ZO-3, a Novel Member of the MAGUK Protein Family Found at the Tight Junction, Interacts with ZO-1 and Occludin | JCB
Mutations in the GUK domain of Drosophila dlg-A result in loss of normal epithelial cell polarity and neoplastic overgrowth in ... between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. The ... Cell Culture. MDCK strain II cells were grown and metabolically labeled as described previously (2, 37). The MDCK cell line ... This construct was then transfected into MDCK cells to generate the stable MDCK/Z3 cell line. Immunoblot of a whole cell lysate ...http://jcb.rupress.org/content/141/1/199
Regulation of F-actin-dependent processes by the Abl family of tyrosine kinases | Journal of Cell Science
Small, J. V., Isenberg, G. and Celis, J. E. (1978). Polarity of actin at the leading edge of cultured cells. Nature 272,638 - ... The actin latticework at the apical surface is also disrupted and ectopic actin-rich deposits occur at the basolateral surface ... Rate of cell migration is reduced by c-Abl. Random cell migration towards fibronectin is also affected by c-Abl activity, ... These cultured-cell-based results are intriguing but future work will be required to determine whether cell migration and DNA ...http://jcs.biologists.org/content/116/13/2613
The in vivo function of mammalian cell and tissue polarity regulators - how to shape and maintain the epidermal barrier |...
Apico-basolateral polarity in a stratifying epithelium. Simple epithelial cells polarize to establish two different membrane ... Polarity comes in two main forms: cell polarity and tissue polarity. In cell polarity, asymmetry is achieved in individual ... 2008). Cell polarity and cancer-cell and tissue polarity as a non-canonical tumor suppressor. J. Cell Sci. 121, 1141-1150. doi: ... 2010). Cell polarity in eggs and epithelia: parallels and diversity. Cell 141, 757-774. doi:10.1016/j.cell.2010.05.011. ...http://jcs.biologists.org/content/125/15/3501
VIPAS39 (VPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog)
... apical-basolateral polarity regulator, spe-39 homolog), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol. ... intracellular protein transport spermatogenesis endosome to lysosome transport endosome to lysosome transport cell ... VIPAS39 63894 VPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog. ... intracellular protein transport spermatogenesis endosome to lysosome transport endosome to lysosome transport cell ...http://atlasgeneticsoncology.org/Genes/GC_VIPAS39.html
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cellular Biology, Issue 51, Epithelial cells, cell polarity, microinjection, basolateral sorting, MDCK ... 4D live cell imaging, live cells, laser, cell biology, protein folding, Ubc9ts, yeast, assay, cell, imaging ... Subsequently, the infected cells are seeded on coverslips to finally enable live cell imaging. Then, living cells are incubated ... Apical-basolateral polarization can be recapitulated successfully in culture when epithelial cells such as Madin-Darby Canine ...https://www.jove.com/visualize/abstract/24594942/the-chaperone-bag6-captures-dislocated-glycoproteins-in-the-cytosol
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cellular Biology, Issue 51, Epithelial cells, cell polarity, microinjection, basolateral sorting, MDCK ... The cell suspension is then passed through a 40 um nylon cell strainer. Next the cells are counted. The neurons are now ready ... Phagocytosis is a process in which a cell engulfs material (entire cell, parts of a cell, debris, etc.) in its surrounding ... Apical-basolateral polarization can be recapitulated successfully in culture when epithelial cells such as Madin-Darby Canine ...https://www.jove.com/visualize/abstract/24348940/correction-insight-into-molecular-functional-properties-nmnat3
Mir-451 microRNA - Wikipedia
"MicroRNA-338-3p and microRNA-451 contribute to the formation of basolateral polarity in epithelial cells". Nucleic Acids Res. ... "MicroRNA-451 regulates LKB1/AMPK signaling and allows adaptation to metabolic stress in glioma cells". Mol Cell. 37 (5): 620-32 ... Godlewski J, Bronisz A, Nowicki MO, Chiocca EA, Lawler S (2010). "microRNA-451: A conditional switch controlling glioma cell ... Bruchova-Votavova H, Yoon D, Prchal JT (2010). "miR-451 enhances erythroid differentiation in K562 cells". Leuk Lymphoma. 51 (4 ...https://en.wikipedia.org/wiki/Mir-451_microRNA
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cellular Biology, Issue 51, Epithelial cells, cell polarity, microinjection, basolateral sorting, MDCK ... The cell suspension is then passed through a 40 um nylon cell strainer. Next the cells are counted. The neurons are now ready ... Phagocytosis is a process in which a cell engulfs material (entire cell, parts of a cell, debris, etc.) in its surrounding ... Huh-7.5 cells). Subsequently, these cells were co-cultured and fused to various human and murine cells expressing HCV ...https://www.jove.com/visualize/abstract/24348978/correction-fluvastatin-interferes-with-hepatitis-c-virus-replication
Helicobacter pylori perturbs iron trafficking in the epithelium to grow on the cell surface.
... perturbs cell-cell junctions, and alters host cell polarity. This enables Hp to grow as microcolonies adhered to the host ... injects the CagA effector protein into host epithelial cells and induces growth factor-like signaling, ... Year: 1996Apical and basolateral endosomes of MDCK cells are interconnected and contain a polarized sorting mechanism.J Cell ... Caco-2 Cells. Cell Line. Cell Membrane / metabolism, microbiology. Cell Polarity / physiology. Dogs. Down-Regulation. ...http://www.biomedsearch.com/nih/Helicobacter-pylori-Perturbs-Iron-Trafficking/21589900.html
Most recent papers with the keyword C terminal Src kinase CSK in cancer | Read by QxMD
Most notably this toxin is involved in the disruption of apical-basolateral cell polarity and cell adhesion, as well as in the ... The expression of Cbp is down-regulated in Src-transformed cells and in various human cancer cells, suggesting a potential role ... In this study, we found that Cbp was markedly downregulated in human non-small cell lung cancer (NSCLC) cells... ... Following host cell translocation, CagA interferes with various host-cell signalling pathways. ...https://www.readbyqxmd.com/keyword/82766
Apical-to-Basolateral Transcytosis of Photoreceptor Outer Segments Induced by Lipid Peroxidation Products in Human Retinal...
Use of the ARPE-19 cell line as a model of RPE polarity: basolateral secretion of FGF5. Invest Ophthalmol Vis Sci. 1998; 39(13 ... Retinal Cell Biology , January 2010. Apical-to-Basolateral Transcytosis of Photoreceptor Outer Segments Induced by Lipid ... In the present study, we provide evidence that human RPE cells in vitro use apical-to-basolateral transcytosis as a means of ... The predominant fraction of undegraded POS proteins, however, was detected in the medium on the basolateral side of the cells, ...https://iovs.arvojournals.org/article.aspx?articleid=2186401
Shape meets polarity in Japan | Development
Dlg and Lgl are normally localized to basolateral cell-cell junctions and regulate apicobasal polarity. They also control cell ... T cell polarity. Activation of naïve cells by interaction with a cognate antigen-presenting cell can lead to asymmetric cell ... Polarity systems that listen to neighboring cells. It is becoming increasingly clear that Wnt signaling and other cell-cell ... a single cell undergoes asymmetric cell division, and its daughter cells will adopt diverse cell fates (Chang et al., 2007). ...http://dev.biologists.org/content/136/15/2487
Leptin promotes epithelial-mesenchymal transition of breast cancer via the upregulation of pyruvate kinase M2 | Journal of...
... cancer cells undergo morphological changes, such as cell-cell junction dissolution, loss of apical-basolateral cell polarity, ... these cell morphological changes such as cell-cell junction dissolution and loss of apical-basolateral cell polarity were ... The image showed cell-cell junction dissolution and loss of apical-basolateral cell polarity in MCF-7, SK-BR-3, and MDA-MB-468 ... Cell culture. The human breast cancer cell lines MCF-7, SK-BR-3 and MDA-MB-468 were obtained from American Type Culture ...https://jeccr.biomedcentral.com/articles/10.1186/s13046-016-0446-4
Role of membrane traffic in the generation of epithelial cell asymmetry.
Epithelial cells have an apical-basolateral axis of polarity, which is required for epithelial functions including barrier ... Epithelial cells have an apical-basolateral axis of polarity, which is required for epithelial functions including barrier ... Cell Membrane / metabolism*. Cell Polarity / genetics, physiology. Epithelial Cells / cytology*, metabolism*. Humans. Mice. ... Title: Nature cell biology Volume: 14 ISSN: 1476-4679 ISO Abbreviation: Nat. Cell Biol. Publication Date: 2012 Dec ...http://www.biomedsearch.com/nih/Role-membrane-traffic-in-generation/23196841.html
Redistribution of Tight Junction Proteins During EPEC Infection In Vivo | Springer for Research & Development
Disruption of cell polarity by enteropathogenic Escherichia coli enables basolateral membrane proteins to migrate apically and ... Exploitation of host cells by enteropathogenic Escherichia coli. Proceedings of the National Academy of Sciences of the United ... The effector repertoire of enteropathogenic E. coli: ganging up on the host cell. Current Opinion in Microbiology 12: 101-109. ... Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions. The FEBS Journal 275: 6022- ...https://rd.springer.com/article/10.1007/s10753-010-9285-1
LIN7A is a major determinant of cell-polarity defects in breast carcinomas | Breast Cancer Research | Full Text
Cells from invasive micropapillary carcinomas (IMPCs) of the breast are characterized by a striking cell polarity inversion and ... revealing strong polarity defects. This study therefore shows that LIN7A has a crucial role in the polarity abnormalities ... LIN7A, a Crumbs-complex polarity gene, was one of the most differentially over-expressed genes in the IMPCs. Upon LIN7A over- ... In-depth investigation of polarity proteins in 24 IMPCs and a gene expression profiling, comparing IMPC (n = 73) with invasive ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-016-0680-x
Interaction between Erbin and a Catenin-related protein in epithelial cells.
Integrity of epithelial tissues relies on the proper apical-basolateral polarity of epithelial cells. Members of the LAP (LRR ... Erbin and ERBB2/HER2 are located in the basolateral membranes of epithelial cells. We show here that Erbin interacts with p0071 ... protein family such as LET-413 and Scribble are involved in maintaining epithelial cell polarity in Caenorhabditis elegans and ... Interaction between Erbin and a Catenin-related protein in epithelial cells.. *. Fanny Jaulin-Bastard, J. P. Arsanto, André le ...http://1uj.pertussis.us/paper/74k5j
Angiomotin - Wikipedia
In the outer cells, the cell polarity sequesters AMOT from basolateral adherens junctions to apical domains, thereby ... Angiomotin p130 does not promote cell migration, nor responds to angiostatin. It localizes to cell-cell junction like p80 and ... Transfection of p130 angiomotin into MAE cells results in change in cell shape, increased average cell size and stress fiber ... "A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells". Cell. 125 (3): 535-48. doi: ...https://en.wikipedia.org/wiki/Angiomotin
BMN673 - BET-bromodomain inhibition research
... go through morphological changes such as for example cell-cell junction dissolution lack of apical-basolateral cell polarity ... Strategies Cell lifestyle The human breasts cancer tumor cell lines MCF-7 SK-BR-3 and MDA-MB-468 had been extracted from ... Immunofluorescence evaluation MCF-7 MDA-MB-468 and SK-BR-3 cells were grown on coverslips. Cells were cleaned with PBS set with ... All cells were incubated in 4 right away?°C using the corresponding primary antibodies(OBR mouse. ...http://healthandwellnesssource.org/tag/bmn673/
Extracellular Matrix & Adhesion Molecules Arrays and Assays
In addition, epithelial cells have an apical-basolateral polarity and attach to basement membrane. ... Cell adhesion is the binding of the cells to each other and to the extracellular matrix through cell adhesion molecules (CAMs) ... Adherens junctions are cell-to-cell junctions between epithelial cells and are composed of cadherins and alpha-, beta-, and ... In other words, mesenchymal cells can arise from epithelial cells. During this process, epithelial cells will lose many of ...http://www.sabiosciences.com/ECM_CellAdhesion.php
Activation of apical chloride channels in the gastric oxyntic cell | Science
Oxyntic cells that retain distinct morphological polarity between apical and basolateral membranes were isolated from the ... oxyntic cells. Stimulation of the cells with dibutyryladenosine 3',5'-monophosphate and isobutylmethylxanthine increased ... Activation of apical chloride channels in the gastric oxyntic cell Message Subject. (Your Name) has forwarded a page to you ... Patch-clamp techniques were applied to these cells to identify apical membrane ion channels associated with hydrochloric acid ...http://science.sciencemag.org/content/245/4916/402
Symmetric and asymmetric mitotic segregation patterns influence Wolbachia distribution in host somatic tissue | Journal of Cell...
... epithelial cells have a distinct cortical polarity, consisting of apical and basolateral domains (Hutterer et al., 2004; Suzuki ... Wodarz, A. (2005). Molecular control of cell polarity and asymmetric cell division in Drosophila neuroblasts. Curr. Opin. Cell ... 5B, right panel). After cell division, the apical cell self-renews as a neuroblast stem cell, whereas the basal cell forms the ... Tepass, U., Tanentzapf, G., Ward, R. and Fehon, R. (2001). Epithelial cell polarity and cell junctions in Drosophila. Annu. Rev ...http://jcs.biologists.org/content/122/24/4570.long
A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity | JCB
... to specify basolateral vesicle delivery to nascent cell-cell contacts and immediately initiate cell surface polarity. ... but as cells make early contacts, Sec6 begins to localize to the initial cell-cell contacts (B) and accumulates at cell-cell ... Cell adhesion to other cells and the extracellular matrix is important in the generation of epithelial cell surface polarity. ... Novel function of the cell adhesion molecule uvomorulin as an inducer of cell surface polarity. Cell. 62:309-316. ...http://jcb.rupress.org/content/178/2/323
Apical targeting of syntaxin 3 is essential for epithelial cell polarity | JCB
Epithelial cell polarity depends on the accurate targeting of apical and basolateral plasma membrane proteins (Mostov et al., ... Instead, cells form tumor-like cell structures that lack tight junctions and exhibit no apparent cell polarity. Bars, 5 μm. (B ... Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly. Nat. Cell Biol. 3:831-838. ... For unknown reasons, cell polarity is more severely affected when MDCK cells are cultured in 3D collagen. In the case of ...http://jcb.rupress.org/content/173/6/937
Basolateral sorting and transcytosis define the Cu+-regulated translocation of ATP7B to the bile canaliculus | Journal of Cell...
2012). The clathrin adaptor AP-1A mediates basolateral polarity. Dev. Cell 22, 811-823. doi:10.1016/j.devcel.2012.02.004. ... ATP7B-loaded vesicles are rapidly relocated to the cell basolateral domain after their release from the TGN. Can 10 cells that ... Treatment of cells for 60 min with DYN before challenge with Cu+ showed a marked retention of ATP7B in the basolateral surface ... 2004). The subapical compartment: a traffic center in membrane polarity development. J. Cell Sci. 117, 2183-2192. doi:10.1242/ ...http://jcs.biologists.org/content/129/11/2190