Bulky DNA adducts and lung cancer risk: a prospective study in EPIC investigation | Cancer Research
Leukocyte DNA adducts were analysed blindly using the 32P DNA postlabelling technique. The intensity of adduct patterns was ... Bulky DNA adducts and lung cancer risk: a prospective study in EPIC investigation. Marco Peluso, Armelle Munnia, Domenico Palli ... Our large prospective study suggests that the presence of bulky 32P-postlabelling DNA adducts in the WBC of non-smokers may ... Therefore, we have measured bulky 32P-postlabelling DNA adducts in a case-control study nested in a large prospective ...http://cancerres.aacrjournals.org/content/65/9_Supplement/512.2
Abstract IA18: The post-transcriptional response to bulky-adduct DNA damage | Cancer Research
Abstract IA18: The post-transcriptional response to bulky-adduct DNA damage Message Subject (Your Name) has forwarded a page to ... For example, we have shown that following treatment with agents that cause bulky-adduct DNA damage, such a platinum-based ... Abstract IA18: The post-transcriptional response to bulky-adduct DNA damage. Anne E. Willis ... The post-transcriptional response to bulky-adduct DNA damage. [abstract]. In: Proceedings of the AACR Special Conference on ...http://cancerres.aacrjournals.org/content/77/6_Supplement/IA18
Cancer Flashcards by Tina Jaramillo | Brainscape
3) bulky DNA adducts that can interefere with base pairing. 4) double strand breaks ... detection of DNA damage. repar of damaged DNA. inactivation of mutagenic molecules. telomere maintenance ... accidents! during DNA replication. mechanisms:. oxidative damage. external agents (chemicals, virus, radiation) ... defect in DNA mismatch repair linked to MLH1,MSH2, and related genes ...https://www.brainscape.com/flashcards/cancer-2387782/packs/4218896
Department of Chemistry and Biochemistry - University of Maryland, College Park MD Calendar - Department of Chemistry and...
New Mechanisms for Repair of Bulky DNA Adducts and Interstrand DNA Crosslinks. 11:00 am: New Mechanisms for Repair of Bulky DNA ... Send New Mechanisms for Repair of Bulky DNA Adducts and Interstrand DNA Crosslinks to Google Calendar ... More about New Mechanisms for Repair of Bulky DNA Adducts and Interstrand DNA Crosslinks ... Adducts and Interstrand DNA Crosslinks. February 1, 2018 11:00 am. - 12:00 pm. ...http://www.chem.umd.edu/whats-new/calendar?yr=2018&month=1&dy=&cid=mc-04334768eac9c6ddaf9c14dfad1a6b8e
Occupational and Environmental Medicine | Research on the chemical, physical, ergonomic, biological and psychosocial hazards in...
Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to ...https://oem.bmj.com/
September 2012 - Volume 23 - Issue 5S : Epidemiology
O-012: Bulky DNA Adducts in Cord Blood, Maternal Diet and Birth Weight in a European Prospective Mother-Child Study (NEWGENERIS ... E-013: Follow-up Investigation in Oxidative DNA Damage and Lipid Peroxidation 3 Years after Hebei Spirit Oil Spill. Noh, Su ...https://journals.lww.com/epidem/toc/2012/09001
Polycyclic aromatic hydrocarbon - Wikipedia
... forming bulky complexes called DNA adducts that can be stable or unstable. Stable adducts may lead to DNA replication ... An adduct formed between a DNA strand and an epoxide derived from a benzo[a]pyrene molecule (center); such adducts may ... Bulky DNA Adducts and Cellular Responses". In Luch, A. Molecular, Clinical and Environmental Toxicology. Experientia ... This enzyme then metabolically processes the PAHs to quinone metabolites that bind to DNA in reactive adducts that remove ...https://en.wikipedia.org/wiki/Polycyclic_aromatic_hydrocarbon
Current Projects: Geography | Damon Runyon
Replicative helicase bypass of bulky DNA adducts?' with Johannes C. Walter, PhD VIEW PROJECT. ... Mechanism of DNA processing during non-homologous end joining' with Johannes C. Walter, PhD, and Joseph L. Loparo, PhD VIEW ... DNA methylation stochasticity in pediatric pre-B cell acute lymphoblastic leukemia' with Andrew P. Feinberg, MD VIEW PROJECT. ... Global analysis of DNA break repair by single-cell sequencing' with Jay A. Shendure, MD, PhD VIEW PROJECT. ...https://www.damonrunyon.org/our-impact/current-projects/geography/HI
Current Projects: Geography | Damon Runyon
Replicative helicase bypass of bulky DNA adducts?' with Johannes C. Walter, PhD VIEW PROJECT. ... Mechanisms that promote DNA double strand break clusters in brain and liver with Frederick W. Alt, PhD VIEW PROJECT. ... Mechanism of DNA processing during non-homologous end joining' with Johannes C. Walter, PhD, and Joseph L. Loparo, PhD VIEW ... DNA methylation stochasticity in pediatric pre-B cell acute lymphoblastic leukemia' with Andrew P. Feinberg, MD VIEW PROJECT. ...https://www.damonrunyon.org/our-impact/current-projects/geography/AZ
Arkiv for Publikasjoner - Side 2 av 51 - STAMI
Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to ...https://stami.no/publikasjon/page/2/
Genetic variation in the base excision repair pathway and bladder cancer risk - Semantic Scholar
SNPs in three genes showed significant associations with bladder cancer risk: the 8-oxoG DNA glycosylase gene (OGG1), the Poly ... Genetic polymorphisms in DNA repair genes may impact individual variation in DNA repair capacity and alter cancer risk. In ... DNA repair gene polymorphisms, bulky DNA adducts in white blood cells and bladder cancer in a case-control study.. *Giuseppe ... Genetic polymorphisms in DNA repair genes may impact individual variation in DNA repair capacity and alter cancer risk. In ...https://www.semanticscholar.org/paper/Genetic-variation-in-the-base-excision-repair-and-Figueroa-Malats/d9e1b0ef7a31b0a5a6ab7b948f75bb886ca6a99b
Mutagenicity and antimutagenicity of (−)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet...
However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used. ... The mutagenicity∕genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA ... bulky stable and depurinating DNA adducts are formed [33, 34]. Insufficient removal of these DNA adducts prior to replication ... The exo-epoxide form of AFB1 binds to DNA to form the predominant 8,9-dihydro- 8-(N7-guanyl)-9-hydroxy AFB1 adduct, leading to ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-12-203
Repair of mitomycin C mono- and interstrand cross-linked DNA adducts by UvrABC: a new model
... which is similar to what occurs with other bulky DNA adducts, such as benzo(a)pyrene diol epoxide-dG adducts and cyclobutane ... A) Chemical structures of MC, MC-mono-dG, and ICL dG-MC-dG DNA adducts. (B) The DNA sequence of the 61-bp DNA fragment ... In order to obtain definitive UvrABC-DNA adduct interactions 61-bp DNA fragments containing a site-specific MC-mono-dG adduct ( ... kink in the DNA helix (17). Similar to the ICL dG-MC-dG adduct, it has been shown that an MC-mono-dG adduct does not induce DNA ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2978355/
CHRNA5 | Cancer Genetics Web
Functional effect of polymorphisms in 15q25 locus on CHRNA5 mRNA, bulky DNA adducts and TP53 mutations.. Int J Cancer. 2013; ... polycyclic aromatic hydrocarbon-DNA adduct levels, (iii) TP53 mutations and (iv) susceptibility to lung cancer. ... were associated with differential levels of genetic alterations measured as the levels of hydrophobic DNA adducts in the ... The two most frequent haplotypes were associated with the risk of developing lung cancer and with smoking-related DNA ...http://www.cancerindex.org/geneweb/CHRNA5.htm
Inter Research » MEPS » v205 » p195-206
Regardless of the exposure dose of B[a]P, several bulky DNA adducts were induced in the typical PAH diagonal radioactive zone. ... Induction and elimination of bulky benzo[a]pyrene-related DNA adducts and 8 oxodGuo in mussels Mytilus galloprovincialis ... DNA repair during depuration differed among the B[a]P-induced DNA adducts. B[a]P exposure had no effect on gill 8-oxodGuo ... KEY WORDS: B[a]P · Mussel · DNA adducts · 8-oxodGuo · DNA repair ...http://www.int-res.com/abstracts/meps/v205/p195-206/
Probing for DNA damage with beta-hairpins: similarities in incision efficiencies of bulky DNA adducts by prokaryotic and human ... Effect of ring size on conformations of aromatic amine-DNA adducts: the aniline-C8 guanine adduct resides in the B-DNA major ... temperature effects on nucleotide incorporation opposite a bulky carcinogen-DNA adduct by a Y-family DNA polymerase.. J Biol ... Thermodynamic and structural factors in the removal of bulky DNA adducts by the nucleotide excision repair machinery.. ...https://as.nyu.edu/faculty/suse-broyde.html
IJERPH | Free Full-Text | Biomarkers of Induced Active and Passive Smoking Damage | HTML
Levels of DNA adducts measured in many tissues of smokers are significantly higher than in non-smokers. While some studies have ... In order to establish whether smoking-related DNA adducts are biomarkers of tobacco smoke exposure and/or its carcinogenic ... activity we summarized all data that associated tobacco smoke exposure and smoking-related DNA adducts both in controls and/or ... demonstrated an association between carcinogenic DNA adducts and cancer in current smokers, no association has been observed in ...http://www.mdpi.com/1660-4601/6/3/874/htm
rs1042522 - SNPedia
PMID 24084248] The GSTM1null (deletion) and MGMT84 rs12917 (Phe/Phe) haplotype are associated with bulky DNA adduct levels in ... Polymorphisms in DNA-Repair Genes in a Cohort of Prostate Cancer Patients from Different Areas in Spain: Heterogeneity between ... PMID 21029772] Genomic DNA extraction from whole blood stored from 15- to 30-years at -20 degrees C by rapid phenol-chloroform ... PMID 28351583] Candidate apoptotic and DNA repair gene approach confirms involvement of ERCC1, ERCC5, TP53 and MDM2 in ...https://snpedia.com/index.php/Rs1042522
XPC (gene) - Wikipedia
XPC is involved in the recognition of bulky DNA adducts in nucleotide excision repair. It is located on chromosome 3. This gene ... If DNA repair is deficient, DNA damage tends to accumulate. Such excess DNA damage may increase mutations due to error-prone ... Bernstein C, Prasad AR, Nfonsam V, Bernstein H. (2013). DNA Damage, DNA Repair and Cancer, New Research Directions in DNA ... www.intechopen.com/books/new-research-directions-in-dna-repair/dna-damage-dna-repair-and-cancer Harper JW, Elledge SJ (2007). " ...https://en.wikipedia.org/wiki/XPC_(gene)
Epigenetic Modifications of Nucleotide Excision Repair Genes in Oral Squamous Cell Carcinoma | OMICS International
Genotoxic exposure to tobacco carcinogens resulting in DNA damage is an important mechanism of oral squamous cell carcinoma ( ... Nucleotide Excision Repair (NER) pathway removes bulky DNA adducts, generated from tobacco exposure thereby playing a major ... 2002) The XPD variant alleles are associated with increased aromatic DNA adduct level and lung cancer risk. Carcinogenesis 23: ... Since effective DNA repair is at backbone of cancer free survival and a DNA repair system may be modulated in effectiveness by ...https://www.omicsonline.org/open-access/spectrophotometric-determination-of-nifedipine-and-nicardipine-in-theirpharmaceutical-preparations-2469-9764-1000103.php?aid=62308
GSTA1 Antibody (monoclonal) (M08) - Mouse monoclonal antibody raised against a full-length recombinant GSTA1. WB, E - Buy Now!...
PMID 20437058.Lifestyle, environmental, and genetic predictors of bulky DNA adducts in a study population nested within a ...http://www.abgent.com/products/AT2276a-GSTA1-Antibody-monoclonal-M08
Slow repair of bulky DNA adducts along the nontranscribed strand of the human p53 gene may explain the strand bias of ... a presence of bulky DNA adducts.. Finally, the origin of such a genotoxic signature remains to be elucidated. We can ... expression of four base excision DNA repair genes, markers of oxidative DNA damage (29), or 4-hydroxynonenal protein adducts, a ... HNF1α protein recognizes specific palindromic nucleotide DNA sequences and interacts with DNA as either a homodimer or a ...http://diabetes.diabetesjournals.org/content/59/7/1836
rs1048943 - SNPedia
PMID 24084248] The GSTM1null (deletion) and MGMT84 rs12917 (Phe/Phe) haplotype are associated with bulky DNA adduct levels in ... Variation in PAH-related DNA adduct levels among non-smokers: the role of multiple genetic polymorphisms and nucleotide ... metabolism and conjugation genes and PAH-DNA adducts in prostate tumors differ by race. ... PMID 19267064] [Relationship between genetic polymorphisms of phase I and phase II metabolizing enzymes and DNA damage of ...https://snpedia.com/index.php/Rs1048943
rs1799814 - SNPedia
... haplotype are associated with bulky DNA adduct levels in human leukocytes [PMID 23175176. ] Variation in PAH-related DNA adduct ...https://snpedia.com/index.php/Rs1799814
Detection of 1,N2-propanodeoxyguanosine adducts of 2-hexenal in organs of Fischer 344 rats by a 32P-post-labeling technique :...
2-Hexenal is an α,β-unsaturated carbonyl compound which is mutagenic, genotoxic and forms cyclic 1,N2-propanodeoxyguanosine adducts like similar propenals for which carcinogenicity was shown, e.g. acrolein or crotonaldehyde. Since humans have a permanent intake of 2-hexenal via vegetarian food this genotoxic compound is considered to play a role in human carcinogenicity. The data base is, however, presently not sufficient for a cancer risk assessment. To date no long term carcinogenicity study on 2-hexenal has been published. Detection of respective DNA adducts of this substance in animals or humans could allow cancer risk assessment. Therefore, we have developed a 32P-post-labeling technique based on nuclease P1 enrichment and TLC separation of the labeled adducts. The respective adducts are stable over a wide pH range from pH 4 to pH 11 ...http://oxfordindex.oup.com/view/10.1093/carcin/20.7.1345
NIOSHTIC-2 Publications Search - 20029438 - Chlorophyllin modulates gene expression and DNA-adduct formation in normal human...
A panel of NHMEC strains (n=10), each derived from a different donor, was developed from tissue discarded at reduction mammoplasty (Cooperative Human Tissue Network, sponsored by the NCI and the National Disease Research Interchange) and used to study gene expression and DNA adduct formation following exposure to BP (4 M) in the presence or absence of chlorophyllin (5 M). Chlorophyllin treatment hhttps://www.cdc.gov/niosh/nioshtic-2/20029438.html
Formation and Repair of Tobacco Carcinogen-Derived Bulky DNA Adducts (Journal Article) | DOE PAGES
DNA adducts play a central role in chemical carcinogenesis. The analysis of formation and repair of smoking-related DNA adducts remains particularly challenging as both smokers and nonsmokers exposed to smoke are repetitively under attack from complex mixtures of carcinogens such as polycyclic aromatic hydrocarbons and N -nitrosamines. The bulky DNA adducts, which usually have complex structure, are particularly important because of their biological relevance. Several known cellular DNA repair pathways have been known to operate in human cells on specific types of bulky DNA adducts, for example, nucleotide excision repair, base excision repair, and direct reversal involving ...https://www.osti.gov/pages/biblio/1198159-formation-repair-tobacco-carcinogen-derived-bulky-dna-adducts
Variants of mouse DNA polymerase κ reveal a mechanism of efficient and accurate translesion synthesis past a benzo[a]pyrene dG...
Variants of mouse DNA polymerase κ reveal a mechanism of efficient and accurate translesion synthesis past a benzo[a]pyrene dG adduct ...http://sourcedb.big.cas.cn/zw/lwk/201404/t20140410_4088318.html
Metabolic activation of benzo[a]pyrene in vitro by hepatic cytochrome P450 contrasts with detoxification in vivo: experiments...
Many studies using mammalian cellular and subcellular systems have demonstrated that polycyclic aromatic hydrocarbons, including benzo[a]pyrene (BaP), are metabolically activated by cytochrome P450s (CYPs). In order to evaluate the role of hepatic versus extra-hepatic metabolism of BaP and its pharmacokinetics, we used the hepatic cytochrome P450 reductase null (HRN) mouse model, in which cytochrome P450 oxidoreductase, the unique electron donor to CYPs, is deleted specifically in hepatocytes, resulting in the loss of essentially all hepatic CYP function. HRN and wild-type (WT) mice were treated intraperitoneally (i.p.) with 125 mg/kg body wt BaP daily for up to 5 days. Clearance of BaP from blood was analysed by high-performance liquid chromatography with fluorescence detection. DNA adduct levels were measured by 32P-post-labelling analysis with structural confirmation of the formation of 10-(deoxyguanosin-N2-yl)-7,8,9-trihydroxy-7,8,9,10-tetrahydrobenzo[a]pyrene by liquid ...http://oxfordindex.oup.com/view/10.1093/carcin/bgn002
Institute of Cancer Research Repository - Analysis of tamoxifen-DNA adducts in endometrial explants by MS and P-32-postlabeling
Beland, F. A., Churchwell, M. I., Hewer, A., Phillips, D. H., da Costa, G. G., Marques, M. M. (2004) Analysis of tamoxifen-DNA adducts in endometrial explants by MS and P-32-postlabeling. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 320 (2). pp. 297-302. ISSN 0006-291X ...http://publications.icr.ac.uk/1997/
Title page for ETD etd-03122008-133135
Multiple exocyclic DNA adducts arise from reactions of lipid and DNA peroxidation products with DNA bases. These endogenous lesions are mutagenic; therefore, monitoring adduct levels may provide a means of assessing genomic exposure to oxidative damage in human populations. The metabolic processing of endogenously formed exocyclic DNA adducts has now been investigated for the purpose of developing non-invasive markers of oxidative damage. The pyrimidopurinone deoxynucleoside adduct, M1dG, was found to undergo enzymatic oxidation to produce 6-oxo-M1dG. The corresponding base adduct, M1G, was subject to sequential oxidation producing first 6-oxo-M1G and then 2,6-dioxo-M1G. The enzymes xanthine oxidoreductase (XOR) and aldehyde oxidase (AO) catalyzed the oxidation of M1dG and M1G. Additionally, the unsubstitued ...http://etd.library.vanderbilt.edu/available/etd-03122008-133135/
XRCC1, XRCC3, XPD gene polymorphisms, smoking and 32P-DNA adducts in a sample of healthy subjects : Carcinogenesis - oi
DNA repair genes have an important role in protecting individuals from cancer-causing agents. Polymorphisms in several DNA repair genes have been identified and individuals with non-dramatic reductions in the capacity to repair DNA damage are observed in the population, but the impact of specific genetic variants on repair phenotype and cancer risk has not yet been clarified. In 308 healthy Italian individuals belonging to the prospective European project EPIC, we have investigated the relationship between DNA damage, as measured by 32P-DNA adduct levels, and three genetic polymorphisms in different repair genes: XRCC1-Arg399Gln (exon 10), XRCC3-Thr241Met (exon 7) and XPD-Lys751Gln (exon 23). DNA adduct levels were measured as relative adduct level (RAL) per 109 normal nucleotides by ...http://oxfordindex.oup.com/view/10.1093/carcin/22.9.1437
Institute of Cancer Research Repository - Resveratrol inhibits benzo[a]pyrene-DNA adduct formation in human bronchial...
Resveratrol inhibits benzo[a]pyrene-DNA adduct formation in human bronchial epithelial cells Resveratrol (trans-3,4',5-trihydroxystilbene), a phytoalexin present in various plants and foods, has in several in vitro and in vivo studies demonstrated cancer chemopreventive and chemotherapeutic potential. We investigated the in vitro effect of resveratrol on benzo[a]pyrene (B[a]P) -induced DNA adducts in human bronchial epithelial cells. This was compared to the effect of resveratrol on the expression of the cytochrome P450 (CYP) genes CYP1A1 and CYP1B1 and the formation of B[a]P metabolites. Exposure of BEAS-2B and BEP2D cells to B[a]P and increasing concentrations of resveratrol resulted in a dose- and time-dependent inhibition of DNA adduct formation quantified by (32)P-postlabelling. Supporting this result, resveratrol was shown to inhibit CYP1A1 and CYP1B1 gene expression, ...http://publications.icr.ac.uk/1946/
Characterisation of the DNA damage recognised by a monoclonal antibody raised against cisplatin-modified DNA - ePrints -...
Full text for this publication is not currently held within this repository. Alternative links are provided below where available. ...http://eprint.ncl.ac.uk/pub_details2.aspx?pub_id=95905
1887 The 32P-postlabeling assay is an extremely sensitive technique for detecting carcinogen-DNA adducts. However, for the assignment of DNA adduct structures and the accurate determination of DNA adduct levels by 32P-postlabeling authentic adduct standards are needed. For most 32P-postlabeling applications such verified synthetic standard compounds are required in the form of their deoxynucleoside 3′-phosphates because they serve as substrates for the polynucleotide kinase for transfer of [32P]phosphate from [γ-32P]ATP. Three N-(deoxyguanosin)-4-aminobiphenyl 3'-phosphate adducts were prepared and fully characterized by proton NMR and mass spectroscopy to serve as standards for the 32P-postlabeling assay. Apart from the C8- and the N2-deoxyguanosine 3′-phosphate adducts of the mutagenic human bladder ...http://cancerres.aacrjournals.org/content/64/7_Supplement/433.4
Detection of DNA-reactive metabolites in serum and their tissue distribution in mice exposed to multiple...
Our previous studies suggested that body fluids such as serum can serve as a novel surrogate for DNA-containing tissues to overcome the major limiting factor of tissue availability in human biomonitoring assessments. We have now examined the detectability of DNA-reactive metabolites (DRMs) in the serum and tissues of male C57BL/6 mice administered up to 10 i.p. injections on alternate days of individual polynuclear aromatic hydrocarbons (PAHs) or a mixture containing 0.01, 0.1, 1.0 and 5.0 mg/kg body wt each of benzo[a]pyrene (BP), cyclopenta[cd]pyrene (CPP) and benzo[k]fluoranthene (BF) in 200μl sunflower oil. Four hours after the last dose, blood serum was separated and incubated with salmon testes DNA (st-DNA).32P-Post-labeling of the st-DNA showed essentially the same adduct pattern as found in the tissue ...http://oxfordindex.oup.com/view/10.1093/carcin/17.10.2213
Patent US5616700 - Processes for synthesizing nucleotides and modified nucleotides using N.sub. - Google Patents
Disclosed herein are protecting groups for exocyclic amino groups of the base cytosine for use in the synthesis of oligonucleotides and oligonucleoside phosphorothioates, the protecting groups being represented by the formula: --CO--(CH2)0-9 --CH3. In a particularly preferred embodiment, the base cytosine is protected with acetyl (--CO--CH3), and the oligonucleotide or oligonucleoside phosphorothioate incorporating the protected cytosine is subjected to a cleavage/deprotection reagent comprising methylamine and ammonia.http://www.google.com/patents/US5616700?dq=12/011,425
Title page for ETD etd-03282006-151913
The chemistry of acrolein and crotonaldehyde-derived propano-deoxyguanosine (γ-OH-PdG and α-CH3-γ-OH-PdG) adducts was monitored in the 5´-CpG-3´ sequence within a dodecamer duplex by NMR spectroscopy, in situ, using a series of site-specific 13C- and 15N-edited experiments. One striking phenomenon was the formation of an interstrand DNA cross-link, predominantly a carbinolamine. The cross-link existed in equilibrium with the non-crosslinked aldehyde and its geminal diol hydrate. The ratio of aldehyde/diol increased at higher temperatures. The effects of the pH and complementary bases were examined. Molecular modeling suggested that the carbinolamine linkage should be capable of maintaining Watson-Crick hydrogen bonds at both of the tandem C•G base pairs. In contrast, dehydration of the carbinolamine cross-link to an imine (Schiff base) cross-link, or cyclization of the latter to form a pyrimidopurinone cross-link, was predicted to require ...http://etd.library.vanderbilt.edu/available/etd-03282006-151913/
1184 An increased risk of developing endometrial cancer has been observed in women receiving tamoxifen (TAM) endocrine therapy and chemoprevention. The genotoxic damage induced by TAM metabolites may be involved in the development of endometrial cancer. To investigate the capability of forming TAM-DNA adducts in the endometrium, primary cultured human endometrial explants were exposed to α-hydroxytamoxifen (α-OHTAM) and used for quantitative analysis of TAM-DNA adducts, using 32P-postlabeling/polyacrylamide gel electrophoresis and 32P-postlabeling/HPLC analyses. A trans-isoform of α-(N2-deoxyguanosinyl)tamoxifen (dG-N2-TAM) was detected as the major adduct in eight of 9 endometrial explants exposed to 100 μM α-OHTAM at levels of 6.9 ± 5.6 (mean ± S.D.) adducts/107 nucleotides. Approximately 40-fold and 100-fold lower amounts of the cis-form ...http://cancerres.aacrjournals.org/content/65/9_Supplement/276.1
Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to...
Ethenobases are promutagenic DNA adducts formed by some environmental carcinogens and products of endogenous lipid peroxidation. Mutation spectra in tumors induced in mice by urethane or its metabolite vinyl carbamate (Vcar) are compatible with 1,N(6)-ethenoadenine (epsilonA) being an initiating lesion in the development of these tumors. As alkylpurine-DNA-N-glycosylase (APNG) releases epsilonA from DNA in vitro, wild-type and APNG-/- C57Bl/6J mice were treated with Vcar and levels of epsilonA and 3,N(4)-ethenocytosine (epsilonC), which is not a substrate of APNG, were analyzed in liver and lung DNA. At 6 h after the last dose, levels of epsilonA were 1.6-fold higher in DNA from APNG-/- mice and subsequently persisted at higher levels for longer than in DNA from wild-type ...http://christie.openrepository.com/christie/handle/10541/82217
DSpace@MIT: Browsing Department of Chemistry by Title
High mobility group box (HMGB) proteins are DNA-binding proteins that regulate many important DNA-related processes. They are known to recognize the major lesion present in cisplatin-modified DNA, and have been assumed to ...https://dspace.mit.edu/handle/1721.1/7792/browse?rpp=20&order=ASC&sort_by=1&etal=-1&type=title&starts_with=U
NIOSHTIC-2 Publications Search - 20027323 - Determination of benzo[a]pyrene-DNA adducts by solid-matrix phosphorescence.
It has been demonstrated for the first time that linear relationships can be obtained between the solid-matrix phosphorescence (SMP) and the percent modification of benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE)-DNA adducts. The samples of DNA were modified with BPDE at levels of 5.0 10-3, 1.0 10-3, 5.0 10-4, 1.0 10-4, 5.0 10-5, and 1.0 10-5%. In addition, the changes in the SMP inhttps://www.cdc.gov/niosh/nioshtic-2/20027323.html
A PBPK-model for B(a)P in the rat relating dose and liver DNA-adduct level - Webbased Archive of RIVM Publications
In M. Olling et al. (1995) an experiment was reported about the toxicokinetics of Benzo-a-Pyrene B(a)P in rats after repeated daily dosing p.o. From this experiment it appeared that the kinetic parameters AUC, Cmax and tmax varied as a function of time. At the first day of the experiment the values for AUC and Cmax were highest, while the value for tmax was lowest. At day 5 and day 12, i.e. the fifth day of the first and second week, AUC and Cmax were highest and tmax was lowest. However, at day 15, i.e. the first day after the weekend, during which the animals were not dosed, the values were intermediate and almost restored. The observations regarding Cmax can be explained by Ah-receptor dependent autoinduction of B(a)P. In Zeilmaker et al. (1997) a cellular model was reported for Ah-receptor dependent P450 induction. This model was incorporated in a Physiologically Based PharmacoKinetic (PBPK) model of the rat. In this report it is tried to explain the experimental observations of Olling et ...http://rivm.openrepository.com/rivm/handle/10029/10239
Blood/Cell DNA Maxi Kit (GDM010, GDM025) | Geneaid
The Genomic DNA Maxi Kit (Blood/Cultured Cell) is optimized for DNA purification (including genomic, mitochondrial and viral DNA) from up to 10 ml of frozen blood or fresh blood and up to 1 x 108 cultured cells using an efficient maxiprep system. This DNA Extraction Kit uses Protease to reduce hemoglobin contamination while chaotropihttp://www.geneaid.com/products/target-sample/blood/blood-dna-maxi-extraction-kit
In an article in the April 2000 issue of Cancer Epidemiology, Biomarkers & Prevention, an author's surname, "Besaratinia," appeared as "Nia." For consistency in the literature, the author would like to revise the author list for that article. The corrected citation appears below.. Besaratinia A, Maas LM, Van Breda SG, Curfs DM, Kleinjans JC, Wouters EF, Van Schooten FJ. Applicability of induced sputum for molecular dosimetry of exposure to inhalatory carcinogens: 32P-postlabeling of lipophilic DNA adducts in smokers and nonsmokers. Cancer Epidemiol Biomarkers Prev 2000;9:367-72.. ...http://cebp.aacrjournals.org/content/14/7/1846.2
Definition for exocyclic
exocyclic definition: exterior to a chemical band construction: an exocyclic double-bond.; (of a cyclic chemical) maybe not developing part of, or not connected to the ring; Pertaining to the Exocyclica;…https://www.azdictionary.com/definition/exocyclic
tex-live] tlpretest: gpg not found
Couldn't detect gpg so will proceed without verification! , gpg.exe is in C:\texlive\2016\tlpkg\installer\gpg, but is not in the Should be fixed after today, hopefully ;-) Sorry. Norbert ------------------------------------------------------------------------ PREINING, Norbert http://www.preining.info JAIST, Japan TeX Live & Debian Developer GPG: 0x860CDC13 fp: F7D8 A928 26E3 16A1 9FA0 ACF0 6CAC A448 860C DC13 ...https://tug.org/pipermail/tex-live/2016-April/038115.html
... ，男，博士，教授，公共卫生学院副院长，中国环境诱变剂学会毒性测试专业委员会常委，中华预防医学会卫生毒理学分会委员，中国毒理学会生殖毒理、临床毒理专委会委员。国家优秀青年基金、省杰出青年基金、教育部新世纪优秀人才、霍英东青年教师奖获得者、省333工程中青年科学技术带头人、省青蓝工程优秀青年骨干教师。2011年于美国加州大学劳伦斯伯克利国家实验室（LBNL）从事访问学者研究。获省部级科技进步奖一等奖1项，二等奖2项，主持国家基金4项，省部级课题4项，国际合作课题2项。发表SCI论著121篇，其中通讯作者38篇，第一作者11篇，包括Nat Genet、Nanoscale、Environ SciTechnol、Environ Int、Arch Toxicol等领域权威期刊，并被广泛引用。担任多份国际杂志编委和审稿人。获国家发明专利授权7件。 ...http://sklrm.njmu.edu.cn/1950/list.htm
From her very first lesson, Lena knew she would eventually become certified to teach this wonderful Technique. Over the years, AT has helped her improve her acting, movement and vocal abilities. Auditioning and performing has become a source of pleasure as opposed to a worry or fear. Creating in a room of her peers, which was once daunting and deadening, is now exciting and enlivening.. In addition to acting, Lena has experienced improvement in all areas of her life, including running, meditating, carrying heavy objects, etc…and has helped numerous people with their issues. Whether it be stage fright or back pain that is bothering you, Lena is eager to share what she has learned with the Technique to help solve your problems.. Lena is currently in her final term at the Alexander Education Center of Berkeley under Giora Pinkas, John Baron, and Robert Britton. Before she began her training in Berkeley, Lena studied the Alexander Technique for a total of 8 years under various teachers including ...http://lenahart.com/alexander-technique/