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*  Patente US5538871 - In situ polymerase chain reaction - Google Patentes
... a process of in vitro enzymatic amplification of specific nucleic acid sequences within the cells where they originate, can be ... Reaction initiation is delayed until the start of PCR thermal cycling, either by withholding a subset of PCR reagents from the ... or by adding to the PCR reagents a single-stranded DNA binding protein which blocks reaction at temperatures below about 50 C. ... achieved by changing the way that the enzymatic reaction is started. ...
  http://www.google.es/patents/US5538871
*  CiNii 論文 - Extrarenal rhabdoid tumors of soft tissue : Clinicopathological and molecular...
Gene expression of malignant rhabdoid tumor cell lines by reverse transcriptase-polymerase chain reaction SUZUKI A ... Detection of SYT-SSX fusion transcripts in synovial sarcoma by reverse transcription-polymerase chain reaction using archival ... Interphase fluorescence in situ hybridization and reverse transcription polymerase chain reaction as diagnostic aid for ... Mutations of the p53 gene in malignant rhabdoid tumors of soft tissue and the kidney : Immunohistochemical and DNA direct ...
  https://ci.nii.ac.jp/naid/10018195551
*  Enhanced Lymph Vessel Density, Remodeling, and Inflammation Are Reflected by Gene Expression Signatures in Dermal Lymphatic...
Quantitative reverse transcriptase-polymerase chain reaction.. One microgram of amplified total RNA was transcribed into cDNA ... Tissue inflammation modulates gene expression of lymphatic endothelial cells and dendritic cell migration in a stimulus- ... After 3 h, nonadherent cells were washed away, and adherent THP-1 cells were photographed with an inverted live cell microscope ... we explored immune cell abundance in the same skin samples used for LEC isolation. Although T-cell and dendritic cell counts ...
  http://diabetes.diabetesjournals.org/content/62/7/2509.full
*  Isolation from a human MDR lung cell line of multiple clonal subpopulations which exhibit significantly different drug...
Alterations in expression of the MDR-associated genes MDR1 and Topo IIalpha were observed, with no detectable changes in the ... The clonal cell lines exhibit a wide range of resistance extents, with the most resistant clone displaying 9 times the extent ... However, each clonal cell line displayed a distinct extent of expression of MDR1 and Topo IIalpha and further characterisation ... The DLKP-A cell line exhibits resistance to the classical MDR drugs, overexpresses P-glycoprotein and displays reduced ...
  https://www.semanticscholar.org/paper/Isolation-from-a-human-MDR-lung-cell-line-of-clonal-Heenan-O%27Driscoll/58ddf26d364078dfd14a468e63873726998e979e
*  Michael F. Clarke, M.D. | Stanford Medicine Profiles
We performed multiplexed single-cell gene expression analysis with quantitative reverse transcriptase polymerase chain reaction ... Cells were analyzed by real-time quantitative reverse transcription polymerase chain reaction, single-cell gene expression ... The DR alpha genes in both cell lines are hypermethylated relative to the same genes in T-cell lines infected with human T-cell ... A gene expression analysis revealed that the expression of stem cell associated genes, cell survival genes, transcription ...
  https://med.stanford.edu/profiles/michael-clarke
*  Philip Beachy | Stanford Medicine Profiles
We performed multiplexed single-cell gene expression analysis with quantitative reverse transcriptase polymerase chain reaction ... The cancer stem cell hypothesis suggests that malignant growth depends on a subset of tumor cells with stem cell-like ... Cancer is increasingly being viewed as a stem cell disease, both in its propagation by a minority of cells with stem-cell-like ... We demonstrate that these basal cells include stem cells capable of regenerating all cell types within the urothelium, and are ...
  https://med.stanford.edu/profiles/philip-beachy
*  B-cell translocation gene 1 is downregulated by promoter methylation in ovarian carcinoma
Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Total RNA was isolated using TRI Reagent (Molecular ... B-cell translocation gene 1 (BTG1) was originally identified as a translocation partner of the c-myc gene in B-cell chronic ... B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle ... OVCAR3 cell lines using methylation-specific polymerase chain reaction (MSP), indicating that promoter methylation may be a ...
  http://www.jcancer.org/v08p2669.htm
*  Analysis of losses of heterozygosity of the candidate tumour suppressor gene DMBT1 in melanoma resection specimens.
... a candidate tumour suppressor gene located on chromosome 10q25.3-q26.1, has recently been identified and found to be deleted in ... Receptors, Cell Surface / genetics*. Reverse Transcriptase Polymerase Chain Reaction. Skin Neoplasms / genetics, pathology, ... Applying reverse-transcription polymerase chain reaction (RT-PCR), DMBT1 transcription was not found in melanomas. However, ... whereas the naevi and melanoma cells themselves were negative. All considered, the candidate tumour suppressor gene DMBT1 does ...
  http://www.biomedsearch.com/nih/Analysis-losses-heterozygosity-candidate-tumour/12239452.html
*  Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar...
... two-step single-cell reverse transcriptase-polymerase chain reaction technique for analyzing gene expression at the single-cell ... fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and ... fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and ... fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and ...
  http://cadair.aber.ac.uk/dspace/handle/2160/1474
*  A Four-Gene Expression Signature for Prostate Cancer Cells Consisting of UAP1, PDLIM5, IMPDH2, and HSPD1
... reverse transcriptase polymerase chain reaction) assays were developed and validated. A mathematical formula based on the gene ... Development and Assessment of the 4-Gene RT-PCR Test. The 4-gene molecular assay was developed using the gene expression values ... Five genes were found to be acceptable for use as reference genes for quantitative gene expression analysis. All 5 reference ... A Four-Gene Expression Signature for Prostate Cancer Cells Consisting of UAP1, PDLIM5, IMPDH2, and HSPD1 .utlogo1 { display: ...
  https://www.urotoday.com/volume-2-2009/vol-2-issue-4/30455-a-fourgene-expression-signature-for-prostate-cancer-cells-consisting-of-uap1-pdlim5-impdh2-and-hspd1-2223371.html
*  Induction of the Cytochrome P450 Gene CYP26 during Mucous Cell Differentiation of Normal Human Tracheobronchial Epithelial...
A 0.4-base pair cDNA probe for human CYP26 was obtained by reverse transcriptase polymerase chain reaction (RT-PCR) using two ... B, induction of mucin gene expression by 4-hydroxy-RA (4-OH-RA) and TTAB in HTBE cells. HTBE cells were treated with 1 μM 4-OH- ... CYP26 mRNA Expression Is Not Induced in Many Lung Carcinoma Cell Lines.. In contrast to HTBE cells, lung carcinoma cell lines ... 1991) Expression of nuclear retinoic acid receptors in normal tracheobronchial cells and in lung carcinoma cells. Exp Cell Res ...
  http://molpharm.aspetjournals.org/content/58/3/483
*  HKU Scholars Hub: Preferential type1-1 cytokine gene expressions in peripheral T-cell lymphomas
Reverse Transcriptase Polymerase Chain Reaction. en_HK. dc.subject.mesh. T-Lymphocytes - metabolism. en_HK. ... Article: Preferential type1-1 cytokine gene expressions in peripheral T-cell lymphomas. *Show simple item record ... Preferential type1-1 cytokine gene expressions in peripheral T-cell lymphomas. en_HK. ... we have investigated whether a pattern of cytokine gene expression can be found in non-Hodgkin's peripheral T-cell lymphoma ( ...
  http://hub.hku.hk/handle/10722/78016
*  The Witebsky Center :: Kate Rittenhouse-Olson
These cell lines were analyzed by Glyco-gene chip array, real-time quantitative reverse transcriptase polymerase chain reaction ... Differential mRNA expression and flow cytometric analysis of cell surface expression of different carbohydrate genes and ... The Glyco-gene chip array allows for analysis of 1800 genes related to either carbohydrate antigen production or carbohydrate ... Ongoing studies are attempting to further understand the anti- metastatic role of antibody to TF-Ag by using cell lines which ...
  http://www.smbs.buffalo.edu/wcmpi/Faculty/rittenhouseolson.html
*  DDT and its metabolites alter gene expression in human uterine cell lines through estrogen receptor-independent mechanisms. -...
DDT and its metabolites alter gene expression in human uterine cell lines through estrogen receptor-independent mechanisms. by ... Reverse-transcriptase polymerase chain reaction (RT-PCR) analysis was performed as previously described (62). Briefly, RNA was ... Cell culture, transient transfection, and reporter gene assay. Ishikawa endometrial adenocarcinoma cells were grown in Iscove's ... Ishikawa(+) cells, unlike Ishikawa(-) cells, demonstrate estrogen-mediated gene expression. The difference in estrogen response ...
  https://www.thefreelibrary.com/DDT+and+its+metabolites+alter+gene+expression+in+human+uterine+cell+...-a098539828
*  Gene Therapy-Treated Stem Cells in Treating Patients Undergoing Stem Cell Transplant for Intermediate-Grade or High-Grade AIDS...
50,000 copies/ml by reverse transcriptase-polymerase chain reaction (RT-PCR) at the time of study enrollment ... Gene Therapy-Treated Stem Cells in Treating Patients Undergoing Stem Cell Transplant for Intermediate-Grade or High-Grade AIDS- ... Target number for untransduced cells in the final therapeutic cell product is 2.5 x 10^6 CD34+ cells/kg. Minimum target number ... The number and type of cells will be determined by fluorescence-activated cell sorting (FACS) analysis of the final cell ...
  https://clinicaltrials.gov/show/NCT00569985?order=112
*  Colon cancer stem cell markers CD44 and CD133 in patients with colorectal cancer and synchronous hepatic metastases
Typical results of gene expression by reverse transcriptase polymerase chain reaction are shown in Fig. 1. The mean ± SD ... colorectal adenocarcinomas with synchronous hepatic metastasis were analyzed by reverse transcriptase polymerase chain reaction ... studies focusing on mRNA expression using simple and practical methods such as reverse transcription polymerase chain reaction ... Reverse transcription was performed using the Moloney murine leukemia virus reverse transcriptase (M-MLV RT; Beams Biotech, ...
  https://www.spandidos-publications.com/10.3892/ijo.2015.2844
*  TMEM54 Gene - GeneCards | TMM54 Protein | TMM54 Antibody
Complete information for TMEM54 gene (Protein Coding), Transmembrane Protein 54, including: function, proteins, disorders, ... Specific detection of circulating tumor cells by reverse transcriptase-polymerase chain reaction of a beta-casein-like protein ... No data available for DME Specific Peptides for TMEM54 Gene Domains & Families for TMEM54 Gene Gene Families for TMEM54 Gene. ... Evolution for TMEM54 Gene. ENSEMBL:. Gene Tree for TMEM54 (if available). TreeFam:. Gene Tree for TMEM54 (if available). ...
  http://www.genecards.org/cgi-bin/carddisp.pl?gene=TMEM54&keywords=GH01G032952&prefilter=genomic_location
*  Disease-specific, neurosphere-derived cells as models for brain disorders | Disease Models & Mechanisms
... expression differences were validated for subsets of genes using quantitative reverse-transcriptase polymerase chain reaction ( ... CD105 and CD73 for mesenchymal stem cells; NES for neural stem cells; OCT4 for ES cells). The cells were immunopositive for β- ... We checked gene expression stability in one cell line from passages 7-25. At passages 10, 13, 16, 19, 22 and 25 the gene ... SZ-derived ONS cells displayed altered neurodevelopmental genes, pathways and function. We used microarray gene expression ...
  http://dmm.biologists.org/content/3/11-12/785.long
*  Mineralocorticoid Receptor Deficiency in T Cells Attenuates Pressure Overload-Induced Cardiac Hypertrophy and Dysfunction...
A, Quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) analysis of IL-2 (interleukin-2) gene expression in ... n=5:4:6:6:7:6. D, Quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) analysis of hypertrophy-related genes ... n=5:5:6:6. E, Quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) analysis of inflammatory genes in left ... quantitative reverse transcriptase polymerase chain reaction analysis revealed that cardiac expression of fetal genes, such as ...
  http://hyper.ahajournals.org/content/70/1/137
*  Effect of Titanium Surface Characteristics on the Behavior and Function of Oral Fibroblasts
Gene expression was analyzed via reverse transcriptase polymerase chain reaction. Collagen production and deposition were ... Attachment and proliferation of cells were examined by comparing the numbers of attached to detached cells and cell count, ... The cell counts on the machined and AE2 surfaces were higher, with a parallel orientation, whereas the cell count was lower and ... The AE2 surface contained fluoride atoms (2.45% 0.44% as F/Ti atomic ratio). Cell attachment was significantly weaker on the ...
  http://www.quintpub.com/journals/omi/abstract.php?iss2_id=364&article_id=4506&article=5&title=Effect%20of%20Titanium%20Surface%20Characteristics%20on%20the%20Behavior%20and%20Function%20of%20Oral%20Fibroblasts
*  Hemocyte production and maturation in an invertebrate animal; proliferation and gene expression in hematopoietic stem cells of...
Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology; Amino Acid, Transcription Factors/drug effects/genetics/ ... Cell Adhesion Molecules/drug effects/genetics/metabolism, Cell Differentiation/drug effects/physiology, Cell Lineage, ... Hemocyte production and maturation in an invertebrate animal; proliferation and gene expression in hematopoietic stem cells of ... Hematopoietic Stem Cells/drug effects/physiology, Hemocytes/cytology/drug effects/*physiology, Humans, In Situ Hybridization, ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:104966
*  British Library EThOS: Evaluation of corneal endothelial cell therapy using an in vitro human corneal model
... expression of PRDM genes in the corneal endothelium was undertaken using reverse transcriptase polymerase chain reaction (RT- ... with defined seeding cell density. The effect on stromal de-swelling in cell therapy treated corneas was assessed 3, 7 and 10 ... 12 cells (group 1), primary human corneal endothelial cells (hCECs) at 0 passage (group 2) and hCECs at passage 2 (group 3) ... Aim: To establish an in vitro human corneal decompensation model and to use it for the evaluation of a cell-therapy approach ...
  https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.731683
*  The DWF4 Gene of Arabidopsis Encodes a Cytochrome P450 That Mediates Multiple 22α-Hydroxylation Steps in Brassinosteroid...
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to isolate a cDNA clone. RNA was isolated from 5-day-old dark ... 1995). The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation. Genes Dev. 9, 97-107. ... using reverse transcriptase-polymerase chain reaction (RT-PCR). Whereas RNA from both light-grown and dark-grown seedlings ... 1995). The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in gibberellin biosynthesis. Plant Cell 7, 1307-1317 ...
  http://www.plantcell.org/content/10/2/231?ijkey=5ee3edc26e63188404812a5212518726926f7d5b&keytype2=tf_ipsecsha
*  Use of p53‐Silenced Endothelial Progenitor Cells to Treat Ischemia in Diabetic Peripheral Vascular Disease | Journal of the...
Quantitative reverse transcriptase polymerase chain reaction was used for gene expression analysis. Total mRNA from both HUVECs ... This outcome was confirmed by both gene expression (quantitative reverse transcriptase polymerase chain reaction) and OCR ... silenced cells. Interestingly, reverse transcriptase polymerase chain reaction of the quadriceps from this group for ... and platelet endothelial cell adhesion molecule 1. Similarly, quantitative reverse transcriptase polymerase chain reaction of ...
  http://jaha.ahajournals.org/content/6/4/e005146
*  IJERPH | Free Full-Text | Analysis of Gene Regulation in Rabbit Corneal Epithelial Cells Induced by Ultraviolet Radiation |...
RNA was extracted and amplified by reverse transcriptase-polymerase chain reaction using poly A+ specific anchoring primers and ... UV irradiation in rabbit cornea tissues using fluorescent differential display-reverse transcription-polymerase chain reaction ... Polyacrylamide gel electrophoresis revealed several differentially expressed genes in untreated versus UV irradiated cells. ... FDDRT-PCR). The epithelial cells were grown in standard medium for 2 or 4 hours post treatment. Cornea epithelial cells were ...
  http://www.mdpi.com/1660-4601/2/1/51/htm