*  New Imaging Technique Reveals Heart Damage Earlier | National Institute of Biomedical Imaging and Bioengineering
Researchers funded by the National Institutes of Health have developed a high-resolution MRI technique to visualize disruptions ... group is currently working to further optimize the creatine CEST MRI technique so that it can be implemented in cardiac imaging ... "It also provides a safer alternative to current imaging techniques, which require the injection of a contrast dye or ... In their study, the researchers adopted a known MRI technique called Chemical Exchange Saturation Transfer (CEST)-used to image ...
  https://www.nibib.nih.gov/news-events/newsroom/new-imaging-technique-reveals-heart-damage-earlier
*  Magnetic Resonance Imaging Systems Market - Industry Report 2024
Magnetic Resonance Imaging Systems Market share anticipated to maintain handsome growth in coming years; India MRI systems ... The fact that these imaging techniques are extremely sensitive to abnormal and normal patterns of myelination, MR images are ... Allegedly, this unique technique gives clinicians the flexibility to use MR images retrospectively, resulting in fewer rescans ... enabling simplified cardiac imaging workflows, faster contrast scans, and even allowing MR scans for lungs. Toshiba's ...
  https://www.fractovia.org/news/industry-research-report/magnetic-resonance-imaging-mri-systems-market
*  Search Subject Areas | Preprints
Then, the conventional spheroid formation techniques such as bioreactor flasks, liquid overlay and hanging droplet technique ... For the analysis of orthogonal moments hundred random melanoma images and hundred non-melanoma images have been taken into ... We propose a lightweight and model-free approach for the online detection of cardiac anomalies such as ectopic beats in ECG or ... Online: 13 July 2018 (11:06:05 CEST) Show abstract, Download PDF, Supplementary Files, Share Share this article with. ...
  https://www.preprints.org/subject/browse/engineering/biomedical_chemical_engineering
*  Magnetic Resonance Reporter Gene Imaging
C) MR images. (D) A CEST signal intensity-difference map overlaid on anatomical images of LRP-expressing (left) and control ( ... The major benefits of reporter gene techniques are that cells must be viable to produce an imaging signal, and that imaging ... Current perspectives on imaging cardiac stem cell therapy. J Nucl Med. 2010;51(Suppl 1):128S-36S ... Click on the image to enlarge.). Another enzymatic reporter gene technique for MRS utilizes b-galactosidase. Here, 19F MRS ...
  http://www.thno.org/v02p0403.htm
*  All Early View Articles - separationsNOW.com
CEST MRI exploits a specific MR frequency (chemical shift) of a molecule while generating an image with good spatial resolution ... using standard MRI techniques, combining the specificity of MRS with the spatial resolution of MRI. Many CEST MRI acquisition ... Abstract Raman microscopy is a molecular technique that is able to detect compounds that are present in the samples at minor/ ... Abstract Periplocin is a cardiac glycoside and has been used widely in the clinic for its cardiotonic, anti‐inflammatory and ...
  http://www.separationsnow.com/view/earlyview.html?page=14
*  Search Subject Areas | Preprints
Surface etching techniques delivered a thicker native TiO2 layer, being both grain refinement and surface etching techniques ... and the extent of influence of materials on cardiac embolism. Clinically, cement leakage results in life-threatening cardiac ... Laser ablation technique was used to define the size and structure of the biosensor. Cost-effective roll-to-roll manufacturing ... Results from image studies demonstrated that, in the group without bone graft, all studied femurs exhibited either nonunion or ...
  https://www.preprints.org/subject/browse/materials_science/biomaterials
*  Radiology Research Forum | CAI2R
... based image reconstruction techniques used for accelerating data acquisition in cardiac MRI. We will introduce techniques that ... The TRUST MRI technique applies the spin labeling principle on the venous side and acquires control and labeled images, the ... Abstract: Recently, Chemical Exchange Saturation Transfer (CEST) has emerged as an attractive MRI contrast mechanism. In CEST, ... Challenges in free-breathing 3D cardiac magnetic resonance cine imaging Abstract: Cardiac cine imaging is an important part of ...
  http://cai2r.net/training/radiology-research-forum
*  ISMRM Scientific Meeting & Exhibition - Siemens Healthineers Česká republika
Helix angle (HA) healthy statistical average technique for HA quantification in vivo cardiac diffusion tensor imaging. Peter ... Emerging Body MR Techniques Accelerated diffusion weighted imaging in the liver with blipped CAIPIRINHA based simultaneous ... Intervertebral Disc CEST Imaging with Improved Reliability Using Reduced-FOV TSE. Ning Jin ... Helix angle (HA) healthy statistical average technique for HA quantification in vivo cardiac diffusion tensor imaging. Thorsten ...
  https://www.healthcare.siemens.cz/magnetic-resonance-imaging/magnetom-world/publications/ismrm2/ismrm-2013
*  Clavien, Pierre-Alain<...
Tschuor, Christoph; et al; Clavien, Pierre-Alain (2016). Techniques of liver parenchyma dissection. In: Clavien, Pierre-Alain; ... This list was generated on Tue Sep 25 04:05:00 2018 CEST. ... A novel technique in mouse liver transplantation. Transplant ... Cosmesis and body image in patients undergoing single-port versus conventional laparoscopic cholecystectomy: a multicenter ... HOPE for human liver grafts obtained from donors after cardiac death. Journal of Hepatology, 60(4):765-772. ...
  https://www.zora.uzh.ch/view/authors_for_linking_in_citation/Clavien=3APierre-Alain=3A=3A.html
*  Projects - BIOQIC
For optimizing diagnostic yield, however, the combination of various imaging techniques is desired.. Objective: Based on the ... Background: MRI yields both high-resolution 3D coronary angiography images and respiratory and cardiac motion information, ... Such sensors can generate a contrast which is switched on and off through the MRI pulse sequence (hyper-CEST) and is - other ... validation of the new technique in this phantom and iv) application of the method in healthy volunteers and patients. Once ...
  https://bioqic.de/projects/
*  Most recent papers with the keyword Trends mri | Read by QxMD
However, artifacts are prone to develop in breast CEST imaging as a result of strong lipid signals. The aims of the study are ( ... as well as arterial blood volume imaging techniques... ... heart or cardiac or cardio*) AND arrest -"American Heart ... After a brief overview of the technique's fundamentals, this article will review new trends and variants in ASL including ... magnetic resonance imaging, ultrasound imaging or multimodal imaging, as well as for various therapeutic approaches including ...
  https://www.readbyqxmd.com/keyword/120487
*  ISMRM 25th Annual Meeting Session Page
The aim of this study was to identify the analysis technique with the optimum repeatability for quantifying APT CEST imaging ... There is an unmet need for imaging techniques that probe the pathological substrate of CI at a clinically relevant field ... Human cardiac creatine by CrCEST: potential improved fitting methods Jane Ellis, Mabel Li, Michael Chappell, Mathew Robson, ... We compare the results of CEST imaging to Single Voxel Spectroscopy and high-resolution T2-weighted imaging. ...
  https://www.ismrm.org/17/program_files/EP06.htm
*  Protocols and Video Articles Authored by Peter R. Luijten (Translated to Spanish)
Techniques and Applications of Skeletal Muscle Diffusion Tensor Imaging: A Review Journal of Magnetic Resonance Imaging : JMRI ... technique for selective peripheral nerve imaging, called "subtraction of unidirectionally encoded images for suppression of ... Influence of Cardiac Motion on Diffusion-weighted Magnetic Resonance Imaging of the Liver Magma (New York, N.Y.). Oct, 2009 , ... Oct, 2013 , Pubmed ID: 23559550 Chemical exchange saturation transfer (CEST) can offer information about protons associated ...
  https://www.jove.com/author/Peter+R._Luijten?language=Spanish
*  have varying ketoconazole ibuprofen interaction
Another accurate ketoconazole ibuprofen interaction very sensitive imaging tech- nique for diagnosing and intreaction a large ... These techniques ketoonazole used to evaluate cardiac ibuprofe and ketoconaole of ketoconazole ibuprofen interaction aorta and ... in addition to ensuring that cest quoi duphaston skin receives meticulous attention and cleansing. ... В Teachreinforce appropriate technique for incentive spirome- try. Subsequently, Gian Poggio and David Ferster showed that ...
  http://luxbar-starway.ru/cheap-drugs-in-india/ketoconazole-ibuprofen-interaction.html
*  Publications for Alkadhi, H - Zurich Open Repository and Archive
Cardiac hybrid imaging. European Journal of Nuclear Medicine and Molecular Imaging, 41(S1):91-103. ... a comparison of radionuclide and CT imaging techniques. European Radiology, 24(7):1455-1465. ... This list was generated on Sat Aug 18 12:36:47 2018 CEST. ... surgical technique, and outcome. Annals of Thoracic Surgery, 88 ... Estimation of heart weight by post-mortem cardiac magnetic resonance imaging. Journal of Forensic Radiology and Imaging, 1(1): ...
  http://www.zora.uzh.ch/view/authorsnew/Alkadhi=3AH=3A=3A.html
*  Protocols and Video Articles Authored by Zulma Gazit
Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts ... To date, there are no adequate molecular imaging techniques, which quantitatively monitor gene expression in vivo in skeletal ... The purpose of this study was to determine whether quantitative CEST (qCEST) MRI can be used to detect pH changes in IVDs in ... We report that CXCR4 overexpression on human CD34+ progenitors using a lentiviral gene transfer technique helped navigate these ...
  https://www.jove.com/author/Zulma_Gazit
*  Protocols and Video Articles Authored by Zulma Gazit (Translated to Turkish)
To date, there are no adequate molecular imaging techniques, which quantitatively monitor gene expression in vivo in skeletal ... The purpose of this study was to determine whether quantitative CEST (qCEST) MRI can be used to detect pH changes in IVDs in ... We report that CXCR4 overexpression on human CD34+ progenitors using a lentiviral gene transfer technique helped navigate these ... ranging from neurodegenerative diseases to cardiac insufficiency. The use of mesenchymal stem cells (MSCs) in spine surgery is ...
  https://www.jove.com/author/Zulma_Gazit?language=Turkish
*  ZOBRAZOVACÍ METODY V LÉKAŘSTVÍ Imaging Methods in Medicine - PDF
ZOBRAZOVACÍ METODY V LÉKAŘSTVÍ Imaging Methods in Medicine MUDr. Tomáš Belšan, CSc. Radiodiagnostické oddělení ÚVN Praha U ... Microscopy Research and Technique (6): HAGEN GM, LIDKE KA, RIEGER B, LIDKE DS, CAARLS W, ARNDT-JOVIN DJ, JOVIN TM. Dynamics of ... Hlavní publikace vážící se k tématu: PELBERG, R., MAZUR, W.: Cardiac CT Angiography Manual, Springer Verlag 2007, 144 p., ISBN ... časné fáze několika signálních cest s finálním efektem v buněčném jádře a cytoskeletu. Chemická struktura proteinu i kódující ...
  http://docplayer.cz/1958128-Zobrazovaci-metody-v-lekarstvi-imaging-methods-in-medicine.html
*  One-Hour Procedure to Isolate Primary Cardiomyocytes from Neonatal Mouse and Rat Hearts | Thermo Fisher Scientific
To demonstrate that our primary cardiomyocyte cultures can serve as good physiological model systems, we looked at the activation of two cell signaling pathways. First, we determined whether we could induce cell death in cultured primary cardiomyocytes by serum deprivation and to assess whether insulin-like growth factor I (IGF-I) might suppress the cell death through activation of a serine/threonine protein kinase Akt [4]. In Day 5 cardiomyocyte cultures, cells were incubated with serum-free medium in the presence or absence of IGF-I for 24 hours, and cell viability was determined by PI staining. The surviving cell number is expressed relative to the untreated cell culture. In the absence of IGF-I, cardiomyocyte viability decreased by 25% after 24 hours of serum withdrawal (Figure 6A). However, cardiomyocyte viability was maintained in the presence of IGF-I. IGF-I-mediated cardiomyocyte protection was abrogated by treatment with 10µM Triciribine, an Akt inhibitor V [4], suggesting that the ...
  http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/one-hour-procedure-isolate-primary-cardiomyocytes-neonatal-mouse-rat-hearts.html
*  Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human...
In vitro human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) can differentiate into functional cardiomyocytes (CMs). Protocols for cardiac differentiation of hESCs and
  https://link.springer.com/article/10.1007%2Fs00380-013-0436-9
*  Spark-Induced Sparks As a Mechanism of Intracellular Calcium Alternans in Cardiac Myocytes | Circulation Research
Intracellular calcium (Ca) cycling plays a central role in cardiac excitation-contraction coupling.1,2 Ca alternans, a beat-to-beat alternation in intracellular Ca transient amplitude, is an important factor promoting T-wave alternans and pulsus alternans, markers conferring an increased risk of sudden cardiac death.3 Although Ca alternans has been widely studied in cardiac myocytes,4-13 the underlying mechanism remains controversial. Eisner et al4 were the first to propose that Ca alternans could be explained by a steep nonlinear dependence of sarcoplasmic reticulum (SR) Ca release on the diastolic SR Ca load immediately preceding the release (a steep fractional release-load relationship). This mechanism requires that diastolic SR Ca load alternate concomitantly with SR Ca release. Subsequent experimental6,7,9 and theoretical9,14,15 studies have provided evidence supporting this mechanism. However, later ...
  http://circres.ahajournals.org/content/106/10/1582
*  Trophic Effect of Human Pericardial Fluid on Adult Cardiac Myocytes | Circulation Research
The present study demonstrates that (1) human PF has a greater trophic effect than human serum on cultured rat adult cardiac myocytes, which is likely due to a high concentration of FGF2, and (2) both serum and PF from patients with cardiac hypertrophy have additional effects on cardiac myocytes that are related to the increase in LV mass and that suggest the presence of a circulating growth factor(s) involved in the process of hypertrophy.. One of the major findings of the present study is that PF has a hypertrophic effect on cardiac myocytes, as indicated by (1) the increase of MyHC mRNA level, (2) increased rate of protein synthesis, and (3) increase in total protein content. This trophic effect is not associated with a shift in myosin isoforms, since both α- and β-MyHC mRNAs increase. Furthermore, PF does not seem to enhance apoptosis, since the percentage of cells dying ...
  http://circres.ahajournals.org/content/81/5/679
*  Transmembrane protein 88: a Wnt regulatory protein that specifies cardiomyocyte development | Development
Genetic regulation of the cell fate transition from lateral plate mesoderm to the specification of cardiomyocytes requires suppression of Wnt/β-catenin signaling, but the mechanism for this is not well understood. By analyzing gene expression and chromatin dynamics during directed differentiation of human embryonic stem cells (hESCs), we identified a suppressor of Wnt/β-catenin signaling, transmembrane protein 88 (TMEM88), as a potential regulator of cardiovascular progenitor cell (CVP) specification. During the transition from mesoderm to the CVP, TMEM88 has a chromatin signature of genes that mediate cell fate decisions, and its expression is highly upregulated in advance of key cardiac transcription factors in vitro and in vivo. In early zebrafish embryos, tmem88a is expressed broadly in the lateral plate mesoderm, including the bilateral heart fields. Short hairpin RNA targeting of TMEM88 during hESC cardiac differentiation increases ...
  http://dev.biologists.org/content/early/2013/08/07/dev.094789
*  Letter by Sluijter et al Regarding Article, "Human Cardiac Stem Cell Differentiation Is Regulated by a Mircrine Mechanism" |...
To the Editor:. We read with interest the article by Hosoda et al,1 "Human Cardiac Stem Cell Differentiation Is Regulated by a Mircrine Mechanism." We are pleased to read that Hosoda et al were able to confirm our observations that miR-499 is involved in the differentiation of human cardiac-derived progenitor cells.2. Hosoda and colleagues acknowledged our previous observations in human cardiomyocyte progenitor cells (hCMPCs), but claimed that these cells were erroneously considered a class of cardiac progenitor cells. The authors suggest that our results demonstrated that miR-1 and 499 favor a more mature phenotype in fetal-neonatal cardiomyocytes. Although controversy still exists about different populations of progenitor and stem cells in the heart, we used an exceptionally profound characterized and well-documented cell population,3-5 both present in the fetal and adult human myocardium. hCMPCs do ...
  http://circ.ahajournals.org/content/124/17/e456
*  Establishment of β-Adrenergic Modulation of L-Type Ca2+ Current in the Early Stages of Cardiomyocyte Development | Circulation...
The lack of β-adrenergic modulation in VEDS cardiomyocytes suggests that one or more components of the signaling cascade were missing or functionally inactive. To identify these components, we assayed the functional activity of different elements in the β-adrenergic enzymatic cascade. A key finding was that ICaL was strongly stimulated by cell dialysis with the catalytic subunit of PKA in both VEDS and LDS cardiomyocytes. In contrast to a previous report6 in which fast current rundown was observed using Ba2+ as a charge carrier, we could accurately measure the stimulating effect of the catalytic subunit of PKA on ICaL in VEDS cardiomyocytes. The catalytic subunit of PKA produced a maximal ICaL stimulation. Importantly, ICaL stimulation by the catalytic subunit of PKA was found to be similar (≈3 times) in VEDS, LDS, and ventricular cardiomyocytes isolated from the adult mouse heart. These data provide strong evidence for similar structural properties of the Ca2+ channel or a closely related ...
  http://circres.ahajournals.org/content/84/2/136
*  Alpha-Catenins Control Cardiomyocyte Proliferation by Regulating Yap ActivityNovelty and Significance | Circulation Research
The transcriptional coactivator Yap, a downstream effector of the Hippo signaling pathway, was shown recently to be necessary and sufficient for cardiomyocyte proliferation.19,20 Moreover, cardiac-specific expression of activated Yap (eg, αMHC-YapS112A) that cannot be phosphorylated by inhibitory upstream kinases in the Hippo pathway enhances cardiac regeneration post MI.22,23 However, the upstream regulators that control Yap activity in the heart remain largely unknown. In the present study, we demonstrate that depletion of αE- and αT-catenin in the postnatal heart leads to nuclear accumulation of Yap and induction of cardiomyocyte proliferation. Importantly, deletion of both α-catenin genes in adult heart before injury (IN-DKO model) led to improved cardiac function compared with control littermates. A 2-fold increase in nuclear Yap in cardiomyocytes of the injured IN-DKO relative to WT hearts suggests ...
  http://circres.ahajournals.org/content/116/1/70
*  Migration of cardiomyocytes is essential for heart regeneration in zebrafish | Development
Adult zebrafish possess a significant ability to regenerate injured heart tissue through proliferation of pre-existing cardiomyocytes, which contrasts with the inability of mammals to do so after the immediate postnatal period. Zebrafish therefore provide a model system in which to study how an injured heart can be repaired. However, it remains unknown what important processes cardiomyocytes are involved in other than partial de-differentiation and proliferation. Here we show that migration of cardiomyocytes to the injury site is essential for heart regeneration. Ventricular amputation induced expression of cxcl12a and cxcr4b, genes encoding a chemokine ligand and its receptor. We found that cxcl12a was expressed in the epicardial tissue and that Cxcr4 was expressed in cardiomyocytes. We show that pharmacological blocking of Cxcr4 function as well as genetic loss of cxcr4b function causes failure to regenerate the heart after ventricular resection. Cardiomyocyte proliferation was not affected ...
  http://dev.biologists.org/content/early/2012/10/03/dev.079756
*  Angiotensin II Induces Cardiac Phenotypic Modulation and Remodeling In Vivo in Rats | Hypertension
Accumulating evidence supports the notion that the renin-angiotensin system exists not only in the blood circulation but also in cardiac tissues.34 35 Recently, it has been reported that Ang II induces hypertrophy of cultured cardiac myocytes from neonatal rats9 and embryonic chick8 and also causes the proliferation of cultured neonatal rat cardiac fibroblasts.9 Furthermore, very recently, using an in vitro model of load (stretch)-induced cardiac hypertrophy, Sadoshima et al10 have obtained evidence showing that mechanical stretch leads to release of Ang II from neonatal rat ventricular myocytes and the released Ang II acts as an initial mediator of the stretch-induced hypertrophic response. All these findings, obtained by in vitro studies,8 9 10 support the notion that autocrine release of Ang II from cardiac myocytes is involved in load ...
  http://hyper.ahajournals.org/content/25/6/1252?ijkey=fc355b0b00cfa7b0f95802bc159564a09f7bfc6f&keytype2=tf_ipsecsha
*  NIOSHTIC-2 Publications Search - 00219801 - H2O2-induced oxidative injury in rat cardiac myocytes is not potentiated by 1,1,1...
The ability of the halocarbons 1,1,1-trichloroethane (71556), carbon- tetrachloride (56235) and halothane (151677) to enhance hydrogen- peroxide (7722841) (H2O2) induced oxidative injury in isolated rat neonatal cardiac myocytes was investigated. Myocytes were separated from cardiac ventricular cells isolated from 2 to 4 day old Sprague- Dawley-rats. Cells were exposed in culture to 20 to 200 micr
  https://www.cdc.gov/niosh/nioshtic-2/00219801.html
*  Cell Logic for Dual Coupling of a Single Class of Receptors to Gs and Gi Proteins | Circulation Research
Programmed cell death or apoptosis has been recently implicated as a consequence of cardiac ischemic/reperfusion injury, contributing to the transition from cardiac hypertrophy to decompensatory heart failure.15 16 17 In vivo and in vitro studies indicate that β1AR and β2AR exhibit distinctly different, even opposing, effects on cardiac myocyte apoptosis. In cultured adult rat cardiac myocytes, stimulation of β1AR, but not β2AR, induces myocyte apoptosis.18 Using a β1β2AR double knockout mouse model in conjunction with adenoviral gene transfer, we have created pure βAR subtype experimental settings and found that β1AR stimulation markedly induces myocyte apoptosis. In contrast, β2AR stimulation activates concurrent apoptotic and survival signals mediated by Gi and Gs, respectively, with the Gi-mediated survival effect predominant (Zhu W-Z, Zheng M, Kolbilka BK, Xiao R-P, ...
  http://circres.ahajournals.org/content/87/8/635
*  Abstract 191: Phosphodiesterase 9a Modulates Cardiomyocyte Natriuretic Peptide-Stimulated cGMP Pool and Cardiac Hypertrophy |...
Cardiac hypertrophy in response to pathologic stress is a major contributor to heart disease. Many studies have revealed that compartmentalized cyclic guanosine monophosphate (cGMP) regulation by phosphodiesterases (PDEs) can potently modify acute and chronic cardiac stress responses. PDE5a normally regulates cGMP generated from nitric oxide (NO)-stimulated soluble guanylate cyclase (sGC) but not natriuretic peptide (NP)-stimulated cGMP. Its inhibition blunts maladaptive hypertrophy and remodeling. Regulators of NP-cGMP remain uncertain. Here we reveal that PDE9a, a highly cGMP-specific PDE (×100 affinity vs PDE5a) that is expressed predominantly in the brain modulates myocyte cGMP as well, targeting NP-stimulated pools. PDE9a gene expression is observed in rat neonatal cardiac myocytes (RNCM) and adult mouse myocytes. Protein expression was identified in myocytes by immunohistochemistry, using gene ...
  http://circres.ahajournals.org/content/113/Suppl_1/A191
*  Stable clones of HL-1 myocyte cell lines - Quicktech
Cardiac structure and functionare commonly studied using primary culture of neonatal and adult cardiac myocyte. However, their inability to divide and retain their differentiated phenotype in culture limits their use.. Established from a mouse atrial myocyte tumour, HL-1 cells share similar characteristics with primary cultures of cardiac myocytes. They have the ability to proliferate while keeping a differentiated cardiomyocyte phenotype in culture (this allows the use of specific molecular tools as RNA interference). However, there are concerns about their genetic stability and some studies have shown the cells to contain a functionally heterogeneous population.. The team from Imperial College isolated homogeneous and stable clones of HL-1 cell lines - thereby excluding any differences due to cellular heterogeneity of the original cell line - that display phenotypic characteristics consistent with ...
  http://quicktech.imperialinnovations.co.uk/i/Cell_Lines/HL-1_clones.html
*  Plus it
Cell proliferation is regulated by the balance between cyclin-dependent kinases (CDKs) and CDK inhibitors such as p27. In neonatal cardiomyocytes, p27 is a key inhibitor of cell proliferation (6) and cyclin D1 is important for cell cycle progression (36). To delineate the pathway through which TIMP-3 inhibits neonatal cardiomyocyte proliferation, the effect of TIMP-3 on cyclin D1 and p27 expression was investigated. Our data showed that cyclin D1 was increased in TIMP-3−/− cardiomyocytes and decreased in rTIMP-3-treated cells as compared with WT. Consistent with these results, p27 expression was decreased in the TIMP-3−/− cardiomyocytes and neonatal hearts as compared with WT. This decrease in p27 expression resulted in an increase in cardiomyocyte proliferation both in vitro and in vivo. Furthermore, treatment of cardiomyocytes with rTIMP-3 resulted in a significant increase in p27 expression, which led to a significant decrease in cardiomyocyte proliferation. Our data suggest that ...
  http://ajpcell.physiology.org/content/296/4/C735
*  Activation of Distinct cAMP-Dependent and cGMP-Dependent Pathways by Nitric Oxide in Cardiac Myocytes | Circulation Research
The findings in the present study indicate that the exogenous administration of the NO donor SNAP can induce opposing effects on myocyte contraction, in agreement with recent reports.8 9 Additional experiments using NOC5, an NO donor chemically distinct from SNAP, confirm these observations. Although the direction of the myocardial response clearly depended on the concentration of the NO donor used and on the level of intracellular cGMP reached, the principal novel finding of the present study is that a previously unrecognized GC/cGMP-independent mechanism involving the activation of AC is also critical in mediating this response.. In rat cardiac myocytes, high concentrations of SNAP (producing ≈14.7 μmol/L NO) induced large increases in cGMP and reduced myocyte contraction, whereas low concentrations of SNAP (producing in the range of ≈0.1 to 0.5 μmol/L NO) induced only moderate increases in cGMP and enhanced myocyte contraction. Furthermore, these contractile effects were ...
  http://circres.ahajournals.org/content/84/9/1020
*  Plus it
It is believed that in embryonic cardiomyocytes the myocardial contraction is predominantly dependent on transsarcolemmal Ca2+ influx (26, 36). Therefore, it is expected that in embryonic cardiomyocytes, NCX might be a prominent Ca2+ remover and even more important than SERCA. However, it has been recently shown that in murine embryonic cardiomyocytes 8.5-9.5 days old, the majority of Ca2+ removal (∼74%) is accomplished by SERCA (32). The studies on mESCMs showed that the SERCA is the major Ca2+ remover in 17-day-old mESCMs (16). Here, we provided evidence showing that the contribution of SERCA to Ca2+ removal is upregulated with the time of mESCMs differentiation, accompanied with a downregulation of NCX to Ca2+ removal, a similar pattern as reported by Kapur and Banach (16). The functional changes with differentiation also coincide with the gene expression pattern of SERCA and NCX during embryonic development (21, 32) or mESCMs differentiation (12). Moreover, our data demonstrated that ...
  http://ajpcell.physiology.org/content/297/3/C732
*  Ca2+-regulatory proteins in cardiomyocytes from the right ventricle in children with congenital heart disease | Journal of...
Previous studies have reported that immature cardiomyocytes have characteristics of Ca2+-regulatory proteins which are different from that of mature cardiomyocytes [22-25]. During normal cardiac development in rabbits and rats, NCX expression is maximal near the time of birth and then declines postnatally [22, 25]. Conversely, SERCA2a expression levels increased during this period [23-25]. The characteristics of Ca2+-regulatory proteins in immature cardiomyocytes during hypertrophy with or without hypoxia has not been studied extensively, however the results of research will be beneficial for choosing an optimal time of therapy and interpreting the development process of cardiomyocytes in children with CHD.. To our knowledge, this study could be the first effort specifically dedicated to the evaluation of the gene and protein expression of Ca2+-regulatory proteins on human immature cardiomyocytes from RV. There were three major findings in this study. We found that SERCA2a, ...
  https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-10-67
*  Protocols and Video Articles Authored by Lijun Liu
Lijun Liu is the author of these articles in the Journal of Visualized Experiments: 'Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy', 'A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma'
  https://www.jove.com/author/Lijun_Liu
*  The Molecular Basis for Distinct β-Adrenergic Receptor Subtype Actions in Cardiomyocytes | Circulation Research
Similar to the scenario in neonatal rat cardiomyocytes, β1-AR stimulation with isoproterenol induces a robust increase in cAMP accumulation that is associated with an increase in the amplitude and an acceleration of the kinetics of both the calcium transient and the twitch in adult rat ventricular myocytes. In contrast, only rather high concentrations of the β2-AR agonist zinterol (10-5 mol/L) modulate contractile function in adult rat ventricular myocytes (Figure 2⇑). Parenthetically, 2 laboratories have presented data to support the theoretical argument that 10-5 mol/L zinterol (in the absence of a β1-AR blocker) acts as a mixed β1-/β2-AR agonist; β2-AR selectivity is achieved only if a β1-AR blocker (such as CGP 20712A) is included in the assay.11 73 In contrast, Xiao and Lakatta74 maintain that the effects of high concentrations of zinterol (even without a β1-AR blocker) are attributable entirely to the minor population of β2-ARs in the preparation, and they present results to ...
  http://circres.ahajournals.org/content/85/11/1101.long
*  Some things you don't have any control over: GERN - Cool company doing cool stuff.(Not A Stock Recommendation, do your own...
In the studies reported in the American Journal of Pathology, Dr. Charles Murry and his colleagues at the University of Washington, in collaboration with scientists at Geron Corporation, produced cardiomyocytes from hESCs and injected the cells into the left ventricular wall of normal healthy rats. The transplant was examined one and four weeks after injection and human cells were detected in the rat myocardium at both timepoints. At the four week timepoint, the grafted human cardiomyocytes were identified using characteristic markers including sarcomeric actin, alpha and beta-myosin heavy chain, and myosin light chain 2v. Approximately 10% of the cardiomyocytes at the four week timepoint retained proliferative capacity, thereby potentially enhancing engraftment. Both host and graft derived angiogenesis (new blood vessel formation) was observed, critical to sustaining the viability of the graft. No evidence of tumor formation was seen ...
  http://evatt.blogspot.com/2005/09/gern-cool-company-doing-cool-stuffnot.html
*  Downregulation of cardiac myocyte Na+-K+-ATPase by adenovirus-mediated expression of an α-subunit fragment | Heart and...
This study was initiated with the original aim of assessing the consequences of the expression of the functional α2-subunit of the rat Na+-K+-ATPase in the neonatal rat cardiac myocytes lacking this isoform. It soon became evident, however, that only a truncated α2-subunit not likely to be functional was overexpressed in these cells. Because enzyme and receptor fragments may often act like inactive mutant variants and cause dominant negative inhibition (2, 10, 12, 23, 27, 36) we attempted to determine whether the expression of the α2-fragment impaired the function of endogenous Na+-K+-ATPase in the neonatal myocytes. Our findings clearly show that the ion transport function of Na+-K+-ATPase is indeed inhibited concomitant with the expression of the truncated α2-isoform and that this is accompanied by a significant reduction of the α1-protein content of the neonatal myocyte. Because the induced reduction of the α3-protein content is small, if any, and because it is ...
  http://ajpheart.physiology.org/content/280/3/H1415.long
*  Gene Expression Literature Detail
J:169706 Abdul-Ghani M, Dufort D, Stiles R, De Repentigny Y, Kothary R, Megeney LA, Wnt11 promotes cardiomyocyte development by caspase-mediated suppression of canonical Wnt signals. Mol Cell Biol. 2011 Jan;31(1):163-78 ...
  http://www.informatics.jax.org/gxdlit/key/121835
*  Research: Statins cause heart disease
New research shows that statins, drugs which lower cholesterol, cause heart disease. These drugs cause calcified plaques to form in coronary arteries in humans, thus causing or worsening heart disease.
  http://www.digitaljournal.com/article/334943
*  Role of Meis1 in Regulation of Cardiomyocyte Proliferation - Hesham Sadek
Heart failure is a costly and deadly disease, affecting over 23 million patients worldwide, 5.8 million patients in America, half of which die within 5 years of...
  http://grantome.com/grant/NIH/R01-HL115275-02