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*  Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High...
The genotyping technique described here, which couples fluorescent polymerase chain reaction (PCR) to capillary gel ... For example, the expression of a particular gene can be attenuated by gene knockdown1,2 (partial reduction) or gene knockout3,4 ... it is extremely useful in genotyping multi-allelic cells, such as cancer cells that have aneuploidy or genetic amplifications. ... Current genotyping techniques either suffer from inherent limitations or incur high cost, hence rendering them unsuitable for ...
  https://www.jove.com/video/55586/using-fluorescent-pcr-capillary-gel-electrophoresis-technique-to
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Gene Transfer Techniques, Transduction, Genetic, Transfection, Adenovirus, gene transfer, microRNA, overexpression, knock down ... The ERs function as transcription factors and regulate gene expression. Whereas ERα's regulation of protein-coding genes is ... Primary T cells can be manipulated through ectopic gene expression, but common methods fail to target the most important naive ... The RNA extraction technique we describe was selected to allow maximal recovery of very small RNA for use in a miRNA microarray ...
  https://www.jove.com/visualize/abstract/25932650/tmrec-database-transcription-factor-mirna-regulatory-cascades-human
*  RNA interference - Wikipedia
Since RNAi may not totally abolish expression of the gene, this technique is sometimes referred as a "knockdown", to ... a form of innate immunity that reacts nonspecifically to foreign genetic material. Mouse oocytes and cells from early mouse ... RNA sequences (siRNA and miRNA) that are complementary to parts of a promoter can increase gene transcription, a phenomenon ... Such techniques take advantage of the stable and heritable RNAi phenotype in plant stocks. Cotton seeds are rich in dietary ...
  https://en.wikipedia.org/wiki/RNA_interference
*  Glossary of gene expression terms - Wikipedia
Regulation of gene expression - the cell's system for controlling the expression of genes. Replication - a technique to ... Gene knockdown - refers to techniques by which the expression of one or more of an organism's genes is reduced, either through ... a National Center for Biotechnology Information database for gene expression Gene knockout - is a genetic technique in which an ... and enhances transcription from nearby promoter. Baseline - a measure of the gene expression level of a gene or genes prior to ...
  https://en.wikipedia.org/wiki/Glossary_of_gene_expression_terms
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Gene Transfer Techniques, Transduction, Genetic, Transfection, Adenovirus, gene transfer, microRNA, overexpression, knock down ... Extension of the technique to living cells is also described.. Basic Protocol, Issue 82, Microscopy, Super-resolution imaging, ... The ERs function as transcription factors and regulate gene expression. Whereas ERα's regulation of protein-coding genes is ... Primary T cells can be manipulated through ectopic gene expression, but common methods fail to target the most important naive ...
  https://www.jove.com/visualize/abstract/23342057/ack1-activation-and-regulation-by-allostery
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Gene Transfer Techniques, Transduction, Genetic, Transfection, Adenovirus, gene transfer, microRNA, overexpression, knock down ... The ERs function as transcription factors and regulate gene expression. Whereas ERα's regulation of protein-coding genes is ... Cellular Biology, Issue 92, Embryonic stem cell, Embryoid body, Hematopoietic Progenitor Cells, Retrovirus, Gene Expression, ... The RNA extraction technique we describe was selected to allow maximal recovery of very small RNA for use in a miRNA microarray ...
  https://www.jove.com/visualize/abstract/19844573/microrna-mir-378-regulates-nephronectin-expression-modulating
*  51
Systematic knockdown of thebaine 6-O-demethylase gene in Papaver somniferum L., using virus-induced gene silencing technique ( ... Taxol prevents the depolymerization of microtubules and inhibits cell division in tumor tissues. JAMYC is a transcription ... Biometric parameters were converted to genetic parameters using nested mating design model، by which genetic variance ... Therefore، tissue culture techniques are proposed for propagation and conservation purposes of the species. Usual regeneration ...
  http://www.magiran.com/magtoc.asp?mgID=3502&Number=51&Appendix=0&l=en
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
These techniques can be used to analyze terminal cells in wild-type and mutant animals, or genetic mosaics. Because of the high ... The pair-rule gene, hairy, encodes a basic helix-loop-helix transcription factor and is required for patterning of the early ... This technique has been widely exploited in tissue culture cells, and to a lesser extent, in primary tissue. The application of ... Here, we described a detailed method of dsRNA injection into Drosophila embryo to knockdown individual gene expression. We ...
  https://www.jove.com/visualize/abstract/21152432/the-bhlh-transcription-factor-hairy-refines-terminal-cell-fate
*  7 - Gene Manipulation II Flashcards by Kevin Bradley | Brainscape
Delivering transcription factors to adult stells to produce Induced pluripotent stem cells (iPS cells). - Inserting Sox2 into ... What is the TetOff and TetOn technique? What is the advantages and disadvantages of these techniques? ... Morpholino oligos can knock down gene expression, modify RNA splicing or inhibit miRNA activity and maturation ... transposons are highly compatible at transducing genetic material, making them ideal genetic tools. ...
  https://www.brainscape.com/flashcards/7-gene-manipulation-ii-1754676/packs/3196278
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Gene Transfer Techniques, Transduction, Genetic, Transfection, Adenovirus, gene transfer, microRNA, overexpression, knock down ... Pro-IL-1β protein expression is limited in resting cells; therefore a priming signal is required for IL-1β transcription and ... The results of this particular whole blood assay demonstrate a novel technique useful in elucidating patient cohorts presenting ... Subsequently, the infected cells are seeded on coverslips to finally enable live cell imaging. Then, living cells are incubated ...
  https://www.jove.com/visualize/abstract/20174657/overexpression-endoplasmic-reticulum-chaperone-bip3-regulates-xa21
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Gene Knockdown Techniques, C. elegans, dsRNA interference, gene knockdown, large scale feeding screen ... technique does not require the time-consuming establishment of stably transfected cell lines in which the transfected genetic ... Developmental Biology, Issue 38, Drosophila, Imaginal discs, Laser microdissection, Gene expression, Transcription profiling, ... Thus, to analyze cell-specific gene expression profiles from Drosophila tissues, it may be necessary to isolate specific cell ...
  https://www.jove.com/visualize/abstract/23056495/anion-sensitive-fluorophore-identifies-drosophila-swell-activated
*  Site-Directed Genome Knockout in Chicken Cell Line and Embryos Can Use CRISPR/Cas Gene Editing Technology | G3: Genes | Genomes...
For that reason, we evaluated the gene knock-down efficiency of this technique in somatic founder cells, nonfounder ESCs, and ... HEK293 cells, induced pluripotent stem cells) and knockout animals (mouse, rat, and zebrafish) using microinjection techniques ... H. Wang et al. (2013) generated a mouse model with disruptions and insertions in the Sry and Uty genes using transcription ... 1989 Genetic and structural characterization of the avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria. Mol. ...
  http://www.g3journal.org/content/6/6/1787
*  Perturb-seq - Wikipedia
Inferring a gene's function by applying genetic perturbations to knock down or knock out a gene and studying the resulting ... Once cells have been isolated at the single cell level, reverse transcription, amplification and sequencing takes place to ... Droplet-based microfluidics platforms (or other cell sorting and separating techniques) are used to isolate individual cells, ... performed a proof of concept experiment by using the technique to probe regulatory pathways related to innate immunity in mice ...
  https://en.wikipedia.org/wiki/Perturb-seq
*  CRISPR interference - Wikipedia
In prokaryotes, this steric inhibition can repress transcription of the target gene by almost 99.9%. Whereas in human cells, up ... is a genetic perturbation technique that allows for sequence-specific repression or activation of gene expression in ... For bacteria, gene knockdown by CRISPRi has been fully implemented and characterized (off-target analysis, leaky repression) ... with comparable efficiency to existing techniques such as RNAi and TALE proteins. In tandem or as its own system, CRISPRi could ...
  https://en.wikipedia.org/wiki/CRISPR_interference
*  Induced stem cells - Wikipedia
CD47 knockdown acutely increases mRNA levels of c-Myc and other stem cell transcription factors in cells in vitro and in vivo. ... human iPS cell-derived myeloid cell lines as unlimited cell source for dendritic cell-like antigen-presenting cells". Gene ... Fusion of somatic cells with pluripotent stem cells and Transformation of somatic cells into stem cells, using the genetic ... The technique can be applied to a large number of cells to produce stem cells for medical purposes on an industrial scale. ...
  https://en.wikipedia.org/wiki/Induced_stem_cells
*  Gene silencing - Wikipedia
It is the first approved food product to use that technique. CRISPR DNA-directed RNA interference Gene drive Gene knockdown ... In addition, siRNA techniques used in hepatitis C were able to lower the amount of the virus in the cell by 98%. RNA ... Gene silencing can occur during either transcription or translation and is often used in research. In particular, methods used ... Phylactou, L. (1 September 1998). "Ribozymes as therapeutic tools for genetic disease". Human Molecular Genetics. 7 (10): 1649- ...
  https://en.wikipedia.org/wiki/Gene_silencing
*  Zebrafish - Wikipedia
The morpholino is able to integrate into most cells of the embryo. A known problem with gene knockdowns is that, because the ... Because this approach would aim to target the "identity" of the melanoma cell rather than a single genetic mutation, ... A common reverse genetics technique is to reduce gene expression or modify splicing using Morpholino antisense technology. ... In 1999, the nacre mutation was identified in the zebrafish ortholog of the mammalian MITF transcription factor. Mutations in ...
  https://en.wikipedia.org/wiki/Zebrafish
*  DNA methylation - Wikipedia
When located in a gene promoter, DNA methylation typically acts to repress gene transcription. DNA methylation is essential for ... Hypomethylation of DNA affects gene that alter smooth muscle cell proliferation, cause endothelial cell dysfunction, and ... "RNA interference knockdown of DNA methyl-transferase 3 affects gene alternative splicing in the honey bee". PNAS. 110: 12750- ... Methyl Sensitive Southern Blotting is similar to the HELP assay, although uses Southern blotting techniques to probe gene- ...
  https://en.wikipedia.org/wiki/DNA_methylation