Characterization of expressed sequence tags from a gallus gallus pineal gland cDNA library. Comparative and Functional Genomics ... Characterization of expressed sequence tags from a gallus gallus pineal gland cDNA library. Hartman, S., Touchton, G., Wynn, J ... Characterization of expressed sequence tags from a gallus gallus pineal gland cDNA library. ... we describe the isolation and characterization of expressed sequence tags (ESTs) isolated from a chicken pineal gland cDNA ...

Sudo K, Chinen K, Nakamura Y (Nov 1994). "2058 expressed sequence tags (ESTs) from a human fetal lung cDNA library". Genomics. ... "Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807-28. doi:10.1101/gr.6.9.807. PMID ... end-enriched cDNA library". Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Liu H, Sugiura M, Nava VE ...

These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that ... Results: Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). Aphids for library ... These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that ... Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic ...

PubMed database of biomedical literature with 38 other literature and molecular databases including DNA and protein sequence, ... Expressed Sequence Tag) division of GenBank. These are typically short single-pass reads from cDNA libraries often generated as ... Apart from sequence data in the EST (Expressed Sequence Tag) and GSS (Genome Survey Sequence divisions of GenBank, the ... The SRA (Sequence Read Archive) contains sequencing data from the next generation sequencing platforms. SRA accepts and ...

... a project to generate Aspergilllus flavus Expressed Sequence Tags (ESTs) have been initiated. More than 26,110 cDNA clones have ... Title: Batch Submission and Release of 19,618 Aspergillus flavus Expressed Sequence Tags (ESTs) Author. Yu, Jiujiang ... Aspergillus flavus expressed sequenced tags (ESTs) provides a rapid and effective method for identification of the genes ... Batch Submission and Release of 19,618 Aspergillus flavus Expressed Sequence Tags (EST). National Center for Biotechnology ...

"Construction of cDNA libraries from Protists and Fungi." In: Expressed sequence tags - generation and analysis, Methods in ... "TBestDB: a taxonomically broad database of expressed sequence tags (ESTs)." Nucleic Acids Res 35:D445-451 Abstract 2006 Iwatani ... In: Expressed sequence tags - generation and analysis, Methods in Molecular Biology Vol. 533 (J. Parkinson, ed.), Humana Press ... "Subunit 1 of cytochrome oxidase from Neurospora crassa: Nucleotide sequence of the coding gene and partial amino acid sequence ...

report the genome sequence of the laboratory classic A. nidulans, and Nierman et al. have sequenced A. fumigatus, known chiefly ... present genome sequencing and analysis of A. oryzae, focusing in particular on the expansion of genes in its genome, which is ... The blocks of A. oryzae-specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis ... has been sequenced. The ability to secrete large amounts of proteins and the development of a transformation system1 have ...

... fragmented and hybridized to HuGeneFL GeneChip probe arrays representing more than 6800 human genes and expressed sequence tags ... Total RNA was used for the preparation of double stranded cDNA. Biotin labeled cRNA transcripts were synthesized, ...

METHODS: To identify expressed TNFSF genes, we searched a computer database of ~50,000 rainbow trout expressed sequence tags ... RESULTS: Here, we report the characterization of a cDNA, 1RT126I22, which contains a TNF homology domain. Phylogenetic analyses ... Trout TNFSF 13b RNA is expressed in immune tissues including the spleen, anterior kidney, and peripheral blood leukocytes but ... Three introns were identified in the rainbow trout, Atlantic salmon and zebrafish genomic sequences, and the intron locations ...

BACKGROUND: Large-scale partial sequencing of cDNA libraries to generate expressed sequence tags (ESTs) is an effective means ... expressed sequence tags upon hSNF5/INI1 induction [4].. *Recently, sequencing of a human expressed sequence tag, which maps ... Associations of Expressed Sequence Tags with chemical compounds. *We identified an expressed sequence tag clone that shared ... Gene context of Expressed Sequence Tags. *However, analysis of expressed sequence tag (EST) databases indicated that there ...

The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine ... cDNA). Incorporation was traced using ornithine labeled with radioactive carbon (14C). ... Periportal hepatocytes express these enzymes; epithelial cells of the small intestine and kidney also express these enzymes to ... The carrier protein and gene sequence have only recently been identified; before its identification, the carriers dysfunction ...

Random Expressed Sequence Tag (EST) clones have been isolated and sequenced from dolphin Peripheral Blood Leukocyte (PBL) cDNA ... From the two cDNA-libraries 24,000 ESTs were collected and a total of 2200 unigenes have been sequenced and annotated (www. ... Two cDNA libraries from known health status dolphin PBL have been generated including an IL-2 and an LPS-stimulated cDNA ... together with other genes randomly selected without sequencing from the cDNA libraries, have been amplified and used to ...

Differential Analysis of Expressed Sequence Tags from SMART cDNA Plasmid Libraries of the Orange-spotted Grouper ( Epinephelus ... Phylogeny of Some Muscicapinae Genera and Species Inferred from Mitochondrial Gene COI Sequences LEI Xin; LIAN Zhen-min; LEI Fu ...

... expressed sequence tag (EST), cDNA and expression databases for a new gene named SPRN (shadow of prion protein). SPRN comprises ... We report evidence from cDNA isolation and expression analysis as well as analyses of genome, ... We report evidence from cDNA isolation and expression analysis as well as analyses of genome, expressed sequence tag (EST), ... Sequence comparison shows: a highly conserved N-terminal signal sequence; Arg-rich basic region containing up to six ...

Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse ... Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low ... Based on random, high-throughput EST (expressed sequence tag) sequencing, a clone from a cDNA library generated from a donor in ... that express SCF, GM-CSF, and IL-3 (NSG-SGM3) generate human mast cells and eosinophils that express functional Siglec-8 [35]. ...

The UniGem V 2.0 array contains 8,502 unique genes and expressed sequence tags (ESTs). At first, we analyzed the array for ... and the blots were hybridized with cDNA probes. The following probes were used: 2.12-kB p21 WAF1 cDNA fragment from pZL-WAF1 ( ... cDNA microarray analysis. Total RNA from NB4 cells cultured for 6 hours with RA (1 μM) and SAHA (0.9 μM) alone and in ... The membrane was probed by using 32P-random labeled cDNA probes as indicated. As a loading control, the same membranes were ...

A polymerase chain reaction-based subtractive hybridization procedure was applied to cDNAs prepared from mouse insulinoma (βTC3 ... 17 were identical or homologous to known mammalian cDNAs or expressed sequence tags. Genes known to be highly expressed in β- ... cell lines to construct a library of cDNAs that are highly expressed in pancreatic β-cells. An analysis of 555 randomly chosen ... Many of the novel cDNA sequences that were highly represented in the library showed a relative specificity to β-cells compared ...

The short cDNA sequence Fragments when making this method are called expressed sequence tags , or ESTs. The NIH Office of ... "Patent Law Utility Federal Circuit holds therein expressed sequence tags Lack Substantial and specific utility unless ... The browser zorgt a comparative view tussen NCBI and HuRef consensus sequences, the sequence multi-alignment of the HuRef ... The HuRef database consists of ongeveer 32 million DNA reads sequenced using microfluidic Sanger sequencing , assembled JSON ...

Expressed Sequence Tags (ESTs), in search of tumor- specific and tumor- associated antigens. The public databases currently ... The experimental part centers on mRNA expression profiling (with cDNA microarrays, oligonucleotide chips, and RT-PCR), ... al Open- reading- frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans. Nature Genetics 27 ( ... embryogenomics: Fundamental questions in developmental biology are: what genes are expressed, where and when they are expressed ...

These progressed from Sanger sequencing of Expressed Sequence Tag libraries, to chemical tag-based methods (e.g., serial ... cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned ... in situ sequencing of fixed tissue, and native RNA molecule sequencing with single-molecule real-time sequencing. Prior to RNA- ... and needing to know the sequence a priori. Because of these technical issues, transcriptomics transitioned to sequencing-based ...

The best-aligned sequences were annotated with gene ontology groups. When comparing the transcriptomes across developmental ... 3168 expressed sequence tags (ESTs) were selected and sequenced from a cDNA library of NaHCO3-treated C. virgata, based on ... cDNA Synthesis and RNA-Seq Analysis Using an Illumina Sequencing System. To create a cDNA sequencing library, mRNAs with poly ( ... cDNA Synthesis and RNA-seq Analysis by Ion Proton Sequencing. The Thermo Fisher Ion ProtonTM sequencing system was used for RNA ...

... and Strep-tag II-encoding sequences. Clones were mutagenized when indicated, expressed, and purified as described (24). ... We cloned codon-optimized H5-encoding cDNAs (GenScript, Piscataway, NJ, USA) of H5N1 A/wild duck/Hunan/211/2005 (GenBank ... Gu M, Huang J, Chen Y, Chen J, Wang X, Liu X, et al. Genome sequence of a natural reassortant H5N2 avian influenza virus from ... Whole-genome sequence of a reassortant H5N6 avian influenza virus isolated from a live poultry market in China, 2013. Genome ...

... which contained probe sets of over 22 047 known transcripts and expressed sequence tags. Gene Chips were washed and stained in ... After labeling, fragmented cDNA was hybridized for 16 h at 45°C on the Gene Chip Affymetrix® OviGene-1_0-ST Array (Affymetrix ... Table 1. Real-time PCR primer sequence.. Table 2. Representative differentially expressed genes (DEGs) between fibrous and bony ... Table 1. Real-time PCR primer sequence.. Table 2. Representative differentially expressed genes (DEGs) between fibrous and bony ...

expressed sequence tag. hPAHT. human p-aminohippurate transporter. kb. kilobase pair. oatp. organic anion transporting ... Cloning of hOAT1 cDNA.. To identify potential cDNA sequences encoding a hOAT homolog, a BLAST search of the GenBank EST ... A, complete nucleotide sequence of the sense strand of the hOAT1 cDNA and the predicted amino acid sequence of the single large ... DNA Sequencing and Analysis.. The clone containing the largest cDNA insert (pOAT-8) was subjected to complete sequence analysis ...

BES analyses identified 6,848 simple sequence repeats (SSRs), of which 3,854 had high quality flanking sequences for PCR ... we sequenced a large number of rainbow trout BAC-end sequences (BES) and characterized their contents. A total of 176,485 high ... aid in future assembly of a reference genome sequence and elucidating sequence content and complexity in the rainbow trout ... However, the lack of a reference genome sequence hampers research progress for both academic and applied purposes. In order to ...

Expressed Sequence Tag (EST) （表达序列标签） Randomly selected, partial cDNA sequence; represents its corresponding mRNA. dbEST is a ... See Expressed Sequence Tag. Expect value (E)（E值） E value. The number of different alignents with scores equivalent to or better ... Expectation maximization (sequence analysis) An algorithm for locating similar sequence patterns in a set of sequences. A ... the one found between a query sequence and a database sequence would be found in as many comparisons between random sequences ...

... a catfish EST sequencing project was selected by the DOEs Joint Genome Institute (JGI). In this project, a total of 12 cDNA ... Expressed Sequence Tag (EST) sequencing is one of the most efficient means for gene discovery and gene expression profiling. ... Assembly of 500,000 Inter-Specific Catfish Expressed Sequence Tags and Large Scale Gene-Associated Marker Development for ... The JGI EST sequencing had an overall sequencing success rate of 73% with an average length of 576 bp. All the ESTs were ...

... expressed sequence tags; short single pass cDNA sequences GSS; genome survey sequences; short single pass genomic sequences TSA ... expressed sequence tags) ddbjgss; Category for GSS (genome survey sequences) ddbjhtc; Category for HTC (high throughput cDNA ... The sequence submitted mainly from genome sequencing projects which regarded a clone as a sequencing unit. STS; sequence tagged ... high throughput cDNA sequences; The sequence submitted from cDNA sequencing projects except for EST. This division is to ...

Our results motivate broad sampling of additional genome sequences from these phyla. ... Methods in Molecular Biology: Expressed Sequence Tags (ESTs). Edited by: Parkinson J. 2009, Totowa, NJ: Humana Press, 533:. ... Construction of cDNA libraries and EST sequencing. Two N. simplex (CCAP 1552/2 and 1552/4) cDNA libraries were constructed ... Methods in Molecular Biology: Expressed Sequence Tags (ESTs). Edited by: Parkinson J. 2009, Totowa, NJ: Humana Press, 433:. ...