Galectin-3 endocytosis by carbohydrate independent and dependent pathways in different macrophage like cell types.
... macrophage like cells, and the non-macrophage cell lines HFL-1... (More). BACKGROUND: Galectin-3 (the Mac-2 antigen) is ... macrophage like cells, and the non-macrophage cell lines HFL-1 fibroblasts and SKBR3 breast carcinoma. RESULTS: Galectin-3 ... and compared it between variants of a macrophage like cell line, and non-macrophage cells. METHODS: Endocytosis of galectin-3 ... and compared it between variants of a macrophage like cell line, and non-macrophage cells. METHODS: Endocytosis of galectin-3 ...https://lup.lub.lu.se/search/publication/2431447
Galectin-3 translocates to the immunological synapse and inhibits cytokine production in CD4+ T cells activated by engagement...
Injury- and antigen-specific control of CD4 T cell reactivity by CD4+CD25+ regulatory T cells (88.42) ... CD4+ T cells secreted more IFNγ and IL-4 compared to gal3+/+ cells. We also found that serum levels of IFNγ were significantly ... Galectin-3 is induced in CD4+ T cells upon activation by mitogens or TCR engagement, but its function in this cell type has not ... We conclude that galectin-3 is an inhibitory regulator of T cell activation that down-regulates signal transduction, and ...http://www.jimmunol.org/content/178/1_Supplement/S140.3
Loss of galectin-3 decreases the number of immune cells in the subventricular zone and restores proliferation in a viral model...
TMEV also reduced neuroblast and proliferative SVZ cell numbers in WT mice but this was restored in Gal-3(-/-) mice and was ... Because MS-induced inflammation may decrease SVZ proliferation and thus limit repair, we studied the role of galectin-3 (Gal-3 ... Though numerous CD45+ immune cells entered the SVZ of WT mice after TMEV infection, their numbers were significantly diminished ... reduced immune cell migration into the SVZ, reestablished SVZ proliferation and increased the number of progenitors in the ...https://www.ndcn.ox.ac.uk/publications/546924
Galectin-3 Shapes Antitumor Immune Responses by Suppressing CD8+ T Cells via LAG-3 and Inhibiting Expansion of Plasmacytoid...
... which are superior to conventional dendritic cells in activating CD8+ T cells. Thus, inhibiting galectin-3 in conjunction with ... T cells only in the tumor microenvironment. Galectin-3-deficient mice exhibit improved CD8+ T-cell effector function and ... T cells in vitro. Lastly, galectin-3-deficient mice have elevated levels of circulating plasmacytoid dendritic cells, ... Galectin-3 Shapes Antitumor Immune Responses by Suppressing CD8+ T Cells via LAG-3 and Inhibiting Expansion of Plasmacytoid ...http://cancerimmunolres.aacrjournals.org/content/early/2015/03/03/2326-6066.CIR-14-0150
Galectin-3 - Wikipedia
... cell activation and chemoattraction, cell growth and differentiation, cell cycle, and apoptosis. Given galectin-3's broad ... Companies have developed galectin modulators that block the binding of galectins to carbohydrate structures. The galectin-3 ... "Glycoprotein 90K/MAC-2BP interacts with galectin-1 and mediates galectin-1-induced cell aggregation". International Journal of ... is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix ...https://en.wikipedia.org/wiki/Galectin-3
Interaction of human neutrophils with endothelial cells regulates the expression of endogenous proteins annexin 1, galectin-1...
... galectin-1 and galectin-3. Autor Gil, C. D. La, M. Perretti, M. Oliani, S. M. ... Interaction of human neutrophils with endothelial cells regulates the expression of endogenous proteins annexin 1, galectin-1 ... Annexin 1 (ANXA1), galectin-1 (Gal-1) and galectin-3 (Gal-3) proteins have been identified as important mediators that promote ... Cell Biology International. London: Academic Press Ltd Elsevier B.V., v. 30, n. 4, p. 338-344, 2006.. ...http://repositorio.unifesp.br/handle/11600/28811
Galectin 3 293T transfected lysate (ab94225) | Abcam
Buy our Galectin 3 293T transfected lysate (positive control). ab94225 has been validated in western blot. Abcam now offers a ... ab94225 is a 293T cell transfected lysate in which Human Galectin 3 has been transiently over-expressed using a pCMV-Galectin 3 ... Cell Biology. Epigenetics. Metabolism. Developmental Biology. By research area. Immunology. Microbiology. Neuroscience. Signal ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ...http://www.abcam.com/galectin-3-overexpression-293t-lysate-whole-cell-ab94225.html
Poly N-acetyllactosamine substitutions on N- and not O-oligosaccharides or Thomsen-Friedenreich antigen facilitate lung...
Galectin-3 on vascular endothelium has been shown to facilitate lung specific metastasis. Metastatic variants of B16 melanoma ... Expression of galectins on microvessel endothelial cells and their involvement in tumour cell adhesion. Glycoconj. J. 11, 462- ... Versatile roles in cell-cell interaction. J. Cell Biol. 147, 467-470 (1999). doi: 10.1083/jcb.147.3.467 PubMedCrossRefGoogle ... Cell 127, 679-695 (2006). doi: 10.1016/j.cell.2006.11.001 PubMedCrossRefGoogle Scholar ...https://rd.springer.com/article/10.1007/s10719-008-9194-9
Galectin-3 Deficiency Accelerates High-Fat Diet-Induced Obesity and Amplifies Inflammation in Adipose Tissue and Pancreatic...
Type 1 T/NKT cells are increased in VAT in obese LGALS3−/− mice.. Significantly increased CD3+ T cells and CD3+NK1.1+ NKT cells ... Immune-mediated beta-cell destruction in vitro and in vivo-A pivotal role for galectin-3. Biochem Biophys Res Commun 2006;344: ... 2A) with markedly increased CD3+NK1.1+ IFN-γ+ type 1 NKT cells, and CD3+IFN-γ+ type 1 T cells were significantly higher ... Increased type 1 T and NKT cells and reduced Tregs in VAT of HFD-fed LGALS3−/− mice. A: Increased percentages of CD3+ T cells ...http://diabetes.diabetesjournals.org/content/62/6/1932.long
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices | Protocol
We rarely see cells stopping for more than 6 min, and find that, over a 3-hr period, they generally spend an average of 30 min ... Comte, I., et al. Galectin-3 maintains cell motility from the subventricular zone to the olfactory bulb. J. Cell Sci. 124, 2438 ... In a cell culture hood, cut away the most caudal third of the brain slice and remove any glue traces using fine straight ... Moreover, these cells are able to target sites of the brain affected by injury and neurodegeneration4,28. Being able to monitor ...https://www.jove.com/video/50905/in-vivo-postnatal-electroporation-time-lapse-imaging-neuroblast
CA-125 - Wikipedia
"The cancer antigen CA125 represents a novel counter receptor for galectin-1". Journal of Cell Science. 116 (Pt 7): 1305-18. doi ... MUC16 is also thought to participate in cell-to-cell interactions that enable the metastasis of tumor cells. This is supported ... Evidence suggests that expression of the cytoplasmic tail of MUC16 enables tumor cells to grow, promotes cell motility and may ... "Association of cell surface mucins with galectin-3 contributes to the ocular surface epithelial barrier". The Journal of ...https://en.wikipedia.org/wiki/CA-125
Detection of galectin-3 in tear fluid at disease states and immunohistochemical and lectin histochemical analysis in human...
... thus contributing to cell-cell and cell-matrix interaction,6-8 regulation of cell growth,9 10 and programmed cell death.11 In ... mast cells, and epithelial cells including corneal epithelium.2 6 14 15 It is a mitogen capable of stimulating fibroblast cell ... The inflammatory cells infiltrating the cornea express the galectin-3 in cytoplasm in contrast with absence of galectin-3 in ... Inflammatory cells (granulocytes and macrophages) that express galectin-3 were found in tear fluid samples which were positive ...http://bjo.bmj.com/content/85/11/1336
Circulating galectin-3 promotes m... preview & related info | Mendeley
Adhesion of circulating tumor cells to the blood vessel endothelium is a critical step in cancer metastasis. We show in this ... study that galectin-3, the concentration of which is greatly increased i... ... of the cell-surface MUC1 by causing MUC1 polarization, leading to exposure of smaller cell-surface adhesion molecules/ligands ... increases cancer cell adhesion to macrovascular and microvascular endothelial cells under static and flow conditions, increases ...https://www.mendeley.com/research-papers/circulating-galectin3-promotes-metastasis-modifying-muc1-localization-cancer-cell-surface/
Protocols and Video Articles Authored by Yves A. DeClerck
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor ... Consistently, bone marrow stromal cells express galectin-3, the receptor for galectin-3-binding protein. Supporting a role for ... As a result of the activity of these proteases, cell-cell and cell-ECM interactions are altered, new biologically active ECM ... An analysis of murine bones injected with hNBL cells revealed an increase of mVEGFA producing cells near tumor cells ...https://www.jove.com/author/Yves++A._DeClerck
PubMed Journals Articles About 'Neuropsychiatric Disorder Cluster Drug Development Pipeline Review 2017'
... where it is considered a marker of chondrogenic and osteogenic cell lineages. Galectin-3 protein was found to be increased in ... To determine drug penetration through skin, 5% of each promoter was used in an ex vivo system with human skin on Franz cells ... Role of Galectin-3 in Bone Cell Differentiation, Bone Pathophysiology and Vascular Osteogenesis. ... Galectin-3 is expressed in various tissues, including the bone, ...https://www.bioportfolio.com/resources/pubmed/?keyword=Neuropsychiatric+Disorder+Cluster+Drug+Development+Pipeline+Review+2017&
Lime Flavor PectaSol-C Stick Packs
Ongoing scientific research demonstrates PectaSol-C®'s natural ability to support healthy cell growth and behavior by binding ... Supports Healthy Cells & Blocks Galectin-3*. Ongoing scientific research demonstrates PectaSol-C®'s natural ability to support ... healthy cell growth and behavior by binding Galectin-3. MCP is a natural ligand for Galectin-3, a molecule present in large ... Due to its unique molecular structure, PectaSol-C® easily enters the blood stream to bind and block excess Galectin-3 ...https://www.econugenics.com/lime-infusion-pectasol-cr-stick-packs/
Frontiers | Extracellular Galectin-3 in Tumor Progression and Metastasis | Oncology
... such as cell adhesion, cell activation and chemoattraction, cell cycle, apoptosis and cell growth and differentiation. However ... such as cell adhesion, cell activation and chemoattraction, cell cycle, apoptosis and cell growth and differentiation. However ... the only chimera galectin found in vertebrates, is one of the best-studied galectins. It is expressed in several cell types and ... the only chimera galectin found in vertebrates, is one of the best-studied galectins. It is expressed in several cell types and ...https://www.frontiersin.org/articles/10.3389/fonc.2014.00138/full
Chaplin and Atopic Dermatitis
Sperms induced IgE production by seminal B cells from patients with atopic eczema via interaction of B cells with galectin-3 on ... Seminal B cells were cultured with sperms and IgE production was measured, while expression of galectin-3 on sperms was ... Post Chaplin, patients had a significant decrease in IgE production by seminal B cells as an allergic response to sperm cells. ... Viewing a humorous film decreases IgE production by seminal B cells from patients with atopic eczema.. Kimata H. ...http://denvernaturopathic.com/ChaplinandAtopicDermatitis.htm
Jurkat, Clone E6-1 ATCC ® TIB-152™ Homo sapiens peripheral b
This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line. ... The line was cloned from cells obtained from Dr. Kendall Smith and are mycoplasma free. ... The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally ... Expression of galectin-3 modulates T-cell growth and apoptosis. Proc. Natl. Acad. Sci. USA 93: 6737-6742, 1996. PubMed: 8692888 ...https://atcc.org/products/all/TIB-152.aspx
Proteomic analysis of human osteoprogenitor response to disordered nanotopography | Journal of The Royal Society Interface
On the flat surface (e-h) cells were fully spread and proliferated to form a confluent tissue layer (e,f). Also, (g,h) cells ... Upregulation of beta-galactoside-binding lectin precursor (galectin precursor) and galectin-1 was found. These results may ... Confluent cell sheets were trypsinized and 1×105 cells were seeded onto NSQ50 and control flat PCL sheets. The tissue culture ... 2003 Tensegrity I. Cell structure and hierarchical systems biology. J. Cell Sci. 116, 1157-1173. doi:10.1242/jcs.00359. ...http://rsif.royalsocietypublishing.org/content/6/40/1075
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Proteomics and Western blot of MDCK cells indicated aloe-emodin up-regulating galectin-3, and thioredoxin as well as down- ... Proteomic analysis of treated cells indicated galectin-3 up-regulation as one anti-influenza A virus action by aloe-emodin. ... The present study demonstrated that iAs significantly decreased cell viability and induced apoptosis in Neuro-2a cells. iAs ... OAS in infected cells, agreeing with expression pattern of those treated with aloe-emodin. Galectin-3 also inhibited influenza ...https://www.jove.com/visualize?author=Ching-Hong+Yang
PectaSol-C Modified Citrus Pectin
Ongoing scientific research demonstrates PectaSol-C®'s natural ability to support healthy cell growth and behavior by binding ... Supports Healthy Cells & Blocks Galectin-3*. Over the last decade, scientists and researchers have shown that cellular, ... Modified Citrus Pectin is the most-researched galectin-3 blocker, and PectaSol-C® is the most-researched modified citrus pectin ... galectin-3. Therefore, finding a way to maintain healthy galectin-3 levels is of utmost importance in supporting wellness and ...https://www.econugenics.com/pectasol-c-modified-citrus-pectin-main
PectaSol-C Modified Citrus Pectin
Ongoing scientific research demonstrates PectaSol-C®'s natural ability to support healthy cell growth and behavior by binding ... Supports Healthy Cells & Blocks Galectin-3*. Over the last decade, scientists and researchers have shown that cellular, ... Galectin-3 and Modified Citrus Pectin: Dr. Isaac Eliaz talks about galectin-3 and the impacts it has on human health. He also ... Modified Citrus Pectin is the most-researched galectin-3 blocker, and PectaSol-C® is the most-researched modified citrus pectin ...https://www.econugenics.com/shop-by-health-topic/men-s-health/pectasol-c-modified-citrus-pectin-main/
Frontiers | Drugs and Targets in Fibrosis | Pharmacology
In liver and pancreas, precursor cells like hepatic stellate cells (HSC) (Moreira, 2007) and pancreatic stellate cells (Apte et ... Li, L. C., Li, J., and Gao, J. (2014). Functions of galectin-3 and its role in fibrotic diseases. J. Pharmacol. Exp. Ther. 351 ... These recruited immune cells function as the scavenger to remove tissue debris and dead cells, resulting in acute inflammation ... Finally, immune cells undergo apoptosis and epithelial/endothelial cells proliferate to regenerate injury sites, leading to ...https://www.frontiersin.org/articles/10.3389/fphar.2017.00855/full?utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Pharmacology&id=306391
Patent US7416725 - Compositions and methods of using galectin-8 as an inhibitor of tumor cell ... - Google Patents
It further provides compositions and methods for modulating the expression of galectin-8, and galectin-8-like proteins, ... and methods for the use thereof as physiological modulators of cell adhesion and in treatment of tumors, both in vivo or ex ... The present invention provides compositions that enhance or inhibit the interactions of galectin-8 and galectin-8-like proteins ... with other extracellular matrix proteins or cell surface receptors, ...http://www.google.com/patents/US7416725?dq=7181427