Hydrogel cell patterning incorporating photocaged RGDS peptides | SpringerLink
As we aim towards enhancing our knowledge of complex cell behaviors and developing intricate cell-based devices and improved ... Here we have developed a novel strategy to pattern cells using a hyaluronic acid hydrogel material and photocaged RGDS (Arg-Gly ... We further demonstrate the ability of this modified hydrogel material to pattern fibroblast cells on the micron scale using ... light exposure through a patterned photomask to selectively switch areas of the hydrogel surface from cell non-adhesive to cell ...https://link.springer.com/article/10.1007/s10544-010-9412-7
Polymers | Free Full-Text | Microscale Strategies for Generating Cell-Encapsulating Hydrogels
Microfabrication-based techniques are emerging as powerful approaches to generate such cell-encapsulating hydrogel structures. ... we introduce common hydrogels and their crosslinking methods and review the latest microscale approaches for generation of cell ... These gels provide a structure inside which cells can spread and proliferate. Such structures benefit from controlled ... Hydrogels in which cells are encapsulated are of great potential interest for tissue engineering applications. ...http://www.mdpi.com/2073-4360/4/3/1554
Substrate induced differentiation of human mesenchymal stem cells on hydrogels with modified surface chemistry and controlled...
Polyacrylamide hydrogels were prepared with variable stiffness within a range of effective surface Young's modulus values from ... stem cells. on hydrogels with modified surface chemistry and controlled modulus Mathieu Lanniel,a Ejaz Huq,b Stephanie Allen,a ... stem cells. on hydrogels with modified surface chemistry and controlled modulus M. Lanniel, E. Huq, S. Allen, L. Buttery, P. M ... cell. densities achieved for collagen. coated gels. The spread of each cell. was shown to be greater for the stiffer surfaces ...http://pubs.rsc.org/en/Content/ArticleLanding/2011/SM/c1sm05167a
Rheological Properties of Cell-Hydrogel Composites Extruding Through Small-Diameter Tips | Journal of Manufacturing Science and...
... the cell-hydrogel composites (with the maximum cell concentration of 10. 7. ∕. ml. ) show no difference from hydrogel materials ... So, the design of cell extrusion nozzle turns into an important phase, and the rheological properties of cell-hydrogel ... Based on these, the manufacturability of cell-hydrogel composites was discussed. Also, the cell viability, which is another ... Rheological Properties of Cell-Hydrogel Composites Extruding Through Small-Diameter Tips Jie Cheng, Feng Lin, Haixia Liu, ...http://manufacturingscience.asmedigitalcollection.asme.org/article.aspx?articleid=1452029
talks.cam : Bioengineering human liver organoids using induced-pluripotent stem cells in 3D hydrogel
Bioengineering human liver organoids using induced-pluripotent stem cells in 3D hydrogel. Add to your list(s) Download to your ... Bioengineering human liver organoids using induced-pluripotent stem cells in 3D hydrogel ... Generation of human organoids from induced pluripotent stem cells (iPSCs) offers exciting possibilities for developmental ... immortalized cell lines and resultant structures that are difficult to control or scale. To address these challenges, we aimed ...http://talks.cam.ac.uk/talk/index/104047
Nanoscale Hydrogel Coatings on Cells | www.bme.ufl.edu
Purities ,95% are common when sorting mixtures of cultured cells and for isolation of cells from whole blood. Multiple modes of ... Critically, the system is designed for high purity, where all uncoated cells are readily lysed. ... that is based on the selective deposition of a protective coating on a cell and the destruction of all unprotected cells. This ... and his work has found a natural fit in the modification of living cell and tissue interfaces. He earned his B.S. in Chemical ...http://www.bme.ufl.edu/node/1701
DSpace@MIT: Bioengineered surfaces and hydrogels for specific cell capture and release from whole blood
Bioengineered surfaces and hydrogels for specific cell capture and release from whole blood. Research and Teaching Output of ... Bioengineered surfaces and hydrogels for specific cell capture and release from whole blood. Download ... Microfluidic affinity-based cell capture devices are presently able to isolate specific cell populations from heterogeneous ... The captured cells may be released by applying a gentle chelating buffer which dissolves the gel, eliminating both the specific ...http://dspace.mit.edu/handle/1721.1/45953
Development of thermally-crosslinked hydrogels as injectable cell carriers for orthopaedic tissue engineering
... ... These results demonstrate the great potential of OPF hydrogels as injectable carriers for delivery of cells to a variety of ... Before their use as injectable cell carriers, the cytotoxicity of all OPF hydrogel precursor molecules, including radical ... was evaluated using rat marrow stromal cells as a model cell type. Results indicated that the overall pH of the formulation, as ...https://scholarship.rice.edu/handle/1911/18714
Attacking Cancer Cells with Hydrogel Nanoparticles
... into cancer cells. Once in the cell the siRNA turns on the programmed cell death the body uses to kill mutated cells and help ... Cells use the messenger RNA (mRNA) to generate proteins, which help to keep the cell growing. Once the siRNA enters the cell, ... The hydrogel releases only a small amount of siRNA at a time, ensuring that while some are out in the cancer cell doing their ... "We've decorated the surface of these hydrogels with a ligand that tricks the cancer cell into taking it up. Once inside, the ...http://bestinscience.com/nanotechnology/medical/431-attacking-cancer-cells-with-hydrogel-nanoparticles
Human neuroblastoma (SH-SY5Y) cell culture and differentiation in 3-D collagen hydrogels for cell-based biosensing [electronic...
We have used SH-SY5Y neuroblastoma cells cultured in 3-D collagen hydrogel as a cell-based biosensor. There was no ... Human neuroblastoma (SH-SY5Y) cell culture and differentiation in 3-D collagen hydrogels for cell-based biosensing [electronic ... 3-D cells were less responsive in comparison to 2-D cells, supporting the hypothesis that 2-D cell calcium dynamics may be ... Cell-based three-dimensional systems are desirable in the field of high throughput screening assays due to their similarity to ...http://www.openthesis.org/documents/Human-neuroblastoma-SH-SY5Y-cell-401872.html
Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects - Oliveira - 2010...
Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects. ... Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects. J. Orthop. Res ... Gellan gum injectable hydrogels for cartilage tissue engineering applications: in vitro studies and preliminary in vivo ... A novel injectable polysaccharide hydrogel: modified gellan gum for long-term cartilage regeneration in vitro. J Mater Chem 19 ...http://onlinelibrary.wiley.com/doi/10.1002/jor.21114/references
Biomaterials: engineering cell niches hydrogels - University of Manchester | Coursera
Differentiation of Mouse Embryonic Stem Cells Using PEG-Fibrinogen Hydrogel Microspheres
... based hydrogels with a conjugated fibrinogen protein functional motif. Hydrogels consisted of dispersed microsphere cell- ... Differentiation of Mouse Embryonic Stem Cells Using PEG-Fibrinogen Hydrogel Microspheres. View/. Open. Thesis_full_document ( ... Cells were encapsulated within the hydrogel polymer precursor and microspheres were generated via water-in-oil emulsification. ... Differentiation of encapsulated cells was verified using qPCR and immunohistochemical techniques. This method is the first to ...https://etd.auburn.edu/handle/10415/3145
3D cell culture in wood-based nanocellulose hydrogel - Wikipedia
NFC hydrogel in 3D cell culture offers a platform for various biomedical applications. Different cell lines and cell types have ... like cell culturing. GrowDex is NFC hydrogel for 3D cell culture commercialized by UPM, Finland. NFC fiber network structure ... Hydrogel from wood-based nanofibrillated cellulose (NFC) is used as a matrix for 3D cell culture. As plant based material, it ... The nanocellulose hydrogel can be completely degraded by cellulase enzyme treatment while retaining the 3D cell structures. ...https://en.wikipedia.org/wiki/3D_cell_culture_in_wood-based_nanocellulose_hydrogel
Micromachines | Free Full-Text | Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate...
... we have successfully demonstrated to fabricate two layers of the cell-encapsulated alginate hydrogels, where cells in each ... we first successfully produced cell-encapsulated multilayer alginate hydrogels with different shapes and sizes in each layer ... for three-dimensional cell patterning. In this system, the magnetically-labeled cells were first manipulated into a specific ... Our proposed method provides a programmable method for the spatiotemporally controllable assembly of cell populations into ...http://www.mdpi.com/2072-666X/5/4/1173
Hydrogels with tunable stress relaxation regulate stem cell fate and activity. - PubMed - NCBI
Time to Relax: Mechanical Stress Release Guides Stem Cell Responses. [Cell Stem Cell. 2016] ... Hydrogels with tunable stress relaxation regulate stem cell fate and activity.. Chaudhuri O1,2,3, Gu L1,2, Klumpers D1,2,4, ... independently of the hydrogel's initial elastic modulus, degradation, and cell-adhesion-ligand density. We find that cell ... 3 um of cell border relative to the background of the hydrogel. Data are shown as mean +/− s.d. and **** indicates p , 0.0001 ( ...https://www.ncbi.nlm.nih.gov/pubmed/26618884?access_num=26618884&link_type=MED&dopt=Abstract
Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype - Biomaterials Science (RSC...
... was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 ... A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N′-methylenebisacrylamide, which ... Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype C. Mangani, A. Lilienkampf, M ... A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N′-methylenebisacrylamide, which ...http://pubs.rsc.org/en/content/articlelanding/2015/bm/c5bm00121h
Cells behave distinctly within sponges and hydrogels due to differences of internal structure.
... hydrogels, and nanofibers, have been broadly used in cartilage regeneration; however, effects of internal structures of the ... biomaterials on cells and chondrogenesis remain largely unex ... Cells behave distinctly within sponges and hydrogels due to ... Cells, Cultured. Chondrocytes / cytology*, physiology. Hydrogels / chemistry*. Porifera / chemistry*. Real-Time Polymerase ... We hypothesized that different internal structures of sponges and hydrogels led to phenotypic disparity of the cells and may ...http://www.biomedsearch.com/nih/Cells-Behave-Distinctly-within-Sponges/23614637.html
Hydrogel showed 'staggering' results in diabetic cell study - McKnight's Long Term Care News
A new hydrogel created by engineering researchers at the University of Toronto prompts skin cells to crawl toward one another, ... A new hydrogel created by engineering researchers at the University of Toronto prompts skin cells to crawl toward one another, ... Hydrogel showed 'staggering' results in diabetic cell study. Share this content: *facebook ... A team led by Professor Milica Radisic, Ph.D., tested the peptide-hydrogel biomaterial on healthy human skin cells, called ...http://www.mcknights.com/news/hydrogel-showed-staggering-results-in-diabetic-cell-study/article/635863/
Evaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems - Biomaterials Science (RSC...
... in cell therapy appears as a powerful tool for the treatment of central nervous system diseases, including traumatic brain and ... Evaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems E. Mauri, A. Sacchetti, N. ... Evaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems ... The introduction of the tripeptide, through hydrogel chemical functionalization, improved the percentage of proliferating cells ...http://pubs.rsc.org/en/content/articlelanding/2018/bm/c7bm01056g
Assessment of Lemon Balm (Melissa officinalis L.) Hydrogels: Quality and Bioactivity in Skin Cells
Cell Lines and Cell Culture. Human keratinocyte cell line HaCaT was purchased from the Cell Lines Service GmbH (Germany). Cells ... Cell viability-decreasing H2O2 concentrations determined in our experiments with HaCaT cells (EC50 ) were similar to those ... cells with the investigated preparations were transferred to 96-well plates at concentrations of 30,000 cells/well. The cells ... Assessment of Lemon Balm (Melissa officinalis L.) Hydrogels: Quality and Bioactivity in Skin Cells. Kristina Ramanauskienė,1 ...https://www.hindawi.com/journals/ecam/2015/635975/
'Hydrogels' boost ability of stem cells to restore eyesight and heal brains - Healthcanal.com : Healthcanal...
In addition to examining how the stem cells benefit from life in hydrogels, the researchers also showed that these new cells ... One part of the Stem Cell Reports study involved the team injecting hydrogel-encapsulated photoreceptors, grown from stem cells ... showing that the hydrogels do more than just hold stem cells together; they directly promote stem cell survival and integration ... Because the hydrogel could boost cell survival in two different parts of the nervous system, the eye and the brain, it could ...https://www.healthcanal.com/brain-nerves/63489-hydrogels-boost-ability-of-stem-cells-to-restore-eyesight-and-heal-brains.html
Self-healing cell wall particles hydrogels: a rheological investigation | Archivio Istituzionale della Ricerca
Cell wall materials, or some selected constitutive components, might hence play a key role as adaptable thickening agent in ... Plant and fruit cells encase themselves within a complex polysaccharide wall which are complex and highly sophisticated ... In this work the viscoelastic properties of cell wall particles dispersions, which were extracted from fresh and aged apple ... This means that the cell wall particle dispersions have self-healing capabilities. They can be easily processed as low ...https://air.unimi.it/handle/2434/354469
Fabrication of Magnetic Poly (ethylene glycol) Hydrogels Blocks Using Microfluidic System for Cell Patterning Techniques
... we developed a novel technique called magnetic hydrogel-based cell patterning (MHCP) to easily achieve heterotypic cell ... We also demonstrated that the material of magnetic hydrogel and the processes of MHCP produced negligible effects on cell ... The 2-D heterotypic cell patterning and layer-by-layer 3-D tissue reconstruction could be applied as more complex models in ... we developed a novel technique called magnetic hydrogel-based cell patterning (MHCP) to easily achieve heterotypic cell ...http://www.techconnectworld.com/Microtech2011/a.html?i=1519
Skin cells 'crawl' together to heal wounds treated with unique hydrogel layer | EurekAlert! Science News
A team led by Professor Milica Radisic in U of T Engineering has demonstrated for the first time that their peptide-hydrogel ... biomaterial prompts skin cells to 'crawl' toward one another, closing chronic, non-healing wounds often associated with ... They knew it promoted survival of many different cell types, including stem cells, heart cells and fibroblasts (the cells that ... Skin cells 'crawl' together to heal wounds treated with unique hydrogel layer Research team led by Milica Radisic uses their ...https://www.eurekalert.org/pub_releases/2016-12/uotf-sc121216.php