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*  Behavioral neuroscience - Wikipedia
Genetically encoded voltage sensitive fluorescent proteins have also been developed. Calcium imaging relies on dyes or ... "Real-time imaging of neurons retrogradely and anterogradely labelled with calcium-sensitive dyes." Journal of Neuroscience ... "Genetically Encoded Indicators of Cellular Calcium Dynamics Based on Troponin C and Green Fluorescent Protein." The Journal of ... doi:10.1016/j.cell.2010.02.037 Ebner, T. J. and Chen, G. "Use of voltage-sensitive dyes and optical recordings in the central ...
  https://en.wikipedia.org/wiki/Behavioral_neuroscience
*  Genetically encoded voltage indicator - Wikipedia
... calcium imaging, or voltage sensitive dyes. It can show neuron signals with subcellular spatial resolution. It has fast ... "Genetically encoded voltage indicators for large scale cortical imaging come of age". Current Opinion in Chemical Biology. 27: ... "Rational optimization and imaging in vivo of a genetically encoded optical voltage reporter". The Journal of Neuroscience. 28 ( ... Genetically encoded voltage indicator (or GEVI) is a protein that can sense membrane potential in a cell and relate the change ...
  https://en.wikipedia.org/wiki/Genetically_encoded_voltage_indicator
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... traditionally using synthetic calcium indicators (e.g. calcium green, FURA-2) or voltage-sensitive dyes (e.g. RH414) - has long ... Development of genetically-encoded neural activity reporters, such as the fluorescent calcium indicators G-CaMP11,12 and ... Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe. Authors: Ana F. Silbering, Rati Bell, C. Giovanni ... The in vivo and semi-in vivo preparation for Calcium imaging has been developed in our lab by Joerges, Küttner and Galizia over ...
  https://www.jove.com/visualize/abstract/21765923/hornets-can-fly-at-night-without-obvious-adaptations-eyes
*  Publications | Max Planck Institute of Neurobiology
... a new family of genetically encoded indicators for in vivo calcium imaging in the nervous system. Cell Calcium 42: 351-61 ... Ng D, Fromherz P (2011). Genetic targeting of a voltage-sensitive dye by enzymatic activation of phosphonooxymethyl-ammonium ... Garaschuk O, Griesbeck O (2009). Monitoring calcium levels with genetically encoded indicators. In: Calcium Measurement Methods ... A genetically encoded calcium indicator for chronic in vivo two photon imaging. Nature Methods 5: 805-811 ...
  http://www.neuro.mpg.de/26870/publications
*  Imaging the Dynamics of Neocortical Population Activity in Behaving and Freely Moving Mammals | SpringerLink
The development of functional imaging techniques applicable to neuroscience and covering a wide range of spatial and temporal ... Slovin H, Strick PL, Hildesheim R, Grinvald A (2003) Voltage sensitive dye imaging in the motor cortex I. Intra- and ... Siegel MS, Isacoff EY (1997) A genetically encoded optical probe of membrane voltage. Neuron 19:735-741PubMedCrossRefGoogle ... Berger T, Borgdorff AJ et al (2007) Combined voltage and calcium epifluorescence imaging in vitro and in vivo reveals ...
  https://link.springer.com/chapter/10.1007/978-1-4419-6558-5_10
*  State-of-the-Art Techniques to Causally Link Neural Plasticity to Functional Recovery in Experimental Stroke Research
C. E. Brown, K. Aminoltejari, H. Erb, I. R. Winship, and T. H. Murphy, "In vivo voltage-sensitive dye imaging in adult mice ... K. Horikawa, "Recent progress in the development of genetically encoded Ca2+ indicators," The Journal of Medical Investigation ... That wide-field calcium imaging might be also a powerful tool to study brain-wide cortical reorganization on a high-resolution ... Another technique to study in particular sensory map shifts is millisecond-timescale voltage-sensitive dye (VSD) imaging which ...
  https://www.hindawi.com/journals/np/2018/3846593/
*  Tracking individual action potentials throughout mammalian axonal arbors | eLife
... voltage-sensitive-dye-based techniques enabled the imaging of individualaction potential propagating into axon collaterals of ... imaging of spikes and synaptic potentials in single neurons in brain slices with genetically encoded hybrid voltage sensor * N ... sensitive to voltage (Peterka et al., 2011) or calcium (Grienberger and Konnerth, 2012), have become popular means for ... Cable properties of a straight neurite of a leech neuron probed by a voltage-sensitive dye * P Fromherz ...
  https://elifesciences.org/articles/30198
*  An Optogenetic Guide to the Brain | Research & Technology | Jul 2010 | Photonics.com
Scientists at RIKEN have developed a genetically encoded fluorescent sensor that can act as a direct optical indicator of ... Other genetically encoded sensors have been developed that respond to calcium flux in the immediate aftermath of neuronal ... Detectorssensory perceptionsomatosensory cortexThomas Knöpfelvoltage potentialvoltage-sensitive fluorescent proteinsVSFPs ... Browse by Subject Lasers Optics Imaging Biophotonics Industrial Sensors & Detectors Test & Measurement Light Sources Fiber ...
  https://www.photonics.com/Articles/An_Optogenetic_Guide_to_the_Brain/a43150
*  Volume 2 Issue 2 | Neurophotonics
Voltage-sensitive dye imaging during functional development of the embryonic nervous system: a brief review with special thanks ... While cutting edge research has trended toward the use of genetically encoded calcium or voltage indicators, two-photon ... TOPICS: Nervous system, Voltage sensitive dyes, Imaging systems, Neurons, Optical recording, Tissues, Nerve, Neural networks, ... All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated ...
  https://www.spiedigitallibrary.org/journals/neurophotonics/volume-2/issue-02
*  Voltage-sensitive dye - Wikipedia
Voltage-sensitive dyes offer the only alternative to genetically encoded voltage sensitive proteins (such as Ci-VSP derived ... March 2005). "Imaging brain activity with voltage- and calcium-sensitive dyes". Cell. Mol. Neurobiol. 25 (2): 245-82. doi: ... The core material for imaging brain activity with voltage-sensitive dyes are the dyes themselves. These voltage-sensitive dyes ... Weaknesses of imaging brain activity with voltage-sensitive dyes include the following problems: Voltage-sensitive dyes may ...
  https://en.wikipedia.org/wiki/Voltage-sensitive_dye
*  'Blue' voltage-sensitive dyes for studying spatiotemporal dynamics in the brain: visualizing cortical...
... about 15 years before the first useful genetically encoded voltage florescent sensors became practically useable. Blue dyes ... Here, we discuss how the blue dyes help to achieve high-sensitivity voltage-sensitive dye imaging of spontaneous and evoked ... J. Lechleiter et al., "Spiral calcium wave propagation and annihilation in Xenopus laevis oocytes," Science 252(5002), 123-126 ... M. T. Lippert et al., "Methods for voltage sensitive dye imaging of rat cortical activity with high signal to noise ratio," J. ...
  https://www.spiedigitallibrary.org/journals/neurophotonics/volume-4/issue-03/031207/Blue-voltage-sensitive-dyes-for-studying-spatiotemporal-dynamics-in-the/10.1117/1.NPh.4.3.031207.full?SSO=1
*  'signal to noise ratio' Protocols and Video...
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors', 'Indoor Experimental Assessment ... Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes', 'Lensless Fluorescent ... Measuring the Calcium Dynamics of Individual, Genetically-labeled Neurons of the Developing Mouse Neocortex', ' ... Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging', 'Imaging Intermediate Filaments and Microtubules ...
  https://www.jove.com/keyword/signal+to+noise+ratio
*  A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices |...
2013) Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics. Front ... Four design strategies have been used to engineer genetically encoded voltage indicators. The first uses a voltage-sensitive ... 1975) Rapid changes in intracellular free calcium concentration: detection by metallochromic indicator dyes in squid giant axon ... 1997) A genetically encoded optical probe of membrane voltage. Neuron 19:735-741, doi:10.1016/S0896-6273(00)80955-1, pmid: ...
  http://www.jneurosci.org/content/36/8/2458.long
*  Mesoscale Transcranial Spontaneous Activity Mapping in GCaMP3 Transgenic Mice Reveals Extensive Reciprocal Connections between...
2009) Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes. Philos Trans R Soc Lond B ... 2013) Two-photon voltage imaging using a genetically encoded voltage indicator. Sci Rep 3:2231, doi:10.1038/srep02231, pmid: ... During the last decade, the emergence of high resolution and sensitive approaches, such as voltage sensitive dye (VSD) imaging ... 2010) Voltage-sensitive dye imaging: technique review and models. J Physiol Paris 104:40-50, doi:10.1016/j.jphysparis.2009.11. ...
  http://www.jneurosci.org/content/34/48/15931?ijkey=c41848128c040c7c1edbb903aac0a6ffc12676cd&keytype2=tf_ipsecsha
*  Intravital Two-Photon Excitation Microscopy in Neuroscience: General Concepts and Applications | SpringerLink
Two-photon voltage imaging using a genetically encoded voltage indicator. Sci Rep 3:2231. doi: 10.1038/srep02231 PubMedCentral ... Kuhn B, Denk W, Bruno RM (2008) In vivo two-photon voltage-sensitive dye imaging reveals top-down control of cortical layers 1 ... Tian L, Hires SA, Looger LL (2012) Imaging neuronal activity with genetically encoded calcium indicators. Cold Spring Harb ... responses in layer 2/3 of rat barrel cortex measured in vivo by voltage-sensitive dye imaging combined with whole-cell voltage ...
  https://link.springer.com/chapter/10.1007/978-94-017-9361-2_1
*  Frontiers | Layer- and column-specific knockout of NMDA receptors in pyramidal neurons of the mouse barrel cortex. | Frontiers...
Neurons transduced with lentivector encoding GFP and Cre recombinase exhibit not only reductions in NMDAR 1 mRNA levels, but ... Neurons transduced with lentivector encoding GFP and Cre recombinase exhibit not only reductions in NMDAR 1 mRNA levels, but ... voltage-sensitive dye imaging in freely moving mice. Neuron 50, 617-629. ... A) pCMVΔR8.92 encoding virion components, pRSV-Rev encoding HIV-1 Rev protein, pMD.G, encoding the VSV-G glycoprotein, and the ...
  https://www.frontiersin.org/articles/10.3389/neuro.07.001.2007/full
*  OSA | Label-free optical detection of action potential in mammalian neurons
L. Tian, S. A. Hires, and L. L. Looger, "Imaging neuronal activity with genetically encoded calcium indicators," Cold Spring ... C. B. Chien and J. Pine, "Voltage-sensitive dye recording of action potentials and synaptic potentials from sympathetic ... L. Tian, S. A. Hires, and L. L. Looger, "Imaging neuronal activity with genetically encoded calcium indicators," Cold Spring ... L. Tian, S. A. Hires, and L. L. Looger, "Imaging neuronal activity with genetically encoded calcium indicators," Cold Spring ...
  https://www.osapublishing.org/boe/abstract.cfm?URI=boe-8-8-3700
*  Aequorin - Wikipedia
It lacks phenomena of intracellular compartmentalization or sequestration as is often seen for Voltage-sensitive dyes, and does ... Heim N, Griesbeck O (2004). "Genetically encoded indicators of cellular calcium dynamics based on troponin C and green ... extending the ability to image calcium transients during later stages of development". Int. J. Dev. Biol. 50 (6): 561-69. doi: ... Such issues led to developments of other genetically encoded calcium sensors including the calmodulin-based sensor cameleon, ...
  https://en.wikipedia.org/wiki/Aequorin
*  'action potentials' Protocols and Video...
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors', 'Murine Short Axis Ventricular ... Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices', 'Multi- ... Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons', 'Voltage and Calcium Dual ... Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons', 'Retrograde Fluorescent ...
  https://www.jove.com/keyword/action+potentials
*  Frontiers | Integrating anatomy and function for zebrafish circuit analysis | Frontiers in Neural Circuits
Advanced stimulation techniques allow the activation of optogenetic actuators at different resolution levels, and genetically ... encoded calcium indicators report the activity of a large proportion of neurons in the CNS. Large datasets result and need to ... Advanced stimulation techniques allow the activation of optogenetic actuators at different resolution levels, and genetically ... encoded calcium indicators report the activity of a large proportion of neurons in the CNS. Large datasets result and need to ...
  https://www.frontiersin.org/articles/10.3389/fncir.2013.00074/full
*  Patent US6814933 - Multiwell scanner and scanning method - Google Patents
... which may be loaded with ion or voltage sensitive dyes to report receptor or ion channel activity, such as calcium channels or ... Emerging Applications of Fluorescence Spectroscopy to Cellular Imaging: Lifetime Imaging, Metal-ligand Probes, Multi-photon ... Cells for this type of assay can be made by co-transfection of a host cell with DNA encoding a cyclic nucleotide-gated ion ... Many cells that can be genetically engineered to express a heterologous cell surface protein are known. Such cells include, but ...
  http://www.google.com/patents/US6814933?dq=6,631,400
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Voltage-sensitive dye imaging of neurons is a key methodology for the understanding of how neuronal networks are organised and ... Two-photon excitation of the calcium-sensitive dye results in high spatial resolution in opaque brain tissue2. Reconstruction ... yet there is still a great deal left to learn about whether and how neuronal populations encode information in a laminar- ... for the purpose of targeting extracellular recordings to genetically-identified cells. Here we provide a set of guidelines for ...
  https://www.jove.com/visualize/abstract/23671583/a-model-based-spike-sorting-algorithm-for-removing-correlation
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and voltage sensitive dye recording. Finally, we demonstrate a method for regionally dissecting subregions of the hippocampus ... Neuroscience, Issue 51, neuronal dissociation, synaptic transmission, GABA, calcium imaging, electrophysiology, hippocampus, ... elegans nrx-1 and nlg-1 genes are orthologous to human NRXN1 and NLGN1 genes which encode alpha-neurexin-1 and neuroligin-1 ... An important advantage of this paradigm is its applicability to studies with genetically-modified mice. Additionally, this ...
  https://www.jove.com/visualize/abstract/22539981/altered-synaptic-properties-during-integration-adult-born-hippocampal
*  Frontiers | Zebrafish: a novel research tool for cardiac (patho)electrophysiology and ion channel disorders | Physiology
... and transmembrane APs may be evaluated using voltage-sensitive dyes (Panáková et al., 2010). Application of these voltage- ... which both encode and form typical voltage-gated Na+ channels/currents (Novak et al., 2006; Chopra et al., 2010). Furthermore, ... These high resolution imaging techniques are powerful tools for the study of zebrafish physiology (Jou et al., 2010), but these ... To date, genetically modified mice have been predominantly used to investigate and model human cardiac diseases, including ...
  https://www.frontiersin.org/articles/10.3389/fphys.2012.00255/full
*  Optogenetics - Wikipedia
"Imaging neuronal activity during zebrafish behavior with a genetically encoded calcium indicator". Journal of Neurophysiology. ... "Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein". J Neurophysiol. 108 (8): 2323-37. doi:10.1152/jn ... Wang, Y.; Dye, C. A.; Sohal, V.; Long, J. E.; Estrada, R. C.; Roztocil, T.; Lufkin, T.; Deisseroth, K.; Baraban, S. C.; ... The primary tools for optogenetic recordings have been genetically encoded calcium indicators (GECIs). The first GECI to be ...
  https://en.wikipedia.org/wiki/Optogenetics