*  Discovery Biology News
... or as a new bi-specific antibody therapeutic.. Camelid single domain antibodies are a novel bi-specific antibody platform that ... With our one-stop services, we are determined to be your best partner in antibody drug discovery from gene synthesis to in-vivo ... Epitope specificity plays an important role in regulating antibody-dependent cell-mediated cytotoxicity. In addition, it is ... Thus, combination therapy can be designed by targeting different binding sites. Furthermore, the antibody is capable of getting ...
  https://www.genscript.com/DiscoveryBiology_news.html
*  IJMS | Free Full-Text | Antibody Phage Display Libraries: Contributions to Oncology | HTML
Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the ... Examples of such antibodies will be discussed here with emphasis on those used as probes for molecular imaging and other ... Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any ... antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different ...
  http://www.mdpi.com/1422-0067/13/5/5420/htm
*  Single-domain antibody - Wikipedia
... it is able to bind selectively to a specific antigen. With a molecular weight of only 12-15 kDa, single-domain antibodies are ... Single-domain antibodies allow a broad range of applications in biotechnical as well as therapeutic use due to their small size ... By reverse transcription and polymerase chain reaction, a gene library of single-domain antibodies containing several million ... "A human single-domain antibody elicits potent antitumor activity by targeting an epitope in mesothelin close to the cancer cell ...
  https://en.wikipedia.org/wiki/Single-domain_antibody
*  Protocols and Video Articles Authored by Kenny Roose
We conclude that single-domain antibodies directed against influenza virus NA protect against H5N1 virus infection, and when ... We show here that two M2e-specific monoclonal antibodies with close to identical antigen-binding specificities and affinities ... which recognize a similar epitope in M2e with similar affinities. The binding of MAb 65 to influenza A virus-infected cells ... However, reporter gene-expressing influenza viruses are often attenuated in vivo and may be genetically unstable. Here, we ...
  https://www.jove.com/author/Kenny_Roose
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The antibody against the Uni-1 epitope of HA was able to bind to 13 subtypes of influenza A HA (H1-H13) while the antibodies ... However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... transient protein production in plants is a promising approach to produce vaccine antigens and therapeutic proteins within a ... Single cell codetection of a gene, its RNA product and cellular regulatory proteins is critical to study gene expression ...
  https://www.jove.com/visualize/abstract/24751715/development-vhh-antibodies-against-dengue-virus-type-2-ns1-comparison
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The antibody against the Uni-1 epitope of HA was able to bind to 13 subtypes of influenza A HA (H1-H13) while the antibodies ... However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation ... Single cell codetection of a gene, its RNA product and cellular regulatory proteins is critical to study gene expression ...
  https://www.jove.com/visualize/abstract/24069315/anti-trypanosoma-cruzi-cross-reactive-antibodies-detected-at-high
*  Methods for detecting and treating cancer - The General Hospital Corporation
8(10): 1057-1062, 1995), single domain antibodies, single chain antibodies, and antibodies having multiple valencies (e.g., ... retains one of the antigen binding sites of the intact antibody. Fab′ fragments consist of a covalently bound antibody light ... is that nanobodies can bind therapeutic targets not accessible to conventional antibodies. Recombinant antibody fragments with ... Genes from the human antibody gene libraries are inserted into a population of phage. Each phage carries the genes for a ...
  http://www.freepatentsonline.com/9885720.html
*  COMPOSITIONS AND METHODS OF USE FOR THERAPEUTIC ANTIBODIES - Patent application
... "antigen-binding portion") or single chains thereof. A binding protein comprising the antigen-binding portion of an antibody is ... CH1 or CL region to contain a salvage receptor binding epitope taken from two loops of a CH2 domain of an Fc region of an IgG, ... The antibody light chain gene and the antibody heavy chain gene can be inserted into separate vector or, more typically, both ... They are derived from lipocalins, a widespread group of small and robust proteins that are usually involved in the ...
  http://www.patentsencyclopedia.com/app/20120034231
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes ... Thus, this technique can be established for medium to high throughput screening of antigens and antibodies, such as newly- ... Here we present a novel method for assessing the in vitro replication of HIV-1 as influenced by the gag gene isolated from ...
  https://www.jove.com/visualize/abstract/21297864/heterologous-epitope-scaffold-primeboosting-immuno-focuses-b-cell
*  DiVA - Search result
Tumor cell targeted gene delivery by adenovirus 5 vectors carrying knobless fibers with antibody-binding domains2005In: Gene ... epitope recognized by the monoclonal antibody C242) antigen on HT29 and SW1116, and PSMA ( prostate-specific membrane antigen) ... Due to their small size in comparison to immunoglobulins, such single domain binding proteins may be suitable for high ... The use of this type of small, robust, and easily expressed anti-idiotypic affibody pair for affinity technology applications, ...
  http://kth.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22personId%22%3A%22authority-person%3A31099+OR+0000-0003-4214-6991%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
*  Crystal Structure of the Amyloid-β p3 Fragment Provides a Model for Oligomer Formation in Alzheimer's Disease | Journal of...
... observed that the 12Y-2 IgNAR single domain antibody crystal structures comfortably accommodated the extended antigen-binding β ... the immunodominant B-cell epitope of the Aβ1-8 fragment in complex with murine antibodies, which revealed extended coil-like ... Aβ-IgNAR gene constructs were produced by splice-overlap PCR as previously described (Henderson et al., 2007) using IgNAR 12Y-2 ... 1C). The interchain association for each dimer (A-C and B-D) is extremely robust, as indicated by an average surface of ...
  http://www.jneurosci.org/content/31/4/1419.long
*  Plus it
... observed that the 12Y-2 IgNAR single domain antibody crystal structures comfortably accommodated the extended antigen-binding β ... the immunodominant B-cell epitope of the Aβ1-8 fragment in complex with murine antibodies, which revealed extended coil-like ... Aβ-IgNAR gene constructs were produced by splice-overlap PCR as previously described (Henderson et al., 2007) using IgNAR 12Y-2 ... 1C). The interchain association for each dimer (A-C and B-D) is extremely robust, as indicated by an average surface of ...
  http://www.jneurosci.org/content/31/4/1419
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... a single user can generate hundreds of antigens, select antibodies to them in parallel and validate antibody binding within 6-8 ... antibodies specific for OmpL5410 are detetcted aftero binding to native, surface exposed epitopes. Comparison of antibody ... However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... An amber mutation is first introduced at a specific codon of the gene encoding the protein of interest. The amber mutant is ...
  https://www.jove.com/visualize/abstract/25811612/inhibition-of-type-vi-secretion-by-an-anti-tssm-llama-nanobody
*  B cell repertoires in HLA-sensitized kidney transplant candidates undergoing desensitization therapy | Journal of Translational...
Decanniere K, Desmyter A, Lauwereys M, Ghahroudi MA, Muyldermans S, Wyns L. A single-domain antibody fragment in complex with ... Antigen-specific B cells recognize, bind, internalize and process the antigens through the B cell receptor (BCR) [6]. When a co ... HLA epitopes as viewed by antibodies: what is it all about? Am J Transplant. 2015;15:1148-54.View ArticlePubMedGoogle Scholar. ... Weigert M, Perry R, Kelley D, Hunkapiller T, Schilling J, Hood L. The joining of V and J gene segments creates antibody ...
  https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1118-7
*  Microbial Cell Factories - ISSN: 1475-2859
Biotechnological applications of recombinant single-domain antibody fragments Author(s): de Marco Ario. Volume: 10. Issue: 1. ... binding and increases heparin binding capacities Author(s): Ueda Eric , Soares Carlos , Walker Ameae , Bartolini Paolo. Volume ... Impact of apoptosis gene targeting on recombinant protein glycosylation Author(s): Wong Danny , Wong Niki , Goh John , Yap ... Pseudomonas fluorescens - a robust expression platform for pharmaceutical protein production Author(s): Retallack Diane , ...
  http://journaldatabase.info/journal/issn1475-2859
*  Neurodegenerative Diseases | Leaders in Pharmaceutical Business Intelligence (LPBI) Group
a single antibody.. In the present study, the ASD1 peptoid binds significantly lower levels of IgG1 in ASD males vs. TD males. ... Small Molecules in Development of Therapeutic Drugs, Electrophysiology, Gene Regulation, Genetics & Pharmaceutical, Genome ... Choosing the right antibody platform can speed the discovery of a new therapeutics against these new targets. Single domain ... do not develop NFTs or robust neurodegeneration as observed in human patients, despite robust Aβ deposition 13,22,23. Double ...
  https://pharmaceuticalintelligence.com/category/neurodegenerative-diseases/
*  MULTIPLE REACTION MONITORING LC-MS/MS METHOD TO DETECT THERAPEUTIC ANTIBODIES IN ANIMAL SAMPLES USING FRAMEWORK SIGNATURE...
A single VH or VL domain may be sufficient to confer antigen-binding specificity. Furthermore, antibodies that bind a ... known antibody for binding to antigen. In certain embodiments, such a competing antibody binds to the same epitope (e.g., a ... The robust format allows ready lab-to-lab transfer, a significant advantage for approved antibody therapies. A general ... 0144] (32) CD72 (B-cell differentiation antigen CD72, Lyb-2); 359 aa, pI: 8.66, MW: 40225, TM: 1 [P] Gene Chromosome: 9p13.3, ...
  http://www.patentsencyclopedia.com/app/20120315645
*  SYNERGISTIC TREATMENT OF CELLS THAT EXPRESS EPHA2 AND ERBB2 - Patent application
... the desired binding specificities (antibody-antigen combining sites) are fused to immunoglobulin constant domain sequences. The ... predetermined mixture of antibodies against one or more epitopes are generated in a single cell. Oligoclonal antibodies may ... 0119]Techniques for conjugating therapeutic moieties to antibodies are well known. Moieties can be conjugated to antibodies by ... 1997) Gene Therapy 4:1004-1012; Douglas et al. (1996) Nature Biotechnology 14: 1574-1578). For example, an antibody or an ...
  http://www.patentsencyclopedia.com/app/20100254996
*  Kim, MA - Patent applications
COMPOSITIONS AND METHODS FOR ANTIBODIES TARGETING FACTOR P - The present invention relates to antibodies or antigen binding ... An OFDM communication system is described that allows different values of D in a single domain where nodes are operating in ... Molecules and Methods for Modulating Complement Component - Compositions that bind to C3b epitopes and methods of using the ... ROBUST PREAMBLE FOR COMMUNICATION OVER NOISY MEDIA - Described herein are techniques related to data communications using a ...
  http://www.patentsencyclopedia.com/inventor/kim-ma-3/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
In tissues that express the Cre recombinase it binds to target sequences and excises the floxed gene. Controlled gene deletion ... The immunoblotting technique has been useful in identifying specific antigens recognized by polyclonal or monoclonal antibodies ... One such domain is the plant homeodomain (PHD), found in several chromatin-binding proteins. The epigenetic factor RBP2 has ... FIPA analysis also introduces the option to bind more than one differently tagged AFP on the same single ice crystal to help ...
  https://www.jove.com/visualize/abstract/24603559/specificity-non-isoforms-arabidopsis-14-3-3-proteins-towards-h-atpase
*  Recombinant Immunotoxin Therapy of Glioblastoma: Smart Design, Key Findings, and Specific Challenges
Single-chain antigen-binding proteins. Science. 1988;242(4877):423-426. doi: 10.1126/science.3140379. [PubMed] [Cross Ref] ... Identification of the epitope for the epidermal growth factor receptor-specific monoclonal antibody 806 reveals that it ... one is the receptor binding domain (R domain) that enables toxin to be absorbed on cell surface; one is the translocation ... A single intratumoral injection of such a therapeutic vector into intracranial human GBM xenografts and murine GL26 tumors in ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC5511670/
*  Otto Hannes Vogel | Stanford Medicine Profiles
... a bispecific antibody reduced tumorigenicity of implanted tumor cells better than any reagent directed against a single epitope ... We find that p53 transactivates FoxO3 in cells by binding to a site in the second intron of the FoxO3 gene, a genomic region ... This approach functionally identifies chromodomain helicase DNA binding domain 5 (Chd5) as a tumor suppressor that controls ... Here we show that TAp63 isoforms are robust mediators of senescence that inhibit tumorigenesis in vivo. Whereas gain of TAp63 ...
  https://med.stanford.edu/profiles/hannes-vogel
*  Early detection of diabetes - The Johns Hopkins University
As used herein, the term "epitope" means any antigenic determinant on an antigen to which the paratope of an antibody binds. ... 279, 20636-20642 (2004)). A single-nucleotide polymorphism commonly occurs in the MGEA5 gene (which encodes O-GlcNAcase) within ... The goal of this exercise it to validate that it is possible to use the antibodies to produce a robust, simple assay in which ... 4. The method of claim 1, wherein the second antibody is detectably labeled. 5. A method of monitoring a therapeutic regimen ...
  http://www.freepatentsonline.com/9250248.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
It relies on anti-GLUT4 antibodies directed to an external epitope of the transporter that bind to it as soon as it is exposed ... This antigen transcytosis is mediated by specialized epithelial M cells3, 4. M cells are atypical epithelial cells that ... The formation of LCVs represents a complex, robust and redundant process, which is difficult to grasp in a reductionist manner ... This technique highlights and relies on the contribution of host cell-dependent gene expression by mimicking the environment ...
  https://www.jove.com/visualize/abstract/24040297/cag-type-iv-secretion-system-cagi-independent-bacterial-surface
*  Patente US20100092475 - Treatment method using egfr antibodies and src inhibitors and related ... - Google Patentes
Methods and compositions for treatment of cancer with the antibody anti-EGFR mAb806 in combination or series with a src ... The invention further relates to the treatment of cancer with anti-EGFR antibodies and src inhibitors. ... an antibody may bind to a particular part of the antigen only, which part is termed an epitope. An antigen binding domain may ... A monoclonal antibody thus typically displays a single binding affinity for any antigen with which it immunoreacts. A ...
  http://www.google.es/patents/US20100092475