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*  Alison Weiss, PhD - ..WB1PRD02W-med.uc.edu
Comparison of binding platforms yields insights into receptor binding differences between shiga toxins 1 and 2. Biochemistry, ... Binding of Pk-trisaccharide analogs of Globotriaosylceramide to Shiga toxin variants. Infect. Immun, 81 , ... Glycolipid Binding Preferences of Shiga Toxin Variants PLoS ONE, 9(7): e101173 , ... Shiga toxin binding to glycolipids and glycans. PloS one, 7 2, e30368 ...
  https://med.uc.edu/molgen/molgen-directory/faculty-directory/Index/Pubs/weissaa/
*  Relative Nephroprotection During Escherichia coli O157:H7 Infections: Association With Intravenous Volume Expansion |...
Evidence that verotoxins (Shiga-like toxins) from Escherichia coli bind to P blood group antigens of human erythrocytes in ... Differentiation-associated toxin receptor modulation, cytokine production, and sensitivity to Shiga-like toxins in human ... erythrocytes do not bind Shiga toxin that is produced by E coli O157:H7 at body temperature38; (2) antigens that are expressed ... Cytotoxic effect of Shiga toxin-1 on human glomerular epithelial cells. Kidney Int.2000;57 :2350- 2359. ...
  http://pediatrics.aappublications.org/content/115/6/e673
*  ASMscience | New Therapeutic Developm
These toxins are briefly described in the section on therapeutics targeted to the Stxs. ... Shiga toxin (Stx)-producing Escherichia coli (STEC) colonizes the intestine and causes hemorrhagic colitis. STEC encodes a ... Role of Shiga toxin 2 (Stx2)-binding protein, human serum amyloid P component (HuSAP), in Shiga toxin-producing Escherichia ... Shiga toxin-binding site for host cell receptor GPP130 reveals unexpected divergence in toxin-trafficking mechanisms. Mol Biol ...
  http://www.asmscience.org/content/book/10.1128/9781555818791.chap17
*  Thrombotic microangiopathy - Wikipedia
The Shiga-toxin inhibits the binding of eEF-1-dependent binding of aminoacyl tRNA to the 60S subunit of the ribosome, thus ... HUS can be divided into two main categories: Shiga-toxin-associated HUS (STx-HUS), which normally presents with diarrhea, and ... Generally, renal complications are particularly predominant with Shiga-toxin-associated hemolytic uremic syndrome (STx-HUS) and ... bacterial Shiga toxins or endotoxins, antibodies, and drugs; and congenital predisposing conditions, including decreased levels ...
  https://en.wikipedia.org/wiki/Thrombotic_microangiopathy
*  WikiGenes - B3GALNT1 - beta-1,3-N-acetylgalactosaminyltransferase...
Kinetic analysis of binding between Shiga toxin and receptor glycolipid Gb3Cer by surface plasmon resonance. Nakajima, H., ... Isolation of a shigella toxin-binding glycolipid from rabbit jejunum and HeLa cells and its identification as ... In addition, toxin bound to P1 antigen present in group B human erythrocyte glycolipid extracts [7]. ... In addition, this assay was shown to be specific, because neither Stx1 nor Stx2 bound to GM3, but both bound weakly to Gb4Cer [ ...
  https://www.wikigenes.org/e/gene/e/8706.html
*  Toxins | Free Full-Text | Impact of the Nature and Size of the Polymeric Backbone on the Ability of Heterobifunctional Ligands...
Effective in vivo protection from Shiga toxin Type 1 (Stx1) is achieved by polymer-bound, heterobifunctional inhibitors- ... that mediate assembly of supramolecular complexes involving the toxin's pentameric cell membrane-binding subunit and an ... the cytotoxicity assay and in vivo indicate that the mechanism of protection from intoxication is inhibition of toxin binding ... The polymeric scaffold influences the activity not only by clustering active binding fragments but also by sterically ...
  http://www.mdpi.com/2072-6651/3/9/1065/htm
*  Identification of TLR4 as the Receptor That Recognizes Shiga Toxins in Human Neutrophils | The Journal of Immunology
Shiga toxin activates complement and binds factor H: evidence for an active role of complement in hemolytic uremic syndrome. J ... Shiga toxin-producing Escherichia coli. Stx. Shiga toxin. Stx1. Shiga toxin 1. Stx2. Shiga toxin 2.. ... The interactions of human neutrophils with shiga toxins and related plant toxins: danger or safety? Toxins (Basel) 4: 157-190. ... Differential binding of Shiga toxin 2 to human and murine neutrophils. J. Med. Microbiol. 56: 1423-1430. ...
  http://www.jimmunol.org/content/191/9/4748
*  Shiga-like toxin - Wikipedia
The toxin acts on the lining of the blood vessels, the vascular endothelium. The B subunits of the toxin bind to a component of ... 2017), "Chapter 3: Structure of Shiga toxins and other AB5 toxins", Shiga toxins: A Review of Structure, Mechanism, and ... "versions of the same toxin" rather than "different toxins." As with Shiga toxin, the toxin requires highly specific receptors ... Shiga-Like Toxin I at the US National Library of Medicine Medical Subject Headings (MeSH) Shiga-Like Toxin II at the US ...
  https://en.wikipedia.org/wiki/Shiga-like_toxin
*  M- Bacterial Infections of the Gut Flashcards by heather Fahey | Brainscape
2. Phage-encoded Shiga toxin binds to G3 glycolipid receptor on epithelial cells and cause secretion. It is also renal toxic by ... ETEC has a heat labile (LT) and heat stabile (ST) toxin. LT is an ab toxin like cholera. B binds to GM1 ganglioside receptor ... 3. Secrete cholera toxin (A-B with one A and five B) 4. the five B form a pentamer and bind to GM1 ganglioside. 5. the A ... 3. Secrete cholera toxin (A-B with one A and five B) 4. the five B form a pentamer and bind to GM1 ganglioside. 5. the A ...
  https://www.brainscape.com/flashcards/m-bacterial-infections-of-the-gut-1739836/packs/3239092
*  Interaction of the Shiga-like toxin type 1 B-subunit with its carbohydrate receptor. | Scholars@Duke
Interaction of the Shiga-like toxin type 1 B-subunit with its carbohydrate receptor.'s profile, publications, research topics, ... A study of the binding of the Shiga-like toxin 1 (SLT-1) to the P(k) trisaccharide [methyl 4-O-(4-O-alpha-D-galactopyranosyl)-4 ... Interaction of the Shiga-like toxin type 1 B-subunit with its carbohydrate receptor. Journal Article ... On the basis of the thermodynamics of binding, optical spectroscopy, and binding-induced aggregation, we propose a model of SLT ...
  https://scholars.duke.edu/display/pub649461
*  Frontiers | Simulation Study of cDNA Dataset to Investigate Possible Association of Differentially Expressed Genes of Human...
2 and NFKB1 transcription factor binding sites (TFBS) involved in the complex biological functions. Thus, our findings suggest ... Shiga toxin (Stxs) is a family of structurally and functionally related bacterial cytotoxins produced by Shigella dysenteriae ... Shiga toxin (Stxs) is a family of structurally and functionally related bacterial cytotoxins produced by Shigella dysenteriae ... The functional enrichment analysis showed 6 down-regulated and 1 up-regulated genes. Among these DEGs, IL6 was associated with ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2018.00380/full
*  Mallory-Weiss Tear and Pulmonary Mets | UCSF Internal Medicine Chief Resident Hub
1) Case 1 was of a young man who presented with an acute onset of emesis with progression to bloody emesis, melena, and ... Oral Shiga toxin-binding agent. *Eculizumab: Monoclonal antibody to C5 complement factor blocking complement activation. There ... Shiga toxin released by E. coli binds to globotriaosylceramide (Gb3) on surface of vascular endothelial cells, particularly in ... Shiga toxin also activates alternative pathway of complement system by binding to factor H proteins! ...
  https://ucsfmed.wordpress.com/2017/02/06/mallory-weiss-tear-and-pulmonary-mets/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... the receptor-binding B-subunit of Shiga toxin (STxB). RME-8 has previously been found to bind to the clathrin uncoating ... one of which is the well-explored bacterial Shiga toxin. ADP-ribosylation factors (Arfs) are approximately 20 kDa GTP-binding ... cholera toxin and the endogenous proteins TGN46 and mannose 6-phosphate receptor. In AGAP2-depleted cells, Shiga toxin ... Previous studies on Shiga toxin suggested that these two coat complexes operate in a sequential manner. Here, we show that the ...
  https://www.jove.com/visualize?author=Patricia+V+Burgos
*  С ?? ҩ ??.??Ϣ?? .11254001??11254500
11254352:Studies of Fibronectin-Binding Proteins of Streptococcus equi. *11254353:Shiga Toxin 1 Causes Direct Renal Injury in ... 11254004:Shiga Toxin 1 Induces Apoptosis in the Human Myelogenous Leukemia Cell Line THP-1 by a Caspase-8-Dependent, Tumor ... 11254334:Recombinant Shiga Toxin B-Subunit-Keyhole Limpet Hemocyanin Conjugate Vaccine Protects Mice from Shigatoxemia ... 11254008:Mutation of the Zinc-Binding Metalloprotease Motif Affects Bacteroides fragilis Toxin Activity but Does Not Affect ...
  http://www.100md.com/index/other/byinfono/022/509.htm
*  Toxins | Free Full-Text | Verotoxin A Subunit Protects Lymphocytes and T Cell Lines against X4 HIV Infection in Vitro
Gb3 is the sole receptor for Escherichia coli verotoxins (VTs, Shiga toxins). VT1 contains a ribosome inactivating A subunit ( ... VT1 showed no binding or obvious Jurkat cell/PBMC effect. Protective VT1 concentrations reduced PBMC (but not Jurkat cell) ... VT1A) non-covalently associated with five smaller receptor-binding B subunits. The effect of VT on PHA/IL2-activated PBMC HIV ... Gb3 is the sole receptor for Escherichia coli verotoxins (VTs, Shiga toxins). VT1 contains a ribosome inactivating A subunit ( ...
  http://www.mdpi.com/2072-6651/4/12/1517
*  Caveolae/raft-dependent endocytosis | JCB
For instance, cholera, shiga, and anthrax toxins bind to cell surface raft domains and yet are internalized via clathrin- ... Endocytosis from coated pits of Shiga toxin: a glycolipid-binding protein from Shigella dysenteriae 1. J. Cell Biol. 108:1331- ... Caveolae and raft pathways mediate the internalization of sphingolipids and sphingolipid binding toxins (cholera toxin [CTX]* ... Filipin-dependent inhibition of cholera toxin: evidence for toxin internalization and activation through caveolae-like domains ...
  http://jcb.rupress.org/content/161/4/673
*  Renal Involvement in Children with HUS | SpringerLink
Effect of an oral Shiga toxin binding agent on diarrhea-associated hemolytic uremic syndrome in children: a randomized control ... consisting of the B subunit of shiga toxin type 2 and brucella lumazine synthase confers total protection against Shiga toxins ... A single VHH-based toxin neutralizing agent and an effector antibody protect mice against challenge with Shiga toxin 1 and 2. ... Epidemic profile of Shiga toxin producing Escherichia coli O104:H4 outbreak in Germany. N Engl J Med. 2011;365:1771-80.PubMed ...
  https://link.springer.com/referenceworkentry/10.1007/978-3-642-27843-3_43-1
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Shiga toxin binding to glycolipids and glycans. Karen M Gallegos, Deborah G Conrady, Sayali S Karve, Thusitha S Gunasekera, ... most internalized toxin is routed to the lysosomes for degradation, so only a small fraction of surface-bound toxin reaches the ... AB toxins consist of an enzymatic A subunit and a cell-binding B subunit1. These toxins are secreted into the extracellular ... Immunologically distinct forms of Shiga toxin (Stx1 and Stx2) display different potencies and disease outcomes, likely due to ...
  https://www.jove.com/visualize/abstract/22348006/shiga-toxin-binding-to-glycolipids-and-glycans
*  Thrombotic thrombocytopenic purpura
Caused by Shiga toxin-producing bacteria (for example, Escherichia coli O157:H7, diarrhea-associated HUS) or defective control ... These are anti-angiogenesis agents that block VEGF binding to, and signalling between, glomerular endothelial and other renal ... Review of TTP and Shiga toxin-related HUS with excellent illustrations.) Zheng, XL, Sadler, JE. "Pathogenesis of thrombotic ... Shiga toxins, and high concentrations of histamine. The spacer and CUB-1 domains of ADAMTS-13 bind ADAMTS-13 to the ULVWF ...
  https://www.psychiatryadvisor.com/hematology/thrombotic-thrombocytopenic-purpura/article/597453/
*  Ability of Manganese in Neutralizing Fatal Shiga Toxin, Reveals Carnegie Mellon Study
An element usually found in nature may give way to neutralize the potentially toxic effects of a compound known as Shiga toxin ... Shiga toxin binds to GPP130, hitching a ride on a route that doesn't go to the lysosome. Instead, the toxin is carried to the ... "I knew that Shiga toxin was one of the key cargo molecules that bypass the lysosome as they go from the endosome to the Golgi ... Produced by certain bacteria, including Shigella and some strains of E. coli, Shiga toxin can cause symptoms ranging from mild ...
  http://www.medindia.net/news/Ability-of-Manganese-in-Neutralizing-Fatal-Shiga-Toxin-Reveals-Carnegie-Mellon-Study-96485-1.htm
*  David I. Friedman, M.D. | Microbiology & Immunology | Michigan Medicine | University of Michigan
The operator and early promoter region of the Shiga toxin type 2-encoding bacteriophage 933W and control of toxin expression. J ... Zhou, Y. Mah, T.-F., Greenblatt, J., Friedman, D.I. (2002) Evidence that the KH RNA-Binding Domains Influence the Action of the ... Shiga-like toxin: One serious public health problem that is of current concern is the infections caused by certain Escherichia ... The major contributor to the virulence of these bacteria are the products of genes encoding Shiga toxin (Stx), a glucosidase ...
  https://medicine.umich.edu/dept/microbiology-immunology/david-i-friedman-md
*  Thrombotic Microangiopathy | SpringerLink
O'Brien AD, Holmes RK: Shiga and Shiga-like toxins. Microbiol Rev 51: 206-220, 1987.PubMedGoogle Scholar ... Lingwood CA, Law H, Richardson S, Petric M, Brunton JL, De Grandis S, Karmali M: Glycolipid binding of purified and recombinant ... Identification of the carbohydrate receptor for Shiga toxin produced by Shigella dysenteriae type 1. J Biol Chem 262: 1779-1785 ... I. (Shiga) like cytotoxin. Lancet 1: 702, 1983.PubMedCrossRefGoogle Scholar ...
  https://link.springer.com/chapter/10.1007/978-1-4757-6632-5_31
*  Carnegie Mellon study reveals potential of manganese in neutralizing deadly S... ( PITTSBURGHCarnegie Mellon University...)
After entering the body Shiga toxin is secreted by the infecting bact...,Carnegie,Mellon,study,reveals,potential,of,manganese, ... in,neutralizing,deadly,Shiga,toxin,biological,advanced biology technology,biology laboratory technology,biology device ... Shiga toxin binds to GPP130, hitching a ride on a route that doesn't go to the lysosome. Instead, the toxin is carried to the ... "I knew that Shiga toxin was one of the key cargo molecules that bypass the lysosome as they go from the endosome to the Golgi ...
  http://www.bio-medicine.org/biology-technology-1/Carnegie-Mellon-study-reveals-potential-of-manganese-in-neutralizing-deadly-Shiga-toxin-21560-1/
*  Aspergillus ustus - Wikipedia
"Functional analysis of the Shiga toxin and Shiga-like toxin type II variant binding subunits by using site-directed mutagenesis ... 48 (1): 53-66. Kohno, J; Sakurai, M; Kameda, N; Nishio, M; Kawano, K; Kishi, N; Okuda, T; Komatsubara, S (October 1999). " ... 2013: 1-18. doi:10.1155/2013/260395. PMC 3665174 . PMID 23762830. Samson, R. A.; Varga, J.; Meijer, M.; Frisvad, J. C. (8 ... 69 (1): 81-97. doi:10.3114/sim.2011.69.06. Kane, Julius (1997). Laboratory Handbook of Dermatophytes: A Clinical Guide and ...
  https://en.wikipedia.org/wiki/Aspergillus_ustus