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*  Patent US7005518 - Phthalocyanine dyes - Google Patents
Tabor, S. et al., "DNA sequence analysis with a modified bacteriophage T7 DNA polymerase" Proc. Natl. Acad. Sci. USA 84:4767-71 ... "DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-simplified DNA" Proc ... USA, 85: 9436 (1988)) or specially modified T7 DNA polymerase ("SEQUENASE") (see, S. Tabor and C. C. Richardson, Proc. Natl. ... The sequencing reactions are either performed in solution with the use of different DNA polymerases, such as the thermophilic ...
  http://www.google.co.uk/patents/US7005518
*  Alternative splicing of a precursor-mRNA encoded by the Chlorella sorokiniana NADP-specific glutamate dehydrogenase gene yields...
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Tabor, S; Richardson, CC ... Using miniprep plasmid DNA for sequencing double stranded templates with sequenase. Kraft, R; Tardiff, J; Krauter, KS; Leinwand ... Restriction enzyme analysis and cloning of high molecular weight genomic DNA isolated from Chlorella sorokiniana (Chlorophyta) ... Analysis of the genes encoding the largest subunit of RNA polymerase II in Arabidopsis and soybean ...
  https://www.deepdyve.com/lp/springer_journal/alternative-splicing-of-a-precursor-mrna-encoded-by-the-chlorella-EuZy0epWS9
*  A broad host range reporter plasmid for the analysis of divergent promoter regions
Tabor S. and Richardson C. (1987). DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl Acad. Sci ... These blunt-end DNA fragments were ligated and pMJ441, which encoded gus RU-AE01 promoter lacZ, RP4 oriT tet and the broad ... 6) Finally, pMJ441 was digested with Bgl II, to excise the RU-AE01 promoter fragment, and the DNA backbone re-circularized to ... We were able to introduce DNA fragments up to 1 kb between the genes for the reporter enzymes. We also successfully applied the ...
  http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0038-23532008000400013&lng=pt&nrm=iso&tlng=en
*  Tran NQ[au] - PubMed - NCBI
Thioredoxin, the processivity factor, sequesters an exposed cysteine in the thumb domain of bacteriophage T7 DNA polymerase. ... Hydrophobic Residue in Escherichia coli Thioredoxin Critical for the Processivity of T7 DNA Polymerase. ... Helicase-DNA polymerase interaction is critical to initiate leading-strand DNA synthesis. ... Characterization of a nucleotide kinase encoded by bacteriophage T7.. Tran NQ, Tabor S, Amarasiriwardena CJ, Kulczyk AW, ...
  https://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Tran+NQ%5Bau%5D&dispmax=50
*  Complete sequences of DQA1 and DQB1 cDNA clones corresponding to the DQw4 specificity | SpringerLink
Tabor, S. and Richardson, C. C.: DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.Proc Natl Acad Sci USA ... Gyllensten, U. B. and Erlich, H. A.: Generation of single-stranded DNA by the polymerase chain reaction and its application to ...
  https://link.springer.com/article/10.1007/BF02421214
*  Patent US20080032295 - Sensing apparatus and method - Google Patents
... polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension ... DNA Sequence Analysis with a Modified bacteriophage T7 DNA polymerase. Effect of pyrophosphorolysis and metal ions', J. Biol. ... Recombinant T7 polymerase may be used instead of taq polymerase. Where T7 polymerase is used, this may provide increased speed ... The DNA synthesis is followed by electrophoresis of the synthesised DNA to separate DNA molecules according to their mass to ...
  http://www.google.com/patents/US20080032295?dq=7245279
*  US Patent # 5,587,361. Oligonucleotides having phosphorothioate linkages of high chiral purity - Patents.com
... luteus polymerase, T4 bacteriophage polymerase, modified T4 DNA polymerase, T7 RNA polymerase and E. coli RNA polymerase. The ... Such polymerases include but are not necessarily limited to T7 DNA polymerase, modified T7 DNA polymerases such as the above ... employed T7 coliphane DNA having seventeen promoters and one termination site for T7 RNA polymerase. In vitro synthesis by T7 ... Brody, R. et al., "Stereochemical coruse of nucleotidyl catalyzed by bacteriophage T7 induced DNA polymerase", Biochemistry ...
  http://patents.com/us-5587361.html
*  Patent US7416844 - Composition for nucleic acid sequencing - Google Patents
The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at ... A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic ... See Huber et al., "Escherichia coli Thioredoxin Stabilizes Complexes of Bacteriophage T7 DNA Polymerase and Primed Templates," ... coli DNA polymerase, the Klenow fragment of E. coli DNA polymerase, T4 DNA polymerase, T7 DNA polymerase, and others. Examples ...
  http://www.google.com/patents/US7416844?dq=D523,461
*  Polbase - Reference: A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for...
A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between ... Bacteriophage T7 DNA polymerase efficiently incorporates a ... Bacteriophage T7 DNA polymerase efficiently incorporates a chain ... A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between ... coli DNA polymerase I (position 762) or T. aquaticus DNA polymerase (position 667) with tyrosine decreases discrimination ...
  https://polbase.neb.com/references/196
*  Patente US20060188900 - High speed nucleic acid sequencing - Google Patentes
The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at ... A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic ... See Huber et al., "Escherichia coli Thioredoxin Stabilizes Complexes of Bacteriophage T7 DNA Polymerase and Primed Templates," ... coli DNA polymerase, the Klenow fragment of E. coli DNA polymerase, T4 DNA polymerase, T7 DNA polymerase, and others. Examples ...
  http://www.google.es/patents/US20060188900?hl=es&dq=flatulence
*  T7 DNA polymerase - Wikipedia
T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase ... It is a member of the Family A DNA polymerases, which include E. coli DNA polymerase I and Taq DNA polymerase. This polymerase ... Nucleotidyl transfer by DNA polymerase. T7 DNA polymerase catalyzes the phosphoryl transfer during DNA replication of the T7 ... Instead, the T7 DNA polymerase complex requires only three proteins for processive DNA polymerization: T7 polymerase (gp5), ...
  https://en.wikipedia.org/wiki/T7_DNA_polymerase
*  High Energy Collisional Activation of Biomolecules - Robert Townsend
2017) Hybrid Methods Reveal Multiple Flexibly Linked DNA Polymerases within the Bacteriophage T7 Replisome. Structure 25:157- ... DNA Methylation and Inflammatory Signatures Associated with Suicide Risk and Treatment in US Veterans ...
  http://grantome.com/grant/NIH/P41-GM103422-41-5239
*  Introduction
Bacteriophage T7 RNA polymerase is a DNA-dependent RNA polymerase that is encoded in the T7 bacteriophage genome. The enzyme ... Genetic Analysis of T7 RNA Polymerase:. The genome of bacteriophage T7 was sequenced by Dunn et. al. 1983 and Moffatt et. al., ... The coding region of T7 RNA polymerase is located between nucleotides 3170 and 5819. Thus T7 RNA polymerase consists of 883 ... Figure 1. Schematic representation summarizing the most important features of bacteriophage T7 RNA polymerase. [Constructed ...
  http://academic.brooklyn.cuny.edu/chem/zhuang/Nicolas/Introduction.htm
*  Protein Enzyme Kinetics - Olis, Inc.
2005). Kinetics of Nucleotide Incorporation Opposite DNA Bulky Guanine N2 Adducts by Processive Bacteriophage T7 DNA Polymerase ... 2008). Kinetic Analysis of Correct Nucleotide Insertion by a Y-Family DNA Polymerase Reveals Conformational Changes Both Prior ... 2014). Elucidation of Kinetic Mechanisms of Human Translesion DNA Polymerase ? Using Tryptophan Mutants.. FEBS Journal 281, ... 2005). Kinetic Analysis of Steps in the Repair of Damaged DNA by Human 06-alkylguanine DNA Alkyltransferase.. J. Biological ...
  http://olisweb.com/applications/protein-enzyme-kinetics/
*  WO1997004126A1 - Method for amplifying nucleic acid sequences by translocation using chimeric primers - Google Patents
A cyclic amplification is achieved that may be implemented isothermally on the basis of either a DNA or an RNA target even when ... and a DNA- or RNA-type primer B1 capable of hybridising with said 3'-terminal segment of the target. ... terminal segment of the target and a DNA-type segment capable of hybridising with a segment adjacent to said 3'-terminal ... A target nucleic acid sequence (12) may be amplified in the presence of an enzymatic system including DNA polymerase, strand ...
  https://patents.google.com/patent/WO1997004126A1/en
*  Relaxed rotational and scrunching changes in P266L mutant of T7 RNA polymerase reduce short abortive RNAs while delaying...
bacteriophage T7 RNA polymerase. *DNA-Directed RNA Polymerases. Grant support. *R01 GM065367/GM/NIGMS NIH HHS/United States ... Concentration of T7 RNAP-DNA, GTP, and NTPs was 10 nM, 1 mM, and 500 µM, respectively. (C) The fraction of EC versus time was ... D) A complex of WT or P266L T7 RNAP (2 µM) and promoter DNA (1 µM) was mixed with limited NTPs or NTP and 3′-deoxy NTP mixture ... Both WT and P266L T7 RNAP bind, bend, and open the promoter DNA from −4 to +2 to the same extent. The rigid C-linker of WT ...
  https://www.ncbi.nlm.nih.gov/pubmed/24651161
*  Group I catalytic intron - Wikipedia
Bonocora RP, Shub DA (December 2004). "A self-splicing group I intron in DNA polymerase genes of T7-like bacteriophages". J. ... T-even and T7-like bacteriophages. Both intron-early and intron-late theories have found evidences in explaining the origin of ... Two-metal-ion mechanism seen in protein polymerases and phosphatases was proposed to be used by group I and group II introns to ... Lee CN, Lin JW, Weng SF, Tseng YH (December 2009). "Genomic characterization of the intron-containing T7-like phage phiL7 of ...
  https://en.wikipedia.org/wiki/Group_I_catalytic_intron
*  Processivity - Wikipedia
"The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I". ... Tabor, S; Richardson, C. C. (1987). "DNA sequence analysis with a modified bacteriophage T7 DNA polymerase". Proceedings of the ... When associated with a DNA clamp, DNA polymerase is dramatically more processive; without the clamp most polymerases have a ... "DNA polymerase switching: I. Replication factor C displaces DNA polymerase α prior to PCNA loading". Journal of Molecular ...
  https://en.wikipedia.org/wiki/Processivity
*  EP0745686A1 - The use of DNA polymerase 3'-intrinsic editing activity - Google Patents
The use is concerned with the incorporation of dNTPs into DNA templates in order to determine the concentration and / or ... The invention is directed to the use of RNA or DNA polymerases having 3'-intrinsic editing activity (3'-IEA) in the presence or ... Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA ... coli DNA polymerase I large fragment and T7 DNA polymerase. This was not the case for Taq DNA polymerase nor several other ...
  https://patents.google.com/patent/EP0745686A1/en
*  Protein moonlighting - Wikipedia
Upon infection with the bacteriophage T7, E. coli thioredoxin forms a complex with T7 DNA polymerase, which results in enhanced ... T7 DNA replication, a crucial step for successful T7 infection. Thioredoxin binds to a loop in T7 DNA polymerase to bind more ... The anti-oxidant function of thioredoxin is fully autonomous and fully independent of T7 DNA replication, in which the protein ... DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the ...
  https://en.wikipedia.org/wiki/Protein_moonlighting
*  Myricetin - Wikipedia
In T7 bacteriophages myricetin competitively inhibited DNA template binding to RNA polymerase. Myricetin has been seen to ... cellular DNA polymerase, and cellular RNA polymerase. Inhibition of cellular DNA polymerases could have dangerous effects on ... Inhibition of cellular RNA polymerase could have deleterious effects on the cell's capacity to transcribe and translate DNA and ... The resulting hydroxy radicals are often linked to DNA degradation, however, there are doubts as to whether or not this damage ...
  https://en.wikipedia.org/wiki/Myricetin
*  US Patent # 5,854,033. Rolling circle replication reporter systems - Patents.com
Primary structure of bacteriophage M2 DNA polymerase: conserved segments within protein-priming DNA polymerases and DNA ... Preferred gap-filling DNA polymerases are T7 DNA polymerase (Studier et al., Methods Enzymol. 185:60-89 (1990)), DEEP VENT.RTM ... Such DNA polymerases are referred to herein as gap-filling DNA polymerases. Unless otherwise indicated, a DNA polymerase ... Preferred rolling circle DNA polymerases are bacteriophage .phi.29 DNA polymerase (U.S. Pat. Nos. 5,198,543 and 5,001,050 to ...
  http://patents.com/us-5854033.html
*  High‐efficiency bypass of DNA damage by human DNA polymerase Q | The EMBO Journal
The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I. ... Cannistraro VJ, Taylor JS (2004) DNA-thumb interactions and processivity of T7 DNA polymerase in comparison to yeast polymerase ... Doublie S, Tabor S, Long AM, Richardson CC, Ellenberger T (1998) Crystal structure of a bacteriophage T7 DNA replication ... Zhang Y, Yuan F, Wu X, Taylor JS, Wang Z (2001) Response of human DNA polymerase iota to DNA lesions. Nucleic Acids Res 29: 928 ...
  http://emboj.embopress.org/content/23/22/4484
*  DNA-Directed DNA Polymerase - Polymerases, DNA Summary Report | CureHunter
DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze ... the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also ... possess exonuclease activity and therefore function in DNA repair. EC 2.7.7.7. ... bacteriophage RB69 DNA polymerase. *bacteriophage T7 induced DNA polymerase. *C elegans Polg-1 protein ...
  http://www.curehunter.com/public/keywordSummaryD004259.do
*  The T7 Group | SpringerLink
In this chapter I shall not try to review in detail the genetics and molecular biology of phage T7, since this topic has been ... Bacteriophage T7 deoxyribonucleic acid replication in vitro. Bacteriophage T7 DNA polymerase: An enzyme composed of phage-and ... coli DNA polymerase I showing sequence homology to T7 DNA polymerase, Nature 313: 818.Google Scholar ... Dunn, J. J., and Studier, F. W., 1983, Complete nucleotide sequence of bacteriophage T7 DNA and the location of T7 genetic ...
  https://link.springer.com/chapter/10.1007/978-1-4684-5424-6_8