... overlaps Pax-2 (brown) in the ventral IZ. All En-1 cells express Pax-2. Boundaries of the ventral Pax-2 domain are indicated by ... To begin to examine the role of transcription factors in spinal cord interneurons, we have analyzed the cells expressingEn-1 in ... This gene may mark the ventral limit of the dorsal territory of cell differentiation in the spinal cord and indicates that the ... 1993) Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc Natl Acad Sci USA 90: ...http://www.jneurosci.org/content/17/20/7805
Knockdown of def6 does not alter mesodermal cell fate s | Open-i
Knockdown of def6 does not alter mesodermal cell fate specification and anterior-posterior patterning.Uninjected and def6 MO- ... are involved in ventral cell fate specification. The non-axial marker caudal homeobox transcription factor 4 (cdx4; I and J; 19 ... all known to be involved in cell fate specification. The expression pattern of the dorsalising factors chordin (chd; Figure 4A ... At 10 hpf, expression of the anterior specific genes krox20 (M and N; 37/41 embryos) and pax2 (O and P; 16/19 embryos,) ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3198796_pone.0026548.g004&req=4
Protocols and Video Articles Authored by Stefan Britsch (Translated to German)
Homeodomain and bHLH transcription factors can control the development of neuronal cell types in the dorsal horn. Here, we ... In a screen for novel factors we identified the gene encoding the neuropeptide Galanin in mouse. At embryonic day (E) 8.5 ... gene Olig3 is expressed in progenitor cells that generate class A (dI1-dI3) neurons and that Olig3 is an important factor in ... The homeobox gene Sax2 is expressed predominantly in the brainstem, in the vicinity of serotonergic neurons, and in the ventral ...https://www.jove.com/author/Stefan_Britsch?language=German
Zebrafish Pronephros Development | SpringerLink
Marra AN, Wingert RA (2016) Epithelial cell fate in the nephron tubule is mediated by the ETS transcription factors etv5a and ... Bouchard M, Pfeffer P, Busslinger M (2000) Functional equivalence of the transcription factors Pax2 and Pax5 in mouse ... Reggiani L, Raciti D, Airik R, Kispert A, Brandli AW (2007) The prepattern transcription factor Irx3 directs nephron segment ... James RG, Schultheiss TM (2005) Bmp signaling promotes intermediate mesoderm gene expression in a dose-dependent, cell- ...https://link.springer.com/chapter/10.1007/978-3-319-51436-9_2
PPT - PY4302 Developmental Neuroscience Eye Development PowerPoint presentation | free to download - id: 2e02a-NTYyO
How does an apparently uniform sheet of neural precursor cells differentiate ... DACH (dachshund) Shown to interact with eya. ... transcription factors imposes dorso-ventral and naso-temporal specificity in cells within the developing optic cup.. These ... Gene X Gene Y. Gene X Gene Y. Gene X Gene Y. time 51. Pax6 is required for eye development in mice and flies. Pax6/. Pax6/. ... Transcription factor control of retinal cell specification. Two classes of transcription factor basic helix-loop-helix (bHLH) ...https://www.powershow.com/view/2e02a-NTYyO/PY4302_Developmental_Neuroscience_Eye_Development_powerpoint_ppt_presentation?varnishcache=1
Frontiers | Transcription factors define the neuroanatomical organization of the medullary reticular formation | Frontiers in...
Within each lineage, distinct patterns of gene expression and organization were localized to specific hindbrain regions. Rostro ... populations were both present in partially overlapping stripes within the reticular formation extending from dorsal to ventral ... are derived from distinct developmental progenitor domains each of which expresses distinct patterns of transcription factors ( ... neurons are derived from distinct developmental progenitor domains each of which expresses distinct patterns of transcription ...https://www.frontiersin.org/articles/10.3389/fnana.2013.00007/full
Bcl11a is required for neuronal morphogenesis and sensory circuit formation in dorsal spinal cord development | Development
LIM homeobox or TLX families of transcription factors are involved in cell fate decisions, morphogenesis, positioning and ... 2001). The transcription factor Sox10 is a key regulator of peripheral glial development. Genes Dev. 15, 66-78. ... 1995). A cre-transgenic mouse strain for the ubiquitous deletion of loxP-flanked gene segments including deletion in germ cells ... TFCP2L1 represses multiple lineage commitment of mouse embryonic stem cells through MTA1 and LEF1. Kuisheng Liu, Yan Zhang, ...http://dev.biologists.org/content/139/10/1831
Frontiers | The Long Adventurous Journey of Rhombic Lip Cells in Jawed Vertebrates: A Comparative Developmental Analysis |...
The fate of the ptf1a expressing ventral LRL zone which gives rise to (excitatory cells of) the inferior olive (climbing fiber ... development covering classic approaches up to modern developmental genetics which identifies the relevant differential gene ... The fate of the ptf1a expressing ventral LRL zone which gives rise to (excitatory cells of) the inferior olive (climbing fiber ... excitatory granule cells) and an early ventral migration into the anterior rhombencephalon (cholinergic nuclei). As for the ...https://www.frontiersin.org/articles/10.3389/fnana.2011.00027/full
Specification of epibranchial placodes in zebrafish | Development
PPE is defined by expression of specific transcription factors, including the homeobox factor Six1 and its binding partner Eya1 ... Note ectopic phox2b-positive cells on the ventral surface of the head as well as in the eye (arrowheads). Formation of the ... Gene Expr. Patterns 2, 183-188.. OpenUrlCrossRefPubMed. *↵ Torres, M., Gomez-Pardo, E. and Gruss, P. (1996). Pax2 contributes ... Although our misexpression experiments demonstrated that most of the PPE and ventral embryonic ectoderm are competent to ...http://dev.biologists.org/content/134/3/611
Frontiers | Making sense of Wnt signaling-linking hair cell regeneration to development | Frontiers in Cellular Neuroscience
At later stages, its activity governs sensory hair cell specification, cell cycle regulation, and hair cell orientation. In ... At later stages, its activity governs sensory hair cell specification, cell cycle regulation, and hair cell orientation. In ... This review describes the current knowledge of the roles of Wnt signaling and Wnt-responsive cells in hair cell development and ... This review describes the current knowledge of the roles of Wnt signaling and Wnt-responsive cells in hair cell development and ...https://www.frontiersin.org/articles/10.3389/fncel.2015.00066/full
From placode to polarization: new tunes in inner ear development | Development
It is not clear whether these factors also directly regulate ventral gene expression, or if the defects in gene expression are ... The transcription factor Pax2 is among the earliest of the `regionalized' genes in the chick inner ear. It is first expressed ... of stem cells into the otocysts of embryonic chicks has resulted in the formation of stem cell-derived hair cells (Li et al., ... ventral phenotypes are induced through a combination of the expression of the transcription factor Six1 (orange) in the ventral ...http://dev.biologists.org/content/131/17/4119
Interactive Fly, Drosophila
It has therefore been predicted that the phenotype caused by knockdown of a character identity gene (i.e. transcription factor ... The four vertebrate genes have a role in establishing cell fates within the limits of various embryonic brain regions. They act ... Consistent with this interpretation, expression of the Otx-2 gene (Drosophila homolog: Orthodenticle), is lost in the ventral ... bHLH transcription factors play important roles in a variety of developmental processes, including corticogenesis. ME2 belongs ...http://www.sdbonline.org/sites/fly/segment/emptysp2.htm
Three-dimensional induction of dorsal, intermediate and ventral spinal cord tissues from human pluripotent stem cells |...
2012). Renshaw cell interneuron specialization is controlled by a temporally restricted transcription factor program. ... cells were distributed separately (Fig. 3L,N,O). Only a few Brn3a+/Islet1+ cells and PAX2+/Lhx1+ cells were detected (Fig. 3M,P ... 2014). 3D reconstitution of the patterned neural tube from embryonic stem cells. Stem Cell Reports 3, 987-999. doi:10.1016/j. ... Because each progenitor domain can be distinguished by the expression pattern of distinct transcription factors, we first ...http://dev.biologists.org/content/145/16/dev162214.full
... we demonstrate that the transcription factor Ptf1a is capable of cell autonomously redirecting cortical pyramidal cell identity ... 2001) Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol 240:237-246, doi:10.1006/ ... observed lack of GABA was further supported by an absence of Pax2 and Lhx1 expression-two Ptf1a-dependent transcription factors ... The retention of Pax6 expression in some Ptf1a-misexpressing cells reflected their dorsal rather than ventral telencephalic ...http://www.jneurosci.org/content/35/15/6028
Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity |...
... which activate superimposing waves of intrinsic factors restricting their identity and fate. The transcription factor Otx2 ... Purkinje cells (O-T, red arrows), Pax2+ Lhx1- Parv- presumptive Golgi cells (O-R, yellow arrowheads), Lhx1+ Parv+ lowPax2+ ... Data from the present study suggest that, in an embryonic context where MHB integrity is retained, Otx2 may cell-autonomously ... We found that the Pax7+ m1 domain can be further subdivided into a dorsal Zic1+ m1a and a ventral Zic1- m1b sub-domain. Loss of ...http://dev.biologists.org/content/141/2/377
Zebrafish foxi one modulates cellular responses to Fgf signaling required for the integrity of ear and jaw patterning |...
... of ventral cartilages and at least partly functions through the induction of the transcription factor hand2 in ventral NC cells ... pax2, pax5 and pax8 are believed to have arisen from a single ancestral pax2/5/8 gene (Pfeffer et al., 1998). Two recent ... Identification of nine tissue-specific transcription factors of the hepatocyte nuclear factor 3/forkhead DNA-binding-domain ... with each segment deriving from an embryonic pharyngeal arch, as well as a dorsal-to-ventral (DV) polarity within individual AP ...http://dev.biologists.org/content/130/11/2543
Distinct domains within Mash1 and Math1 are required for function in neuronal differentiation versus neuronal cell-type...
The neural bHLH factors belong to a much larger family of transcription factors that are essential for diverse processes such ... A mammalian helix-loop-helix factor structurally related to the product of the Drosophila proneural gene atonal is a positive ... 2A,I, red), Lhx1/5+;Pax2- cells (dI2) (Fig. 2B,J, red) and Lhx1/5+;Pax2+ cells (dI4/6) (Fig. 2B,L, yellow). The increase in ... colleagues have demonstrated that higher order complex formation of homeodomain factors and bHLH factors control ventral cell ...http://dev.biologists.org/content/131/6/1319
Identification of Pax2-regulated genes by expression profiling of the mid-hindbrain organizer region | Development
The promoter of the CD19 gene is a target for the B-cell-specific transcription factor BSAP. Mol. Cell. Biol. 12,2662 -2672. ... The transcription factors En2 and Brn1, as well as the signaling modifiers Sef and Tapp1, implicate Pax2 in the establishment ... The seven-transmembrane receptor Smoothened cell-autonomously induces multiple ventral cell types. Nat. Neurosci. 3,41 -46. ... giving rise to heterogeneous transcription initiation in embryonic brain cells. ...http://dev.biologists.org/content/132/11/2633
The level of sonic hedgehog signaling regulates the complexity of cerebellar foliation | Development
Matise, M., Auerbach, W. and Joyner, A. L. (2000). Production of targeted embryonic stem cell clones. In Gene Targeting: A ... All Shh signaling in patterning the mouse spinal cord is mediated by the three Gli transcription factors (Bai, 2004; Wijgerde ... Furthermore, cell death is not a contributing factor to the cerebellar phenotypes in En1-Gli2 cko or Nes-Smo cko mutants, as ... In the spinal cord, Shh signaling through Gli2 is required for the induction of the two most ventral cell types (Matise et al ...http://dev.biologists.org/content/133/9/1811
Neurogenin 2 is required for the development of ventral midbrain dopaminergic neurons | Development
... together with other transcription factors, may contribute to enhancing the DA differentiation of stem/precursor cells. Such ... The HMG-box transcription factor Sox2 is expressed by most progenitors of the developing CNS, and is generally downregulated by ... preLights - Rapid embryonic cell cycles defer the establishment of heterochromatin by Eggless/SetDB1 in Drosophila. ... Ma, Q., Kintner, C. and Anderson, D. J. (1996). Identification of neurogenin, a vertebrate neuronal determination gene. Cell 87 ...http://dev.biologists.org/content/133/3/495?ijkey=eadd8e6879b81b82c6c3fd16751307a7e6be6435&keytype2=tf_ipsecsha
Neurogenin 2 is required for the development of ventral midbrain dopaminergic neurons | Development
... together with other transcription factors, may contribute to enhancing the DA differentiation of stem/precursor cells. Such ... The HMG-box transcription factor Sox2 is expressed by most progenitors of the developing CNS, and is generally downregulated by ... Ma, Q., Kintner, C. and Anderson, D. J. (1996). Identification of neurogenin, a vertebrate neuronal determination gene. Cell 87 ... and Pax2 and Pax5 (Urbanek et al., 1997), that contribute to the development of most neuronal cell types in the mid- and ...http://dev.biologists.org/content/133/3/495
Msx1 and Msx2 act as essential activators of Atoh1 expression in the murine spinal cord | Development
2010). How can conserved gene expression allow for variation? Lessons from the dorso-ventral patterning gene muscle segment ... 2007). Bmp and Wnt/β-catenin signals control expression of the transcription factor Olig3 and the specification of spinal cord ... TFCP2L1 represses multiple lineage commitment of mouse embryonic stem cells through MTA1 and LEF1. Kuisheng Liu, Yan Zhang, ... Within this cascade, Msx1 and Msx2, two homeodomain transcription factors (TFs), are induced earlier than bHLH neuralizing TFs ...http://dev.biologists.org/content/141/8/1726
Helt determines GABAergic over glutamatergic neuronal fate by repressing Ngn genes in the developing mesencephalon | Development
bHLH network in cell fate specification in the mesencephalon. In many developmental systems, bHLH-O type transcription factors ... structure and developmental expression of Megane bHLH factor. Gene 377,65 -76. ... Note that some Helt+ cells are observed in the ventral-most region of the m1 domain at E11.5. These Helt+ cells in the m1 ... TFCP2L1 represses multiple lineage commitment of mouse embryonic stem cells through MTA1 and LEF1. Kuisheng Liu, Yan Zhang, ...http://dev.biologists.org/content/134/15/2783
Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling | Development
Ventral Lbx1 cells were scored as the number of Lbx1 cells below the midpoint of the dorsoventral axis. Mean ± s.e.m. plotted ... 2006). The transcription factor NFIA controls the onset of gliogenesis in the developing spinal cord. Neuron 52, 953-968. ... 2003). Bhlhb5 is expressed in the CNS and sensory organs during mouse embryonic development. Gene Expr. Patterns 3, 755-759. ... F) Summary of the domain relationship between Bhlhb5, HD transcription factors, Notch ligands and Notch pathway activity under ...http://dev.biologists.org/content/138/15/3199.long
Enhancer evolution and animal diversity | Philosophical Transactions of the Royal Society B: Biological Sciences
2012 A transcription factor collective defines cardiac cell fate and reflects lineage history. Cell 148, 473-486. (doi:10.1016/ ... 2012 DNA regions bound at low occupancy by transcription factors do not drive patterned reporter gene expression in Drosophila ... 2011 Combinatorial binding in human and mouse embryonic stem cells identifies conserved enhancers active in early embryonic ... pax2 is expressed in several cell-types, whereas pax8 is expressed only in a subset of pax2-expressing tissues. Despite this ...http://rstb.royalsocietypublishing.org/content/368/1632/20130017