell lines of serum-free VERO cells were propagated as monolayers and infected with Influenza virus containing ... A method for producing Influenza and other viruses and vaccines derived therefrom utilizes serum-free cultured vertebrate cells ...
  http://www.patentgenius.com/patent/5753489.html
*  Japanese Encephalitis Vaccines
... derived vaccine (JE-VAX [JE-MB]) and an inactivated Vero cell culture-derived vaccine (IXIARO [JE-VC]) (Table 1). JE-MB has ... JE-VC is an inactivated vaccine derived from the attenuated SA14-14-2 JEV strain propagated in Vero cells (Table 1) (14,159,160 ... influenza-like illness (13%), and fatigue (13%). One serious adverse event was reported in the JE-VC group; a male aged 50 ... TABLE 4. Immunogenicity of inactivated Vero cell culture-derived Japanese encephalitis vaccine (JE-VC) and hepatitis A vaccine ...
  https://www.cdc.gov/mmwr/preview/mmwrhtml/rr5901a1.htm
*  Novartis Is Celebrating. Should We? | HuffPost
... large-scale vaccine manufacturing facility in the U.S. Located in Holly Springs, North Carolina. ... "Novartis inaugurates large-scale US based cell-culture influenza vaccine manufacturing facility." November 24, 2009. http://www ... Examples include calf lymph for smallpox vaccines, African green monkey cells (AGMK cells and VERO cells) for polio vaccines, ... and shingles vaccines. Since 2000, new cells under investigation for making flu shots include cells derived from retinas of ...
  https://www.huffingtonpost.com/dr-sherri-tenpenny/novartis-is-celebrating-s_b_372551.html
*  hemagglutination inhibition tests
... and protective efficacy of a Vero-cell-culture-derived influenza vaccine, and assessed the correlation between vaccine efficacy ... West Nile Virus Life Cycle in Flavivirus-Resistant Cells. Pei Yong Shi; Fiscal Year: 2006 ... Safety and immunogenicity of inactivated, Vero cell culture-derived whole virus influenza A/H5N1 vaccine given alone or with ... Efficacy, safety, and immunogenicity of a Vero-cell-culture-derived trivalent influenza vaccine: a multicentre, double-blind, ...
  http://labome.ru/topics/diagnostic/diagnosis/laboratory/immunologic/serologic/hemagglutination-inhibition-tests-16562.html
*  'encephalitis virus western equine' Protocols and Video...
Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons', 'A Guide to Modern Quantitative ... Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells', 'Measuring ... Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells ... Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches ...
  https://www.jove.com/keyword/encephalitis+virus+western+equine
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Influenza, Human, Influenza in Birds, Influenza Vaccines, hemagglutinin, neuraminidase, H7N9, baculovirus, insect cells, ... Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay ... Characterization of cardiomyocytes derived from pluripotent cells often includes the analysis of reference markers, both at the ... Cell cultures are monitored for cytopathic effect from days 3-7 post-inoculation and any viruses grown in cell culture are ...
  https://www.jove.com/visualize/abstract/23922916/structural-functional-characterization-recombinant-death-domain-from
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cell culture. 50157. Play Button. Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells ... influenza, virus, vector, plasmid, cell, cell culture, PCR, sequencing ... and time to generate an air-liquid interface culture. Calu-3 cells, derived from a human bronchial adenocarcinoma, are an ... Cell Polarity, life sciences, Calu-3, polarized cell culture, epithelial cells, respiratory virus, liquid covered culture, ...
  https://www.jove.com/visualize/abstract/19816578/the-replicase-gene-avian-coronavirus-infectious-bronchitis-virus-is
*  MDCK - What does MDCK stand for? The Free Dictionary
MDCK and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation.. ... the influenza virus strain A/Pavia/07/2014 was recovered from the supernatant propagated in MDCK cell culture.. Swine influenza ... Antiviral activity of an aqueous extract derived from Aloe arborescens Mill, against a broad panel of viruses causing ... we see that viral vaccines are often made using a variety of cell lines including: Vero, MDCK, MRC 5, BHK and CHO.. Vaccine ...
  http://acronyms.thefreedictionary.com/MDCK
*  Cornelia L. Dekker, M.D. | Stanford Medicine Profiles
Safety and immunogenicity of inactivated, Vero cell culture-derived whole virus influenza A/H5N1 vaccine given alone or with ... Baseline Levels of Influenza-Specific CD4 Memory T-Cells Affect T-Cell Responses to Influenza Vaccines PLOS ONE He, X., Holmes ... We assessed the safety and immunogenicity of a Vero cell culture-grown inactivated whole virus influenza A/H5N1 vaccine with or ... cells in T and NK cell subsets, percentages of influenza-specific memory B-cells, HAI titer, age, and type of vaccine. The ...
  https://med.stanford.edu/profiles/cornelia-dekker
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
To obtain purified vRNPs, cell cultures are infected, and vRNPs are affinity purified from lysates derived from these cells. ... Infection, Issue 81, Influenza A virus, Orthomyxoviridae Infections, Influenza, Human, Influenza in Birds, Influenza Vaccines, ... Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells ... influenza, virus, vector, plasmid, cell, cell culture, PCR, sequencing ...
  https://www.jove.com/visualize/abstract/19462010/full-factorial-analysis-mammalian-avian-influenza-polymerase-subunits
*  Cell Type-Specific Recognition of Human Metapneumoviruses (HMPVs) by Retinoic Acid-Inducible Gene I (RIG-I) and TLR7 and Viral...
... and Vero cells were from the American Type Culture Collection (Manassas, VA). The human hepatocellular carcinoma cell lines ... heat-inactivated influenza (MOI = 0.1), or CpG-A (2 μM) for 24 h. Cells were preincubated with chloroquine (1 μM) or ... There is currently no effective treatment or vaccine for HMPV. Recent studies in mice have revealed an important role for T ... triphosphate moiety of viral genomes or virus-derived transcripts of negative-sense ssRNA viruses, whereas MDA-5 is activated ...
  http://www.jimmunol.org/content/184/3/1168.long
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay ... Seeding and culturing of permeable transwells with human derived lung epithelial cells is described, along with isolation of ... Vaccine efficacy studies currently require a large number of animals in order to be considered statistically significant and ... influenza virus hemagglutinin). ... Neural stem/precursor cells (NPCs) are a promising stem cell ...
  https://www.jove.com/visualize/abstract/23469095/risk-exposure-to-eastern-equine-encephalomyelitis-virus-increases
*  US Patent # 9,714,411. Animal protein-free media for cultivation of cells - Patents.com
... and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturin ... The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- ... Vero cell/vaccinia virus, Vero cell/hepatitis A virus, Vero cell/influenza virus, Vero cell/West Nile virus, Vero cell/SARS ... Cell cultures of recombinant mammalian cells (e.g. CHO-cells stably expressing Factor VIII=GD8/6-cells) were grown in ...
  http://patents.com/us-9714411.html
*  'influenza a virus' Protocols and Video...
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to ... Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells', 'Purification and Visualization of ... Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells', 'Multi-target ... Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells', 'Avian Influenza ...
  https://www.jove.com/keyword/influenza+a+virus
*  Yellow fever vaccine YF-17D activates multiple dendritic cell subsets via TLR2, 7, 8, and 9 to stimulate polyvalent immunity |...
T cells by FACS (A). The remaining cells were placed into culture and restimulated with SIINFEKL or cultured in media alone. ... but also cellular contaminants derived from the cell lines in which the vaccines were grown. Such cellular contaminants ( ... Viral titers were calculated by plaque assay using Vero cells. Virus was inactivated by either incubating at 56°C for 1 h or UV ... Involvement of toll-like receptor 3 in the immune response of lung epithelial cells to double-stranded RNA and influenza A ...
  http://jem.rupress.org/content/203/2/413
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Mammalian cell culture is the major platform for commercial production of human vaccines and therapeutic proteins. However, it ... Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells ... We investigated whether HCV propagation is suppressed by dominant restriction factors in either human cell lines derived from ... influenza virus hemagglutinin). ... Huh-7.5 cells). Subsequently, these cells were co-cultured and ...
  https://www.jove.com/visualize/abstract/22216177/replication-gene-expression-particle-production-consensus-merkel-cell