JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
SpAvt3p of Schizosaccharomyces pombe, a homologue of these vacuolar amino acid transporters, has been indicated to be involved ... Adaptors also package transmembrane protein cargo, such as receptors, and can interact with each other and with other ... Increased protein abundance should be biochemically confirmed. A method for the rapid extraction of yeast proteins in a form ... Functional Expression and Characterization of Schizosaccharomyces pombe Avt3p as a Vacuolar Amino Acid Exporter in ...https://www.jove.com/visualize/abstract/26083598/functional-expression-characterization-schizosaccharomyces-pombe
sec18 - Vesicular-fusion protein sec18 - Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) - sec18 gene &...
Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack ... Schizosaccharomyces pombe. Schizosaccharomyces pombe: entries and gene names *SIMILARITY comments. Index of protein domains and ... sp,Q9P7Q4,SEC18_SCHPO Vesicular-fusion protein sec18 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=sec18 PE=1 SV=1 ... Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack ...http://www.uniprot.org/uniprot/Q9P7Q4
Supplement to Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome...
Although present in most eukaryotes, including the filamentous euascomycetes and Schizosaccharomyces pombe, there are no ... "cargo" protein selection (8). Genes coding for COPII subunits are evolutionarily well conserved and most have single orthologs ... We also noticed that K. polysporus has a gene (Kpol_520.25) coding for a protein in the Argonaute family. Argonaute proteins ... Protein complexes. Protein complexes and genes coding for their components tend to be lost and gained relatively rarely during ...http://www.pnas.org/content/suppl/2007/04/25/0608218104.DC1
we provide more evidence to clarify the molecular mechanism of HBV inhibition mediated by TGF-b1 | Cannabinoid receptor...
Materials and Methods Strains, Media, Genetic and Molecular Biology Methods The Schizosaccharomyces pombe strains used in this ... as an essential protein that interacted with the F-box protein Pof6, and concluded that Sip1 was an endocytic 1260907-17-2 web ... Further, we identified Bgs1, required for cell wall synthesis, as the new cargo of AP-1-dependent trafficking. ... Strain HM123 cells were mutagenized with 300 mm nitrosoguanidine for 60 min, as described by Moreno et AP-1 Accessory Protein ...http://www.cannabinoid-receptor.com/2017/06/19/we-provide-more-evidence-to-clarify-the-molecular-mechanism-of-hbv-inhibition-mediated-by-tgf-b1/
Myosin V spatially regulates microtubule dynamics and promotes the ubiquitin-dependent degradation of the fission yeast CLIP...
Mitchison, J. M. and Nurse, P. (1985). Growth in cell length in the fission yeast Schizosaccharomyces pombe. J. Cell Sci. 75, ... Class V myosins are processive actin-associated motor proteins that dimerise to transport molecular cargos throughout the cell ... The fission yeast Schizosaccharomyces pombe is an excellent and simple model organism in which to study how cell shape and ... Moreno, S., Klar, A. and Nurse, P. (1991). Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods ...http://jcs.biologists.org/content/122/21/3862
Opposing kinesin complexes queue at plus tips to ensure microtubule catastrophe at cell ends | EMBO Reports
des Georges A, Katsuki M, Drummond DR, Osei M, Cross RA, Amos LA (2008) Mal3, the Schizosaccharomyces pombe homolog of EB1, ... repeat protein Tea1 and SH3‐domain protein Tea4 to the plus ends of iMTs by association with Tea2 (Kinesin‐7) and their ... but not its cargo (Tea1, Tea3 and Tea4), at the MT plus end also prevents premature MT catastrophe in the cytoplasm , at ... Sandblad L, Busch KE, Tittmann P, Gross H, Brunner D, Hoenger A (2006) The Schizosaccharomyces pombe EB1 homolog Mal3p binds ...http://embor.embopress.org/content/early/2018/09/11/embr.201846196
WikiGenes - RAB11A - RAB11A, member RAS oncogene family
Identification and characterization of a human homolog of the Schizosaccharomyces pombe ras-like gene YPT-3. Drivas, G.T., Shih ... Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein. Lindsay, A.J., Hendrick, A.G., Cantalupo, G., Senic- ... Interactions of myosin vb with rab11 family members and cargoes traversing the plasma membrane recycling system. Lapierre, L.A ... We therefore propose that RCP functions primarily as a Rab11 binding protein that regulates protein sorting in tubular ...http://www.wikigenes.org/e/gene/e/8766.html
Protocols and Video Articles Authored by Manfred J. Schmitt
Schizosaccharomyces Pombe: a Novel Transport Vehicle of Functional DNA and MRNA into Mammalian Antigen-presenting Cells Vaccine ... Peptide maps of the purified HuV coat protein and the 81-kDa major capsid protein from K1 killer viruses of Saccharomyces ... Cargo Binding Promotes KDEL Receptor Clustering at the Mammalian Cell Surface Scientific Reports. , Pubmed ID: 27353000 ... Schizosaccharomyces pombe, Kluyveromyces lactis and Pichia pastoris) and human dendritic cells as well as the involvement of ...https://www.jove.com/author/Manfred+J._Schmitt
The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique...
Although the import cargoes of the fission and budding yeast importin-α proteins remain largely unknown and may not be the same ... pombe importin-α proteins have some overlapping functions.. However, neither of the S. pombe importin-α1 proteins can fully ... The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique ... The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique ...http://www.genetics.org/content/171/1/7
Cell proliferation, tumorogenicity, and apoptosis sub-cluster 33
The fission yeast Schizosaccharomyces pombe is a rod-shaped cell that exhibits polarized cell growth at cell tips. MT plus ends ... Levels and activities of proteins are regulated in many ways, one of which is ubiquitin-mediated protein degradation. E3 ... Microtubule motors drive the movement of many different cargoes in eukaryotic cells. A combination of in vitro and in vivo ... Translationally controlled tumor-associated protein (TCTP) is a ubiquitous and highly conserved protein implicated in cancers. ...http://www.biomedsearch.com/cluster/2/Cell-proliferation-tumorogenicity-and-apoptosis/sub-33-p8.html
ESCRTs are everywhere | The EMBO Journal
... associated protein ALIX. In yeast and animals, ubiquitinated cargo is organized into a flat clathrin‐coated domain by ESCRT‐0 ... a role shared from the Crenarchaea to Schizosaccharomyces pombe to mammals (Samson & Bell, 2009) (Table 1). Thus, the primal ... Ubiquitinated Cos proteins provide a sorting signal in trans to non‐ubiquitinated cargo destined for ILVs and so facilitate ... Morita E, Sandrin V, Chung HY, Morham SG, Gygi S, Rodesch CK, Sundquist WI (2007) Human ESCRT and ALIX proteins interact with ...http://emboj.embopress.org/content/early/2015/08/25/embj.201592484
Dynein Modifiers in C. elegans: Light Chains Suppress Conditional Heavy Chain Mutants
Many of the proteins that specifically influence dynein localized to subcellular sites where the dynein heavy chain has been ... An essential motor protein complex called cytoplasmic dynein powers many aspects of microtubule-dependent transport, but it is ... Author SummaryMicrotubules and microtubule-dependent motor proteins segregate chromosomes during mitosis and also promote ... and Schizosaccharomyces pombe, NP_001018285.. The NCBI accession numbers for the Drosophila and human DYLT-1 and DYRB-1 protein ...http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.0030128
Dynein - Wikipedia
However, in the fission yeast Schizosaccharomyces pombe, when chiasmata are absent, dynein promotes segregation. Dhc1, the ... another multisubunit protein that is essential for mitosis, and a cargo adaptor. The tri-complex, which includes dynein, ... Davis L, Smith GR (June 2005). "Dynein promotes achiasmate segregation in Schizosaccharomyces pombe". Genetics. 170 (2): 581-90 ... dynactin and a cargo adaptor, is ultra-processive and can walk long distances without detaching in order to reach the cargo's ...https://en.wikipedia.org/wiki/Dynein
Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris |...
... recombinant Schizosaccharomyces pombe and recently also in recombinant P. pastoris[50, 51]. Apart from the more apparent ... since the targeting of membrane protein cargo to the cell surface requires early raft association already in the ER . As a ... ER membrane protein involved in translocation of soluble secretory proteins and insertion of membrane proteins into the ER ... Wsc4p is a component of the ER and plays a role in the translocation of soluble proteins as well as the insertion of proteins ...https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-10-93
BICDL1 Gene - GeneCards | BICL1 Protein | BICL1 Antibody
Protein Coding), BICD Family Like Cargo Adaptor 1, including: function, proteins, disorders, pathways, orthologs, and ... fission yeast (Schizosaccharomyces pombe). *green algae (Chlamydomonas reinhardtii). *honey bee (Apis mellifera) ... Protein Symbol:. Q6ZP65-BICL1_HUMAN. Recommended name:. BICD family-like cargo adapter 1 Protein Accession:. Q6ZP65. Secondary ... Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners , mRNA ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=BICDL1
Reconstitution of dynein transport to the microtubule plus end by kinesin | eLife
In vitro reconstitution reveals how a system of molecules works together to transport the motor protein dynein to the start of ... resembling the behavior of related proteins from Schizosaccharomyces pombe (Bieling et al., 2007; Table 1). A pair-wise mixture ... Cytoplasmic dynein, a large and complex AAA+ motor protein (Carter, 2013), uses the energy from ATP hydrolysis to move cargoes ... Protein concentrations were determined by comparisons with standards using Bradford protein assays. All protein concentrations ...https://elifesciences.org/articles/02641
Table of Contents | Journal of Cell Science
The Schizosaccharomyces pombe pyp1 protein tyrosine phosphatase negatively regulates nutrient monitoring pathways P. Dal Santo ... For the majority of cargo proteins secreted by unconventional means the precise molecular details of secretion routes have ... The polymorphic proteins TgrB1 and TgrC1 function as a ligand-receptor pair in Dictyostelium allorecognition. Shigenori Hirose ... The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking. Jing ...http://jcs.biologists.org/content/109/7
KLC1 Antibody (Center) - Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) WB, IHC-P, FC, E - Buy Now! |Abgent
Schizosaccharomyces pombe. Caenorhabditis elegans. Cre/lox System. Cat. Bacteria. Avian. Amphibian. All Species. S. Aureus. ZA ... The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins ... The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. ... The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. ...http://www.abgent.com/products/AP8637c-KLC1-Antibody-Center
TNPO1 Antibody (N-term) - Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) WB, IHC-P, FC, E - Buy Now! |Abgent
Schizosaccharomyces pombe. Caenorhabditis elegans. Cre/lox System. Cat. Bacteria. Avian. Amphibian. All Species. S. Aureus. ZA ... The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins ... Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/ ... With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel ...http://www.abgent.com/products/AP1934e-TNPO1-Antibody-N-term
SEC24D Antibody (monoclonal) (M04) - Mouse monoclonal antibody raised against a partial recombinant SEC24D. WB - Buy Now! ...
Schizosaccharomyces pombe. Caenorhabditis elegans. Cre/lox System. Cat. Bacteria. Avian. Amphibian. All Species. S. Aureus. ZA ... Protein transport protein Sec24D, SEC24-related protein D, SEC24D, KIAA0755. Target/Specificity SEC24D (NP_055637, 935 a.a. ~ ... This gene product is implicated in the shaping of the vesicle, and also in cargo selection and concentration. ... The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for ...http://www.abgent.com/products/AT3806a-SEC24D-Antibody-monoclonal-M04
Transgenesis upgrades for Drosophila melanogaster | Development
Pioneered in the yeast Schizosaccharomyces pombe (Thomason et al., 2001) and in mouse (Belteki et al., 2003), a modification of ... and a fluorescent protein, such as enhanced green fluorescent protein (EGFP) (Berghammer et al., 1999; Horn et al., 2000). A ... Transposons are generally characterized by a low cargo capacity, limiting the amount of DNA that can be integrated and ... Thomason, L. C., Calendar, R. and Ow, D. W. (2001). Gene insertion and replacement in Schizosaccharomyces pombe mediated by the ...http://dev.biologists.org/content/134/20/3571
AP2A2 Antibody (Center) - Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) WB, IHC-P, FC, E - Buy Now! |Abgent
Schizosaccharomyces pombe. Caenorhabditis elegans. Cre/lox System. Cat. Bacteria. Avian. Amphibian. All Species. S. Aureus. ZA ... Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 ... Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport ... AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 ...http://www.abgent.com/products/AP8551c-AP2A2-Antibody-Center
ILF3 Gene - GeneCards | ILF3 Protein | ILF3 Antibody
Protein Coding), Interleukin Enhancer Binding Factor 3, including: function, proteins, disorders, pathways, orthologs, and ... fission yeast (Schizosaccharomyces pombe). *fruit fly (Drosophila melanogaster). *green algae (Chlamydomonas reinhardtii) ... Nucleocytoplasmic shuttling of JAZ, a new cargo protein for exportin-5. (PMID: 15254228) Chen T … Macara IG (Molecular and ... Protein Symbol:. Q12906-ILF3_HUMAN. Recommended name:. Interleukin enhancer-binding factor 3 Protein Accession:. Q12906. ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=ILF3
The cell polarity proteins Boi1p and Boi2p stimulate vesicle fusion at the plasma membrane of yeast cells | Journal of Cell...
Cdc24p and the scaffold protein Bem1p (Bender et al., 1996). As Pob1p, the Boi1/2p homologue in Schizosaccharomyces pombe, was ... 2012). Myosin-V is activated by binding secretory cargo and released in coordination with rab/exocyst function. Dev. Cell. 23, ... Protein-protein interaction analysis of the yeast type II phosphatase Ptc1p and its adaptor protein Nbp2p. J. Cell Sci. 124, 35 ... 2012). Detecting protein-protein interactions with the split-ubiquitin sensor. Methods Mol. Biol. 786, 115-130. doi:10.1007/978 ...http://jcs.biologists.org/content/130/18/2996
ARFIP2 Antibody (monoclonal) (M01) - Mouse monoclonal antibody raised against a partial recombinant ARFIP2. WB, E - Buy Now! ...
Schizosaccharomyces pombe. Caenorhabditis elegans. Cre/lox System. Cat. Bacteria. Avian. Amphibian. All Species. S. Aureus. ZA ... The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins ... 1.Recruitment of arfaptins to the trans-Golgi network by PI(4)P and their involvement in cargo export.Cruz-Garcia D, Ortega- ... Arfaptin-2, ADP-ribosylation factor-interacting protein 2, Partner of RAC1, Protein POR1, ARFIP2, POR1. ...http://www.abgent.com/products/AT1178a-ARFIP2-Antibody-monoclonal-M01