*  An antibody binding site on cytochrome c defined by hydrogen exchange and two-dimensional NMR | Science
An antibody binding site on cytochrome c defined by hydrogen exchange and two-dimensional NMR ... The hydrogen exchange labeling approach can be used to map binding sites on small proteins in antibody-antigen complexes and ... An antibody binding site on cytochrome c defined by hydrogen exchange and two-dimensional NMR ... An antibody binding site on cytochrome c defined by hydrogen exchange and two-dimensional NMR ...
  http://science.sciencemag.org/content/249/4970/755
*  Targeting Ligand-Induced Binding Sites on GPIIb/IIIa via Single-Chain Antibody Allows Effective Anticoagulation Without...
Targeting Ligand-Induced Binding Sites on GPIIb/IIIa via Single-Chain Antibody Allows Effective Anticoagulation Without ... Targeting Ligand-Induced Binding Sites on GPIIb/IIIa via Single-Chain Antibody Allows Effective Anticoagulation Without ... Targeting Ligand-Induced Binding Sites on GPIIb/IIIa via Single-Chain Antibody Allows Effective Anticoagulation Without ... Targeting Ligand-Induced Binding Sites on GPIIb/IIIa via Single-Chain Antibody Allows Effective Anticoagulation Without ...
  http://atvb.ahajournals.org/content/early/2007/02/22/ATVBAHA.106.138875
*  Targeted N-glycan deletion at the receptor-binding site retains HIV Env NFL trimer integrity and accelerates the elicited...
N-glycan deletion at the receptor-binding site retains HIV Env NFL trimer integrity and accelerates the elicited antibody ... We focused on the conserved CD4 binding site (CD4bs) since this is a known neutralizing determinant that is devoid of ... Use of this site constitutes acceptance of the PhysiciansWeekly.com Terms of Use and Privacy Policy. The material on this site ... The first was to delete 4 PNGS sites and then boost with fully glycosylated Env; the second was to delete 4 sites and gradually ...
  https://www.physiciansweekly.com/targeted-n-glycan-deletion-at-the-receptor-binding-site-retains-hiv-env-nfl-trimer-integrity-and-accelerates-the-elicited-antibody-response-2/
*  Antigen-binding sites dominate the surface properties of antibodies - Lund University
Antigen-binding sites dominate the surface properties of antibodies. Research output: Contribution to journal › Article ... Hence, the surface properties of the antigen binding sites dominate over all other surfaces of the free antibody molecule.. ... HomeResearch Outputs Antigen-binding sites dominate the surface properties of ant... ... LLPC may also be used to detect conformational changes occuring upon binding of antigen by antibody. Antigen-antibody complexes ...
  http://portal.research.lu.se/portal/en/publications/antigenbinding-sites-dominate-the-surface-properties-of-antibodies
*  IDEALS @ Illinois: Antibody active site contributions to fluorescein ligand binding
Antibody active site contributions to fluorescein ligand binding. Welcome to the IDEALS Repository. ... responsible for the observed Fl binding characteristics. To begin active site structure-function assessments, V$\sb{\rm H}$ and ... To verify this, Fl binding assays, following in vitro H and L chain reassociations, were performed. Results indicated: (1) L34 ... A panel of idiotypically cross-reactive murine monoclonal antibodies (Mabs) isolated in response to fluorescein (Fl) was ...
  https://www.ideals.illinois.edu/handle/2142/23366
*  Patent US6242570 - Production and use of recombinant protein multimers with increased ... - Google Patents
Biosynthetic antibody binding sites. US5114711. Jul 13, 1989. May 19, 1992. G. D. Searle & Co.. Covalently linked polypeptide ... such as necessary ribosome binding sites, a poly-adenylation site, splice donor and acceptor sites, and transcriptional ... Feldman, L., et al., "Four Unique Monoclonal Antibodies to the Putative Receptor Binding Domain of Erythropoietin Inhibit the ... Half a NaeI restriction site was designed into the 3′ end of EPO A DNA and half a ScaI restriction site into the 5′ end of EPO ...
  http://www.google.com/patents/US6242570
*  Patente US8093043 - β-TrCP1, β-TrCP2 and RSK1 or RSK2 inhibitors and methods for sensitizing ... - Google Patentes
Biosynthetic antibody binding sites. US5168062. 10 Sep 1990. 1 Dic 1992. University Of Iowa Research Foundation. Transfer ... antibody binding is detected by detecting a label on the primary antibody. In another embodiment, the primary antibody is ... Also, the primary antibody was added. The dilution of antibody used was antibody-dependent. The range of dilution was from 1:5 ... assays that measure selective binding of test compounds to BimEL, to the BimEL binding site on β-TrCP1/2, or RSK1/2 or another ...
  http://www.google.es/patents/US8093043?hl=es&dq=flatulence
*  Patent US5395754 - Membrane-based immunoassay method - Google Patents
... first binding pair being covalently cross-linked such that any remaining binding sites on said first immobilized antibody are ... labelled second antibody specifically bound in the test zone versus the amount of labelled second antibody specifically bound ... analyte in the test and calibrator zones with a labelled second antibody capable of binding to a second antigen site on the ... but at least some of said analyte is capable of specifically binding to a preselected amount of a labelled second antibody. The ...
  http://www.google.com.au/patents/US5395754
*  In Vivo Imaging Using Quantum Dot-Conjugated Probes - Current Protocols
... analysis of ligand binding constants and receptor density ... Cytometric Quantitation of the Number of Antibody Binding Sites ... Basic Protocol 3: Flow Cytometric Quantitation of the Number of Antibody Binding Sites. ... Basic Protocol 2: Flow Cytometric Determination of the Binding Constants of Ligands or Antibodies. ... Basic Protocol 2: Flow Cytometric Determination of the Binding Constants of Ligands or Antibodies ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-cb2501.html
*  Autoantibodies to the extracellular matrix microfibrillar protein, fibrillin 1, in patients with localized scleroderma.
Binding Sites, Antibody / immunology. Humans. Immunoglobulin G / metabolism. Immunoglobulin M / metabolism. Microfilament ... 0/Autoantibodies; 0/Binding Sites, Antibody; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Microfilament Proteins; 0/fibrillin ... No correlations between antifibrillin 1 antibodies and active skin disease or antinuclear antibody positivity were found. ... This study was undertaken to determine whether antifibrillin 1 antibodies could be detected in patients with localized forms of ...
  http://www.biomedsearch.com/nih/Autoantibodies-to-extracellular-matrix-microfibrillar/10616014.html
*  Autoantibodies bind solubilized calcium channel-omega-conotoxin complexes from small cell lung carcinoma: a diagnostic aid for...
... bound specifically to a soluble omega-conotoxin binding component of a voltage-gated Ca2+ channel (VGCC) complex extracted from ... Binding Sites, Antibody / immunology, physiology. Calcium Channels / metabolism*. Carcinoma, Small Cell / immunology*. ... bound specifically to a soluble omega-conotoxin binding component of a voltage-gated Ca2+ channel (VGCC) complex extracted from ... Autoantibodies bind solubilized calcium channel-omega-conotoxin complexes from small cell lung carcinoma: a diagnostic aid for ...
  http://www.biomedsearch.com/nih/Autoantibodies-bind-solubilized-calcium-channel/2557495.html
*  Patent US7078492 - Human antipneumococcal antibodies from non-human animals - Google Patents
The invention further provides methods for making the antibodies in a non-human animal and for expressing the antibodies in ... Kits and pharmaceutical compositions comprising the antibodies are also provided in addition to methods of treating, inhibitng ... The invention described herein provides human antibodies produced in non-human animals that specifically bind to Streptococcus ... Thus, an intact IgG antibody has two binding sites. Except in bifunctional or bispecific antibodies, the two binding sites are ...
  http://www.google.com/patents/US7078492?dq=5910382
*  Anti-PBR antibody [EPR5384] KO Tested Recombinant (ab109497) | Abcam
Rabbit recombinant monoclonal PBR antibody [EPR5384] validated for WB, IP, IHC, Flow Cyt, ICC/IF and tested in Human and Mouse ... Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding ... Primary antibodies. Secondary antibodies. ELISA, Matched Antibody Pairs and Multiplex Immunoassays. Cell and tissue imaging ... Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500 ...
  http://www.abcam.com/pbr-antibody-epr5384-ab109497.html?productwalltab=abreviews
*  Paper Chase | HMS
Natively glycosylated HIV-1 Env structure reveals new mode for antibody recognition of the CD4-binding site. Nat. Struct. Mol. ... N-linked glycosylation of SV2 is required for binding and uptake of botulinum neurotoxin A. Nat. Struct. Mol. Biol. ... p53 pulses lead to distinct patterns of gene expression albeit similar DNA-binding dynamics. Nat. Struct. Mol. Biol. ...
  https://hms.harvard.edu/research/paper-chase?pcs=3&pcq=Nature%20Structural%20and%20Molecular%20Biology
*  Previous Exam 4
An epitope is the specific binding site on an antibody.. Monoclonal antibodies will bind to only one epitope. ... or analysis of this antibody and the design of the assay for kangaroo meat in hamburger..(15 pts.) ...
  http://www.uky.edu/Ag/FoodScience/test4-535.html
*  Patent US20050032144 - Method for selective conjugation of analytes to enzymes without unwanted ... - Google Patents
Essentially, there is a "competition" for binding sites of the antibody. The amount of free cAMP in the sample is inversely ... binds to the bottom of the well in the microtiter plate, the primary antibody (rabbit-anti-cAMP) binds to the capture antibody ... The more free cAMP that is present the less cAMP conjugate that can bind to the antibody and the reverse is also true. ... Google Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send Feedback ...
  http://www.google.com.au/patents/US20050032144
*  Angiotensin II Stimulates Gene Expression of Cardiac Insulin-Like Growth Factor I and Its Receptor Through Effects on Blood...
The antibody binding sites were visualized with fast red (Vector Laboratories). The sections were counterstained with Harris' ... formaldehyde and labeled with a polyclonal antibody to IGF-I followed by a goat anti-rabbit biotin-labeled secondary antibody. ... IGF-I polyclonal antibody UB 286 (raised by Drs L.E. Underwood and J.J. van Wyk, University of North Carolina, Chapel Hill) was ... thought you would like to see the Hypertension web site. ... Insulin-like growth factors and their binding proteins: ...
  http://hyper.ahajournals.org/content/34/5/1053
*  Sandwich Assay for Tacrolimus Using 2 Antitacrolimus Antibodies | Clinical Chemistry
Overlaps between the 1E2 binding-aid sites and 14H04 binding sites (C15 and C31) and overlaps between 14H04 binding-aid sites ... Their cross-reactivity reflects analog-antibody binding relative to drug-antibody binding. The difference in antibody binding ... This is especially true if the deviation occurs at the antibody binding site. A binding site is indicated if a structural ... To deduce the binding sites for an antibody in the absence of the x-ray cocrystal structure of antibody-drug complexes, we ...
  http://clinchem.aaccjnls.org/content/60/4/621.full
*  RomesbergLab | Publications
Flexibility of an antibody binding site measured with photon echo spectroscopy. The reorganization energies of the chromophore− ... We use photon echo spectroscopy to measure the response of three antibody-binding sites to perturbation from electronic ... On the basis of isothermal titration calorimetry, we select six antibodies that bind fluorescein with diverse binding entropies ... A molecular description of flexibility in an antibody combining site. We show that 3PEPS spectroscopy in combination with ...
  http://www.scripps.edu/romesberg/publications.html
*  PDGF C Is A Selective α Platelet-Derived Growth Factor Receptor Agonist That Is Highly Expressed in Platelet α Granules and...
Identification of PDGF binding site and neutralizing monoclonal antibodies. J Biol Chem. 1997; 272: 33037-33044. ... Therefore, we used a 2-site enzyme-linked immunosorbent assay to measure α/β PDGFR heterodimerization using specific antibodies ... Antibody Production. Anti-PDGF C monoclonal antibodies were generated using standard techniques.12 BALB/c mice were immunized ... Your Name) thought you would like to see the Arteriosclerosis, Thrombosis, and Vascular Biology web site. ...
  http://atvb.ahajournals.org/content/24/4/787
*  Envelope glycoprotein GP120 - Wikipedia
Many neutralizing antibodies bind to sites located in variable regions of gp120, so mutations in these regions will be selected ... The presence of large carbohydrate chains extending from gp120 might obscure possible antibody binding sites. The boundaries of ... "Fine mapping of the interaction of neutralizing and nonneutralizing monoclonal antibodies with the CD4 binding site of human ... "Global Shape and Ligand Binding Efficiency of the HIV-1-neutralizing Antibodies Differ from Those of Antibodies That Cannot ...
  https://en.wikipedia.org/wiki/Envelope_glycoprotein_GP120
*  Search
The development of CD4 binding site antibodies during HIV-1 infection. Lynch, Rebecca M.; Tran, Lillian.; Louder, Mark K.; ... Broadly neutralizing antibodies to the CD4 binding site (CD4bs) of gp120 are generated by some HIV-1-infected individuals, but ... Identifying the targets of broadly neutralizing antibodies to HIV-1 and understanding how these antibodies develop remain ... and IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia. Tomaras, Georgia D.; ...
  http://researchspace.ukzn.ac.za/handle/10413/7813/discover?filtertype=subject&filter_relational_operator=equals&filter=HIV+infections--Immunology.
*  Use of colloidal coomassie stained to western blotting?? - SDS-PAGE and Western Blotting
if u just wanna identify ur protein of interest then u can go ahead.. hoping that the antibody binding site is secure!!! ... the coomassie will, at least partially, come off the protein when transferred but will bind to the membrane.. ... the coomassie will, at least partially, come off the protein when transferred but will bind to the membrane. ...
  http://www.protocol-online.org/biology-forums-2/posts/22090.html
*  Combinatorial depletion analysis to assemble the network architecture of the SAGA and ADA chromatin remodeling complexes |...
This website is best viewed using the latest versions of all modern web browsers. Older browsers may not display correctly. ... The next day, the beads were washed three times with 300 mM calmodulin‐binding buffer, then 2 × SDS sample was added and the ... we immunoprecipitated yeast containing a TAP tag on Ahc2 or Sgf29 and probed with an antibody to Ada3, a known component of the ... If SAGA is present, Gal4‐VP16 bound to the GAL1 promoter drives LacZ expression. ...
  http://msb.embopress.org/content/7/1/503