Handling Metalloproteinases - Current Protocols
... is polarized by a catalytic metal, usually a zinc ion, and a general base, usually the carboxyl group of a glutamic acid side ... 1 reveal a conserved catalytic domain and a disintegrin‐like domain with a fold homologous to cysteine‐rich domains. J. Mol. ... Large‐scale expression, refolding, and purification of the catalytic domain of human macrophage metalloelastase (MMP‐12) in ... the catalytic activity. As a consequence, these enzymes are generally sensitive to metal chelators. Moreover, the catalytic ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ps2116.html
SSDB Motif Search Result: mtu:Rv0126
Alpha amylase, catalytic domain 5.1e-76. -. pf:hDGE_amylase. 82. 152. Glycogen debranching enzyme, glucanotransferase domain ... Domain of unknown function (DUF3459) 2.7e-05. -. pf:Malt_amylase_C. 501. 581. Maltogenic Amylase, C-terminal domain 2.7e-22. - ...http://www.kegg.jp/ssdb-bin/ssdb_motif?kid=mtu:Rv0126
Chimeric CESA and CESA/CSLD proteins exchanging the N-terminal region (Wang et al., 2006) and catalytic domain (Park et al., ... 2006) Chimeric proteins suggest that the catalytic and/or C-terminal domains give CesA1 and CesA3 access to their specific ... As a positive control, the ALG5 protein was fused to the wild-type ubiquitin domain. In contrast to the I13G mutant (NubG), the ... 2000) Multiple cellulose synthase catalytic subunits are required for cellulose synthesis in Arabidopsis. Plant Cell 12: 2529- ...http://www.plantphysiol.org/content/160/2/726
The ADP-ribose polymerase Tankyrase regulates adult intestinal stem cell proliferation during homeostasis in Drosophila |...
... poly-ADP-ribose polymerase catalytic domain. Percentage similarity between dTnks and hTNKS is indicated above each domain. (B) ... 2011). Yan, an ETS-domain transcription factor, negatively modulates the Wingless pathway in the Drosophila eye. EMBO Rep. 12, ... Tnks mutants display increased mortality under reduced nutrient conditions. (A) Schematic representation of domains in ... domain (Fig. 1A) (Sbodio et al., 2002; Smith et al., 1998). ARC2, ARC4 and ARC5 in the mammalian TNKS proteins, which bind axin ...http://dev.biologists.org/content/143/10/1710
Method of screening for compounds which affect the processing of EphA4 by .gamma.-secretase - Patent # 7910324 -...
domain in its C-terminal region, expressed in a large variety of cells in manymammals. In human, APP is encoded by the gene APP ... Presenilin is the catalyticdomain; the presenilin gene has been identified as a causative gene for familial Alzheimer's disease ... Since this band is equal in size to the region spreading from the transmembrane domain of EphA4 to its C-terminal site, it has ... Since this band is equal in size to the region spreading from the transmembrane domain of EphA4 toits C-terminal site, it has ...http://www.patentgenius.com/patent/7910324.html
SPO14 Separation-of-Function Mutations Define Unique Roles for Phospholipase D in Secretion and Cellular Differentiation in...
Separation-of-function mutations map to the catalytic domain of PLD. Boxed regions denote domains in Spo14 based on ... Separation-of-function mutations map to the catalytic domain of PLD: Sequence analyses of the mutant alleles showed that, in ... catalytic regions I-IV (Morriset al. 1996; Sunget al. 1997); PIP2, PIP2 binding domain (Sciorraet al. 1999). The first putative ... 1997 Novel domains in NADPH oxidase subunits, sorting nexins, and PtdIns 3-kinases: binding partners of SH3 domains? Protein ...http://www.genetics.org/content/158/4/1431
Loss of Collectrin, an Angiotensin-Converting Enzyme 2 Homolog, Uncouples Endothelial Nitric Oxide Synthase and Causes...
Unlike angiotensin-converting enzyme 2, collectrin lacks any catalytic domain. Collectrin has been shown to function as a ... collectrin lacks any catalytic domain.2 Collectrin is highly conserved among species, sharing ,80% identity between mice, rats ...http://circ.ahajournals.org/content/128/16/1770
Prkcb protein kinase C, beta [Rattus norvegicus (Norway rat)] - Gene - NCBI
S_TKc; Serine/Threonine protein kinases, catalytic domain. cd05616. Location:341 → 664. STKc_cPKC_beta; Catalytic domain of the ... S_TKc; Serine/Threonine protein kinases, catalytic domain. cd05616. Location:341 → 663. STKc_cPKC_beta; Catalytic domain of the ... S_TKc; Serine/Threonine protein kinases, catalytic domain. cd05616. Location:341 → 664. STKc_cPKC_beta; Catalytic domain of the ... Conserved Domains (4) summary. cd04026. Location:159 → 289. C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and ...https://www.ncbi.nlm.nih.gov/gene?LinkName=pubmed_gene_rif&from_uid=21215369
Protocols and Video Articles Authored by Thomas Schneider
Nine of these mutations involved the catalytic domain. Although affected siblings had similar clinical features, the relation ... Four source domains are distinguished; that is, production, handling of bulk product, dispersion of ready-to-use nanoproducts, ... Jul, 2008 , Pubmed ID: 18460546 Rapid alignment of proteins in terms of domains (RAPIDO) is a web server for the 3D alignment ... Frequency Domain Aperture for the Gain Bandwidth Reduction of Stimulated Brillouin Scattering Optics Letters. Mar, 2012 , ...https://www.jove.com/author/Thomas_Schneider
Human MMP-13 Antibody AF511: R&D Systems
Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic ... domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. ...https://www.rndsystems.com/products/human-mmp-13-antibody_af511
Ubiquitin‐specific protease 4 is inhibited by its ubiquitin‐like domain | EMBO Reports
USP4 has a Ubl domain N‐terminal of its catalytic domain, but it has an additional Ubl domain embedded in the catalytic domain. ... domain inserted into the catalytic domain has autoregulatory function. This Ubl domain can bind to the catalytic domain and ... The catalytic domain of USP4-D1D2. (A) Domain architecture of USP4 and fragments expressed. (B) Crystal structure of USP4-D1D2 ... We compared the catalytic activity of the USP4 catalytic domain with and without the large insert, by using in vitro ...http://embor.embopress.org/content/12/4/365
Role of Cytokines in Systemic Lupus Erythematosus: Recent Progress from GWAS and Sequencing
Y305C is a missense coding mutation located outside of the catalytic domain. Among Africans, five cases had a mutation at E266G ... 14] identified a significant association between SLE risk and locus between IRF7 and the PHRF1 (PHD and ring finger domains 1) ... 124] examined a nonsynonymous SNP within the transmembrane domain, which was significantly associated with SLE in Chinese, ... The SNP (rs1050501) led to an aminoacid substitution within the transmembrane domain at position 232, isoleucine to tryptophan ...https://www.hindawi.com/journals/bmri/2012/798924/
ADAMTS-7 Inhibits Re-endothelialization of Injured Arteries and Promotes Vascular Remodeling Through Cleavage of Thrombospondin...
Catalytic domain of ADAMTS-7 (TS7-CD) digested hTSP-1. Purified hTSP-1 was incubated with the catalytic domain of ADAMTS-7 in ... As shown in Figure 6F, the catalytic domain of ADAMTS-7 digested TSP-1 in a dose-dependent manner. ... The recombinant catalytic domain of ADAMTS-7 was incubated with purified human TSP-1 in a buffer containing 50 mmol/L Tris-HCl ... we purified the catalytic domain (aa 217-427) of rADAMTS-7 as a glutathione-S-transferase fusion protein in bacteria. The ...http://circ.ahajournals.org/content/131/13/1191
Quantitative Western blots measuring steady-state level | Open-i
Catalytic and functional roles of conserved amino acids in the SET domain of the S. cerevisiae lysine methyltransferase Set1. ... Catalytic and functional roles of conserved amino acids in the SET domain of the S. cerevisiae lysine methyltransferase Set1. ... To improve understanding of the catalytic mechanism of Set1, single amino acid substitutions were made within the SET domain. ... To improve understanding of the catalytic mechanism of Set1, single amino acid substitutions were made within the SET domain. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3585878_pone.0057974.g003&req=4
a) TLC identification of 5hmC in A. mellifera. Single | Open-i
Here, we show that the honeybee TET (AmTET) catalytic domain has dioxygenase activity and converts 5mC to 5- ... Here, we show that the honeybee TET (AmTET) catalytic domain has dioxygenase activity and converts 5mC to 5- ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4150289_rsob-4-140110-g1&req=4
Formation of a cis WC/WC G9-A20 base pair in the A730 l | Open-i
Catalytic Domain. *Magnesium/chemistry. *Models, Molecular. *Molecular Sequence Data. *Nuclear Magnetic Resonance, Biomolecular ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3105416_gkq1244f4&req=4
Hagai Tavori - Publications - Oregon Health & Science University
Farbstein, D. , Blum, S. , Pollak, M. , Asaf, R. , Viener, H. L. , Lache, O. , Asleh, R. , Miller-Lotan, R. , Barkay, I. , Star, M. , Schwartz, A. , Kalet-Littman, S. , Ozeri, D. , Vaya, J. , Tavori, H. , Vardi, M. , Laor, A. , Bucher, S. E. , Anbinder, Y. , Moskovich, D. & 4 others Abbas, N., Perry, N., Levy, Y. & Levy, A. P. Nov 2011 In : Atherosclerosis. 219, 1, p. 240-244 5 p.. Research output: Contribution to journal › Article ...https://ohsu.pure.elsevier.com/en/persons/hagai-tavori/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle
Identification of a RAC/AKT-like gene in Leishmania parasites as a putative therapeutic target in leishmaniasis | Parasites &...
... domain, which binds phosphoinositides with high affinity; a central catalytic domain; and a C-terminal regulatory domain . ... to the PH domain of AKT, and subsequent phosphorylation at two key residues, Thr308 and Ser473, located at the catalytic domain ... Additional file 2: Figure S2. Alignment of the catalytic domains of the distinct RAC/AKT-like proteins present in Leishmania ... The last group does not contain the PH domain, but it is included here because of its homology regarding the other two domains ...https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-017-2379-y
Anti-Dnmt3L antibody (ab3493) | Abcam
Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the ...http://www.abcam.com/dnmt3l-antibody-ab3493.html
PCSK9 Gene - GeneCards | PCSK9 Protein | PCSK9 Antibody
Its proteolytic activity is autoinhibited by the non-covalent binding of the propeptide to the catalytic domain. Inhibited by ... Domain:. * The C-terminal domain (CRD) is essential for the LDLR-binding and degrading activities. ... The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing ... The pro-PCSK9 form (via C-terminal domain) interacts with LDLR. Interacts (via the C-terminal domain) with ANXA2 (via repeat ...http://www.genecards.org/cgi-bin/carddisp.pl?id_type=entrezgene&id=255738
SSDB Motif Search Result: gth:Geoth 2861
e3 binding domain 1.4e-14. -. pf:2-oxoacid_dh. 206. 434. 2-oxoacid dehydrogenases acyltransferase (catalytic domain) 1.6e-91. - ...http://www.kegg.jp/ssdb-bin/ssdb_motif?kid=gth:Geoth_2861
Complement Component C1r Antibody [Unconjugated] (AF1807): Novus Biologicals
The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1‑705) of human C1r was ... The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. ...https://www.novusbio.com/products/complement-component-c1r-antibody_af1807
A single-domain llama antibody potently inhibits the enzymatic activity of botulinum neurotoxin by binding to the non-catalytic...
A single-domain llama antibody potently inhibits the enzymatic activity of botulinum neurotoxin by binding to the non-catalytic ... we created a library of non-immune llama single-domain VHH (camelid heavy-chain variable region derived from heavy-chain-only ...https://www.omicsonline.org/references/a-singledomain-llama-antibody-potently-inhibits-the-enzymatic-activity-of-botulinum-neurotoxin-by-binding-to-the-noncatalytic-alphaexosite-binding-region-575715.html
Biology | Free Full-Text | A Comparison of Two Single-Stranded DNA Binding Models by Mutational Analysis of APOBEC3G
Several high-resolution structures of the APOBEC3G catalytic domain have been generated, but none reveal how this enzyme binds ... Several high-resolution structures of the APOBEC3G catalytic domain have been generated, but none reveal how this enzyme binds ... These observations were supported by A3G catalytic domain-ssDNA chemical shift perturbation experiments. The overall data set ... Concordant with both models, changing the conserved arginine at position 313 to glutamate abolished both catalytic and ...http://mdpi.com/2079-7737/1/2/260/xml
Protein alignment agains all known databases - Biology-Online
E.g. catalytic domain, localization domain, dimerization domain... In my case, the catalytic domain is known, and I want to ... What I need is actually to look for other domains. Is there a tool somewhere that can look for all known domains within a given ... So, the your identified domain is spanning whole sequence or not?. no. say, a protein is ~300 residues; one domain is known - ... if you have detected some domains, you can't have other domains in the same sequence! ...https://www.biology-online.org/biology-forum/viewtopic.php?t=18762&hilit=Blast
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