Immunofluorescence - Wikipedia
Carbohydrate sequence specificity of the mouse monoclonal antibodies that recognize crossreacting antigens on LOS and human ... Nonspecific fluorescence involves the loss of a probes specificity due to fluorophore, from improper fixation, or from a dried ... This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets ... Additionally, the binding of the fluorophore to the antibody itself cannot interfere with the immunological specificity of the ...
Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013) | Abcam
Karina Persson
Lactobacillus johnsonii ameliorates intestinal, extra-intestinal and systemic pro-inflammatory immune responses following...
Lactobacillus johnsonii La1 shares carbohydrate-binding specificities with several enteropathogenic bacteria. Glycobiology 10, ... Genome Sequence of Lactobacillus johnsonii Strain W1, Isolated from Mice. Genome Announc 4, doi:10.1128/genomeA.00561-16 (2016 ... johnsonii by molecular analysis of the complete 16S rRNA gene sequence.. For bacterial in vivo competition experiments, C. ... Bacterial species were identified according to their typical morphological appearances and sequencing of the 16S rRNA genes. ...
ID ADK HUMAN STANDARD; PRT; 362 AA
CC -!- SIMILARITY: Belongs to the carbohydrate kinase pfkB family. CC -!- CAUTION: Ref.2 sequence differs from that shown due ... Sequence=VSP_004668; CC -!- TISSUE SPECIFICITY: Widely expressed. Highest level in placenta, CC liver, muscle and kidney. ... 1; AA SEQUENCE). FT CONFLICT 289 289 I -> N (IN REF. 1; AA SEQUENCE). FT CONFLICT 307 307 K -> R (in Ref. 2). FT TURN 23 24 FT ... OX NCBI_TaxID=9606; RN [1] RP SEQUENCE FROM N.A., AND SEQUENCE OF 94-133; 175-200 AND 272-289. RC TISSUE=Liver; RX MEDLINE= ...
Systematic Screening of Synthetic Gene-Encoded Enzymes for Synthesis of Modified Glycosides - DOE Joint Genome Institute
... diverse libraries of synthetic genes from a sequence-based family allows rapid evaluation of the substrate specificity encoded ... For carbohydrate-active enzymes such as glycosidases, this opens up approaches to search for enzymes that are capable of ... Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the ... the most active such enzymes were further characterized through detailed kinetic analysis and plate-based aglycone specificity ...
Sally Prime CV
... vivo glycoprotein synthesis with the modelling of rules for exo-glycosidase specificity to deduce the sequence of carbohydrates ... Design and implementation of carbohydrate sequencing analysis software application (EVE) which coupled the biological modelling ... R Parekh, S Prime, Sequencing of oligosaccharides US Patent number 5427744. (1995). G Guile, P Rudd, D Wing, S Prime, R Dwek ... R Parekh, S Prime, Sequencing Loop Apparatus US Patent number 5667984. (1997). R Parekh, S Prime, R Townsend, N Wedd, A ...
Mouse anti CD65s - Nordic MUbio
Specificity Antibody VIM2 reacts with a carbohydrate structure expressed by myeloid cells. The epitope recognized was shown by ... to involve a defined sialofucooligosaccharide sequence. Similar results were obtained by Kniep et al.. Together with other ... sialylated and fucosylated polylactosamines the carbohydrate structure recognized by VIM2 may play a critical role on the ...
Michael Breimer | Göteborgs universitet
Carbohydrate xenoantigens and xenoantibody epitope-specificity, the removal of xenoantibodies by plasmapheresis, and antibody ... He has published more than 150 scientific articles in the fields of carbohydrate biochemistry, glycobiology, allo- and xeno- ... H and related carbohydrate antigens. After his PhD examination, his biochemical studies relating to ABO-incompatible organ ... sequence. Jonas Ångström, Michael Breimer, K E Falk, Gunnar C. Hansson, K A Karlsson, H Leffler, I Pascher Archives of ...
Exploring molecular determinants of polysaccharide lyase family 6-1 enzyme activity. - The Division of Structural Biology
... various types of alginate in a very similar manner but with a series of original adaptations bringing them their specificities ... which all show different substrate specificities and mode of action (endo-/exolyase). Thorough exploration of the structures of ... Humans, Polysaccharide-Lyases, Crystallography, X-Ray, Amino Acid Sequence, Carbohydrate Conformation, Substrate Specificity, ... which all show different substrate specificities and mode of action (endo-/exolyase). Thorough exploration of the structures of ...
Author Report for: Biely P
... there is a need to expand the sequence database in order to strengthen the link between AcXE gene sequence and specificity. ... Its substrate specificity was examined on a number of substrates of other carbohydrate esterases such as acetylxylan esterase, ... Title: Substrate specificity of acetylxylan esterase from Schizophyllum commune: mode of action on acetylated carbohydrates. ... Comparison of the amino acid sequences of identified and putative glucuronoyl esterases to other types of carbohydrate ...
Pesquisa | Portal Regional da BVS
... ß-galactoside-binding specificity and a consensus sequence in their carbohydrate-recognition domain. Galectin-1 (Gal-1) is ... The consensus sequences homology search was performed using BLASTn analysis and showed a 100% of homology with the C. pecorum ... The 16S rRNA subunit and ompA gene amplicons were purified and the nucleotide sequence was determined in both directions. ... The population structure analyses using ompA gene demonstrated 15 genetic populations or clusters of 198 sequences from GenBank ...
Catechol oxidase. Medical search
5. Digestion of 50% of the carbohydrate content leads to complete loss of serological specificity (serological reaction and ... Amino-terminal sequencing allowed us to design oligonucleotide probes for isolation of cDNA clones derived from the salivary ... The full sequence of the cDNA demonstrated homology between A. albimanus salivary peroxidase and several members of the ... The substrate specificity of catechol oxidase from Lycopus europaeus towards phenols is examined. The enzyme catalyzes the ...
Anti-C3b / iC3b antibody [7C12] (ab231078) | Abcam
Tissue specificity. Plasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma ... After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. ... Sequence similarities. Contains 1 anaphylatoxin-like domain.. Contains 1 NTR domain.. * Post-translational. modifications. C3b ...
Team defines first-ever sequence of biologically important carbohydrate - Research & Development World
... there is no known mechanism for controlling that type of specificity." ... Team defines first-ever sequence of biologically important carbohydrate. By R&D Editors , October 18, 2011. ... Previous studies performed over the past five years by the researchers that identified common sequences within the carbohydrate ... Past attempts to sequence proteoglycans have relied on the so-called "bottom up" approach in which scientists use enzymes to ...
Home | Max Planck Institute of Colloids and Interfaces
Portable sulphotransferase domain determines sequence specificity of heparan sulphate 3-O-sulphotransferases. Biochemical ... Haase, W.-C.; Seeberger, P. H.: Recent progress in polymer-supported synthesis of oligosaccharides and carbohydrate libraries. ... Seeberger, P. H.; Haase, W.-C.: Solid-phase oligosaccharide synthesis and combinatorial carbohydrate libraries. Chemical ...
dbCAN-seq: a database of CAZyme sequence and annotation
Lectins are important adhesin proteins, which bind carbohydrate structures on host cell surface. The carbohydrate specificity ... You are here: Home , Sequence: MGYG000000631_01441. Basic Information , Genomic context , Full Sequence , Enzyme annotations , ... Most members of this family appear to have signal sequences. Members from the dental pathogen Porphyromonas gingivalis have ... bacteria tissue tropism by mediating specific attachment to unique host sites expressing the corresponding carbohydrate ...
DeCS
Contiguous amino acid sequences within a carbohydrate-active enzyme with discrete folds having carbohydrate binding activity. ... They are classified into 43 different families based on AMINO ACID SEQUENCE, binding specificity, and structure. ... Carbohydrate Binding Modules Entry term(s). Binding Module, Carbohydrate Carbohydrate Binding Module Module, Carbohydrate ... Contiguous amino acid sequences within a carbohydrate-active enzyme with discrete folds having carbohydrate binding activity. ...
Pathogen Surveillance Through Monitoring of Sewer Systems - ScienceOpen
... specificity), detection and differentiation of the signal from interfering sequences (sensitivity), and automation of all ... These methods use latex beads, carbohydrates, anion exchange resins, or similar substances as part of sample collection and ... However, today this can be accomplished by the use of molecular methods and sequence analysis. Sequences amplified directly ... Vaccine strains have unique sequences from wild type strains of pathogens allowing easy differentiation. In addition, sequence ...
6gry - Proteopedia, life in 3D
2009 , Knudsen, R Meland , Larsbrink, J , Leggio, L Lo , Mazurkewich, S , Poulsen, J C.Navarro , Carbohydrate esterase , ... 6gry is a 1 chain structure with sequence from "candidatus_solibacter_usitatus"_ward_et_al._2009 "candidatus solibacter ... Similarities and differences regarding substrate specificity between the investigated GEs were observed and putatively linked ... Results: Ten CE15 enzymes from three bacterial species, sharing as little as 20% sequence identity, were characterized on a ...
Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous...
The resulting sequences were submitted to functional annotation against non-redundant NCBI database and Database of Anuran ... Total RNA from the toad skin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end ... The fractions were partially sequenced by Edmans degradation. We were able to identify several classes of antimicrobial ... peptides, such as buforins, peroniins and brevinins, as well as PLA2, lectins and galectins, combining protein sequencing and ...
11beta-hydroxysteroid dehydrogenase type 1: a tissue-specific regulator of glucocorticoid response. - Radcliffe Department of...
Organ Specificity | Harvard Catalyst Profiles | Harvard Catalyst
Transcriptome variation in human tissues revealed by long-read sequencing. Nature. 2022 08; 608(7922):353-359. ... Protein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase. Science. 2023 03 10; ... "Organ Specificity" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Organ Specificity" by people in Harvard Catalyst Profiles by ...
Protein Technologies Pharmaceutical glossaries & Taxonomies
Glycoarrays--tools for determining protein-carbohydrate interactions and glycoenzyme specificity. Laurent N1, Voglmeir J, ... peptide sequencing: How is this different from protein sequencing (except that peptides are shorter than proteins)? phage: A ... Related terms: antibody arrays, carbohydrate chips, carbohydrate arrays, protein arrays, protein microarrays; Narrower terms: ... glycoarrays: Carbohydrate arrays (glycoarrays) have recently emerged as a high-throughput tool for studying carbohydrate- ...
Saccharophagus degradans: Difference between revisions - microbewiki
There are also around 112 ORFs that contain catalytic and/or carbohydrate-binding modules (CBM) with specificity for plant- ... of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. Include a ... Both of these showed the presence of multiple 6 carbohydrate binding modules (CBM6) with Aga16B having two homologs of CBM6 and ... Both of these showed the presence of multiple 6 carbohydrate binding modules (CBM6) with Aga16B having two homologs of CBM6 and ...
Highly Pathogenic Influenza A(H5Nx) Viruses with Altered H5 Receptor-Binding Specificity - Volume 23, Number 2-February 2017 -...
Gu M, Huang J, Chen Y, Chen J, Wang X, Liu X, et al. Genome sequence of a natural reassortant H5N2 avian influenza virus from ... Complete structure of two carbohydrate units of human serotransferrin. FEBS Lett. 1975;50:296-9. DOIPubMedGoogle Scholar ... HA binding displays a clear receptor fine specificity, but substrate fine specificity of NAs has not been extensively ... Wickramasinghe IN, de Vries RP, Eggert AM, Wandee N, de Haan CA, Gröne A, et al. Host tissue and glycan binding specificities ...
The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor |...
We identified a lipA gene in the genome sequence of Acremonium alcalophilum, encoding a protein with a predicted lipase domain ... Unusually, the predicted lipase displays ≈ 30% amino acid sequence identity to both feruloyl esterase and lipase of Aspergillus ... The genome sequence of Acremonium alcalophilum has revealed a large number of genes encoding biomass-degrading enzymes. Among ... including hydroxycinnamoyl and carbohydrate esters. Biochemical characterization of LipA showed a broad specificity towards p ...
Characterization of the aldo-keto reductase 1C gene cluster on pig chromosome 10: possible associations with reproductive...
Comparison of sequence data with the porcine BAC fingerprint map show that the cluster of genes resides in a 300 kb region. ... The nucleotide sequence data reported have been submitted to GenBank and assigned accession numbers [GenBank:DQ474064-DQ474068 ... Screening the porcine CHORI-242 BAC library with a full-length AKR1C4 cDNA identified 7 positive clones and sample sequencing ... These duplicated genes maintain a high degree of sequence similarity, but differ greatly in their substrate specificity and ...
Recombinant Galectin-1 and Its Genetic Delivery Suppress Collagen-Induced Arthritis via T Cell Apoptosis | Journal of...
... a 29-kD member of this protein family with similar carbohydrate specificity. GAL-1 has been shown to induce T cell apoptosis 13 ... 3 and share significant sequence similarities in the carbohydrate recognition domain 1,4. The precise functions of individual ... Specificity was assayed by adding TDG (100 mM) to the culture. Cells were cocultured for 24 h and IL-2 was measured in the ... Specificity was assayed by adding TDG (100 mM) to the culture. The anti-GAL-1 Ab (1:100) had no neutralizing activity (data not ...
Glycosylation in mAb Therapeutic Products: Analytical Characterization and Impact of Process
Glycosylation refers to a sequence of events that take place in the Golgi body and the endoplasmic reticulum of the cells of ... Anion Exchange and Highâ Performance Anion Exchange: Because carbohydrates are weak acids (pKa , 11), they can be retained on a ... use of an enzymatic method is limited because of the variable structure of the O-glycans and the narrow substrate specificity ... The second level of information is with regards to the variation in the sequence of the sugars (microheterogeneity). This ...