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*  Nobel goes to former Princeton researcher for discovery made here
... the GFP's chromophore simply transforms the blue light from aequorin into green light. This is why the jellyfish and aequorin ... yellowish in the light from a light bulb and fluorescent green in ultraviolet light. They called it the green protein, but it ... Strangely enough, the flash of light was not green like the edges of the jellyfish. It was blue.. Johnson and Shimomura ... The protein does not need any additives to glow -- instead it must be radiated with ultraviolet or blue light before turning ...
  https://www.princeton.edu/news/2008/10/08/nobel-goes-former-princeton-researcher-discovery-made-here?from=2010-01-01&to=2010-02-01
*  Luciferin - Wikipedia
It is the prosthetic group in the protein aequorin responsible for the blue light emission. Dinoflagellate luciferin is a ... Like the compound coelenterazine, it is an imidazopyrazinone and emits primarily blue light in the animals. While there may be ... Luciferin (from the Latin lucifer, "light-bringer") is a generic term for the light-emitting compound found in organisms that ... It is the substrate of beetle luciferases (EC 1.13.12.7) responsible for the characteristic yellow light emission from ...
  https://en.wikipedia.org/wiki/Luciferin
*  What are Fluorescent Proteins?
Aequorin emits blue light in a calcium dependent manner, while GFP emits green light when irradiated with light of 488 nm, ... Blue and Cyan Fluorescent Proteins. Blue fluorescent protein was generated by mutating a tyrosine residue to histidine present ... Blue and Cyan Fluorescent Proteins. Yellow Fluorescent Proteins. Yellow fluorescent protein (YFP) is a mutant variant of GFP. ...
  https://www.news-medical.net/life-sciences/What-are-Fluorescent-Proteins.aspx
*  2014 January
These Arabidopsis plants contain aequorin. Aequorin produces blue light as a result of oxidation of the substrate, in a calcium ... Increased calcium were bound to aequorin, which leads to blue light emission. Courtesy Jeongmin Choi ... "If you add ATP to wild-type plants, calcium concentrations go up and the plants produce more blue light," Choi said. "We found ... the two-year process boiled down to whether a plant would produce light when ATP was added. Since aequorin only luminesces when ...
  https://decodingscience.missouri.edu/2014/01/
*  Bioluminescence - Wikipedia
Furthermore, some of the blue light released by aequorin in contact with calcium ions is absorbed by a green fluorescent ... Most marine light-emission is in the blue and green light spectrum. However, some loose-jawed fish emit red and infrared light ... The blue-green light is emitted through the translucent shell, which functions as an efficient diffuser of light. Communication ... At rest it emits a dull glow but when disturbed it darts away leaving a cloud of shimmering blue light to confuse the predator ...
  https://en.wikipedia.org/wiki/Bioluminescence
*  Aequorin - Wikipedia
... while aequorin by itself generates blue light. Discussions of "jellyfish DNA" to make "glowing" animals often refer to ... which changes the native blue light of aequorin to green. While the main focus of his work was on the bioluminescence, ... Since the emitted light can be easily detected with a luminometer, aequorin has become a useful tool in molecular biology for ... Inouye S (2004). "Blue fluorescent protein from the calcium-sensitive photoprotein aequorin is a heat-resistant enzyme, ...
  https://en.wikipedia.org/wiki/Aequorin
*  Chemiluminescence - Wikipedia
Another protein, aequorin, found in certain jellyfish, produces blue light in the presence of calcium. It can be used in ... The iron from the blood acts as a catalyst and reacts with the luminol and hydrogen peroxide to produce blue light for about 30 ... The common colors for the light emitted by these animals are blue and green because they have shorter wavelength than red and ... A light stick emits light by chemiluminescence. Luminol in an alkaline solution with hydrogen peroxide in the presence of iron ...
  https://en.wikipedia.org/wiki/Chemiluminescence
*  John Woodland Hastings - Wikipedia
... aequorin), which alone emits blue light, to a secondary green emitter which they termed green fluorescent protein (GFP). Once ... Hastings, J.W.; Mitchell, G.W.; Mattingly, P.H.; Blinks, J.R.; Van Leeuwen, M. (1969). "Response of aequorin bioluminescence to ... and fishes with bacterial light organs. His laboratory produced the first evidence for quorum sensing in bacteria, early ... and the actual sub-cellular identity and location of the light emitting elements, which they termed scintillons. They ...
  https://en.wikipedia.org/wiki/John_Woodland_Hastings
*  GFP Introduction
... which is the blue light generated by purified aequorin. In vivo an energy transfer occurs from the excited state of the ... Upon mechanical stimulation A. victoria emits a green light. However, when the calcium-triggered photoprotein aequorin was ... rather than green light. GFP acts as a secondary fluorescent protein, receiving energy from activated aequorin. It has been ... Aequorin (shown on the very left) is a complex of the 21.4 kDa apoprotein, molecular oxygen and coelenterazine (an imidazole ...
  http://www.cryst.bbk.ac.uk/PPS2/projects/jonda/intro.htm
*  just another chemistry weblog | Category Archive | research
Eventually he found GFP, which fluoresces green when it absorbs the blue light emitted by aequorin. Chalfie genetically ... Shimomura originally discovered the GFP in a jellyfish, Aquoria victoria, and found that aequorin (the protein) emitted a blue ... Linked and knotted beams of light. third, we show that approximate knots of light may be generated using tightly focused ... The most popular trend in "new light bulbs" are compact fluorescent lights (CFLs), which only require about 20 to 25% of the ...
  http://www.justachemblog.net/category/research/page/2/
*  Aequorea victoria - Wikipedia
The blue light produced is in turn transduced to green by the now famous green fluorescent protein (GFP). Both aequorin and GFP ... This jellyfish is capable of producing flashes of blue light by a quick release of calcium (Ca2+) which interacts with the ... The species is best known as the source of two proteins involved in bioluminescence, aequorin, a photoprotein, and green ... PDB: 1EMA​ Shimomura O (August 1995). "A short story of aequorin". The Biological Bulletin. 189 (1): 1-5. doi:10.2307/1542194. ...
  https://en.wikipedia.org/wiki/Aequorea_victoria
*  Medjugorje is decadent and depraved: September 2013
... this Aequorin emits blue light and the GFP converts the blue light to green light which is what the eye sees... the resulting ... It is regulated in every emergency, not by fatuous, glimmering light of its own, or by worldly judgment, but by the maxims of ... in a sense Holy Prudence is a light to the reason..and as Jesus was described by John the evangelist as a light of which the ... It lights the way and measures the arena for their exercise. The insight it confers makes one distinguish successfully between ...
  http://medjugorje1.blogspot.com/2013/09/
*  Whitehead Institute - News - 2004 - Emerald City: How a jellyfish helped advance science
In a jellyfish, that source is the aequorin protein, which transfers its blue light to GFP by a quantum process that some ... whose cellular makeup includes a bioluminescent protein called aequorin that a emits a deep blue light. Another protein called ... In the laboratory, artificial light substitutes for aequorin's natural role.. Today, scientists use GFP to determine where ... causing those proteins to glow when illuminated with blue light. Under a microscope, these GFP-labeled proteins shine in a ...
  http://wi.mit.edu/news/archive/2004/emerald-city-how-jellyfish-helped-advance-science
*  GFP in Nature
... a blue light is produced. GFP absorbs the blue light completely and turns it into green light. The presence of 238 amino acids ... In Aequorea victoria jellyfish, the protein aequorin is present in the photo organs. When the protein binds with calcium, ... A similar light emitting method is seen in deep sea fish to attract and consume the prey. About 90% of deep ocean animals are ... In this rare property, upon exposing to light, the fluorescent emission will become bright and gradually reduced to a non- ...
  https://www.news-medical.net/life-sciences/GFP-in-nature.aspx
*  Three-Minute Anthrax Sensor
If the target agents are present, the cells emit blue light. The light in this image is a simulation; light emitted by cells in ... When activated by calcium in the cell, this enzyme, called aequorin, reacts with another jellyfish compound called ... If the agent the cells are designed to detect is present, they emit blue light. The detector uses software to analyze light ... One of the products of this reaction is blue light. (The Lincoln Lab researchers have yet to create B cells that can make their ...
  https://www.technologyreview.com/s/409759/three-minute-anthrax-sensor/amp/
*  Why study bioluminescence? | Marek Lab
... found that the jellyfish's bioluminescence is generated by Förster-type resonant energy transfer of aequorin's blue light to ... they observed blue light. In contrast, A. victoria glows green in nature. So how does the light change from blue to green? ... Aequorin is highly sensitive and specific to calcium and-most importantly-emits light when it reacts and thereby signals ... Turn off your flashlight and allow your eyes become adjusted to the light. Blue ghosts, railroad-worms, luminous millipedes, ...
  http://jointedlegs.org/?p=423
*  Blue Light Receptors and Signal Transduction | Plant Cell
... of cytosolic calcium was shown in transgenic plants expressing the recombinant calcium-binding fluorescent protein aequorin ( ... to mediate blue light induction of side branching of protonema, blue light stimulation of gametophore emergence, and blue light ... A plant blue light response was documented as early as 1881 by Darwin when he discovered what is now known as the blue light- ... Blue light-induced rapid plasma membrane depolarization is one of the early cellular blue light responses discovered (Spalding ...
  http://www.plantcell.org/content/14/suppl_1/S207.full?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=lin&andorexacttitle=and&andorexacttitleabs=and&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT
*  XpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™ from CHEMICON
... a blue light is emitted. This light is quantified using a microplate luminometer; the intensity of the light is directly ... a recombinant form of the photoprotein aequorin. Unbound conjugate is removed by washing. When calcium cations are added, ...
  http://www.bio-medicine.org/biology-products/XpressPack-26trade-3B-Luminescent-Amplification-Detection-System--required-for-luminescent-XpressPack-26trade-3B-from-CHEMICON-2520-1/
*  Bioreporter - Wikipedia
... a reaction occurs whose end result is the generation of blue light in the 460 - 470 nm range. Aequorin has been incorporated ... GFP, like aequorin, produces a blue fluorescent signal, but without the required addition of an exogenous substrate. All that ... Additionally, the ability to alter GFP to produce light emissions besides blue (i.e., cyan, red, and yellow) allows it to be ... have been isolated and used extensively in the construction of bioreporters that emit a blue-green light with a maximum ...
  https://en.wikipedia.org/wiki/Bioreporter
*  Bioluminescent mystery…….. | justanotherbiochemian
In the aequorin protein it causes the jellyfish to release flashes of bright blue light by releasing calcium, this is able to ... After this blue light is emitted it is transduced into green light by the green florescent protein (GFP). GFP is made up of 238 ... are very important as they absorb the blue light from the previously discussed protein aequorin and realse visible green light. ... This is now in its excited state and when it relaxes to the ground state, blue light is emitted. ...
  https://chelseaharripersad.wordpress.com/2013/03/24/bioluminescent-mystery/
*  luciferase (thing) by The Alchemist - Everything2.com
... and luciferases are a loose family of enzymes that are used by many organisms to produce light - a phenomenon ... ... This happens in some deep sea fish that need red light, but have blue-green light producing enzymes. ... An example of kind of light "transformation" is the Green Fluorescent Protein : aequorin pairing that is used in jellyfish. ... Lucifer means "light-bringer", and luciferases are a loose family of enzymes that are used by many organisms to produce light ...
  https://everything2.com/user/The+Alchemist/writeups/luciferase
*  CIENCIA: CHEMISTRY PRIZE NOBEL, 2008
... which after uniting with the protein aequorin, emitted blue light that turned green when being absorbed by the fluorescent ... As Tsien needed 2 colors of fluorescent proteins, he produce a mutation in the gene of GFP, obtaining brilliant blue light ... yellow in front of a bulb light and green fluorescent in front of UV light. Shimonura discovered that in order to produce ... Shimomura and Frank Johnson (Princeton), noticed that the protein acquired a green color in front of sun light, ...
  http://vmechanm.blogspot.com/2008/10/chemistry-prize-nobel-2008.html
*  Anti-GFP antibody (Biotin) [LGB-1] | Abcam
Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. ... Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.. Subunit structure: Monomer.. Tissue ... Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making ...
  http://www.abcam.com/gfp-antibody-lgb-1-biotin-ab42562.html
*  Aequorin-Containing Compositions and Methods of Using Same - Patent application
... dioxide and blue light. Aequorin is not exported or secreted by cells, nor is it compartmentalized or sequestered within cells ... The trypan blue exclusion method (healthy cells exclude trypan blue whereas dead or dying cells will take up the trypan blue ... 2, it is apparent that there are more trypan blue stained (dead) neurons in the control (upper panel) relative to the aequorin ... 0050]Aequorin is taken up by hippocampal neurons. In a set of preliminary studies, aequorin was bilaterally injected directly ...
  http://www.patentsencyclopedia.com/app/20100152117
*  Compositions, kits and methods for treatment of complement-related disorders - Patent application
... which emits blue light. Synthetic aequorin is commercially available from Sealite, Sciences (Bogart, Ga.) as AQUALITE®. GFP ... stained with trypan blue, and the right photomicrograph shows cells contacted with HI-NHS and then stained with trypan blue. ... Trypan Blue Exclusion Assay [0300] Cells were treated as for the MAC deposition assay in cell culture as described in Examples ... 3 panel C). No lysis was observed on HI-NHS exposed cells as indicated by the absence of trypan blue uptake by these cells (FIG ...
  http://www.patentsencyclopedia.com/app/20130143953