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*  Lirias: On the dephosphorylation of the ATP,Mg-dependent protein phosphatase modulator
The phosphorylase phosphatase activity of the catalytic subunit is inhibited by nanomolar concentrations of phosphoinhibitor-1 ... but can be abolished by phosphoinhibitor-1 in concentrations comparable to the amount of modulator used as substrate ( ... On the dephosphorylation of the ATP,Mg-dependent protein phosphatase modulator. Authors: Vandenheede, Jackie ×. Vanden Abeele, ... Mg-dependent protein phosphatase. Mg2+ is implicated in this autocatalytic dephosphorylation which is not effected by the ...
  https://lirias.kuleuven.be/handle/123456789/10205
*  Frontiers in Bioscience 4, d270-285, March 15, 1999]
... of synthase phosphatase/phosphorylase phosphatase activities of tissue extracts differed from that of the purified catalytic ... Enzyme activity was assayed using phosphorylase a as the substrate (79). Y272 is located close to the active site with its ... The glycogen bound form of phosphorylase phosphatase - the targeting hypothesis 4. Recombinant Protein Phosphatase-1 Catalytic ... Protein phosphatase-1, originally studied as phosphorylase phosphatase, is one of the major ser/thr protein phosphatases. It ...
  https://www.bioscience.org/1999/v4/d/lee/fulltext.htm
*  Phosphatase - Wikipedia
Phosphatases are classified by substrate specificity and sequence homology in catalytic domains. Despite their classification ... Docking interactions can also allosterically regulate phosphatases and thus influence their catalytic activity. In contrast to ... Phosphatase enzymes are not to be confused with phosphorylase enzymes, which catalyze the transfer of a phosphate group from ... Thus, one phosphatase can exhibit the qualities of multiple phosphatase families. Acid phosphatase Alkaline phosphatase ...
  https://en.wikipedia.org/wiki/Phosphatase
*  Protein phosphatase 1 - Wikipedia
This binding to phosphorylase a prevents any phosphatase activity of PP1 and maintains the glycogen phosphorylase in its active ... The catalytic subunit is highly conserved among all eukaryotes, thus suggesting a common catalytic mechanism. The catalytic ... These regulatory subunits play an important role in substrate specificity as well as compartmentalization. Some common ... Protein phosphatase 1 (PP1) belongs to a certain class of phosphatases known as protein serine/threonine phosphatases. This ...
  https://en.wikipedia.org/wiki/Protein_phosphatase_1
*  2nym - Proteopedia, life in 3D
PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B ... The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of ... Crystal Structure of Protein Phosphatase 2A (PP2A) with C-terminus truncated catalytic subunit. ... The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit ...
  http://proteopedia.org/wiki/index.php/2nym
*  Plus it
We measured phosphatase activity in the absence of Ca2+ with phosphorylase a as substrate (Fig.1, control) where only PP1 and ... The main myosin light chain protein phosphatases (PPs) are PP1 and PP2A, which are comprised of catalytic and one or more ... Aliquots of homogenates were used for determination of phosphatase activity.. Phosphatase Assay.. Phosphatase activity was ... Effects of Cant (○) and OA (●) on phosphatase activity (n= 3) in homogenates of rat aorta. Phosphatase activity is expressed as ...
  http://jpet.aspetjournals.org/content/294/2/620
*  Phosphoprotein Phosphatases Serine Threonine | China-Mainland | Sigma-Aldrich
Type 2 phosphatases were further divided depending on their divalent cation requirements for full activity: phosphatase 2A ( ... Sequences of the catalytic domains of PPP family phosphatases are highly related to one another. They have been highly ... Type 1 protein phosphatases (PP1) dephosphorylate the β-subunit of phosphorylase kinase and are inhibited by phospho-inhibitor- ... Prior to the knowledge of their sequence, phosphoserine/threonine phosphatases were classified on the basis of their substrate ...
  http://www.sigmaaldrich.com/china-mainland/zh/technical-documents/articles/biology/rbi-handbook/intracellular-signaling-enzymes-receptors/phosphoprotein-phosphatases-serine-threonine.html
*  JCI - LRH-1-dependent glucose sensing determines intermediary metabolism in liver
GP, glycogen phosphorylase; GS, glycogen synthase; G6Pase, glucose-6-phosphatase. (B-D) Glucose fluxes in Lrh1fl/fl mice (white ... This is achieved through regulatory complexes that modulate both the catalytic activity and the expression level of metabolic ... This metabolite serves as the substrate for the oxidation of glucose as well as its storage as glycogen and fat. Hepatic ... Luciferase activities were determined 24 hours later and normalized for β-galactosidase activities. Luciferase activities in ...
  https://www.jci.org/articles/view/62368
*  Phosphorylase kinase - Wikipedia
"Two structures of the catalytic domain of phosphorylase kinase: an active protein kinase complexed with substrate analogue and ... However, this proposed catalytic activity has yet to be proven directly. calmodulin glycogenolysis Berg, J., Tymoczko, J. & ... When the cell needs to stop glycogen breakdown, PhK is dephosphorylated by protein phosphatases 1 and 2, returning the α and β ... 1997). "The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognition". EMBO J. 16 ( ...
  https://en.wikipedia.org/wiki/Phosphorylase_kinase
*  Gene Report for G00001442 - Genes2Cognition Neuroscience Research Programme
Protein phosphatase 1 (PP1) catalytic subunits typically combine with other proteins that modulate their activity, direct them ... PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 ... While GADD34 inhibited PP1-catalyzed dephosphorylation of phosphorylase a, the GADD34-bound PP1 was an active eIF-2alpha ... a subunit of myosin phosphatase, plays a pivotal role in the regulation of myosin phosphatase activity. Here we have cloned a ...
  http://www.genes2cognition.org/db/Gene/G00001442
*  Principles of Biochemistry/Gluconeogenesis and Glycogenesis - Wikibooks, open books for an open world
This change increases phosphorylase activity up to 25% even in the absence of AMP, and enhances AMP activation further.[11] ... Glycogen phosphorylase has a pyridoxal phosphate (PLP, derived from Vitamin B6) at each catalytic site. Pyridoxal phosphate ... The pathway can begin in the mitochondria or cytoplasm, depending on the substrate being used. Many of the reactions are the ... This latter enzyme is itself activated by protein kinase A and deactivated by phosphoprotein phosphatase-1. Protein kinase A ...
  https://en.wikibooks.org/wiki/Principles_of_Biochemistry/Gluconeogenesis_and_Glycogenesis
*  Simultaneous stimulation of GABA and beta adrenergic receptors stabilizes isotypes of activated adenylyl cyclase heterocomplex ...
Two phosphatases associated with eNOS have one or the other phosphorylated site as specific substrate. When one phosphatase is ... synergistically stimulate phosphorylase kinase activity [31]. Furthermore, some molecules fine-tune their activities, depending ... might influence the Gαs GTPase activity. We determined the fractional occupancy of GTP in the catalytic site of Gαs and the ... GTPase activity was measured by the release of [32Pi] from [γ32P] GTP previously bound to Gαs. GTP hydrolytic activity was ...
  https://bmccellbiol.biomedcentral.com/articles/10.1186/1471-2121-5-25
*  Plus it
... phosphorylase a as substrate (Ingebritsen et al., 1983). For measurements of calcineurin activity, initial rate conditions were ... Differential subcellular localization of neural isoforms of the catalytic subunit of calmodulin-dependent protein phosphatase ( ... Nerve terminal phosphatase activities. Serine/threonine phosphatases have been extensively studied, but few studies have ... Ca2+-independent activity of phosphatase PP2A. The latter activity is also consistent with the low basal levels of ...
  http://www.jneurosci.org/content/21/20/7944
*  Cells | Free Full-Text | The Challenge of Producing Ubiquitinated Proteins for Structural Studies | HTML
... or affect its localization and activity. In order to understand the molecular mechanisms underlying the consequences of protein ... It has different effects on the target protein substrate, i.e., it can trigger the degradation of the protein in the proteasome ... used to explore the effect of ubiquitination on calmodulin-mediated regulation of phosphorylase kinase and protein phosphatase ... Ub was attached enzymatically to the catalytic site of the E2 UbcH5 by mutating the catalytic C85 into a lysine [9]. The ...
  http://www.mdpi.com/2073-4409/3/2/639/htm
*  Structural Biochemistry/Enzyme Regulation/Phosphorylation - Wikibooks, open books for an open world
These changes alter substrate binding and catalytic activity.. 2) A phosphoryl group can form 3 or more hydrogen bonds and can ... Phosphorylase activity is regulated by reversible phosphorylation. Phosphorylase a is the active form, while phosphorylase b is ... Protein phosphatases reverse the effects of kinases by catalyzing the removal of the phosphoryl group attached to proteins. The ... Regulatory subunits can also modulate catalytic subunit activity.. Regulating Activity: Intracellular concentration of cyclic ...
  https://en.wikibooks.org/wiki/Structural_Biochemistry/Enzyme_Regulation/Phosphorylation
*  PYGL - Wikipedia
This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 14. Activity ... which is phosphorylated by phosphorylase kinase and dephosphorylated by the phosphatase PP1, and the AMP site, which is ... "Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core". ... to bind the substrate glycogen. This cofactor is attached by a covalent Schiff base linkage to Lys-680 in the C-terminal domain ...
  https://en.wikipedia.org/wiki/PYGL
*  Structural Biochemistry/Switching - Wikibooks, open books for an open world
The other subunit, the regulatory subunit has no catalytic activity but binds to CTP. Therefore ATCase is composed of catalytic ... Donates ATP to glygogen phosphorylase which functions in glucose homeostasis and energy transduction. *Acetylation ... Kinases transfers the phosphoryl groups and Protein phosphatases reverses the process. However phosphorylation and ... effecting neighboring subunits by making it easier for substrate to bind.The increase of the concentration of ATP has two ...
  https://en.wikibooks.org/wiki/Structural_Biochemistry/Switching
*  Plus it
PP1c and PP2A activities were measured using 1 μm [32P] phosphorylase a as substrate (Ingebritsen et al., 1983) under initial ... Protein phosphatase 1 catalytic subunit (PP1c, Mr = 37,000) and protein phosphatase 2A (PP2A, complex of catalytic and ... Given that D1R- and D2R-dependent regulation of surface GABAA receptors requires the activity of protein phosphatases PP2A and ... Protein phosphatase signaling. Protein phosphatases PP1 and PP2A have been shown previously to associate with GABAA receptors ( ...
  http://www.jneurosci.org/content/30/8/2935
*  Phosphorylase | Article about Phosphorylase by The Free Dictionary
Find out information about Phosphorylase. An enzyme that catalyzes the formation of glucose-1-phosphate from glycogen and ... inorganic phosphate; it is widely distributed in animals, plants, and... Explanation of Phosphorylase ... the binding of an enzyme with a substrate, the allosteric regulation of enzyme activity, the dissociation of enzymes into ... Related to Phosphorylase: Glycogen phosphorylase, phosphorylase phosphatase, Purine nucleoside phosphorylase. phosphorylase. [ ...
  https://encyclopedia2.thefreedictionary.com/Phosphorylase
*  Frontiers | Computational Modeling of the Metabolic States Regulated by the Kinase Akt | Physiology
... two activities shown by both normal and cancer proliferating cells. Not surprisingly considering its relevance for cellular ... two activities shown by both normal and cancer proliferating cells. Not surprisingly considering its relevance for cellular ... as reported in literature in relation to the activity of the PI3K/Akt/mTOR. Some steps that link glycolysis and pentose ... as reported in literature in relation to the activity of the PI3K/Akt/mTOR. Some steps that link glycolysis and pentose ...
  https://www.frontiersin.org/articles/10.3389/fphys.2012.00418/full
*  Refresher
How enzymes work: active sites and catalytic mechanisms As with all proteins, the activity of enzymes depends on the precise ... substrate availability and in demand for their products. The activities of enzymes are regulated at different levels. ... effect until it is reversed by a specific protein phosphatase. Another, less obvious difference is that phosphorylation can ... phosphorylation inhibits glycogen synthase but activates glycogen phosphorylase, which degrades glycogen (slide 8.4.1). ...
  http://watcut.uwaterloo.ca/webnotes/Metabolism/Refresher.html
*  Transduction of the N-Terminal Fragments of MYPT1 Enhances Myofilament Ca2+ Sensitivity in an Intact Coronary Artery |...
Interaction of myosin phosphatase target subunit 1 with the catalytic subunit of type 1 protein phosphatase. Biochemistry. 1998 ... interaction between PP1c and MYPT1 increases the activity of PP1c toward MLC while inhibiting the activity toward phosphorylase ... enhancing substrate specificity toward myosin, and regulating enzymatic activity.4 The phosphorylation of T696 (in human MYPT1 ... The major myosin phosphatase in avian smooth muscle is a novel form of protein phosphatase-1. Eur J Biochem. 1992; 210: 1023- ...
  http://atvb.ahajournals.org/content/24/3/464
*  Glycogen synthase - Wikipedia
... structural similarities to glycogen phosphorylase at the catalytic and substrate binding site suggest that the mechanism for ... The role of muscle glycogen is as a reserve to provide energy during bursts of activity. Meanwhile, the muscle isozyme plays a ... In a recent study of transgenic mice, an overexpression of glycogen synthase and an overexpression of phosphatase both resulted ... Much research has been done on glycogen degradation through studying the structure and function of glycogen phosphorylase, the ...
  https://en.wikipedia.org/wiki/Glycogen_synthase
*  PROTEIN KINASE AND PHOSPHATASE INHIBITORS - Patent application
... is the natural substrate M1 whose phosphorylation rate is accelerated by interactions with the catalytic residues. The results ... protein phosphatase activity is compared to the level of activity from the protein kinase or protein phosphatase without the ... inhibit glycogen phosphorylase for Type II diabetes, HIV reverse transcriptase, or thromboxane synthase). Thus, these compounds ... protein phosphatase activity in the subject. [0126]Protein phosphatase inhibitors may be used in various therapeutic techniques ...
  http://www.patentsencyclopedia.com/app/20110028474