CFR - Code of Federal Regulations Title 21
Anion and cation exchange resins buffered Benzethonium chloride Benzocaine Benzyl alcohol Bismuth subnitrate ...https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/cfrsearch.cfm?cfrpart=310&showfr=1
Anion and cation exchange resins buffered Benzethonium chloride Benzocaine Benzyl alcohol Bismuth subnitrate ...https://www.law.cornell.edu/cfr/text/21/310.545
US Patent # 5,478,805. Fractionation of polyalkylene oxide-conjugated hemoglobin solutions - Patents.com
... anion exchange resin, and buffers should be depyrogenated, utilizing standard procedures. Typically, the anion exchange resin ... 2. The method of claim 1 wherein said anion exchange medium comprises a quaternary amine coated anion exchange resin coated ... from the group consisting of quaternary amine coated anion exchange resins and polyethyleneimine coated anion exchange resins. ... recovering the fractions of conjugated hemoglobins not bound by the resin. Preferably, the anion exchange resin used in the ...http://patents.com/us-5478805.html
US Patent # 6,881,586. Retentate chromatography and protein chip arrays with applications in biology and medicine -...
This eluant can now be added to the set of constants, which now includes an anion exchange resin and a pH 6.5 buffer. In the ... 3) anion exchange, (4) cation exchange, and (5) immobilized metal (tris(carboxymethyl)ethylenediamine-Cu). Each adsorbent is ... 3) anion exchange (Q Sepharose, available from Sigma), (4) cation exchange (S Sepharose, available from Sigma), (5) immobilized ... or one may vary the density of the anion exchange resin. Again, a selectivity condition is identified from this set that shows ...http://patents.com/us-6881586.html
US Patent # 5,264,555. Process for hemoglobin extraction and purification - Patents.com
Methods are disclosed for separating hemoglobin from erythrocytes by contacting erythrocytes with a hypotonic buffer solution ... The chromatography equipment, anion exchange resin, equilibration buffer and elution buffer should be depyrogenated utilizing ... The anion exchange process preferably chromatographically treats hemoglobin solutions with an anion exchange resin in a liquid ... The method of claim 28, wherein said anion exchange medium comprises an anion exchange chromatography resin, and said step of ...http://patents.com/us-5264555.html
Patent US6451123 - Process for the separation of sugars - Google Patents
When a strong base anion exchange resin, such as a chloride-form strong base anion exchange resin, is conditioned with a low ... the conditioned resin separates a number of sugars and sugar alcohols from one another, while still allowing ready desorption ... from other sugars and sugar alcohols may be achieved by chromatography over hydroxyl-form anion exchange surfaces prepared from ... anion exchange resins at relatively low hydroxyl concentrations. ... borate buffers and borate forms of anion exchange resins have ...http://www.google.com.au/patents/US6451123
UNOsphere Anion Exchange Resin | Life Science Research | Bio-Rad
Use UNOsphere strong anion exchange resin for efficient biopharmaceutical molecule capture from crude feedstreams. Polymer with ... Desalting or buffer exchange. Bio-Scale Mini cartridges. Bio-Gel P-6. ... Home>Life Science Research>Products>Chromatography>Chromatography Resins>Anion Exchange Resin,UNOsphere Anion Exchange Resin ... UNOsphere Q support is used for strong anion exchange chromatography. UNOsphere ion exchange resins are designed to meet the ...http://www.bio-rad.com/en-ca/product/unosphere-anion-exchange-resin
715. Chymosin produced from Aspergillus niger var. awamori/calf prochymosin B gene (WHO Food Additives Series 28)
... absorption on a suitable anion-exchange resin followed by elution with a buffer containing 1 M sodium chloride (Pfizer Central ... Anion-Exchange Chromatography 5. Amino Acid Analysis 6. Amino Terminal Sequencing 7. Peptide Mapping 8. Composition and ... Subsequently, the solution containing prochymosin is diluted with a buffer, and the pH is reduced to 8.5- 9.5, followed by a 2- ... The harvested inclusion bodies are washed with phosphate buffer solution containing 1-4 M urea. The residual E. coli are ...http://www.inchem.org/documents/jecfa/jecmono/v28je08.htm
US5665239A - Processes for deionization and demineralization of fluids - Google Patents
... weakly basic anion exchange resin where an anion portion of said anion-buffer salt complex is split from said anion-buffer salt ... by adsorption of said anion portion onto said weakly basic anion exchange resin to form a second effluent comprising buffer in ... acidic cation exchange resin to occupy cation exchange sites and to form a first bed comprising a first amine-buffered resin; ( ... said first amine-buffered resin and exchanging cations of said fluid for amine cations of said first amine-buffered resin to ...https://patents.google.com/patent/US5665239?oq=5%2C583%2C822
A Highly Conserved Glycine within Linker I and the Extreme C Terminus of G Protein α Subunits Interact Cooperatively in...
The lysate was applied to AG 1-X8 anion exchange resin (Bio-Rad, Hercules, CA) and washed twice with buffer containing 60 mM ... Membrane protein (10 μg) was incubated in binding buffer (for composition, see Materials and Methods) with 0.1 nM 125I-MCH and ... Forty-eight hours after transfection, the cells were washed twice in HBSS buffer (including CaCl2 and MgCl2; catalog no. 14025- ... Competitor drug dilutions, radioligand, and cell membranes were all prepared in assay buffer [25 mM HEPES, pH 7.4, 10 mM MgCl2 ...http://jpet.aspetjournals.org/content/313/1/78.full
US5472864A - Method of preparing solution enriched in EG III using low molecular weight alcohol, organic salt and inorganic...
... was then loaded onto a column of 20 ml of QA Trisacryl M anion exchange resin equilibrated with 10 mM sodium phosphate buffer ... Louis, Mo.), QA Trisacryl M anion exchange resin and SP Trisacryl M cation exchange resin from IBF Biotechnics (Savage, Md.). ... The desalted solution, would then be loaded onto a QA Trisacryl M anion exchange resin column. The fraction not bound on this ... This solution, 200 ml, was then loaded onto a column of 20 ml of SP Trisacryl M cation exchange resin. The EG III was eluted ...https://patents.google.com/patent/US5472864
AnionExchange problem: proteins do not bind - Protein Expression and Purification - BioForum
Anion/Cation EX; Gelfiltration). I m having tremendous problems with the first step: an anionexchange column (1ml Q XL from GE ... AnionExchange problem: proteins do not bind - posted in Protein Expression and Purification: Hi folks, I m an absolute beginner ... Buffer Tris-HCl (10 mM) can help...then, u dialyze/ buffer exchange ur sample to this buffer.... Secondly, by rule of thumb.... ... since u use Q resin....i do believe it is positively change resin.if u want to make ur protein bind to the resin, u should make ...http://www.protocol-online.org/forums/topic/15090-anionexchange-problem-proteins-do-not-bind/
Performance of Three Resin Based Materials for Treating Uranium Contaminated Groundwater within a PRB. - White Rose Research...
These are a cation exchange resin, IRN 77; an anion exchange resin, Varion AP; and a recently developed material called PANSIL ... It operates by first acidifying the solution, then sorbing UO22+, and can release UO22+ when its buffering capacity has been ... The anion exchange resin is very effective at removing anionic uranyl carbonate species from solutions with a pH above 5, with ... In neutral groundwater containing carbonate, both the anion exchange resin and PANSIL exhibit conditional distribution ...http://eprints.whiterose.ac.uk/1328/
Compartmentation of sucrose during radial transfer in mature sorghum culm | BMC Plant Biology | Full Text
... in this buffer to completely convert fructose to a phosphate form for removal by strong anion exchange resin . After this, ... The water content of the remaining resin slurry was again determined.. Water can become tritiated due to a solvent-exchange ... Tarpley L, Dahlberg JA, Vietor DM, Miller FR: Batch anion exchange separation and quantification of [14C]hexose from [14C] ... After 60 minutes at 37°C, 0.5 meq of the acetate form of Amberlite IRA-68 (Sigma), a weak anion exchanger, was added per mL. ...https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-7-33
Midiprep Plasmid Purification
... and the plasmid DNA binds to the anion-exchange resin. The first washing step (using equilibration buffer) is performed with ... Xtra purity-obtain the highest purity via proven anion exchange technology *Xtra flexible-choose from Midiprep/Maxiprep, with ... NucleoBond Xtra Midiprep kits are a new generation of anion exchange midiprep plasmid purification kits based on the patented ... Native Purification with TALON Resin Imidazole Elution Protocol-At-A-Glance * Native Purification with TALON Resin pH Elution ...http://www.clontech.com/US/Products/Nucleic_Acid_Purification/Plasmid/Midiprep/NucleoBond_Xtra_Midi?sitex=10020:22372:US&PROD=gTb3RVz80UH10imaw5WvPo8-:S&PROD_pses=ZG882F990E648F26DD8D27C71BDFF8123721A54F6BFCD84C38128752222283AEDF6C30CB08E9A29D3D3F4BD04588E30D9F59E8D9B95F68C3F1
Method for purifying antihemophilic factor - New York University
d) Anion exchange resins or hydrophobic affinity resins from any manufacturer may be used. Although some buffer adjustment may ... with Buffer II on an anion exchange column and washing with the same buffer permits AHF protein to bind ionically to the resin ... The column was then washed with 2 volumes of Buffer II, 5 volumes of Buffer III, 2 volumes of Buffer IV and 2 volumes of Buffer ... a) significantly enhances the affinity of AHF to an anion exchange resin selectively allowing its retention over a rather ...http://www.freepatentsonline.com/4847362.html
Sulfated Steroids as Natural Ligands of Mouse Pheromone-Sensing Neurons | Journal of Neuroscience
1 E, 95%), did not bind to a strong cation exchange resin (Fig. 1 F, 85%) but did bind to a strong anion exchange resin (Fig. 1 ... Fractions obtained from the ion exchange columns were buffered and then desalted using ODS SPE. WAX columns (weak anion ... AG1 resin (strong anion exchange) was from Bio-Rad; 5.25 g of resin was used for 21 ml equivalent of urine, with 1 m sodium ... strong anion exchange, 25 electrodes from 5 preparations; H , weak anion exchange, 138 electrodes from 6 preparations. I , ...http://www.jneurosci.org/content/28/25/6407.full
1 E, 95%), did not bind to a strong cation exchange resin (Fig. 1 F, 85%) but did bind to a strong anion exchange resin (Fig. 1 ... Fractions obtained from the ion exchange columns were buffered and then desalted using ODS SPE. WAX columns (weak anion ... AG1 resin (strong anion exchange) was from Bio-Rad; 5.25 g of resin was used for 21 ml equivalent of urine, with 1 m sodium ... strong anion exchange, 25 electrodes from 5 preparations; H , weak anion exchange, 138 electrodes from 6 preparations. I , ...http://www.jneurosci.org/content/28/25/6407
AppNote Purification of Amyloidogenic Transthyretin Protein | Future Lab
... we used high-capacity Nuvia Q Anion Exchange Resin to effectively purify it under neutral buffer conditions. ... Get More Information on Nuvia™ Q Anion Exchange Resin from Bio-Rad. ... Profinity™ IMAC Resin: An Optimal Support for His-Tagged Protein Purification Engineering Antibodies: A Fab-ulous Future for ... Here, we demonstrate how we were able to use Bio-Rad's Nuvia Q Resin to successfully purify one such protein, transthyretin ( ...http://futurelab.biocompare.com/4-production/article/appnote-purification-amyloidogenic-transthyretin-protein
US5529989A - Pancratistatin prodrug - Google Patents
DEAE SEPHADEX A-25 weak anion exchange resin (acetate form) was washed (before using) first with aqueous 1M triethylammonium ... by anion-exchange chromatography using DEAE-SEPHADEX and gradient elution with an aqueous triethylammonium bicarbonate buffer ( ... After 10 minutes, the solution was diluted to 1L with water and placed on a DEAE-SEPHADEX weak anion exchange column (50 ml, ... The DOWEX 50X4-400 cation exchange resin was washed (prior to use) successively with aqueous 2N hydrochloric acid, distilled ...https://patents.google.com/patent/US5529989?oq=6016038
DNA Extraction and Purification | Fisher Scientific
Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin. ... GE Healthcare Life Sciences™ llustra™ MicroSpin™ G-25 Columns are ideal for desalting, buffer exchange, and removal of ... Invitrogen™ PureLink™ Expi Endotoxin-Free Buffer Maxi Purify endotoxin-free plasmid DNA in half the time.The Invitrogen™ ... Invitrogen™ UltraPure™ 1M Tris-HCI Buffer, pH 8.0 Pre-mixed, pH-adjusted, sterile-filtered solutions ...https://www.fishersci.com/us/en/products/life-sciences/molecular-biologyreagentsandkits/nucleic-acid-purification/dna-extraction-andpurification.html
Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting...
Elute through an AG-1 X8 100-200 anion exchange resin according to the manufacturer's instructions. ... Assay buffer solution: 100 mM Tris-HCl buffer (pH 7.5), 1.3 mM MnCl2, 0.33 mM EDTA, 0.1 mM ß-NADP+, 0.1 mM D-(+)-threo- ... Add sample buffer and boil lysates to prepare for loading.. *. Sample buffer: 0.5 mL 1 M TrisCl, pH 6.8, 1 mL glycerol, 0.5 mL ... anion exchange column. Reagent. Bio-Rad. 731-6211. Poly-Prep Columns, AG 1-X8, chloride form. ...https://elifesciences.org/articles/12626
Spezifische Elektrolyteffekte in der Chromatographie von Biopharmazeutika
However, polyvalent anions adversely influence DNA removal. Effects of polyvalent ions regarding anion exchange chromatography ... Sodium phosphate buffer supports binding capacity of both resins for virus particles, which contradicts the behavior of ... particularly during anion exchange chromatography with a polyamine ligand. Capacity of the polyamine anion exchanger is up to ... Sodium phosphate buffer is beneficial for achieving virus recovery from the polyamine adsorber, compared to Tris/HCl buffer and ...http://tuprints.ulb.tu-darmstadt.de/5780/
Products in SPE Columns, Columns on Thomas Scientific
Exhibits both anion exchange and reversed phase behavior Wide pH range: can be ionized or neutral depending on pH of buffer ... The Agilent SampliQ Strong Anion Exchange (SAX) polymeric resin is a mixed-mode, tertiary amine-modified divinyl benzene ... SampliQ Silica Si-SAX (Strong Anion Exchange) SPE Cartridges Agilent Technologies. Ion Exchange Average particle size of 45 µm ... Weak Cation Exchange Exhibits both cation exchange and reversed phase behavior Wide pH range: can be ionized or neutral ...https://www.thomassci.com/nav/cat1/columns/cat2/columns_specolumns/0
Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent...
At the stage of anion-exchange chromatography, SPS is firmly bound to the DEAE-Sephadex A-50 anion-exchange carrier and ... change of the resin for Sephadex A-50 that allowed doing ion-exchange only once, without rechromatography, and differences in ... buffer components. The column with the carrier DEAE-Sephadex A-50 (parameters cm) was equilibrated with the same buffer used ... Figure 4: Anion-exchange chromatography on DEAE-Sephadex A-50 of SPS E197D preparation. -the base of the triangle shows the ...https://www.hindawi.com/journals/jaa/2013/983565/