*  US Patent # 6,881,586. Retentate chromatography and protein chip arrays with applications in biology and medicine -...
This eluant can now be added to the set of constants, which now includes an anion exchange resin and a pH 6.5 buffer. In the ... 3) anion exchange, (4) cation exchange, and (5) immobilized metal (tris(carboxymethyl)ethylenediamine-Cu). Each adsorbent is ... 3) anion exchange (Q Sepharose, available from Sigma), (4) cation exchange (S Sepharose, available from Sigma), (5) immobilized ... or one may vary the density of the anion exchange resin. Again, a selectivity condition is identified from this set that shows ...
  http://patents.com/us-6881586.html
*  Patent US6451123 - Process for the separation of sugars - Google Patents
When a strong base anion exchange resin, such as a chloride-form strong base anion exchange resin, is conditioned with a low ... the conditioned resin separates a number of sugars and sugar alcohols from one another, while still allowing ready desorption ... from other sugars and sugar alcohols may be achieved by chromatography over hydroxyl-form anion exchange surfaces prepared from ... anion exchange resins at relatively low hydroxyl concentrations. ... borate buffers and borate forms of anion exchange resins have ...
  http://www.google.com.au/patents/US6451123
*  UNOsphere Anion Exchange Resin | Life Science Research | Bio-Rad
Use UNOsphere strong anion exchange resin for efficient biopharmaceutical molecule capture from crude feedstreams. Polymer with ... Desalting or buffer exchange. Bio-Scale Mini cartridges. Bio-Gel P-6. ... Home>Life Science Research>Products>Chromatography>Chromatography Resins>Anion Exchange Resin,UNOsphere Anion Exchange Resin ... UNOsphere Q support is used for strong anion exchange chromatography. UNOsphere ion exchange resins are designed to meet the ...
  http://www.bio-rad.com/en-ca/product/unosphere-anion-exchange-resin
*  715. Chymosin produced from Aspergillus niger var. awamori/calf prochymosin B gene (WHO Food Additives Series 28)
... absorption on a suitable anion-exchange resin followed by elution with a buffer containing 1 M sodium chloride (Pfizer Central ... Anion-Exchange Chromatography 5. Amino Acid Analysis 6. Amino Terminal Sequencing 7. Peptide Mapping 8. Composition and ... Subsequently, the solution containing prochymosin is diluted with a buffer, and the pH is reduced to 8.5- 9.5, followed by a 2- ... The harvested inclusion bodies are washed with phosphate buffer solution containing 1-4 M urea. The residual E. coli are ...
  http://www.inchem.org/documents/jecfa/jecmono/v28je08.htm
*  US5665239A - Processes for deionization and demineralization of fluids - Google Patents
... weakly basic anion exchange resin where an anion portion of said anion-buffer salt complex is split from said anion-buffer salt ... by adsorption of said anion portion onto said weakly basic anion exchange resin to form a second effluent comprising buffer in ... acidic cation exchange resin to occupy cation exchange sites and to form a first bed comprising a first amine-buffered resin; ( ... said first amine-buffered resin and exchanging cations of said fluid for amine cations of said first amine-buffered resin to ...
  https://patents.google.com/patent/US5665239?oq=5%2C583%2C822
*  A Highly Conserved Glycine within Linker I and the Extreme C Terminus of G Protein α Subunits Interact Cooperatively in...
The lysate was applied to AG 1-X8 anion exchange resin (Bio-Rad, Hercules, CA) and washed twice with buffer containing 60 mM ... Membrane protein (10 μg) was incubated in binding buffer (for composition, see Materials and Methods) with 0.1 nM 125I-MCH and ... Forty-eight hours after transfection, the cells were washed twice in HBSS buffer (including CaCl2 and MgCl2; catalog no. 14025- ... Competitor drug dilutions, radioligand, and cell membranes were all prepared in assay buffer [25 mM HEPES, pH 7.4, 10 mM MgCl2 ...
  http://jpet.aspetjournals.org/content/313/1/78.full
*  US5472864A - Method of preparing solution enriched in EG III using low molecular weight alcohol, organic salt and inorganic...
... was then loaded onto a column of 20 ml of QA Trisacryl M anion exchange resin equilibrated with 10 mM sodium phosphate buffer ... Louis, Mo.), QA Trisacryl M anion exchange resin and SP Trisacryl M cation exchange resin from IBF Biotechnics (Savage, Md.). ... The desalted solution, would then be loaded onto a QA Trisacryl M anion exchange resin column. The fraction not bound on this ... This solution, 200 ml, was then loaded onto a column of 20 ml of SP Trisacryl M cation exchange resin. The EG III was eluted ...
  https://patents.google.com/patent/US5472864
*  Performance of Three Resin Based Materials for Treating Uranium Contaminated Groundwater within a PRB. - White Rose Research...
These are a cation exchange resin, IRN 77; an anion exchange resin, Varion AP; and a recently developed material called PANSIL ... It operates by first acidifying the solution, then sorbing UO22+, and can release UO22+ when its buffering capacity has been ... The anion exchange resin is very effective at removing anionic uranyl carbonate species from solutions with a pH above 5, with ... In neutral groundwater containing carbonate, both the anion exchange resin and PANSIL exhibit conditional distribution ...
  http://eprints.whiterose.ac.uk/1328/
*  Compartmentation of sucrose during radial transfer in mature sorghum culm | BMC Plant Biology | Full Text
... in this buffer to completely convert fructose to a phosphate form for removal by strong anion exchange resin [14]. After this, ... The water content of the remaining resin slurry was again determined.. Water can become tritiated due to a solvent-exchange ... Tarpley L, Dahlberg JA, Vietor DM, Miller FR: Batch anion exchange separation and quantification of [14C]hexose from [14C] ... After 60 minutes at 37°C, 0.5 meq of the acetate form of Amberlite IRA-68 (Sigma), a weak anion exchanger, was added per mL. ...
  https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-7-33
*  Midiprep Plasmid Purification
... and the plasmid DNA binds to the anion-exchange resin. The first washing step (using equilibration buffer) is performed with ... Xtra purity-obtain the highest purity via proven anion exchange technology *Xtra flexible-choose from Midiprep/Maxiprep, with ... NucleoBond Xtra Midiprep kits are a new generation of anion exchange midiprep plasmid purification kits based on the patented ... Native Purification with TALON Resin Imidazole Elution Protocol-At-A-Glance * Native Purification with TALON Resin pH Elution ...
  http://www.clontech.com/US/Products/Nucleic_Acid_Purification/Plasmid/Midiprep/NucleoBond_Xtra_Midi?sitex=10020:22372:US&PROD=gTb3RVz80UH10imaw5WvPo8-:S&PROD_pses=ZG882F990E648F26DD8D27C71BDFF8123721A54F6BFCD84C38128752222283AEDF6C30CB08E9A29D3D3F4BD04588E30D9F59E8D9B95F68C3F1
*  Method for purifying antihemophilic factor - New York University
d) Anion exchange resins or hydrophobic affinity resins from any manufacturer may be used. Although some buffer adjustment may ... with Buffer II on an anion exchange column and washing with the same buffer permits AHF protein to bind ionically to the resin ... The column was then washed with 2 volumes of Buffer II, 5 volumes of Buffer III, 2 volumes of Buffer IV and 2 volumes of Buffer ... a) significantly enhances the affinity of AHF to an anion exchange resin selectively allowing its retention over a rather ...
  http://www.freepatentsonline.com/4847362.html
*  Sulfated Steroids as Natural Ligands of Mouse Pheromone-Sensing Neurons | Journal of Neuroscience
1 E, 95%), did not bind to a strong cation exchange resin (Fig. 1 F, 85%) but did bind to a strong anion exchange resin (Fig. 1 ... Fractions obtained from the ion exchange columns were buffered and then desalted using ODS SPE. WAX columns (weak anion ... AG1 resin (strong anion exchange) was from Bio-Rad; 5.25 g of resin was used for 21 ml equivalent of urine, with 1 m sodium ... strong anion exchange, 25 electrodes from 5 preparations; H , weak anion exchange, 138 electrodes from 6 preparations. I , ...
  http://www.jneurosci.org/content/28/25/6407.full
*  AppNote Purification of Amyloidogenic Transthyretin Protein | Future Lab
... we used high-capacity Nuvia Q Anion Exchange Resin to effectively purify it under neutral buffer conditions. ... Get More Information on Nuvia™ Q Anion Exchange Resin from Bio-Rad. ... Profinity™ IMAC Resin: An Optimal Support for His-Tagged Protein Purification Engineering Antibodies: A Fab-ulous Future for ... Here, we demonstrate how we were able to use Bio-Rad's Nuvia Q Resin to successfully purify one such protein, transthyretin ( ...
  http://futurelab.biocompare.com/4-production/article/appnote-purification-amyloidogenic-transthyretin-protein
*  DNA Extraction and Purification | Fisher Scientific
Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin. ... GE Healthcare Life Sciences™ llustra™ MicroSpin™ G-25 Columns are ideal for desalting, buffer exchange, and removal of ... Invitrogen™ PureLink™ Expi Endotoxin-Free Buffer Maxi Purify endotoxin-free plasmid DNA in half the time.The Invitrogen™ ... Invitrogen™ UltraPure™ 1M Tris-HCI Buffer, pH 8.0 Pre-mixed, pH-adjusted, sterile-filtered solutions ...
  https://www.fishersci.com/us/en/products/life-sciences/molecular-biologyreagentsandkits/nucleic-acid-purification/dna-extraction-andpurification.html
*  US5529989A - Pancratistatin prodrug - Google Patents
DEAE SEPHADEX A-25 weak anion exchange resin (acetate form) was washed (before using) first with aqueous 1M triethylammonium ... by anion-exchange chromatography using DEAE-SEPHADEX and gradient elution with an aqueous triethylammonium bicarbonate buffer ( ... After 10 minutes, the solution was diluted to 1L with water and placed on a DEAE-SEPHADEX weak anion exchange column (50 ml, ... The DOWEX 50X4-400 cation exchange resin was washed (prior to use) successively with aqueous 2N hydrochloric acid, distilled ...
  https://patents.google.com/patent/US5529989?oq=6016038
*  Spezifische Elektrolyteffekte in der Chromatographie von Biopharmazeutika
However, polyvalent anions adversely influence DNA removal. Effects of polyvalent ions regarding anion exchange chromatography ... Sodium phosphate buffer supports binding capacity of both resins for virus particles, which contradicts the behavior of ... particularly during anion exchange chromatography with a polyamine ligand. Capacity of the polyamine anion exchanger is up to ... Sodium phosphate buffer is beneficial for achieving virus recovery from the polyamine adsorber, compared to Tris/HCl buffer and ...
  http://tuprints.ulb.tu-darmstadt.de/5780/
*  Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting...
Elute through an AG-1 X8 100-200 anion exchange resin according to the manufacturer's instructions. ... Assay buffer solution: 100 mM Tris-HCl buffer (pH 7.5), 1.3 mM MnCl2, 0.33 mM EDTA, 0.1 mM ß-NADP+, 0.1 mM D-(+)-threo- ... Add sample buffer and boil lysates to prepare for loading.. *. Sample buffer: 0.5 mL 1 M TrisCl, pH 6.8, 1 mL glycerol, 0.5 mL ... anion exchange column. Reagent. Bio-Rad. 731-6211. Poly-Prep Columns, AG 1-X8, chloride form. ...
  https://elifesciences.org/articles/12626
*  Products in SPE Columns, Columns on Thomas Scientific
Exhibits both anion exchange and reversed phase behavior Wide pH range: can be ionized or neutral depending on pH of buffer ... The Agilent SampliQ Strong Anion Exchange (SAX) polymeric resin is a mixed-mode, tertiary amine-modified divinyl benzene ... SampliQ Silica Si-SAX (Strong Anion Exchange) SPE Cartridges Agilent Technologies. Ion Exchange Average particle size of 45 µm ... Weak Cation Exchange Exhibits both cation exchange and reversed phase behavior Wide pH range: can be ionized or neutral ...
  https://www.thomassci.com/nav/cat1/columns/cat2/columns_specolumns/0
*  Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent...
At the stage of anion-exchange chromatography, SPS is firmly bound to the DEAE-Sephadex A-50 anion-exchange carrier and ... change of the resin for Sephadex A-50 that allowed doing ion-exchange only once, without rechromatography, and differences in ... buffer components. The column with the carrier DEAE-Sephadex A-50 (parameters cm) was equilibrated with the same buffer used ... Figure 4: Anion-exchange chromatography on DEAE-Sephadex A-50 of SPS E197D preparation. -the base of the triangle shows the ...
  https://www.hindawi.com/journals/jaa/2013/983565/
*  US20020173453A1 - Method of treating renal injury - Google Patents
... an anion exchange process, such as using a quaternary amine resin (e.g. "Q-Sepharose," Pharmacia) and an eluant comprising 6M ... urea buffered with tris and 0.10M-0.16M NaCl, (v) a cation exchange process, such as using a sulfonic acid resin (e.g. "S- ... viii) subjecting the extracted protein to an anion exchange process; (ix) subjecting the extracted protein to a cation exchange ... The protein-containing urea solution is then subjected to sequential ion exchange chromatography, first anion exchange ...
  https://patents.google.com/patent/US20020173453?oq=821%2C393
*  Welcome to FABBX
The purified DNA is guanidine/anion exchange resin residues free.. This kit is designed for fast and efficient purification of ... Buffer A1 should be stored at 4°C once RNase A is added. All other materials can be stored at room temperature (22-25°C). The ... For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 2 times. Please contact our ... Unlike other kits in the markets, no chaotropic salts are contained in the buffer of our patented plasmid purification kit. ...
  http://fabbx.com/product.php?title=Plasmid%20Midi%20Kit%20II
*  The IPKat: August 2016
This patent claimed a purification of daptomycin using an anion exchange column (AEC) comprising an ion exchange resin, a ... the buffer, and urea, the chaotropic agent, are both standard agents employed in such purifications. Furthermore, there was no ... The invention utilises a reverse phase non-functional HP-20 SS resin, a standard resin in this field, in a method known as ... modified buffer, and a chaotropic agent, to prevent aggregation. Hospira alleged that the 179 patent lacked inventive step over ...
  http://ipkitten.blogspot.nl/2016_08_01_archive.html
*  Patent US20030185933 - Preparation of light stable hops - Google Patents
... from the ethanol extraction liquor obtained in the double extraction process by subjecting such liquor to an ion exchange ... Two BIO-RAD anion exchange resins (AG 1-X4, and 3-X4, 400 mesh, chloride form) were pre-rinsed with distilled water. The ... filtrate was diluted with a 1:1 ratio of either distilled water or a pH 8 buffer solution, and then added to the ion exchange ... the dissociated alpha/iso-alpha-acid ions exchange with these anions (Cl−, OH−, and acetate ion) on the resins. The ethanol ...
  http://www.google.co.in/patents/US20030185933
*  Unconventional secretion of Acb1 is mediated by autophagosomes
The medium was passed over an anion exchange resin that concentrates and purifies SDF-2, and the eluate was serially diluted ... The pellet was resuspended in 100 µl HU buffer (8 M urea, 5% SDS, 200 mM Tris, pH 6.8, and 1 mM EDTA with bromophenol blue as ... Samples were collected on exchange resin (A-25; Anion), washed with 100 mM NaCl, and eluted with 400 mM NaCl. The KP strain is ... GRASP is required for Acb1 secretion. (A) Wild-type and grh1Δ yeast were incubated in starvation buffer for 0, 1, 4, and 16 h, ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2828925/
*  Purification and Characterization of Gingipains - Current Protocols
DE‐52 anionexchange resin (Whatman). * Ion‐exchange buffer: 50 mM Bis‐Tris, pH 6.5/1 mM CaCl 2 ... 200 mM NaCl in ion‐exchange buffer for forming 0 to 200 mM linear gradient in gradient former ... A‐buffer (see recipe. ) supplemented with 200 µM Ac‐LVK‐ald (add from 10 mM Ac‐LVK‐ald stock, see recipe. ), for Rgp assay ... R‐buffer (see recipe. ) supplemented with 100 µM AcLVK‐ald (add from 10 mM Ac‐LVK‐ald stock, see recipe. ), for Rgp assay ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ps2120.html