*  Anti-Myosin Light Chain 2 antibody (ab48003) | Abcam
Rabbit polyclonal Myosin Light Chain 2 antibody validated for WB, ELISA, IHC, ICC/IF and tested in Rat. Referenced in 8 ... Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody. *Regulatory light chain of myosin antibody ... Lane 1 : Vascular myosin (rat aorta), loaded with 35 µg of lysate. Lane 2 : Cardiac myosin (mouse heart, loaded with 35 µg of ... Anti-Myosin Light Chain 2 antibody. See all Myosin Light Chain 2 primary antibodies. ...
  http://www.abcam.com/myosin-light-chain-2-antibody-ab48003.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... The potential of exploiting human induced pluripotent stem cell (hiPSC) technology to generate cardiac muscle "in a dish" for ... Identification and characterization of the properties and regulation of different connexin and pannexin isoforms as gap ... offering us new opportunities to model very early stages of human cardiac development not otherwise accessible. In contrast to ...
  https://www.jove.com/visualize/abstract/19549873/paleocene-emergence-elephant-relatives-rapid-radiation-african
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... The potential of exploiting human induced pluripotent stem cell (hiPSC) technology to generate cardiac muscle "in a dish" for ... offering us new opportunities to model very early stages of human cardiac development not otherwise accessible. In contrast to ... aggregation or the addition of Activin A or BMP4 and robustly generates cultures of cells that are highly positive for cardiac ...
  https://www.jove.com/visualize/abstract/22132178/generation-induced-pluripotent-stem-cells-from-cd34-cells-across
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... Notably, the C-terminal domain PHD of RBP2 is absent in the smaller RBP2 isoform4. It is conceivable that the small isoform of ... We observed that GF14 isoforms of the non-? group were more effective than ? group isoforms in the interaction with the H+- ... 14-3-3s exist as a number of isoforms that in Arabidopsis identifies two major groups named ? and non-?. Although isoform ...
  https://www.jove.com/visualize/abstract/24603559/specificity-non-isoforms-arabidopsis-14-3-3-proteins-towards-h-atpase
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... A sarcomere unit contains actin and myosin filaments that are spaced by the Z discs and titin protein. Mechanical function of ... The potential of exploiting human induced pluripotent stem cell (hiPSC) technology to generate cardiac muscle "in a dish" for ... Any factor that affects muscle contraction (such as interaction between actin and myosin filaments, homeostasis of calcium, ATP ...
  https://www.jove.com/visualize/abstract/23717655/local-applications-myostatin-sirna-with-atelocollagen-increase
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... The potential of exploiting human induced pluripotent stem cell (hiPSC) technology to generate cardiac muscle "in a dish" for ... In addition, further observations revealed that specific stem cells, such as cardiac stem cells, mesenchymal stem cells and ... Here, we present the materials and methods to reliably deliver noncontractile cells into the left ventricular wall of ...
  https://www.jove.com/visualize/abstract/24098678/long-term-evaluation-impact-autologous-peripheral-blood-stem-cell
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... The potential of exploiting human induced pluripotent stem cell (hiPSC) technology to generate cardiac muscle "in a dish" for ... We present our established models for morphogenetic signaling, craniofacial, cardiac, vascular integrity, renal function, and ... offering us new opportunities to model very early stages of human cardiac development not otherwise accessible. In contrast to ...
  https://www.jove.com/visualize/abstract/23050008/gene-wide-characterization-common-quantitative-trait-loci-for-abcb1
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... Rare events in cardiac myocytes such as proliferation and apoptosis require an accurate identification of cardiac myocyte ... The production of corresponding protein isoforms is validated by immunoblotting assays. The method of detecting splice isoforms ... Using isoform-specific primer sets, the alternative splicing of interested mRNAs are analyzed by quantitative RT-PCR. ...
  https://www.jove.com/visualize/abstract/20041118/conserved-usage-of-alternative-5-untranslated-exons-of-the-gata4-gene
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... Rare events in cardiac myocytes such as proliferation and apoptosis require an accurate identification of cardiac myocyte ... We show how to easily dissect hearts from neonates, dissociate the cardiac tissue and enrich cardiomyocytes from the cardiac ... Anti-apoptotic BCL-2 family proteins (e.g., BCL-2 related gene, long isoform, BCL-xL; myeloid cell leukemia 1, MCL-1) regulate ...
  https://www.jove.com/visualize/abstract/21088907/inhibitory-effect-hepatocyte-growth-factor-on-cardiomyocytes
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... Computational simulations of ventricular activation maps and pseudo-ECGs in sinus rhythm and ventricular tachycardia indicated ... Ventricular geometry of a patient heart was extracted, via semiautomatic segmentation, from an in vivo computed tomography (CT ... In cardiac myocytes a complex network of membrane tubules - the transverse-axial tubule system (TATS) - controls deep ...
  https://www.jove.com/visualize/abstract/25265017/multivariate-longitudinal-shape-analysis-human-lateral-ventricles
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v) and myosin regulatory light chain 2, atrial isoform ... The potential of exploiting human induced pluripotent stem cell (hiPSC) technology to generate cardiac muscle "in a dish" for ... We further extend our basic protocol to cultivate hPSCs on defined extracellular proteins such as the laminin isoform 521 (LN- ... offering us new opportunities to model very early stages of human cardiac development not otherwise accessible. In contrast to ...
  https://www.jove.com/visualize/abstract/23226362/contribution-stochastic-partitioning-at-human-embryonic-stem-cell
*  MYL3 - Wikipedia
Myosin essential light chain (ELC), ventricular/cardiac isoform is a protein that in humans is encoded by the MYL3 gene. This ... Ventricular ELC is part of the myosin molecule and is important in modulating cardiac muscle contraction. Cardiac, ventricular ... cardiac ventricular/slow skeletal ELC isoform is distinct from that expressed in fast skeletal muscle (MYL1) and cardiac atrial ... two myosin alkali light-chain genes encoding the ventricular/slow skeletal muscle isoform and the atrial/fetal muscle isoform ( ...
  https://en.wikipedia.org/wiki/MYL3
*  MYL2 - Wikipedia
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2) also known as the regulatory light chain of myosin ... Ventricular myosin light chain-2 (MLC-2v) refers to the ventricular cardiac muscle form of myosin light chain 2 (Myl2). MLC-2v ... "Cardiac myosin light chain kinase is necessary for myosin regulatory light chain phosphorylation and cardiac performance in ... This cardiac ventricular RLC isoform is distinct from that expressed in skeletal muscle (MYLPF), smooth muscle (MYL12B) and ...
  https://en.wikipedia.org/wiki/MYL2
*  MYH6 - Wikipedia
This isoform is distinct from the ventricular/slow myosin heavy chain isoform, MYH7, referred to as MHC-β. MHC-α isoform is ... cardiac ventricles predominantly express the MHC-β isoform and cardiac atria predominantly express the MHC-α isoform. The two ... MHC-α isoform is abundantly expressed in both cardiac atria and cardiac ventricles during embryonic development. Following ... 4kb downstream of the MYH7 gene encoding the other major cardiac muscle isoform of myosin heavy chain, MHC-β. MHC-α is a ...
  https://en.wikipedia.org/wiki/MYH6
*  MYL7 - Wikipedia
ALC-2 is an EF hand protein that binds to the neck region of alpha myosin heavy chain. ALC-2 and the ventricular isoform, VLC-2 ... "Cardiac myosin light chain kinase is necessary for myosin regulatory light chain phosphorylation and cardiac performance in ... A study in which the ventricular isoform of regulatory light chain was overexpressed to replace the ALC-2 in cardiac atria was ... Cummins P (July 1982). "Transitions in human atrial and ventricular myosin light-chain isoenzymes in response to cardiac- ...
  https://en.wikipedia.org/wiki/MYL7
*  MYL4 - Wikipedia
Morano I, Haase H (May 1997). "Different actin affinities of human cardiac essential myosin light chain isoforms". FEBS Letters ... Shift in atrial myosin heavy chains and in ventricular myosin light chains". European Heart Journal. 5 Suppl F: 85-93. doi: ... ALC-1 is expressed in fetal cardiac ventricular and fetal skeletal muscle, as well as fetal and adult cardiac atrial tissue. ... ALC-1 is expressed in fetal cardiac ventricular and fetal skeletal muscle, as well as fetal and adult cardiac atrial tissue. ...
  https://en.wikipedia.org/wiki/MYL4
*  Myotonin-protein kinase - Wikipedia
... and cardiac muscles. This kinase exists both as a membrane-associated and as a soluble form in human left ventricular samples. ... Data obtained by using antibodies that detect specific isoforms of DMPK indicate that the most abundant isoform of DMPK is an ... Myosin phosphatase is an enzyme that plays a role in muscle tensing (contraction) and relaxation. Dystrophia myotonica protein ... For example, myotonic dystrophy protein kinase has been shown to turn off (inhibit) part of a muscle protein called myosin ...
  https://en.wikipedia.org/wiki/Myotonin-protein_kinase
*  TPM2 - Wikipedia
... along with cardiac abnormalities, suggesting that the normal distribution of tropomyosin isoforms is critical to normal cardiac ... myosin ATPase activity, and the cardiac response to stress. A decrease in β-tropomyosin in patients with heart failure was ... Transgenic hearts showed a significant delay in relaxation time as well as a decrease in the maximum rate of left ventricular ... Yin Z, Ren J, Guo W (Jan 2015). "Sarcomeric protein isoform transitions in cardiac muscle: a journey to heart failure". ...
  https://en.wikipedia.org/wiki/TPM2
*  MYH10 - Wikipedia
NM-IIB is the only non-muscle myosin II isoform expressed in adult cardiac muscle (both IIa and IIB are expressed in skeletal ... Feature observed in NM-IIB knockouts was an increase in the transverse diameters of cardiomyocytes, ventricular septal defects ... Non-muscle myosins are expressed in a wide variety of tissues, but NM-IIB is the only non-muscle myosin II isoform expressed in ... Myosin-10 also known as myosin heavy chain 10 or non-muscle myosin IIB (NM-IIB) is a protein that in humans is encoded by the ...
  https://en.wikipedia.org/wiki/MYH10
*  Actin - Wikipedia
In general, alpha actins are found in muscle (α-skeletal, α-aortic smooth, α-cardiac), whereas beta and gamma isoforms are ... Kabaeva Z (2002). Genetic analysis in hypertrophic cardiomyopathy: missense mutations in the ventricular myosin regulatory ... to be a track for cargo transport myosins (nonconventional myosins) such as myosin V and VI. Nonconventional myosins use ATP ... This arrangement allows myosin V to be an effective motor for the export of cargos, and myosin VI to be an effective motor for ...
  https://en.wikipedia.org/wiki/Actin
*  TNNT2 - Wikipedia
"Clinical manifestations of hypertrophic cardiomyopathy with mutations in the cardiac beta-myosin heavy chain gene or cardiac ... The TNNT2 gene is located at 1q32 in the human chromosomal genome, encoding the cardiac muscle isoform of troponin T (cTnT). ... "A new mutation of the cardiac troponin T gene causing familial hypertrophic cardiomyopathy without left ventricular hypertrophy ... The N-terminal region of TnT is alternatively spliced, accounting for multiple isoforms observed in cardiac muscle. As part of ...
  https://en.wikipedia.org/wiki/TNNT2
*  ACTC1 - Wikipedia
This isoform differs from the alpha actin that is expressed in skeletal muscle, ACTA1. Alpha cardiac actin is the major protein ... "Mutation in the alpha-cardiac actin gene associated with apical hypertrophic cardiomyopathy, left ventricular non-compaction, ... Myosin binding increases the flexibility of actin, and cross-linking studies have shown that myosin subfragment-1 binds to ... Cardiac alpha actin is a 42.0 kDa protein composed of 377 amino acids. Cardiac alpha actin is a filamentous protein extending ...
  https://en.wikipedia.org/wiki/ACTC1
*  Myosin binding protein C, cardiac - Wikipedia
"Phosphorylation switches specific for the cardiac isoform of myosin binding protein-C: a modulator of cardiac contraction?". ... "Role of cardiac myosin binding protein C in sustaining left ventricular systolic stiffening". Circulation Research. 94 (9): ... "Phosphorylation switches specific for the cardiac isoform of myosin binding protein-C: a modulator of cardiac contraction?". ... "Phosphorylation switches specific for the cardiac isoform of myosin binding protein-C: a modulator of cardiac contraction?". ...
  https://en.wikipedia.org/wiki/Myosin_binding_protein_C,_cardiac
*  PRKCE - Wikipedia
PKCε is an isoform of the large PKC family of protein kinases that play many roles in different tissues. In cardiac muscle ... Activation of PKCε by εRACK prior to ischemia was also found to phosphorylate Ventricular myosin light chain-2, however the ... The epsilon isoform of PKC differs from other isoforms by the position of the C2, pseudosubstrate, and C1 domains; various ... PKCε binds and phosphorylates cardiac troponin I (cTnI) and cardiac troponin T (cTnT) in complex with troponin C (cTnC); ...
  https://en.wikipedia.org/wiki/PRKCE
*  Troponin - Wikipedia
No cardiac specific isoforms are known for human TnC. TnC in human cardiac muscle tissue is presented by an isoform typical for ... April 2001). "Cardiac troponin and beta-type myosin heavy chain concentrations in patients with polymyositis or dermatomyositis ... ventricular hypertrophy, peripartum cardiomyopathy, Takotsubo cardiomyopathy, or infiltrative disorders such as cardiac ... Only one tissue-specific isoform of TnI is described for cardiac muscle tissue (cTnI), whereas the existence of several cardiac ...
  https://en.wikipedia.org/wiki/Troponin