Loading...


*  Many P-Element Insertions Affect Wing Shape in Drosophila melanogaster | Genetics
... includes a bacterial plasmid sequence with replication origin and ampicillin resistance to the right of an EcoRI restriction ... Plasmid rescue and sequencing:. A fragment of flanking genomic DNA to the right of each P{lacW} insertion site was retrieved by ... Clones surviving ampicillin were screened by electrophoresis. The flanking genomic DNA in typical clones was sequenced using ... Flanking DNA on the right-hand (3′) side of each insertion was retrieved by plasmid rescue, for all 12 insertions that passed ...
  http://www.genetics.org/content/169/3/1461
*  Plus it
... a linear bacterial plasmid vector containing an ampicillin resistance gene and other sequences unrelated to mouse genome, was ... A (forward) primer on the ampicillin gene 5′-ATAATACCGCGCCACATAGC-3′ and a (reverse) primer on the human β actin promoter 5′- ... and 10 μg/ml ampicillin. Coverslips with adherent cochlear cultures were washed once with HEPES-buffered (10 mm, pH 7.2) HBSS ( ... and 10 μg/ml ampicillin. The tissues were fixed in freshly prepared 4% (w/v) paraformaldehyde for 1 h and then washed three ...
  http://www.jneurosci.org/content/33/10/4395
*  PBLU - Wikipedia
pBLU is a commercially produced bacterial plasmid that contains genes for ampicillin resistance (beta lactamase and beta ... galactosidase). It is often used in conjunction with an ampicillin-susceptible E. coli strain to teach students about ... "BIOTECHNOLOGY: BACTERIAL TRANSFORMATION" (PDF). CollegeBoard. Retrieved 29 October 2017. ...
  https://en.wikipedia.org/wiki/PBLU
*  715. Chymosin produced from Aspergillus niger var. awamori/calf prochymosin B gene (WHO Food Additives Series 28)
The plasmid carries the ampicillin resistance gene as a selective marker for bacterial transformants carrying the prochymosin ... and the ampicillin-resistance gene carried by the plasmid was used as a marker for the selection of the transformed cells. ... The ampicillin resistance marker normally present in the pUC18 vector was not expressed in the production strain. The chymosin ... The pBR322 sequences retained in the expression vector contain the gene coding for ampicillin resistance. However, this gene is ...
  http://www.inchem.org/documents/jecfa/jecmono/v28je08.htm
*  Microbiology Society Journals | Characterization of Genes Specifying Ampicillin Resistance in Bacterial Isolates Using a Single...
... of Genes Specifying Ampicillin Resistance in Bacterial Isolates Using a Single-strand Specific Nuclease for Analysis of Plasmid ... of Genes Specifying Ampicillin Resistance in Bacterial Isolates Using a Single-strand Specific Nuclease for Analysis of Plasmid ...
  http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-111-1-233
*  Bacterial antibiotic resistance - Stock Video Clip K005/0360 - Science Photo Library
A loop of bacterial DNA called a plasmid (circle), carries genes for resistance to the antibiotics tetracycline and ampicillin ... The plasmid with disrupted resistance to tetracycline (but functional ampicillin resistance, represented as green) is ... Addition of ampicillin antibiotic kills the bacterial colonies which do not carry the plasmid. Subsequent treatment with ... tetracycline kills only the colonies which contain the plasmid with disrupted tetracycline resistance. - Stock Video Clip K005/ ...
  http://www.sciencephoto.com/media/681536/view
*  pcDNA4/His-Max B - Addgene Vector Database (Plasmids, Expression Vectors, etc)
Bacterial Resistance:. Ampicillin. Selectable Marker:. Zeocin. Catalog Number:. V86420. Stable:. Transient. Constitutive:. ... Addgene is a nonprofit plasmid repository. We archive and distribute high quality plasmids from your colleagues. ... You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more ...
  http://www.addgene.org/vector-database/2121/
*  pcDNA4/His C - Addgene Vector Database (Plasmids, Expression Vectors, etc)
Bacterial Resistance:. Ampicillin. Selectable Marker:. Zeocin. Notes:. Easy to clone into other vectors. ... Addgene is a nonprofit plasmid repository. We archive and distribute high quality plasmids from your colleagues. ... You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more ...
  http://www.addgene.org/vector-database/2119/
*  Molecular I pre-lab - Molecular I Pre-lab 1 What is a plasmid How is it different from genomic or chromosomal DNA a A plasmid...
Molecular I Pre-lab 1. What is a plasmid? How is it different from genomic or chromosomal DNA? a. A plasmid is an extra ... a. The plasmid DNA can confer a different phenotype to the bacterial cell. The plasmid pAMP confers resistance to ampicillin to ... A plasmid by itself cannot replicate itself. So, a plasmid does not grow. In the presence of UV light, the plasmid we will use ... A cell that obtains this plasmid can survive in the presence of the antibiotic ampicillin, but cells that do not contain this ...
  https://www.coursehero.com/file/5794674/Molecular-I-pre-lab/
*  Pediatric Bacterial Meningitis: Practice Essentials, Background, Pathophysiology
Pediatric bacterial meningitis is a life-threatening illness that results from bacterial infection of the meninges and leaves ... Plasmid-mediated resistance to ampicillin due to the production of beta-lactamase enzymes by bacterium is increasingly being ... Pediatric bacterial meningitis is a life-threatening illness that results from bacterial infection of the meninges and leaves ... Pediatric bacterial meningitis is a life-threatening illness that results from bacterial infection of the meninges. Because ...
  https://emedicine.medscape.com/article/961497-overview
*  View source - 2011.igem.org
GFP plasmid that confers ampicillin resistance. Activation ofCRISPER-gfp destroys the gfp-containing plasmid restoring the ... Team:Colombia , Team Colombia]]: Bacterial prevention of rust headaches==== Our objective is to create a bacterial "detect and ... We exploited this system by engineering E.coli with an inducible mechanism of self-curing plasmid based antibiotic resistance. ... Team:USC , Team USC]]: Bioengineering a mechanism to override plasmid-based antibiotic resistance==== Bacteria protect their ...
  http://2011.igem.org/wiki/index.php?title=Jamboree/Team_Abstracts&action=edit
*  Biotechnology: Bacterial Transformation Lab by Ezra Hall on Prezi
In our lab we used E.Coli bacteria that is going to be transformed to incorporate a pGLO plasmid with ampicillin resistance, an ... Ampicillin is an antibiotic that would usually kill the cell but this is prevented by the gene on the pGLO with resistance to ... This leads us to believe that the pGLO plasmid did not combine with the E.coli DNA. Our plate with ampicillin also did not have ... Plasmids are usually used in bacterial transformation because they are small circular DNA molecules that are easily ...
  https://prezi.com/xoabaazt0nqt/biotechnology-bacterial-transformation-lab/
*  pMOD2-Zeo : CpG-free Sh ble Zeocin resistance modified gene - InvivoGen
Resistance to the antibiotic Zeocin� is conferred by the Sh ble gene from Streptoalloteichus hindustanus. The DNA sequence of ... The CpG-free Sh ble gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin. To facilitate the ... Resistance to the antibiotic Zeocin™ is conferred by the Sh ble gene from Streptoalloteichus hindustanus. The DNA sequence of ... 20 µg of lyophilized pMOD2-Zeo plasmid DNA. • 4 pouches of E. coli Fast-Media® Amp (2 LB and 2 Agar) ...
  https://www.invivogen.com/pmod2-zeo
*  RNAi in C. elegans | Protocol
The plasmid contains a gene for resistance to the antibiotic ampicillin, which can be used to select for bacterial colonies ... and add it to 1 ml of LB broth containing 100 μg/ml of ampicillin to select for bacteria containing the plasmid. Incubate ... In the case of C. elegans, the worms are fed bacteria, which have been transformed with plasmids that code for double stranded ... After the overnight incubation, add 5 ml of LB broth containing 100 μg/ml of ampicillin to the culture, and incubate for an ...
  https://www.jove.com/science-education/5105/rnai-in-c-elegans
*  A Versatile and Reliable Two-Component System for Tissue-Specific Gene Induction in Arabidopsis | Plant Physiology
... which can be used for plasmid rescue procedures because it encodes an ampicillin-resistance gene for bacterial selection and ... bacterial chloramphenicol resistance; ccdB, bacterial toxin gene for negative selection. ... Plasmid Construction. Standard gene-cloning methods (Sambrook and Russell, 2001) were used to make the constructs. An attR1-Cmr ... The DT-A entry clone was generated by amplifying the DT-A chain from plasmid pIBI30-DT-A (Maxwell et al., 1986; Harrison et al ...
  http://www.plantphysiol.org/content/141/4/1194.full
*  Addgene: pET21a.MJ1594
Plasmid pET21a.MJ1594 from Dr. Sung-Hou Kim's lab contains the insert phosphoserine phosphatase and is published in Structure. ... Please note that the plasmid pET21a.MJ1594 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a ... Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244 ... Plasmid. 11413. Plasmid sent as bacteria in agar stab 1. $65 Add to Cart ...
  https://www.addgene.org/11413/
*  Transformation of E. coli with pGAL™ (Blue Colony)™
... acquire antibiotic resistance and a blue color. The plasmid used is unique and designed to simplify the process. ... following uptake of plasmid carried beta-galactosidase and ampicillin resistance genes. The plasmid used, pGAL™, is unique, and ... In this experiment, the bacterial "transformants" acquire antibiotic resistance and a blue color, ... acquire antibiotic resistance and a blue color. The plasmid used is unique and designed to simplify the process. ...
  https://www.flinnsci.com/transformation-of-e.-coli-jm101-with-pgal/fb0313/
*  Ampicillin resistance - Wikipedia
This is done by inserting the insulin gene into a vector, or plasmid, and allowing bacterial cells to transformation. ... Ampicillin resistance is bacterial resistance to the antibiotic ampicillin. Many strains of bacteria are resistant to a variety ... however the resistance that is most commonly used in research and industry is ampicillin resistance. It is used as a selectable ... Although ampicillin resistance can be a huge benefit to scientists, it can also cause problems in regards to medical treatment ...
  https://en.wikipedia.org/wiki/Ampicillin_resistance
*  Need Help: Bacterial gene deletion- sacB sucrose resistance - Molecular Biology - BioForum
... sacB sucrose resistance - posted in Molecular Biology: Hi All, I have a problem regarding selecting allelic exchange mutants. I ... dependent origin of replication from plasmid R6K and gene encoding resistance to ampicillin and kanamycin but no sacB gene ... That will take away the pressure to maintain the kan-amp resistance provided by the integrated plasmid. That plasmid then could ... That will take away the pressure to maintain the kan-amp resistance provided by the integrated plasmid. That plasmid then could ...
  http://www.protocol-online.org/forums/topic/9924-need-help-bacterial-gene-deletion-sacb-sucrose-resistance/
*  Bacterial Transformation: PASCO
Selecting for transformed cells by selecting for ampicillin resistance.. Materials Used:. Calcium chloride solution - 500 ul. ... pAmp plasmid - 10 ul. Sterile test tubes, 15-mL - 2. Sterile, glass spreading rod Water bath (42ºC) - 1 per class. Wax labeling ... Bacterial Transformation. Lab Summary. Use the tools of biotechnology to transform competent E.coli cells and then select for ... Home / Products / Lab Manuals / College Biology Instructor Guide / Bacterial Transformation. Featured in PASCO's College ...
  https://www.pasco.com/products/experiments/college-biology/bacterial-transformation.cfm
*  RK2 plasmid - Wikipedia
... as one of a family of plasmids implicated in transfer of Ampicillin resistance between bacterial strains. Plasmids in the IncP- ... The resistance genes confer resistance to the antibiotics kanamycin, ampicillin and tetracycline. In addition, RK2 contains a ... which confer resistance to Ampicillin and Chloramphenicol, respectively. Minimal plasmids such as PFF1 are useful for studying ... "Bacterial partitioning proteins affect the subcellular location of broad-host-range plasmid RK2", Plasmid, 2010 Nov;64(3):119- ...
  https://en.wikipedia.org/wiki/RK2_plasmid
*  Document
... ampicillin resistance) pGLO plasmids Bacterial DNA Bacterial cell Plasmid DNA Genomic DNA Transcriptional Regulation • Lactose ... often a circular plasmid GFP Beta-lactamase Ampicillin Resistance What is a plasmid? • A circular piece of autonomously ... ampicillin resistance) What is Nutrient Broth? • Luria-Bertani (LB) broth • Medium that contains nutrients for bacterial growth ... Ampicillin resistance • Green Fluorescent Protein (GFP) - Aequorea victoria jellyfish gene • araC regulator protein - Regulates ...
  http://slidegur.com/doc/3390389/document
*  Structure, Isolation, and Function of DNA (B1) | Modern Biology Inc. Creative Experiments used in teaching labs of College...
... coli by introducing into bacterial cells a plasmid that contains an ampicillin-resistance gene. Sufficient sterile materials ... The introduction of plasmid DNA into bacterial cells is called transformation and has enabled scientists to obtain large ... Occasionally, E. coli cells are found in nature that are resistant to the toxic effects of the antibiotic ampicillin. In this ... exercise, students create such an ampicillin-resistant population of E. ...
  http://modernbio.com/structure-isolation-and-function-dna
*  Patent US6391637 - Use of CD40 ligand, a cytokine that binds CD40, to stimulate hybridoma cells - Google Patents
... pBR322 contains genes for ampicillin and tetracycline resistance and thus provides simple means for identifying transformed ... vectors for prokaryotic host cells include a selectable marker of bacterial origin derived from commercially available plasmids ... The construct was prepared by cutting the appropriate DNA from the a plasmid containing human CD40-L (derived from the plasmid ... Plasmid DNA was isolated from pools consisting of approximately 2,000 clones of transformed E. coli per pool. The isolated DNA ...
  http://www.google.com.au/patents/US6391637
*  US Patent # 9,770,011. Veterinary composition and methods for non-surgical neutering and castration - Patents.com
... such as ampicillin resistance. Other components of the plasmid may include an origin of replication and an amplicon, such as ... For example, bacterial leader sequences may be selected (e.g., for in vitro expression of the antibody or other applications). ... The expression cassette can be carried on any suitable vector, e.g., a plasmid, which is delivered to a host cell. The plasmids ... The plasmids may also contain certain selectable reporters or marker genes that can be used to signal the presence of the ...
  http://patents.com/us-9770011.html