*  Structural Biochemistry/Proteins/Purification/SDS-Polyacrylamide Gel Electrophoresis - Wikibooks, open books for an open world
BN-Polyacrylamide Gel Electrophoresis[edit]. Similar to SDS-polyacrylamide gel electrophoresis, blue native-polyacrylamide gel ... Structural Biochemistry/Proteins/Purification/SDS-Polyacrylamide Gel Electrophoresis. From Wikibooks, open books for an open ... Biochemistry/Proteins/Purification/SDS-Polyacrylamide_Gel_Electrophoresis&oldid=2203427" ... SDS-Polyacrylamide Gel Electrophoresis is a technique to separate proteins according to elctrophoretic mobility - a function of ...
  https://en.wikibooks.org/wiki/Structural_Biochemistry/Proteins/Purification/SDS-Polyacrylamide_Gel_Electrophoresis
*  QPNC-PAGE - Wikipedia
McLellan T (1982). "Electrophoresis buffers for polyacrylamide gels at various pH". Analytical Biochemistry. 126 (1): 94-9. doi ... QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution and a highly ... Suck R, Petersen A, Weber B, Fiebig H, Cromwell O (2004). "Analytical and preparative native polyacrylamide gel electrophoresis ... "Gel composition in gels for submurged gel electrophoresis". Patent US5458760 (A). Westermeier R (2016). Electrophoresis in ...
  https://en.wikipedia.org/wiki/QPNC-PAGE
*  Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli | BMC Biochemistry...
For this purpose, we performed native and denaturing polyacrylamide gel electrophoresis of crude extracts and blotted them. ... Native gels (5% polyacrylamide, 37.5:1 acrylamide:bis in 50 mM MOPS-KOH, pH 7.0) and SDS gels (3-8% of polyacrylamide in 50 mM ... and RecC polypeptide was detected when the gels were probed with anti-RecC (Figures 2c, d). In the native gel, less ... were poured in gel cassettes [20]. The gels were prerun for 1 h, and samples were mixed with loading solution and run at 100 V ...
  https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-9-27
*  Characterization of auxiliary membrane proteins in the chloroplast of Arabidopsis thaliana
... fluorescence and the composition of their photosynthetic complexes was analysed by native polyacrylamide gel electrophoresis. ... Biochemistry and Molecular Biology Plant Biotechnology Research subject. Biochemistry Identifiers. URN: urn:nbn:se:umu:diva- ... Biochemistry and Molecular Biology Plant Biotechnology Research subject Biochemistry Identifiers. urn:nbn:se:umu:diva-125815 ( ... Biochemistry and Molecular Biology Identifiers. urn:nbn:se:umu:diva-124444 (URN)10.1016/j.bbabio.2016.05.004 (DOI) ...
  http://umu.diva-portal.org/smash/record.jsf?pid=diva2:972686
*  Polyacrylamide gel electrophoresis - Wikipedia
... quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE) is a method for separating native ... Polyacrylamide gel electrophoresis (PAGE), describes a technique widely used in biochemistry, forensics, genetics, molecular ... Polyacrylamide gel electrophoresis is a powerful tool used to analyze RNA samples. When polyacrylamide gel electrophoresis is ... Polyacrylamide gels are composed of a stacking gel and separating gel. Stacking gels have a higher porosity relative to the ...
  https://en.wikipedia.org/wiki/Polyacrylamide_gel_electrophoresis
*  B14. Advances in plant biochemistry. Lectures L27.1 L27.2. Mechanisms of chlorophyllase regulation - PDF
Advances in plant biochemistry Lectures L27.1 Polyisoprenoids secondary metabolites or physiologically important superlipids? ... Liliana Surmacz, Ewa Swiezewska Institute of Biochemistry and Biophysics, ... Sephacel anion-exchange chromatography and preparative polyacrylamide gel electrophoresis in non-denaturing conditions. 1-O-IA- ... We used native or denatured cytochrom b 6 : isolated from Synechocistis sp. PCC 6803 or overexpressed in E. coli and synthetic ...
  http://docplayer.net/739799-B14-advances-in-plant-biochemistry-lectures-l27-1-l27-2-mechanisms-of-chlorophyllase-regulation.html
*  Anti-p80 Ku Antibody, clone GE2.9.5 | 05-393
The purified enzyme showed equimolar amounts of 83- and 68-kDa polypeptides following polyacrylamide gel electrophoresis under ... Biochemistry, 33: 8548-57 (1994) 1994. Show Abstract A 150-kDa DNA-dependent ATPase composed of 83/68-kDa subunits was ... corresponding to a native molecular weight of 158 kDa. The antibodies raised in rabbit against either polypeptide can remove ... All these activities comigrate in gel filtration and glycerol gradients, giving a sedimentation coefficient of 7.4S and a ...
  http://www.merckmillipore.com/INTL/en/product/Anti-p80-Ku-Antibody-clone-GE2.9.5,MM_NF-05-393
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
In this video, we describe the analysis of MPCs by blue native polyacrylamide gel electrophoresis (BN-PAGE). BN-PAGE is a ... Biochemistry, Issue 48, BN-PAGE, 2D gel electrophoresis, cellular lysate, dialysis, protein complex, multiprotein complex, ... Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates ... A specialized machinery of molecular chaperones can bind non-native polypeptides and promote their folding into the native ...
  https://www.jove.com/visualize/abstract/26186340/proteasome-activity-is-affected-fluctuations-insulin-degrading-enzyme
*  Ionic Mobility and Electrophoresis - Chemistry LibreTexts
The most common mediums are agar and polyacrylamide gels.. Of key importance is the buffer used. Different compounds have ... Modern Experimental Biochemistry (Second Edition). Redwood City: The Benjamin/Cummings Publishing Company, Inc., 1993. ... different stability conditions, and the buffer must be chosen carefully so as not to "harm" the molecules' native states. The ... Concentration in terms of electrophoresis buffer refers to the ionic strength of the buffer; a buffer with a higher ionic ...
  https://chem.libretexts.org/Core/Analytical_Chemistry/Instrumental_Analysis/Capillary_Electrophoresis/Ionic_Mobility_and_Electrophoresis
*  Gel electrophoresis - Wikipedia
Proteins are usually analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), by native gel ... Gel electrophoresis is used in forensics, molecular biology, genetics, microbiology and biochemistry. The results can be ... Gel electrophoresis can also be used for separation of nanoparticles. Gel electrophoresis uses a gel as an anticonvective ... Discontinuous native protein gel electrophoresis Drinking straw electrophoresis How to run a DNA or RNA gel Animation of gel ...
  https://en.wikipedia.org/wiki/Gel_electrophoresis
*  Stability of the glycerol facilitator in detergent solutions. | Sigma-Aldrich
... polyacrylamide gel electrophoresis (PAGE), circular dichroism (CD), and fluorescence. Protein tetramers were prepared in ... Biochemistry 2008-2-21 Stability of the glycerol facilitator in detergent solutions.. [Jamie J Galka, Simon J Baturin, Darren M ... CD suggests that it forms a condensed structure with non-native tertiary contacts highly similar to the state observed in LMPC ... At pH 3 in DDM, the tetramer unfolds, losing its tertiary structure but retaining native helical structure which melts at ...
  https://www.sigmaaldrich.com/catalog/papers/18284214
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... of proteins and RNA molecules with rigorous purification schemes including denaturing polyacrylamide gel electrophoresis. In ... Genetics, Issue 73, Biochemistry, Molecular Biology, Neurobiology, Neuroscience, Bioengineering, Cellular Biology, Anatomy, ... but these approaches do not address RNA binding in its native cellular context. Initial attempts to study protein-RNA complexes ... Molecular Biology, Issue 78, Genetics, Biomedical Engineering, Medicine, Developmental Biology, Biochemistry, Anatomy, ...
  https://www.jove.com/visualize/abstract/22427994/muscleblind-like-1-knockout-mice-reveal-novel-splicing-defects
*  Isolation and Properties of Cytoplasmic α-Glycerol 3-Phosphate Dehydrogenase from the Pectoral Muscle of the Fruit Bat, Eidolon...
140 by SDS-polyacrylamide gel electrophoresis. The true Michaelis-Menten constants for all substrates at pH 7.5 were 3.9 ± 0.7 ... The apparent molecular weight of the native enzyme, as determined by gel filtration on Sephadex G-100 was 59,500 ± 650 daltons ... Journal of Biochemistry and Molecular Biology. Uncontrolled Keywords:. Animal/bat flight ; Fruit bat ; Glycerol-3-phosphate ... while only two of these residues were reactive towards DTNB in the native enzyme. The overall results of the in vitro ...
  http://eprints.lancs.ac.uk/9425/
*  Two-dimensional gel electrophoresis - Wikipedia
In native polyacrylamide gel electrophoresis (native PAGE), proteins remain in their native state and are separated in the ... "A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels". Analytical Biochemistry. 98 (1): ... Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used ... Gel IQ A freely downloadable software tool for assessing the quality of 2D gel image analysis data. 2-D Electrophoresis ...
  https://en.wikipedia.org/wiki/Two-dimensional_gel_electrophoresis
*  Protocols and Video Articles Authored by Peter-Leon Hagedoorn
... using native isoelectric focusing combined with blue native polyacrylamide gel electrophoresis into native-native 2D gel ... Function of MoaB Proteins in the Biosynthesis of the Molybdenum and Tungsten Cofactors Biochemistry. Jan, 2008 , Pubmed ID: ... From gel filtration and polyacrylamide gel electrophoresis it was concluded that FDH-1 is composed of three subunits (89 +/- 3 ... Application of the radioisotope (99)Mo in metal isotope native radioautography in gel electrophoresis (MIRAGE) technology to P ...
  https://www.jove.com/author/Peter-Leon_Hagedoorn
*  Coomassie Brilliant Blue - Wikipedia
... or protein complexes using polyacrylamide gel electrophoresis under non-denaturing conditions in a technique called Blue Native ... "Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form". Analytical Biochemistry ... They soaked the gel in a dye solution containing methanol, acetic acid and water. As the dye stained the polyacrylamide gel as ... Altschul, A. M.; Evans, W. J. (1967). "Zone electrophoresis with polyacrylamide gel". Methods in Enzymology. Methods in ...
  https://en.wikipedia.org/wiki/Coomassie_Brilliant_Blue
*  Apparent isocitrate lyase activity in Leishmania amazonensis : Acta Parasitologica
Activity staining of isocitrate lyase after electrophoresis on either native or sodium dodecyl sulfate polyacrylamide gels. ... Comparative biochemistry of the glyoxylate cycle. Comparative Biochemistry and Physiology, 70B, 1-26. CrossrefGoogle Scholar ... Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. ... Archives of Biochemistry and Biophysics, 399, 225-231. CrossrefPubMedGoogle Scholar ...
  https://www.degruyter.com/view/j/ap.2017.62.issue-4/ap-2017-0084/ap-2017-0084.xml
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The formation of RIG-I and PKR oligomers was validated by native polyacrylamide gel electrophoresis (PAGE). Upon infection, ... Biochemistry, Issue 55, glucocorticoid receptor, hsp90, molecular chaperone protein, in vitro reconstitution, steroid binding, ... Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells. Authors: M. Dean Chamberlain, Mark J. Butler, Ema ... IP-FCM is extremely sensitive, allows analysis of proteins in their native (non-denatured) state, and is amenable to either ...
  https://www.jove.com/visualize/abstract/21779388/a-conformation-sensitive-monoclonal-antibody-against-a2-domain-von
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The formation of RIG-I and PKR oligomers was validated by native polyacrylamide gel electrophoresis (PAGE). Upon infection, ... Biochemistry, Issue 82, amyloid, soluble protein oligomer, amyloid precursor, protein misfolding, amyloid fibril, protein ... Although the native structures of polypeptides fulfill many purposes, it is now recognized that most proteins can adopt an ... Limited protease digestion and native PAGE, both coupled to western blot analysis, allow a sensitive and direct measurement of ...
  https://www.jove.com/visualize/abstract/25062013/granulocyte-colony-stimulating-factor-attenuates-oligomeric-amyloid
*  Alfred M. Spormann | Stanford Medicine Profiles
Native polyacrylamide gel electrophoresis and mass spectrometry revealed that a trichloroethene reductive dehalogenase (TceA) ... Biochemistry of Catabolic Reductive Dehalogenation. Annual review of biochemistry Fincker, M., Spormann, A. M. 2017; 86: 357-86 ... The biochemistry of anaerobic toluene degradation has been studied in detail. Toluene is activated by addition to fumarate to ... Visiting Professor, Department of Biochemistry and Biological Process Institute, University of Minnesota (1997) ...
  https://med.stanford.edu/profiles/alfred-spormann
*  Gene Report for G00001754 - Genes2Cognition Neuroscience Research Programme
Using two-dimensional Blue Native polyacrylamide gel electrophoresis/sodium dodecyl sulfate-polyacrylamide gel electrophoresis ... Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and ... as determined by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Co-purification of these components ... Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry.. Ahmed M, Forsberg J ...
  http://www.genes2cognition.org/db/Gene/G00001754
*  Vesicular Monoamine Transporters | Application of Computational Methods for the Design of BACE-1 Inhibitors
Immunoblot evaluation of one-dimensional Blue-Native polyacrylamide gel electrophoresis (BN-PAGE) gels demonstrated marked ... Outcomes Biochemistry Spectrophotometric evaluation of respiratory string enzyme activities uncovered severe isolated scarcity ... Rings were excised through the gel and sequenced in that case. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and ... Protein had been then loaded inside a 12% NuPAGE Novex gel (Invitrogen) and work for 1 h at 200V. For Traditional western ...
  http://world-of-links.com/category/vesicular-monoamine-transporters/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Naturally occurring Aβ multimers can be detected by SDS-polyacrylamide gel electrophoresis followed by immunoblotting with Aβ- ... Although the native structures of polypeptides fulfill many purposes, it is now recognized that most proteins can adopt an ... Biochemistry, Issue 82, amyloid, soluble protein oligomer, amyloid precursor, protein misfolding, amyloid fibril, protein ... Abbreviations: 2DE (two-dimensional gel electrophoresis), 2D-DIGE (two-dimensional fluorescence difference gel electrophoresis ...
  https://www.jove.com/visualize/abstract/23028511/plasma-based-markers-of-11c-pib-pet-brain-amyloid-burden
*  Patent US5643722 - Methods for the detection and isolation of proteins - Google Patents
tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or ... Proteins in the cell-free extract are separated by polyacrylamide gel electrophoresis (PAGE). The resulting gel contains bands ... Biochemistry 30:5411-21, 1991). Furthermore, site-specific incorporation of non-native amino acids is not suitable in general ... polyacrylamide gel with a 3% stacker. The water cooled gel was electrophoresed for 4 hours at 50 volts. After electrophoresis, ...
  http://www.google.ca/patents/US5643722
*  SDS-PAGE - Wikipedia
... is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged ... Two-dimensional gel electrophoresis sequentially combines isoelectric focusing or BAC-PAGE with a SDS-PAGE. Native PAGE is used ... When using different buffers in the gel (discontinuous gel electrophoresis), the gels are made up to one day prior to ... For the gel solution, acrylamide is mixed as gel-former (usually 4% V/V in the stacking gel and 10-12 % in the separating gel ...
  https://en.wikipedia.org/wiki/SDS-PAGE