Trimerisation is important for the function of clathrin at the mitotic spindle | Journal of Cell Science
Cell biology. Human embryonic kidney HEK293 cells were cultured in DMEM containing 10% fetal bovine serum and 100 U/ml ... A) Representative confocal micrographs of each construct in HEK293 cells in metaphase. Bar, 10 μm. (B) A cell expressing 1-1639 ... Molecular biology. DNA plasmids to simultaneously knockdown endogenous human CHC by RNAi through expression of shRNA and to ... In the present study, we aimed to test these two hypotheses by replacing endogenous clathrin heavy chain (CHC) in human cells ...http://jcs.biologists.org/content/119/19/4071
Expression of Glycine-Activated Diheteromeric NR1/NR3 Receptors in Human Embryonic Kidney 293 Cells Is NR1 Splice Variant...
Maintenance of HEK293 Cells and Recombinant Receptor Expression.. In brief, HEK293 cells (American Type Culture Collection, ... Data represent the mean ± S.E.M. of 10 μM glycine steady-state current normalized to whole-cell capacitance (n = 3-11 cells). C ... Molecular Biology and Site-Directed Mutagenesis.. The cDNA clones of NR1 and NR3A used in these experiments were kindly ... Little current was found in human embryonic kidney 293 cells coexpressing either NR3A or NR3B and the NR1-1a splice variant. ...http://jpet.aspetjournals.org/content/331/3/975
HEK293 whole cell lysate (ab7902) | Abcam
HEK293 whole cell lysate Lysates datasheet (ab7902). Abcam offers quality products including antibodies, assays and other ... Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney and subsequently demonstrated to be ... Cell Biology. Epigenetics. Metabolism. Developmental Biology. By research area. Immunology. Microbiology. Neuroscience. Signal ... The positive control is HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902). Please refer to the Cell Lysate ...https://www.abcam.com/hek293-whole-cell-lysate-ab7902.html
HEK 293 cells - Wikipedia
... evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells ... "Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations". Nature Communications. 5 ... Human embryonic kidney cells 293, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a ... Fanelli, Alex (2016). "HEK293 Cell Line: human embryonic kidney cells". Retrieved 3 December 2017. Dautzenberg FM, Higelin J, ...https://en.wikipedia.org/wiki/HEK_293_cells
Most recent papers in the journal Cells, Tissues, Organs | Read by QxMD
HEK293 (human embryonic kidney 293) cells and their derived cell lines provide an attractive heterologous system for the ... Human Embryonic Kidney 293 Cells: A Vehicle for Biopharmaceutical Manufacturing, Structural Biology, and Electrophysiology. ... com/read/29393161/human-embryonic-kidney-293-cells-a-vehicle-for-biopharmaceutical-manufacturing-structural-biology-and- ... Mammalian cells, e.g., CHO, BHK, HEK293, HT-1080, and NS0 cells, represent important manufacturing platforms in bioengineering ...https://www.readbyqxmd.com/journal/34564
Frontiers | N-myristoylation of Antimicrobial Peptide CM4 Enhances Its Anticancer Activity by Interacting With Cell Membrane...
Despite increasing cytotoxicity against HEK293 and NIH3T3 cells and erythrocytes associated with its anticancer activity, ... Despite increasing cytotoxicity against HEK293 and NIH3T3 cells and erythrocytes associated with its anticancer activity, ... Accordingly, the membrane affinity towards breast cancer cells was two to three-fold higher than that of normal cells. ... Accordingly, the membrane affinity towards breast cancer cells was two to three-fold higher than that of normal cells. ...https://www.frontiersin.org/articles/10.3389/fphar.2018.01297/full
Direct interaction of eag domains and cyclic nucleotide-binding homology domains regulate deactivation gating in hERG channels ...
Human embryonic kidney 293 (HEK293) cell culture and transfection were performed as described previously (Gianulis and Trudeau ... HEK293 cells were plated on 35-mm cell culture dishes and transiently transfected with 1 µg hERG channel cDNA plus 1 µg N-eag- ... Molecular biology and cell culture. The hERG S4-S5 mutant constructs, including hERG Δeag[S4-S5]Alacomplete, were synthesized ... HEK293. human embryonic kidney 293. hERG. human eag-related gene. VSD. voltage-sensor domain. ...http://jgp.rupress.org/content/142/4/351
N-&-N, a new class of cell death-inducing kinase inhibitors derived from the purine roscovitine | Molecular Cancer Therapeutics
The HEK 293 human embryonic kidney and MDA-MB-231 human breast adenocarcinoma (kindly provided by Dr. Jan Mester) cell lines ... Cell Biology. Antibodies and Chemicals. AcDEVDafc and Q-VD-OPh were purchased from MPbiomedicals. Cell Titer 96 containing the ... N1 was not a specific property of SH-SY5Y cells, we tested (R)-roscovitine, N-&-N1, and N-&-N2 on seven human cell lines (Fig. ... Cell Death and Cell Viability Assessments. Cell viability was determined by measuring the reduction of MTS. Cell death was ...http://mct.aacrjournals.org/content/7/9/2713
Homo- and heteromeric assembly of TRPV channel subunits | Journal of Cell Science
Cell culture and transient transfection. Human embryonic kidney (HEK) 293 cells (ATCC, Manassas, VA) were maintained at 37°C ... Molecular biology. To obtain a pcDNA3-FLAG fusion vector, the FLAG (DYKDDDDK) epitope was introduced in the XbaI and ApaI sites ... was not evident in HEK293 cells. As TRPV2 is efficiently targeted to the plasma membrane in resting HEK293 cells, a secretion- ... Xu, F., Satoh, E. and Iijima, T. (2003). Protein kinase C-mediated Ca2+ entry in HEK 293 cells transiently expressing human ...http://jcs.biologists.org/content/118/5/917?ijkey=466cef738a247c18b459a04bcbb06d50c55ccb18&keytype2=tf_ipsecsha
Cell culture substrate "diXCOAT" | KISCO
HEK293(Human Embryonic Kidney 293). Sowing number：. 8.0×104 cells/well (24well). ... Inquire about bio development. 〒103-8410. 11-2, Nihonbashi Honcho 4-chome, Chuo-ku, Tokyo, 103-8410. 2nd Sales Division. Life ... Other cells in valuation process. *Mouse iPS [(Induced Pluripotent Stem) cell (differentiation promoter, maintaining ... Cell culture substrate "diXCOAT". Collaborating with the Tokyo University Industrial Technology center, KISCO developed ...http://www.kisco-net.com/business/chemicals/bio/dixcoat.php
HEK293 Cell Culture.. Human embryonic kidney (HEK) 293 fibroblasts (American Type Culture Collection, Manassas, VA) were ... The ability of CMPDA and CMPDB to potentiate glutamate-evoked influx of calcium into HEK293 cells stably expressing human GluA2 ... Molecular Biology.. Plasmids encoding cDNAs for the flip (i) and flop (o) variants of rat wild-type GluA2 were gifts of Dr. ... human embryonic kidney. FLIPR. fluorometric imaging plate reader. BAPTA. 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic ...http://molpharm.aspetjournals.org/content/80/2/267
Calcium homeostasis in human melanocytes: role of transient receptor potential melastatin 1 (TRPM1) and its regulation by...
Using heterologous expression in human embryonic kidney epithelial cells (HEK293), Xu et al. (42) showed that TRPM1 ... Applications of Advanced Cell Culture in Cell Physiology: Co-culture, Organoids, Stem Cells: Submission deadline December 31, ... Although a role for Ca2+ has been proposed in many aspects of melanocyte biology, the mechanisms that regulate Ca2+ entry and ... Modulation of normal human melanocyte dendricity by growth-promoting agents. Pigment Cell Res 6: 406-416, 1993. ...http://ajpcell.physiology.org/content/297/3/C679
Robert B. Glassman Memorial Brain, Mind, and Behavior Symposium | Neuroscience | Lake Forest College
Human Embryonic Kidney cells (HEK293) were used for the study due to past scientists' successes in overexpressing membrane ... Emily Fink, and K. Kam, Department of Cell Biology, Rosalind Franklin University of Medicine and Science, North Chicago, IL ... BJAB cells were used as KSHV-negative control cells and BCBL cells were used as KSHV-positive cell lines. Nucleolin was found ... Human mammary epithelial cells (HMEC) were used as non-IBC control cells. Inflammatory breast cancer cells (SUM149PT, SUM190PT ...http://www.lakeforest.edu/academics/programs/neuroscience/robert-b-glassman-memorial-brain-mind-and-behavior-symposium/
COS-7 cells (CRL-1651; American Type Culture Collection, Manassas, VA) and human embryonic kidney expressing T-antigen, clone ... Ten micrograms of cell lysate proteins were denatured by boiling in 2× Laemmli sample buffer (Bio-Rad, Hercules, CA) for 5 min ... HEK 293-T/17 cells were transfected with wild-type or mutated myc-GluR6a and enhanced green fluorescent protein cDNAs. Patch ... Cell culture and transfection.. Hippocampal neuronal cultures were prepared from 18-d-old embryonic Sprague Dawley rats as ...http://www.jneurosci.org/content/27/39/10423
Cell culture.. Stably transfected human embryonic kidney cells (HEK293 cells) and Madin-Darby canine kidney cells (MDCKII cells ... Cell Culture Multiwell Plate Cellstar; Greiner Bio-One) at an initial density of 700,000 cells per well. The cells were grown ... stably transfected HEK293 cells recombinantly expressing human OATP1B1 (HEK-OATP1B1), human OATP1B3 (HEK-OATP1B3) (5), or human ... HEK293 cell, human embryonic kidney cell. *IC50, half-maximal inhibitory concentration. *MDCKII cell, Madin-Darby canine kidney ...http://diabetes.diabetesjournals.org/content/57/6/1463
Divergent roles of a peripheral transmembrane segment in AMPA and NMDA receptors | JGP
Cell culture and transfection. Human embryonic kidney 293 (HEK 293) or HEK 293T cells were grown in Dulbecco's modified Eagle's ... Molecular biology and cell culture. All manipulations were made in rat GluN1 (GluN1a; NCBI Protein database accession no. ... To improve cell survivability, HEK 293 cells transfected with NMDAR subunits were bathed in a media containing the NMDAR ... Macroscopic currents in the whole-cell mode or outside-out patches, isolated from HEK 293 cells, were recorded at room ...http://jgp.rupress.org/content/149/6/661
Three Subfamilies of KRAB Zinc Finger Proteins : A Structural, Functional and Evolutionary Analysis
The effect of HKr19 on cell viability was investigated by transfection into human embryonic kidney cells (HEK 293). The results ... Department of Cell and Molecular Biology On the subject. Biological Sciences Search outside of DiVA. GoogleGoogle Scholar. ... 1999 In: DNA Cell Biol., Vol. 18, no 5, p. 381-396Article in journal (Refereed) Published Identifiers. urn:nbn:se:uu:diva-90632 ... 2001 In: DNA Cell Biol., Vol. 20, no 5, p. 275-286Article in journal (Refereed) Published Identifiers. urn:nbn:se:uu:diva-90633 ...http://www.diva-portal.org/smash/record.jsf?pid=diva2:163072
High mobility group box 1 protein interacts with multiple Toll-like receptors | Cell Physiology
HMGB1 increases TLR2- and TLR4-mediated NF-κB activation in human embryonic kidney (HEK)-293 cells. HEK-293 cells were ... Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG, goat anti-mouse IgG, and rabbit anti-goat IgG were from Bio-Rad ( ... Cells.. Murine macrophage RAW264.7 and human embryonic kidney (HEK)-293 cell lines were obtained from the American Type Culture ... Transient transfections into human embryonic kidney-293 cells showed that HMGB1 induced cellular activation and NF-κB-dependent ...http://ajpcell.physiology.org/content/290/3/C917
Gene Report for G00001379 - Genes2Cognition Neuroscience Research Programme
... cell culture [human embryonic kidney (HEK)-293 and immortalized rat renal proximal tubule cells] and biotinylation; (3) cell ... Systems and Cell Biology of Neurodegeneration, Department of Psychiatry Research, University of Zurich, Zurich, Switzerland. ... whereas flotillin-1 was most abundant in A498 kidney cells, muscle cell lines, and fibroblasts. Using three different models of ... D1 dopamine receptor signaling involves caveolin-2 in HEK-293 cells.. Yu P, Yang Z, Jones JE, Wang Z, Owens SA, Mueller SC, ...http://www.genes2cognition.org/db/Gene/G00001379
Diminished Adenosine A1 Receptor Expression in Pancreatic α-Cells May Contribute to the Pathology of Type 1 Diabetes | Diabetes
Cell transfection.. Human embryonic kidney (HEK) 293 cells were transfected with various plasmids alone or in combination using ... Cells were lysed. Protein concentrations were determined by Bradford assay (Bio-Rad), and cAMP levels were measured using the ... Measurement of cAMP levels in transfected HEK293 cells.. HEK293 cells (2.5 × 104) were grown to ∼80% confluency in 96-well ... As a control, cell lysates (input samples) were also immunoblotted for β-actin. cAMP accumulation was measured in HEK293 cells ...http://diabetes.diabetesjournals.org/content/62/12/4208
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
N-terminally FLAG epitope tagged Ino80 cDNA are stably introduced into human embryonic kidney (HEK) 293 cell lines using Flp- ... Developmental Biology, Issue 79, Eye, Photoreceptor Cells, Genes, Developmental, neuron, visualization, degeneration, ... Molecular Biology, Issue 52, GM-CSF, GM-CSF autoantibody, GM-CSF receptor α, receptor binding assay, cell free system ... In the event an individual subunit is to be depleted, one transfects siRNAs targeting this subunit into an HEK 293 cell line ...https://www.jove.com/visualize/abstract/22768245/flp-recombinase-mediated-site-specific-recombination-silkworm-bombyx
A STIM1-dependent 'trafficking trap' mechanism regulates Orai1 plasma membrane residence and Ca2+ influx levels | Journal of...
Cell culture. CHO cells were obtained from the ATCC (CCL-61) and human embryonic kidney (HEK293) cells were cultured at 37°C ... Our fourth Special Issue will focus on the cell biology of the immune system, guest edited by Ana-Maria Lennon-Duménil. In this ... Cells were then released to 37°C and cell-associated anti-HA measured (Fig. 4E). With Ca2+ stores full, Orai1 showed the ... Cells were then released to 37°C and cell associated anti-HA measured. Data are the mean±s.e.m. of three independent ...http://jcs.biologists.org/content/128/16/3143
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... by day 10 across all human embryonic stem cell (hESC) and hiPSC lines tested to date. Cells can be passaged and maintained for ... Cellular Biology, Issue 90, zebrafish; kidney; nephron; nephrology; renal; regeneration; proximal tubule; distal tubule; ... Here the protocols for culturing the medium spiny neurons and generating HEK293 cells lines expressing GABAARs are first ... Stem Cell Biology, Issue 63, Immunology, T cells, induced pluripotent stem cells, differentiation, Notch signaling, T cell ...https://www.jove.com/visualize/abstract/24465914/identification-novel-thymic-epithelial-cell-subsets-whose
The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of...
ethanolic extract (OSE) on human embryonic kidney-293 (HEK-293) cells as the in vitro model of neurodegenerative diseases ... significantly increased the viability of TMT-induced apoptotic cells and maintained cell viability of the normal HEK-293 cells ... Nastiti Wijayanti: Department of Animal Physiology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia. ... ethanolic extract (OSE) on human embryonic kidney-293 (HEK-293) cells as the in vitro model of neurodegenerative diseases. ...http://www.veterinaryworld.org/Vol.11/September-2018/6.html
Advancing 3D Cell Culture for Biomedical Research Using Primary Cells
... primary cells may be the better choice for cell culture, especially 3D culture meant to faithfully model donor tissue. ... Although cell lines are the familiar choice, says Lonza, ... cells by human adenoviruses and the origin of HEK 293 cells. ... cells had been changed by adenovirus transformation so they more closely resembled immature neurons than embryonic kidney cells ... including cancer biology and drug discovery. The cell types available for use in cell culture are either cell lines or primary ...https://www.genengnews.com/magazine/advancing-3d-cell-culture-for-biomedical-research-using-primary-cells/