*  E2F4 Modulates Differentiation and Gene Expression in Hematopoietic Progenitor Cells during Commitment to the Lymphoid Lineage ...
This leads to the binding of E2F factors, including E2F4. The active E2F transcription factors E2F1, E2F2, and E2F3a are ... E2F4 and E2F5, have the ability to bind similar promoter regions to those bound by the activating E2Fs (11), but are ... Thus, we crossed E2F4 heterozygous mice to generate fetuses of all three genotypes: e2f4+/+, e2f4+/− and e2f4−/−. Fetuses were ... e2f4+/− (CD45.2+) cells mixed with e2f4+/+ (CD45.1+) cells, or e2f4−/− (CD45.2+) cells mixed with e2f4+/+ (CD45.1+) cells. ...
  http://www.jimmunol.org/content/180/6/3699.long
*  E2F - Wikipedia
... analysis has shown that the E2F family of transcription factors has a fold similar to the winged-helix DNA-binding motif. E2F ... to the promoter. E2F3b, E2F4, E2F5 are expressed in quiescent cells and can be found associated with E2F-binding elements on ... Binding with DP1,2 provides a second DNA binding site, increasing E2F binding stability. Most E2F have a pocket protein binding ... binds to the E2F1 transcription factor preventing it from interacting with the cell's transcription machinery. In the absence ...
  https://en.wikipedia.org/wiki/E2F
*  Transcriptional Regulation of Cyclin A2 by RASSF1A through the Enhanced Binding of p120E4F to the Cyclin A2 Promoter | Cancer...
pRB binds to and modulates the transrepressing activity of the E1A-regulated transcription factor p120E4F. Proc Natl Acad Sci U ... Interestingly, repression of the cyclin A2 promoter in quiescent cells was found to be associated with recruitment of the E2F-4 ... which did not compete with p120E4F binding. A similar EMSA profile is also seen in HeLa cells (lanes 8-11). EMSA results ( Fig ... DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation. Genes Dev 1989; 3: 620 ...
  http://cancerres.aacrjournals.org/content/65/7/2690
*  Similar articles for PubMed (Select 8446173) - PubMed - NCBI
E2F site activates transcription in fission yeast Schizosaccharomyces pombe and binds to a 30-kDa transcription factor. ... E2F-4, a new member of the E2F transcription factor family, interacts with p107. ... Regulation of adenovirus 12 E1A transcription: E2F and ATF motifs in the E1A promoter bind nuclear protein complexes including ... Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F. ...
  https://www.ncbi.nlm.nih.gov/pubmed?linkname=pubmed_pubmed&from_uid=8446173
*  Plus it
However, we previously provided the first in vivo evidence that a specific fragment of ER-α promoter is bound by pRb2/p130-E2F4 ... E2F4 and E2F5 transcription factors, and chromatin-modifying enzymes DNMT1, HDAC1, histone methyltransferase (SUV39H1), and ... which we found to be differentially associated with pRb2/p130 on the ER-α promoter, is similar in MDA-MB-231 and MCF-7 cells. ... In vivo binding of pRb1/p105, p107, and pRb2/p130 to ER-α promoter. We studied whether pRb1/p105 and p107 bind in vivo to ER-α ...
  http://cancerres.aacrjournals.org/content/67/16/7731
*  SMAD (protein) - Wikipedia
When there is no TGF-B present, a repressor complex composed of Smad3, and the transcription factors E2F4 and p107 exist in the ... where they can bind to DNA and regulate transcription. However, I-Smads are predominantly found in the nucleus, where they can ... of the Myc promoter to repress its transcription. In addition to Myc, Smads are also involved in the downregulation of ... A similar screen done in the Caenorhabditis elegans protein SMA (from gene sma for small body size) revealed three genes, Sma-2 ...
  https://en.wikipedia.org/wiki/SMAD_(protein)
*  Frontiers | Skin Tumors Rb(eing) Uncovered | Oncology
... unique and overlapping functions as master regulators of cell cycle progression and transcriptional modulation by similar ... unique and overlapping functions as master regulators of cell cycle progression and transcriptional modulation by similar ... p27Kip1 represses transcription by direct interaction with p130/E2F4 at the promoters of target genes. Oncogene (2012) 31(38): ... E2F1-E2F6 factors have a domain to bind DP and DNA, E2F1-5 have a pocket protein binding domain, and E2F7 and 8 can bind DNA ...
  https://www.frontiersin.org/articles/10.3389/fonc.2013.00307/full
*  Recombinant human SIRT1 protein (ab54334) | Abcam
Represses HNF1A-mediated transcription. Required for the repression of ESRRG by CREBZF. Modulates AP-1 transcription factor ... a promoter clearing mechanism after reach round of transcription is proposed. Involved in lipid metabolism. Implicated in ... Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Proposed to play role ... Appears to have a similar effect on MLLT7/FOXO4 in regulation of transcriptional activity and apoptosis. Deacetylates DNMT1; ...
  https://www.abcam.com/recombinant-human-sirt1-protein-ab54334.html
*  Plus it
1 Promoter. To identify the FLP/MYB88 binding sites in CDKB1;1, we cloned the 5′ region of CDKB1;1 from the transcription start ... and potentially other similar E2F factors, can recognize this element in vivo (Figures 7A and 7B). ... the human equivalent of the dREAM complex associates with the E2F4 repressor so that the complex represses cell cycle genes, ... FLP/MYB88 Binding Sites in the CDKB1;1 Promoter.. (A) Structure of the CDKB1;1 promoter showing the position of two putative ...
  http://www.plantcell.org/content/22/7/2306
*  WikiGenes - HCFC1 - host cell factor C1
The complete sequence of the host cell factor 1 (HCFC1) gene and its promoter: a role for YY1 transcription factor in the ... Luman and VP16 appear to have similar mechanisms for binding HCF, as in vitro each competitively inhibited the binding of the ... Host Cell Factor-1 and E2F4 Interact Via Multiple Determinants in Each Protein. Knez, J., Piluso, D., Bilan, P., Capone, J.P. ... Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman. Misra, V., Rapin, N., ...
  http://www.wikigenes.org/e/gene/e/3054.html
*  QSER1 - Wikipedia
Predicted transcription factors with conservation include (but are not limited to) EGR1, p53, E2F3, E2F4, PLAG1, NeuroD2, Myf5 ... The structurally similar region is on the exterior of the protein molecule and forms part of the DNA binding cleft. Further ... The promoter region for QSER1 is 683 bp in length and is found on chromosome 11 between 32,914,224 bp and 32,914,906. There is ... An image of the structurally similar region and sequence alignment is shown on the right. Regions before the identified ...
  https://en.wikipedia.org/wiki/QSER1
*  Oncogene - Biological reprogramming in acquired resistance to endocrine therapy of breast cancer
... the representation of predicted transcription factor binding sites (TFBSs) at promoters was evaluated using predictions from ... Balciunaite et al., 2005) showed similar results for E2F4 response elements (underexpressed set, infra-representation P=0.072; ... 2008). JASPAR, the open access database of transcription factor-binding profiles: new content and tools in the 2008 update. ... Figure 4a, bottom panels), a transcription factor involved in stem cell self-renewal (. Galan-Caridad et al., 2007). The ...
  http://www.nature.com/onc/journal/v29/n45/full/onc2010333a.html?foxtrotcallback=true&error=cookies_not_supported&code=4e4de2b9-362c-43f6-b1eb-2244ff9569fc
*  Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
Through its ability to bind the E2F transcription factors, the RB family of proteins (RB, p107, and p130) plays a major role in ... as well as a switch between the E2F3 activator and the E2F4 repressor at the Foxn1 promoter (Fig. 4 I). Collectively, these ... However, we found that the expression of Eya1, Pax1, Pax9, Six1, Hoxa3, and Tbx1 was similar in control and mutant TECs at 3 mo ... We found that E2F3 and E2F4, a repressor E2F, can bind to both site 1 and site 2-3 but do not bind significantly to regions of ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3674705/?lang=en-ca
*  Protocols and Video Articles Authored by Patrick Collins
Transcription Factor Binding and Modified Histones in Human Bidirectional Promoters Genome Research. Jun, 2007 , Pubmed ID: ... We found that a small set of motifs, including GABPA, MYC, E2F1, E2F4, NRF-1, CCAAT, YY1, and ACTACAnnTCC are overrepresented ... Five samples showed levels of resistance similar to the reference strong-resistance strain. A representative highly resistant ... including the ubiquitous ets-family transcription factor GA-binding protein (GABP). Here we show that GABP binds to more than ...
  https://www.jove.com/author/Patrick_Collins
*  EEF1A1 | Cancer Genetics Web
Mechanistically, E2F-4 binds constitutively to the TP53 gene and induces transcription. TGF-β1-activated Smads are recruited to ... regulation of transcription, DNA-dependent - transcription, DNA-dependent - translation - translation elongation factor ... Similar results were obtained with mHLA-G(EF1α)-hESC-derived epidermal progenitors (hEEP). One way mixed T lymphocyte reactions ... The XPG-TFIIH complex was recruited to promoter and coding regions of both EGF-induced (FOS) and housekeeping (EEF1A1) genes. ...
  http://www.cancerindex.org/geneweb/EEF1A1.htm
*  Retroviral vectors for establishing tetracycline-regulated gene expression in an otherwise recalcitrant cell line | BMC...
... the elongation factor 1-alpha promoter (EF1α) or the phosphoglycerate kinase-1 promoter (PGK), and compared the resulting cell ... In contrast to cells produced using the CMV and PGK promoters, those produced using the EF1α and TRE promoters expressed high ... We generated retroviruses in which tTA expression was controlled by one of three promoters: a synthetic tetracycline responsive ... lines to one generated using a cytomegalovirus immediate early gene promoter (CMV). ...
  https://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-3-13
*  Frontiers | "Till Death Do Us Part": A Potential Irreversible Link Between Aberrant Cell Cycle Control and Neurodegeneration in...
SVZ and OE neurogenesis is associated with compensatory pro-survival mechanisms in the aging OB which are interestingly similar ... In the SVZ, such regulatory factors include mitogens, growth factors, transcription factors, chromatin modifiers and non-coding ... Similar Pro-Survival Mechanisms Are Detected During Aberrant OB Neurogenesis and Neurodegenerative Diseases. The OB has been ... One way to resolve this issue is to generate inducible conditional KO-reporter mice under the control of specific promoters and ...
  https://www.frontiersin.org/articles/10.3389/fnins.2018.00144/full
*  Gavin Sherlock | Stanford Medicine Profiles
Analysis of ChIP-seq data from a panel of cell cycle transcription factors (E2F1, E2F4, E2F6, and GABPA) from the Encyclopedia ... We identify genomic loci bound by the G2/M transcription factor FOXM1 by chromatin immunoprecipitation followed by high- ... Resultant tumors had a phenotypic diversity similar to that of the original tumor and behaved in a similar manner when passaged ... Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known ...
  https://med.stanford.edu/profiles/gavin-sherlock
*  Meta-Analysis of Differential Connectivity in Gene Co-Expression Networks in Multiple Sclerosis
In detail, we searched for the transcription factor binding sites (TFBSs) in the promoter regions and microRNA binding motifs ... the most significant transcription factors potentially dysregulated in MS are YY1 (yin and yang 1), E2F-1/DP-1 and E2F-4/DP-2 ... MS and normal networks were characterized by similar topological behaviors (Figure 2): both of them were composed of nodes with ... Overrepresented Transcription Factors Binding Sites. Many of the transcription factor binding sites (TFBSs) enriched in both ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC4926469/
*  E2F1-3 Switch from Activators in Progenitor Cells to Repressors in Differentiating Cells
E2Fs function as transcription factors, with E2F1-3 as activators and E2F4-8 as repressors3-8. Although it is a maxim of ... The binding of E2F4 (b) and p130 (c) to E2F target promoters in control (−) or cre-treated (+) E2f1−/−;E2f2−/−;E2f3LoxP/LoxP ... We utilized an unbiased method similar to Gene Set Enrichment Analysis to identify genes that were differentially expressed16. ... A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F. Cell. 1992;70:337-350. [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2806193/
*  Activation of Cdc6 by MyoD is associated with the expansion of quiescent myogenic satellite cells | JCB
... was found to be the most predominant factor in association with this promoter, although a weak binding by E2F4 could also be ... The E2F1-3 transcription factors are essential for cellular proliferation. Nature. 414:457-462. doi:10.1038/35106593. ... 1 A). The arrangement of these two E2F elements is quite similar to that found in the human Cdc6 promoter (Williams et al., ... Precipitated DNA was analyzed for the presence of the Cdc6 promoter by PCR. (D) The occupation of the Cdc6 promoter by E2F4 ...
  http://jcb.rupress.org/content/188/1/39
*  Hypoxia suffocates histone demethylases to change gene expression: a metabolic control of histone methylation
First, hypoxia activates transcription factors (TF) such as Hypoxia-inducible Factor (HIF). Second, hypoxia decreases the ... TFs showing similar binding strengths to their target genes might be under the 'metabolic control' which changes histone ... promoter, enhancer, coding region, transcription start site (TSS), intergenic region), the combination of histone modifications ... Among the 75 TFs, we performed the ChIP assays for the positive target genes of the six TFs (JUN, ARNT, CREB1, HIF1A, E2F4, and ...
  http://www.bmbreports.org/journal/view.html?doi=10.5483/BMBRep.2017.50.11.200
*  Oxidative status of muscle is determined by p107 regulation of PGC-1α | JCB
... are recruited to promoters to repress transcription through binding to DNA-binding transcription factors such as E2F1 and E2F4 ... 1 D). In addition, 2-yr-old p107−/− mice did not exhibit any significant weight gain and were similar in weight to 3-mo-old ... Chromatin immunoprecipitation revealed binding of p107 and E2F4 to the PGC-1α proximal promoter, and this binding repressed ... the PGC-1α promoter appears to be modulated directly by p107 interacting with bound transcription factors that are responsive ...
  http://jcb.rupress.org/content/190/4/651
*  Post‐transcriptional gene expression control by NANOS is up‐regulated and functionally important in pRb‐deficient cells | The...
E2F4, p107, and p130, confirmed that all three proteins bind directly to the promoter of NANOS1 (NOS1) in human fibroblasts (BJ ... regulation of the E2F transcription factors would affect these putative PUM substrates, we analyzed the promoters of these ... Our data suggest that upon pRb‐inactivation cells stimulate the expression of NANOS1 to similar levels with both PUM1 and PUM2 ... pGl4 (Promega), pGL3 (Promega), pGL3‐pum promoter region, pGL3‐nanos promoter region, pGL3‐brat promoter region, psi‐CHECK2 ( ...
  http://emboj.embopress.org/content/early/2014/08/06/embj.201488057
*  Modeling Gene Regulation in Liver Hepatocellular Carcinoma with Random Forests
Mapping Binding Sites of Transcription Factors and MicroRNAs. We defined the promoter regions as the 2000 nt region upstream or ... We observed a similar result for the TF ZEB1. A previous study showed that ZEB1 suppresses the expression of CDH13 (T-cadherin ... X. Xu, B. Mark, V. X. Jin et al., "A comprehensive ChIP-chip analysis of E2F1, E2F4, and E2F6 in normal and tumor cells reveals ... Transcriptional regulation is mainly controlled by binding of transcription factors (TFs) to gene promoters to control ...
  https://www.hindawi.com/journals/bmri/2016/1035945/