*  Development and Functional Characterization of Murine Tolerogenic Dendritic Cells | Protocol
... we present a protocol to develop and characterize tolerogenic dendritic cells (TolDCs) and evaluate their immunotherapeutic... ... in vitro from bone marrow progenitor cells or peripheral blood monocytes using granulocyte-macrophage-colony-stimulating factor ... Bone marrow progenitor cells were cultured in complete RPMI medium in the presence of GM-CSF and IL-4 to differentiate into ... NOTE: The bone marrow cells from one mouse is around 3 - 5 x 107 cells, indicating that it can be placed from 2 to 3 6-well ...
  https://www.jove.com/video/57637/development-functional-characterization-murine-tolerogenic-dendritic
*  Demonstration of early functional compromise of bone marrow derived hematopoietic progenitor cells during bovine neonatal...
105 cells per ml. Granulocyte/macrophage colony-stimulating factor (rboGM-CSF expressed in CHO cells using a previously defined ... bone marrow derived hematopoietic progenitor cells during bovine neonatal pancytopenia through in vitro culture of bone marrow ... We describe here a pilot study on the utility of bone marrow hematopoietic progenitor cell (BM-HPC) cultures to assess the ... Muiya P, Logan-Henfrey L, Naessens J: Expression of antigens on haemopoietic progenitor cells in bovine bone marrow. Vet ...
  https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-5-599
*  The TH1 Lymphokine Interferon-γ is a Potent Upregulator of Dendritic Cells with Phagocytic Capacity in GM-CSF Supplemented Bone...
... macrophages and granulocytes are descendants of a hematopoietic progenitor cell that originates in the bone marrow1. Thus, bone ... Dendritic cells from mouse bone marrow: in vitro differentiation using low doses of recombinant granulocyte-macrophage colony- ... macrophages and granulocytes are descendants of a hematopoietic progenitor cell that originates in the bone marrow1. Thus, bone ... Dendritic Cell Cutaneous Leishmaniasis Mouse Bone Marrow Bone Marrow Culture Phagocytic Capacity These keywords were added by ...
  https://rd.springer.com/chapter/10.1007/978-1-4757-9966-8_37
*  JCI - The stem cell niches in bone
... cell development. CMP, common myeloid progenitor; CLP, common lymphoid progenitor; GMP, granulocyte/macrophage progenitor; MKEP ... Human osteoblasts support human hematopoietic progenitor cells in vitro bone marrow cultures. Blood. 87:518-524. View this ... Hematopoiesis of bone cells and marrow stromal cells. In the bone marrow HSCs reside either next to osteoblasts on the ... Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Cell. 109: ...
  https://www.jci.org/articles/view/JCI28568
*  US5470569A - Recombinant colony stimulating factor-1 - Google Patents
... variety of tissues to stimulate the growth and development of bone marrow progenitor cells into granulocytes and/or macrophages ... which macrophages are produced by in vitro culture of bone marrow or other suitable preparations treated with CSF-1. These ... For the bone marrow stimulation assay, which measures biological activity of the protein, bone marrow cells from Balb/C mice ... In addition, a murine factor called IL-3 induces colonies from murine bone marrow cells which contain all these cell types plus ...
  https://patents.google.com/patent/US5470569
*  Cordyceps sinensis health supplement enhances recovery from taxol-induced leukopenia. - PubMed - NCBI
It appears to do so by protecting both hematopoietic progenitor cells directly and the bone marrow stem cell niche through its ... In vitro assays showed that CS enhanced the colony-forming ability of both granulocyte macrophage colony forming unit (GM-CFU) ... and osteogenic cells from bone marrow preparations and promoted the differentiation of bone marrow mesenchymal stromal cells ... CS effects on BM cells in vitro. (A) CS enhanced the survival of GM-CFUs from BM cells. BM cells were harvested from the tibia ...
  https://www.ncbi.nlm.nih.gov/pubmed/18367634
*  In Vivo 4-1BB Deficiency in Myeloid Cells Enhances Peripheral T Cell Proliferation by Increasing IL-15 | The Journal of...
... and granulocyte-macrophage progenitors and that 4-1BBL signaling inhibited the differentiation of myeloid progenitors into DC ... bone marrow. CBA. cytometric bead array. CD62L. L-selectin. Cl2MDP. clodronate. DC. dendritic cell. InLN. inguinal lymph node. ... Because in vitro 4-1BB signaling of activated T cells routinely enhances the multiplication of T cells and stimulates their ... Alternatively, cytotoxic CD8+ T cells may suppress NK cell expansion as NK cells are known to do the same for CD8+ T cells (39 ...
  http://www.jimmunol.org/content/194/4/1580
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... we performed bone marrow transplantation of transduced Mpl(-/-) bone marrow cells into Mpl(-/-) mice. We show that restoration ... Ectopic HOXC6 expression also induced strong myeloid differentiation and expansion of granulocyte-macrophage progenitors/common ... We have identified HOXC6 as a factor that enhances competitive repopulation capacity in vivo and colony formation in vitro. ... accompanied by bone neo-formation and marrow fibrosis. Gene-corrected Mpl(-/-) cells had increased long-term repopulating ...
  https://www.jove.com/visualize?author=Dirk+Heckl
*  Hematopoiesis from Pluripotent Stem Cells | Thermo Fisher Scientific - DE
Explore hematopoiesis from pluripotent stem cells and find antibodies to detect some of its target proteins, including IL-3, GM ... Basophils arise from CD34+ progenitor cells found in cord blood, peripheral blood, and bone marrow. Basophilic differentiation ... stem cell factor), GMCSF (granulocyte-macrophage colony-stimulating factor), IL-1 (interleukin-1), IL-6 (interleukin-6), IL-11 ... Several of the CSFs stimulate colony formation of progenitors in vitro and have a broad spectrum of overlapping activities. ...
  http://www.thermofisher.com/de/de/home/life-science/antibodies/antibodies-learning-center/antibodies-resource-library/cell-signaling-pathways/hematopoiesis-pluripotent-stem-cells.html
*  Growth Factors in the Treatment of HIV Disease | SpringerLink
Steinberg, H. N., Crumpacker, C. S., and Chatis, P. A., 1991, In vitro suppression of normal human bone marrow progenitor cells ... Effect of granulocyte-macrophage colony-stimulating factor on rat alveolar macrophage anticryptococcal activity in vitro, J. ... Absent or rare HIV infection of bone marrow stem/progenitor cells in vivo, J. Virol. 65:1985-1990.PubMedGoogle Scholar ... Macrophage-active colony-stimulating factors enhance human immunodeficiency virus type 1 infection in bone marrow stem cells, ...
  https://link.springer.com/chapter/10.1007/978-1-4899-0191-0_27
*  Ex Vivo Expansion of Stem and Progenitor Cells Using Thrombopoietin | SpringerLink
... granulocyte-macrophage, and megakaryocytic progenitor cells in normal and myelosuppressed mice. Exp Hematol 24:265-269PubMed ... Incubation of murine bone marrow cells in hypoxia ensures the maintenance of marrow-repopulating ability together with the ... hematopoietic cells: in vitro and in vivo confirmation of neutropenia abrogation related to the expansion process without ... In: Hayat M. (eds) Stem Cells and Cancer Stem Cells, Volume 8. Stem Cells and Cancer Stem Cells, vol 8. Springer, Dordrecht. * ...
  https://link.springer.com/chapter/10.1007/978-94-007-4798-2_33
*  US5945337A - Method for culturing CD34+ cells in a serum-free medium - Google Patents
... cells purified from normal bone marrow, peripheral blood of patients treated with cytokines, and umbilical cord blood is ... used to stimulate proliferation and differentiation of mid-progenitor cells are interleukin-3 and granulocyte-macrophage colony ... cells that are tainted with tumor cells, either of bone marrow or metastatic origin, are placed into in vitro culture in the ... cultivating a mixed human bone marrow cell population, containing CD34+ cells and non-CD34+ cells in the population, in a serum ...
  https://patents.google.com/patent/US5945337
*  PI3K-dependent ERK is involved in GM-CSF-mediated activation of progenitor cells in a neovascularisation model
Palabras clave : co-culture model; endothelial progenitor cell; extracellular signal-regulated kinase; granulocyte-macrophage ... on rat endothelial progenitor cell (EPC) biology. EPCs were isolated from rat bone marrow and experiments were carried out to ... to determine the effect of GM-CSF on EPC angiogenesis in vitro. EPC proliferation and viability, migration, and vasculogenesis ... ZHAO, Wei y YANG, Xiang-Hong. PI3K-dependent ERK is involved in GM-CSF-mediated activation of progenitor cells in a ...
  http://www.scielo.org.za/scielo.php?script=sci_abstract&pid=S0038-23532010000300016&lng=es&nrm=iso&tlng=en
*  Stanley Schrier | Stanford Medicine Profiles
We identify a recurrent loss of granulocyte-macrophage progenitors (GMPs) in the bone marrow of low risk MDS patients that can ... Human bone marrow hematopoietic stem cells are increased in frequency and myeloid-biased with age PROCEEDINGS OF THE NATIONAL ... Blocking calreticulin on low risk MDS myeloid progenitors rescues them from phagocytosis in vitro. However, in the high-risk ... Evaluation of bone marrow plasma cell infiltration and presence of myeloma associated end organ damage could be clinically ...
  https://med.stanford.edu/profiles/stanley-schrier
*  Steven Goodman | Stanford Medicine Profiles
Bone marrow granulocyte and macrophage colony-forming units (CFU-GM) were assayed in vitro for dose response to 4- ... Pharmacodynamic analysis of individual patient bone marrow progenitor cell sensitivity did not reveal any correlation with age ... Severe aplastic anemia (SAA) is a life-threatening bone marrow failure disorder that can be treated with bone marrow ... EFFICACY OF IMMUNE GLOBULIN IN PREVENTING COMPLICATIONS OF BONE-MARROW TRANSPLANTATION - A METAANALYSIS BONE MARROW ...
  https://med.stanford.edu/profiles/steven-goodman
*  The FLT3 ligand is a direct and potent stimulator of the growth of primitive and committed human CD34+ bone marrow progenitor...
... granulocyte colony-stimulating factor (G-CSF), CSF-1, granulocyte macrophage (GM) CSF stem cell factor (SCF), and IL-6. FL and ... progenitor cells. FL alone was a weak growth stimulator of CD34+ BM cells, but synergistically and directly enhanced colony ... CD71+ BM cells. Thus, FL potently stimulates the growth of primitive and more committed human BM progenitor cells. ... subset of the CD34+ progenitors, as 4.5-fold more colonies were formed by CD34+CD71- cells in response to FL + IL-3 + SCF than ...
  https://www.rdm.ox.ac.uk/publications/573533
*  Cancer Stem Cells-Perspectives on Current Status and Future Directions: AACR Workshop on Cancer Stem Cells | Cancer Research
... bone marrow samples is encouraging in that patients in blast crisis all exhibited an expansion of the granulocyte-macrophage ... In addition, it is thought that stem cells may divide symmetrically to form two progenitor cells, which could lead to stem cell ... Several in vitro assays have been used to identify stem cells, including sphere assays, serial colony-forming unit (CFU) assays ... or an asymmetrical self-renewing cell division, resulting in one stem cell and one more differentiated progenitor cell. ...
  http://cancerres.aacrjournals.org/content/66/19/9339
*  CFU-DL - Wikipedia
... other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow ... Reid CD, Stackpoole A, Meager A, Tikerpae J (October 1992). "Interactions of tumor necrosis factor with granulocyte-macrophage ... CFU-DL is a colony forming unit that gives rise to Langerhans cells. ...
  https://en.wikipedia.org/wiki/CFU-DL
*  SIRT1 DEFICIENCY COMPROMISES MOUSE EMBRYONIC STEM CELL DIFFERENTIATION, AND EMBRYONIC AND ADULT HEMATOPOIESIS IN THE MOUSE
Moreover, knockout of SIRT1 decreased primary bone marrow hematopoietic progenitor cells (HPCs) in 5 week and 12 month old mice ... there were also significant decreases in granulocyte-macrophage (CFU-GM) and multipotential (CFU-GEMM) progenitor cells. ... cells in vitro, and hematopoietic progenitors in SIRT1+/+, SIRT1+/-, and SIRT1-/- mice. SIRT1-/- ES cells exhibited markedly ... Reintroduction of WT SIRT1 into SIRT1-/- cells partially rescued the primitive erythroid progenitor formation of SIRT1-/- cells ...
  https://scholarworks.iupui.edu/handle/1805/2523
*  IL-33 Precedes IL-5 in Regulating Eosinophil Commitment and Is Required for Eosinophil Homeostasis | The Journal of Immunology
... eosinophils in vitro in which bone marrow cells were cultured in SCF and Flt3L for the first 4 d to expand the progenitor cells ... granulocyte-macrophage progenitor. ILC2. type 2 innate lymphoid cell. KO. knockout. SCF. stem cell factor. WT. wild-type.. ... Bone marrow cells were counted, and 5 × 106 cells were used for staining. Cells were washed with PBS and stained with 0.25 μl ... Lineage(-)Sca1+c-Kit(-)CD25+ cells are IL-33-responsive type 2 innate cells in the mouse bone marrow. J. Immunol. 187: 5795- ...
  http://www.jimmunol.org/content/197/9/3445
*  GPR84 - Wikipedia
... in the bone marrow) that arise from dysregulated production of myeloid progenitor cells in the bone marrow. Ly6G + MDSCs in Lal ... GPR84 is normally expressed at low levels in myeloid cells and can be induced in vitro by stimulating macrophage or microglial ... GPR84 was also upregulated on both macrophages and neutrophyl granulocytes after LPS stimulation. Not only LPS challenge but ... There was also a GPR84 downregulation in dentritic cell derived from FcRgamma chain KO mice. In microglial cells, the GPR84 ...
  https://en.wikipedia.org/wiki/GPR84
*  Modeling Bone Marrow Progenitor Cell Differentiation and Susceptibility to HIV-1 Infection | MedCrave
... and recombinant human granulocyte-macrophage-colony stimulating factor (rhGM-CSF, 2 ng/ml; eBioscience, San Diego, CA). The ... Steinberg HN, Crumpacker CS, Chatis PA (1991) In vitro suppression of normal human bone marrow progenitor cells by human ... has strongly suggested that these cells are differentiating towards macrophages.. TF-1 cells, similar to bone marrow progenitor ... ancestral hematopoietic progenitor cells, as well as more mature hematopoietic progenitors and bone marrow progenitor stromal ...
  http://googlescholar.medcraveonline.com/scholars/article_fulltext/195
*  BCR-ABL Transcript Dynamics Support the Hypothesis That Leukemic Stem Cells Are Reduced during Imatinib Treatment | Clinical...
CMP, common myeloid progenitor; GMP, granulocyte macrophage progenitor; LT-HSC, long-term hematopoietic stem cell; MPP, ... This article combines mathematical modeling with recent clinical observations of progenitor cell kinetics in the bone marrow to ... Philadelphia-positive stem cells from patients with chronic myeloid leukemia are insensitive to STI571 in vitro . Blood 2002;99 ... evaluated the dynamics of several different subpopulations of progenitor cells (granulocyte-macrophage progenitors ...
  http://clincancerres.aacrjournals.org/content/17/21/6812
*  Articles Added on January 26, 2013
Although bone marrow (BM) inflammatory cells are the main source of eosinophil-basophil (Eo/B) differentiation-inducing ... Toll-like receptor-mediated eosinophil-basophil differentiation: autocrine signalling by granulocyte-macrophage colony- ... Cigarette smoke increases BLT2 receptor functions in bronchial epithelial cells: in vitro ... ... mediated signalling in BM progenitors. Having previously demonstrated ... ...
  http://www.biomedsearch.com/added/20130126.html
*  Hematopoietic Stem and Progenitor Cells Can Be Enriched by Implanting Biomaterial into Spatium Intermusculare
Figure 4: Hematopoietic stem and progenitor cells (HSPCs) in the migrating cells (MCs) originated in the bone marrow (BM). (a) ... colony-forming-unit-granulocyte, macrophage: GFU-GM, and colony-forming-unit-granulocyte, erythroid, macrophage, megakaryocyte ... In Vitro CFU Assay. MCs and BM cells were suspended in 2% FBS containing DMEM and then mixed with methylcellulose-based ... H. Kawada and M. Ogawa, "Bone marrow origin of hematopoietic progenitors and stem cells in murine muscle," Blood, vol. 98, no. ...
  https://www.hindawi.com/journals/bmri/2015/398642/