Capillary electrophoresis method for the simultaneous determination of carbohydrates and proline in honey samples
A capillary electrophoresis method with indirect UV detection for the simultaneous determination of three carbohydrates (fructose, glucose and sucrose) and the amino acid proline in honey samples was developed. This method included the use of a background electrolyte consisting of 10 mM sodium benzoate and 1.5 mM cetyltrimethylammonium bromide, pH 12.4. Under optimal capillary electrophoresis conditions, the separation of the investigated substances was achieved in less than 5 min and single dilution of each sample was employed. The detection limits for fructose, glucose and sucrose were 0.58 g L-1, 0.67 g L-1 and 0.12 g L-1 respectively, and 0.72 mg L-1 for proline. Precision measurements calculated in terms of %RSD in the range of 0.92 to 5.43%, were obtained. The proposed method was applied to honey samples from Argentina and Sweden and enables the determination of the three carbohydrates ...http://kth.diva-portal.org/smash/record.jsf?pid=diva2:1037263
Capillary Electrophoresis Methods for Pharmaceutical Analysis av George Lunn (Innbundet) | Tanum nettbokhandel
Capillary Electrophoresis Methods for Pharmaceutical Analysis (Innbundet) av forfatter George Lunn. Pris kr 5 899. Se flere bøker fra George Lunn.https://www.tanum.no/_capillary-electrophoresis-methods-for-pharmaceutical-analysis-george-lunn-9780471331889
Capillary electrophoresis - Wikipedia
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels. Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar electrokinetic chromatography (MEKC) belong also to this class of methods. In CE methods, analytes migrate through electrolyte solutions under the influence of an electric field. Analytes can be separated according to ionic mobility and/or partitioning into an alternate phase via non-covalent interactions. Additionally, analytes may be concentrated or "focused" by means of gradients in conductivity and ...https://en.wikipedia.org/wiki/Capillary_electrophoresis
Interaction of albumins and heparinoids investigated by affinity capillary electrophoresis and free flow electrophoresis -...
Abstract A fast and precise affinity capillary electrophoresis ACE method has been applied to investigate the interactions between two serum albumins HSA and BSA and heparinoids. Furthermore, different free flow electrophoresis methods were developed to separate the species which appears owing to interaction of albumins with pentosan polysulfate...http://www.separationsnow.com/details/earlyview/10.1002/elps.201700202/Interaction-of-albumins-and-heparinoids-investigated-by-affinity-capillary-elect.html
Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics 1st edition | 9780824709518 | VitalSource
Buy or Rent Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics as an eTextbook and get instant access. With VitalSource, you can save up to 80% compared to print.https://www.vitalsource.com/products/affinity-capillary-electrophoresis-in-reinhard-h-h-neubert-v9780203912133
Capillary Electrophoresis Systems Market Size, Competitive Trends, Outlook and Forecasts : | Medgadget
QY Research Groups states the newly formulated research report on Capillary Electrophoresis Systems Market to their group of offerings. The report based onhttps://www.medgadget.com/2017/05/capillary-electrophoresis-systems-market-size-competitive-trends-outlook-and-forecasts.html
DIGITAL.CSIC: Metabolomics of adherent mammalian cells by capillary electrophoresis-mass spectrometry: HT-29 cells as case study
In this work, the optimization of an effective protocol for cell metabolomics is described with special emphasis in the sample preparation and subsequent analysis of intracellular metabolites from adherent mammalian cells by capillary electrophoresis-mass spectrometry. As case study, colon cancer HT-29 cells, a human cell model to investigate colon cancer, are employed. The feasibility of the whole method for cell metabolomics is demonstrated via a fast and sensitive profiling of the intracellular metabolites HT-29 cells by capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF MS). The suitability of this methodology is further corroborated through the examination of the metabolic changes in the polyamines pathway produced in colon cancer HT-29 cells by difluoromethylornithine (DFMO), a known potent ornithine decarboxylase inhibitor. The selection of the optimum extraction conditions allowed a ...https://digital.csic.es/handle/10261/113704
"Integrated multiplexed capillary electrophoresis system" by Edward S. Yeung and Hongdong Tan
The present invention provides an integrated multiplexed capillary electrophoresis system for the analysis of sample analytes. The system integrates and automates multiple components, such as chromatographic columns and separation capillaries, and further provides a detector for the detection of analytes eluting from the separation capillaries. The system employs multiplexed freeze/thaw valves to manage fluid flow and sample movement. The system is computer controlled and is capable of processing samples through reaction, purification, denaturation, pre-concentration, injection, separation and detection in parallel fashion. Methods employing the system of the invention are also providedhttp://lib.dr.iastate.edu/patents/156/
Microfluidic Capillary Electrophoresis Systems
Automated microfluidics capillary electrophoresis systems provide rapid alternative to slab gel analysis. Size and quantitate DNA, RNA, and Protein for NGS or biotherapeutics applications.http://www.perkinelmer.com/category/microfluidic-capillary-electrophoresis-systems
"A Capillary Electrophoresis Method for Evaluation of Abeta Proteolysis" by Benjamin J. Alper and Walter K. Schmidt
According to the amyloid hypothesis, Aβ peptides are neurotoxic and underlie development and progression of Alzheimer's disease (AD). Multiple Aβ clearance mechanisms, including destruction of the peptides by proteolytic enzymes, are hypothesized to regulate physiological Aβ peptide levels. The insulin-degrading enzyme (IDE) is considered one of the predominant enzymes having Aβ degrading activity. Despite its putative role in protecting against AD, relatively few methods exist for studying IDE activity in vitro. We report the application of capillary electrophoresis (CE) as a novel method for evaluating IDE-mediated Aβ 1-40 proteolysis. This method employs chemically unmodified substrates that are readily obtained from commercial sources. It involves minimal sample preparation, and requires no specialized equipment beyond a CE instrument equipped with a standard fused silica capillary. In the present analysis, we demonstrate that this ...http://digitalcommons.sacredheart.edu/chem_fac/13/
Capillary electrophoresis separation - General Lab Techniques - BioForum
Capillary electrophoresis separation - posted in General Lab Techniques: Hi, our lab has a problem which studies the properties of DNA polymerases, and we are trying to identify an extremely rare mutation (deletion/insertion) that current sequencing technologies cannot detect with accuracy (i.e. the error is higher than the signal were looking for) so i was wondering if we could use separation by capillary electrophoresis. Heres my rationale, capillary electrophoresis is used in sanger seq...http://www.protocol-online.org/forums/topic/33643-capillary-electrophoresis-separation/
Application of capillary electrophoresis mass spectrometry and liquid chromatography multiple-step tandem electrospray mass...
Otitis media caused by nontypeable Haemophilus influenzae (NTHi) is a common and recurrent bacterial infection of childhood. The structural variability and diversity of H. influenzae lipopolysaccharide (LPS) glycoforms are known to play a significant role in the commensal and disease-causing behavior of this pathogen. In this study, we determined LPS glycoform populations from NTHi strain 1003 during the course of experimental otitis media in the chinchilla model of infection by mass spectrometric techniques. Building on an established structural model of the major LPS glycoforms expressed by this NTHi strain in vitro (M. Mansson, W. Hood, J. Li, J. C. Richards, E. R. Moxon, and E. K. Schweda, Eur. J. Biochem. 269:808-818, 2002), minor isomeric glycoform populations were determined by liquid chromatography multiple-step tandem electrospray mass spectrometry (LC-ESI-MSn). Using capillary electrophoresis ESI-MS (CE-ESI-MS), we determined glycoform profiles for ...http://sh.diva-portal.org/smash/record.jsf?pid=diva2:467030
Rapid separation of protein isoforms by capillary zone electrophoresis with new dynamic coatings - Chang - 2005 -...
Many cellular functions are regulated through protein isoforms. Changes in the expression level or regulatory dysfunctions of isoforms often lead to developmental or pathological disorders. Isoforms are traditionally analyzed using techniques such as gel- or capillary-based isoelectric focusing. However, with proper electroosmotic flow (EOF) control, isoforms with small pI differences can also be analyzed using capillary zone electrophoresis (CZE). Here we demonstrate the ability to quickly resolve isoforms of three model proteins (bovine serum albumin, transferrin, α1-antitrypsin) in capillaries coated with novel dynamic coatings. The coatings allow reproducible EOF modulation in the cathodal direction to a level of 10-9 m2V-1s-1. They also appear to inhibit protein adsorption to the capillary wall, making the isoform separations highly reproducible both in peak areas and apparent mobility. Isoforms of ...http://onlinelibrary.wiley.com/doi/10.1002/elps.200410283/abstract
Determination of aristolochic acid by capillary zone electrophoresis - Open Chemistry - Tom 2, Numer 3 (2004) - Biblioteka...
A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200-6000 ng/mL, 95-103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC ...http://yadda.icm.edu.pl/yadda/element/bwmeta1.element.-psjd-doi-10_2478_BF02476197
"The development of capillary electrophoresis assays to study enzyme in" by Sherrisse Kelly Bryant
New methods for studying enzymes and enzyme inhibition using capillary electrophoresis (CE) were developed and applied. Because CE is a separation technique, spectral interference is minimized by separation of substrates, products, inhibitors, and sample matrix components. Two types of CE enzyme assays were explored in this dissertation research. The first assay was based on optically gated vacancy capillary electrophoresis (OGVCE) with laser-induced fluorescence detection (LIF). This approach involves periodic photobleaching of fluorescent substrates and products. The initial goal of the study was to develop an assay for adenosine deaminase (ADA). A fluorescent ADA substrate was synthesized; however, the substrate was unusually photostable and could not be photobleached. In Chapter 2, a comparative study is presented that quantified the photostability of the fluorescent ADA substrate relative to other ...http://digitalcommons.lsu.edu/gradschool_dissertations/3753/
Determination of phenolic acids by capillary zone electrophoresis and HPLC - Open Chemistry - Tom 6, Numer 3 (2008) -...
Selected phenolic acids are determined by capillary zone electrophoresis and HPLC, each using UV detection. The optimised CZE background electrolyte contained 50 mM acetic acid, 95 mM 6-aminocaproic acid, 0.1% polyacrylamide, 1% polyvinylpyrrolidone, and 10% methanol. Twelve phenolic acids (gallic, p-hydroxybenzoic, 3,4-dihydroxybenzoic, vanillic, syringic, o-coumaric, p-coumaric, caffeic, sinapic, ferulic, salicylic and chlorogenic) were separated within 10 minutes. Chromatographic separation of these phenolic acids was carried out on an Eclipse XBD C8 column using a mobile phase gradient (acetonitrile / methanol / water / 0.1% phosphoric acid); all were separated within 25 minutes. Electrophoretic and chromatographic determinations of ferulic and chlorogenic acids were compared on barley, malt, and potato samples. The methods' characteristics were: linearity (1-20 mg ml and 0.2-4 mg ml−1), accuracy (recovery 94 ± 5% and 96 ± 4%), intra-assay repeatability ...http://yadda.icm.edu.pl/yadda/element/bwmeta1.element.-psjd-doi-10_2478_s11532-008-0032-5
FINGERPRINTS OF HUMIC ACIDS BY CAPILLARY ZONE ELECTROPHORESIS
Received: Januanry 19, 1999 - Accepted: July 5, 1999) SUMMARY Humic substances (HS) have a great importance in regard to the quality and productivity of a soil, and in the retention of the metal ions and pollutants by the environment. The characterization of humic compounds provides important information to evaluate the interaction of HS metal ions and with different classes of organic compounds (e.g., herbicides and pesticides). In order to understand HS behavior, it is necessary to separate this complex organic matter into fractions, and determine the structure of each compound in the fractions obtained. Numerous studies applying many different analytical techniques have been used for this purpose. In particular, the application of capillary zone electrophoresis (CZE) for the characterization of HS has evoked an increased interest. The aim of this work is to study the CZE behavior of HA from a different origin. The HA of Argentinean soils and also the HA ...http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0366-16441999000400002&lng=es&nrm=iso&tlng=es
A new labelling reagent for glycans analysis by capillary electrophoresis-mass spectrometry
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.https://www.growkudos.com/publications/10.1039%25252Fc2ay05741g/reader
Intergrating an internal standard in capillary electrophoresis based lab on a chip
Allison Bidulock. The aim of this project is to devise a way to incorporate an internal standard in point-of-care devices based on capillary electrophoresis. Capillary electrophoresis suffers from variations in injected sample amount, applied injection and separation voltages, electroosmotic flow velocity, sample interactions with the wall, etc. etc. These variations are exacerbated when disposable chips are used. An internal standard, added to the sample prior to analysis, can substantially correct for many of these errors. To add such an internal standard is however not simple for a point-of-care device, since it is meant for operation by the patient, who should just perform very simple actions to apply the sample. In a first stage the project has resulted in an experimental analysis of the improvement in chip-to-chip variance on the addition of internal standard. A considerable improvement was observed, ...https://www.utwente.nl/ewi/bios/research/micronanofluidics/the-incorporation-of-an-internal-standard-in-chip/
Non-aqueous capillary electrophoresis with red light emitting diode absorbance detection for the analysis of basic dyes - Open...
Non-aqueous capillary electrophoresis was evaluated for the separation of five hydrophobic basic blue dyes for application in forensic dye analysis. The use of a red light emitting diode as a high intensity, low-noise light source provided sensitive detection of the blue dyes while also allowing the evaluation of solvents that absorb strongly in the UV region. Excellent peak shapes and separation selectivity were obtained in methanol, ethanol, acetonitrile and dimethylsulfoxide, however water, tetrahydrofuran, dimethylformamide and acetone were unsuitable as solvents due to poor peak shapes and a lack of sensitivity, most likely due to adsorption onto the capillary wall. Due to the known compatibility of methanol with capillary electrophoresis-mass spectrometry, this solvent was examined further with the relative acidity/basicity of the electrolyte being optimised with an artificial neural ...https://eprints.utas.edu.au/6584/
Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation
Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum albumin, β-lactoglobulin, horse myoglobin, cytochrome c) has been chosen as a peptide test mixture. After assessment of different modes of capillary electrophoresis as a second dimension to Off-Gel isoelectric focusing, the optimized two-dimensional platforms provide a degree of orthogonality comparable to state-of-the-art multidimensional liquid chromatography systems as well as a practical peak capacity above 700.. ...https://infoscience.epfl.ch/record/114819
CLINICAL AND FORENSIC APPLICATIONS OF CAPILLARY ELECTROPHORESIS (PATHOLOGY AND LABORATORY MEDICINE) by PETERSEN JOHN R:...
Synopsis: John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.. ...https://www.abebooks.com/CLINICAL-FORENSIC-APPLICATIONS-CAPILLARY-ELECTROPHORESIS-PATHOLOGY/20392240760/bd
Capillary Electrophoresis of Biomolecules: Methods and Protocols - Nicola Volpi; Francesca Maccari; | Foyles Bookstore
Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In Capillary Electrophoresis of Biomolecules: Methods and Protocols, expert researchers in the field provide key techniques to investigate CE focusing on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. Along with practical procedures, reviews discussing CE applications related to bio(macro)molecules are also included. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and ...http://www.foyles.co.uk/witem/science-mathematics/capillary-electrophoresis-of,nicola-volpi-francesca-maccari-9781493962488
OSA | Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides
Chaitanya Dongre,1,* Ronald Dekker,1 Hugo J.W.M. Hoekstra,1 Markus Pollnau,1 Rebeca Martinez-Vazquez,2 Roberto Osellame,2 Giulio Cerullo,2 Roberta Ramponi,2 Rob van Weeghel,3 Geert A.J. Besselink,4 and Hans H. van den Vlekkert4 1Integrated Optical MicroSystems, MESA+ Institute for Nanotechnology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands 2Istituto di Fotonica e Nanotecnologie del CNR, Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy 3Zebra Bioscience BV, W. Beversstraat 185, 7543 BK Enschede, The Netherlands 4LioniX BV, PO Box 456, 7500 AH Enschede, The Netherlands *Corresponding author: C.Dongre@ewi.utwente.nl ...https://www.osapublishing.org/ol/abstract.cfm?uri=ol-33-21-2503
Patent US7329333 - Uniform laser excitation and detection in capillary array electrophoresis ... - Google Patents
A capillary electrophoresis system comprises capillaries positioned in parallel to each other forming a plane. The capillaries are configured to allow samples to migrate. A light source is configured to illuminate the capillaries and the samples therein. This causes the samples to emit light. A lens is configured to receive the light emitted by the samples and positioned directly over a first group of the capillaries and obliquely over a second group of the capillaries. The light source is further configured to illuminate the second group of capillaries more than the first group of the capillaries such that amount of light received by the lens from the first group of capillaries is substantially identical to amount of light received from the second group of capillaries when an identical amount of the samples is migrating through the first and second group capillaries.http://www.google.ca/patents/US7329333