*  Isolation of cell-free bacterial inclusion bodies | Microbial Cell Factories | Full Text
Although many IB purification protocols have been developed, their effectiveness regarding residual cell viability had not been ... Viable BacteriumViable Cell CountBacterial LysisLysozyme TreatmentSonication Cycle. Background. Bacterial inclusion bodies (IBs ... all of them including bacterial lysis and IB washing steps. Bacterial lysis is achieved using either mechanical or non ... we have determined the number of viable bacterial cells before and after cell lysis, using different bacterial strains (MC4100 ...
  https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-9-71
*  Experimentally Induced Anachoresis in the Periapical Region After Root Canal Filling
... since they might also reduce the viability of some microbial cells (Block, 1991). The presence of other microbial groups in ... Microbial adhesion to the periapical tissues may have impaired isolation of bacteria, even after maceration of the periapical ... The bacterial cells were washed three times in 1.5 ml of ultra-pure Milli Q water by centrifuging at 8,000 x g for 5 min. and ... This highlights the importance of methodologies that do not depend on microbial viability, such as PCR (Polymerase Chain ...
  http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2012000100001&lng=en&nrm=iso&tlng=en
*  Use of lysozyme, nisin, and EDTA combined treatments for maintaining quality of packed ostrich patties.
Lactobacillaceae / drug effects, isolation & purification. Meat Products* / analysis, microbiology. Microbial Viability / drug ... Anti-Bacterial Agents / pharmacology*. Antioxidants / pharmacology. Dose-Response Relationship, Drug. Edetic Acid / ... 0/Anti-Bacterial Agents; 0/Antioxidants; 0/Thiobarbituric Acid Reactive Substances; 1414-45-5/Nisin; 9G34HU7RV0/Edetic Acid; EC ... Enterobacteriaceae / drug effects, isolation & purification. Food Microbiology*. Food Packaging / methods. Food Preservation / ...
  http://www.biomedsearch.com/nih/Use-lysozyme-nisin-EDTA-combined/20492308.html
*  Growth medium - Wikipedia
... and organic growth factors so as to prevent microbial multiplication Transport media used in the isolation of anaerobes must be ... Certain bacterial inhibitors are used for gonococci, and buffered glycerol saline for enteric bacilli. Venkataraman Ramakrishna ... Temporary storage of specimens being transported to the laboratory for cultivation Maintain the viability of all organisms in ... YM (yeast extract, malt extract agar) has a low pH, deterring bacterial growth. MacConkey agar is for Gram-negative bacteria. ...
  https://en.wikipedia.org/wiki/Growth_medium
*  Bacterial strain, processed plant extracts, compositions containing same, processes for their preparation and their...
The microbial biomass could be kept in saline in the refrigerator for 48 hours without loosing viability.. Example 3. ... Isolation and Growth of the Probiotic Organism E. coli ATCC Deposit No. 202226. The probiotic organism E. coli (deposited at ... This latter phenomenon has been describedunder such names as `bacterial antagonism`, `bacterial interference`, `barrier effect ... The bacterial strains identified byany such test are within the category of probiotic bacteria, as the term is used herein.. ...
  http://www.patentgenius.com/patent/6500424.html
*  Enhancement of biofilm-mode growth of CoNS cells by oxa | Open-i
Microbial Viability/drug effects. *Microscopy, Confocal. *Microscopy, Fluorescence/methods. *Polysaccharides, Bacterial/ ... Bottom Line: Epifluorescence microscopy and confocal laser scanning microscopy in combination with bacterial viability staining ... Bottom Line: Epifluorescence microscopy and confocal laser scanning microscopy in combination with bacterial viability staining ... Epifluorescence microscopy and confocal laser scanning microscopy in combination with bacterial viability staining and ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2902406_1476-0711-9-16-1&req=4
*  Enumeration and quantification of M. leprae in mouse FP | Open-i
... viability was markedly decreased by 8 weeks in immunocompetent mice.Rifapentine significantly reduced bacterial viability after ... leprae in tissues without the need for bacterial isolation or immediate processing, making these assays applicable for in vivo ... viability was markedly decreased by 8 weeks in immunocompetent mice.Rifapentine significantly reduced bacterial viability after ... In contrast, viability was markedly decreased by 8 weeks in immunocompetent mice. Rifapentine significantly reduced bacterial ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC3750008_pntd.0002404.g001&req=4
*  Resetting microbiota by Lactobacillus reuteri inhibits T reg deficiency-induced autoimmunity via adenosine A2A receptors | JEM
... our results also showed reduced gut microbial diversity and altered bacterial composition in T reg cell-deficient SF mice, ... Naive CD4+ T cell isolation and in vitro differentiation to Th1 and Th2. Naive CD4+ T cells were isolated from the spleens of 6 ... After 96 h, cell viability was measured by TACS XTT cell proliferation assay kit (Trevigen, Inc.). ... Gut microbial Shannon diversity analysis (two-way ANOVA; n = 6-12 per group). (D) Gut microbial analysis of weighted unifrac- ...
  http://jem.rupress.org/content/early/2016/12/22/jem.20160961
*  Lipid Metabolism sub-cluster 73
Column fractionation and purification of an n-hexane fraction led to the isolation of three lupeol-based triterpenoid esters ... New chemical-enzymatic technology based on the modification of the bacterial polysaccharide K5 from Escherichia coli leads to ... Prebiotic Fructooligosaccharides (FOS) escape metabolism in upper GI tract undergo microbial metabolism in colon and thereby ... In vitro fermentation of prebiotics by Lactobacillus plantarum CFR 2194: selectivity, viability ... ...
  http://www.biomedsearch.com/cluster/14/Lipid-Metabolism/sub-73-p10.html
*  A microbial world within us - Zoetendal - 2006 - Molecular Microbiology - Wiley Online Library
The host genotype affects the bacterial community in the human gastrointestinal tract. Microbial Ecol Health Dis 13: 129-134. * ... Isolation and characterization of GI tract microbes have given us insight into their physiology and potential roles in the GI ... Viability and metabolic activity. While a first step in studying ecosystems is the identification of their members, a ... Construction and analysis of bacterial artificial chromosome libraries from marine microbial assemblage. Environ Microbiol 2: ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05056.x/full
*  ASMscience | Community Structure: Bac
The PCR methods have been used with samples from sediments and microbial mats, as well as deep-sea holothurian guts, often ... showing unexpected bacterial and archaeal diversity. Some studies have used information about the 16S rRNA clones short of ... The oldest method for obtaining information on microbial community structure is to examine the sample with a microscope and ... Button, D. K.,, F. Schuts,, P. Quang,, R. Martin, and, B. R. Robertson. 1993. Viability and isolation of marine bacteria by ...
  http://www.asmscience.org/content/book/10.1128/9781555815882.ch35
*  Metagenomic Analysis of Stress Genes in Microbial Mat Communities from Antarctica and the High Arctic
Analysis of bacterial exopolysaccharides. Biotechnol. Appl. Biochem. 9:12-19 [PubMed]. 23. Klein W, Weber MHW, Marahiel MA. ... Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol. 36:251-258 ... Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures. ... Recent developments in bacterial cold-shock response. Curr. Issues Mol. Biol. 6:125-136 [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3255749/?lang=en-ca
*  Burkholderia pseudomallei - Wikipedia
... viability, and isolation from contaminated specimens". J Bacteriol. 55 (1): 115-126. PMC 518415 . PMID 16561426. Wuthiekanun V ... March 2007). "Suitability of partial 16S ribosomal RNA gene sequence analysis for the identification of dangerous bacterial ... Microbial Pathogenesis. 36 (5): 287-9. doi:10.1016/j.micpath.2004.01.001. PMID 15043863. Nandi T, Ong C, Singh AP, Boddey J, ... Rose, L. J.; O'Connell, H. (2009-05-01). "UV Light Inactivation of Bacterial Biothreat Agents". Applied and Environmental ...
  https://en.wikipedia.org/wiki/Burkholderia_pseudomallei
*  Genetics - Medical Microbiology - NCBI Bookshelf
These latter characteristics are often selected as the inherited traits to be analyzed in studies of bacterial genetics. ... Bacterial viruses (bacteriophages or phages) have DNA or RNA as genetic material. The two essential functions of genetic ... Plasmids usually encode traits that are not essential for bacterial viability, and replicate independently of the chromosome. ... Isolation of lactose-negative (phenotype Lac-) mutants of E. coli poses a different problem. On minimal media with lactose as ...
  https://www.ncbi.nlm.nih.gov/books/NBK7908/?report=reader
*  Robotic Instruments Products, Reviews and Suppliers on SelectScience
QUANTOM Tx™ Microbial Cell Counter. Logos Biosystems, Inc.. Single bacterial cell detection - Rapid quantification - No ... Cellometer Auto 2000 Cell Viability Counter. Nexcelom Bioscience. All-in-one, touch-screen instrument for viability of primary ... DNA / RNA Isolation Purification * Evidence Collection & Analysis * Light Microscopy * Toxicology / Drugs of Abuse Testing ... The LUNA-FL™ can even detect and accurately measure the count and viability of primary cells such as PBMCs, splenocytes, ...
  http://www.selectscience.net/robotic-instruments/product-directory/?u=AC3B4D5D-08BB-44AD-98FD-5E42BCB6F7A6
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Immunology, Issue 86, Gram-Positive Bacterial Infections, Pneumonia, Bacterial, Lung Diseases, Respiratory Tract Infections, ... Biofilm cell viability is assayed after the recovery period. The MBC-P for the antimicrobial agent is defined as the lowest ... The microbial community encased within the biofilm often shows the differentiation of distinct subpopulation of specialized ... In nature, bacteria rarely exist in isolation; they are instead surrounded by a diverse array of other microorganisms that ...
  https://www.jove.com/visualize/abstract/21187931/prophage-spontaneous-activation-promotes-dna-release-enhancing
*  AGRIORISSA: November 2012
Broth and Agar Bacterial Growth Media. The two main types of bacterial growth media used are liquid broth and solid, Jell-o- ... transport media used in the isolation of anaerobes must be free of molecular oxygen.. Examples of transport media include:. ... maintain the viability of all organisms in the specimen without altering their concentration.. contain only buffers and salt.. ... Yeasts have recently been used to generate electricity in microbial fuel cells, and produce ethanol for the biofuel industry.. ...
  http://agriorissa.blogspot.com/2012/11/
*  Soluble Trem-like Transcript-1 Regulates Leukocyte Activation and Controls Microbial Sepsis | The Journal of Immunology
The effect of LR17 was also studied on bacterial clearance. As expected, very high bacterial counts were observed in the ... The viability of mice was examined every hour, and animals were sacrificed at regular intervals for blood sampling. ... Isolation of human polymorphonuclear cells and monocytes. Human peripheral blood samples were collected on EDTA from healthy ... G) Bacterial counts in spleen and blood obtained 24 h after CLP. (H and I) Survival curves (n = 25-40 per group) after CLP ...
  http://www.jimmunol.org/content/188/11/5585.long
*  Functional characterization of two defensins, HlDFS1 and HlDFS2, from the hard tick Haemaphysalis longicornis | Parasites &...
The cell viability assay was determined by Cell Viability Assay (Promega, Fitchburg, USA). ... Isolation of defensin genes HlDFS1 and HlDFS2 from H. longicornis By sequencing a cDNA library of H. longicornis, two cDNA ... Louis, USA) [21]. Approximately 90 μl of inocula of microbial strains (diluted with PB media to an OD600 nm of 0.001) were ... Antimicrobial activity was evaluated by measuring the absorbance of the bacterial culture at 595 nm. Borrelia burgdorferi 297- ...
  https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-017-2397-9
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and a comprehensive description of the viral and bacterial communities (via direct viral and microbial counts, enumeration of ... The third viability assay is a commonly used luminescent assay for ATP, but we use a quarter of the recommended volume to save ... For this, we accompany primary cell isolations of mouse VMs and/or AMs with critical quality control steps and direct membrane ... Viability Assays for Cells in Culture. Authors: Jessica M. Posimo, Ajay S. Unnithan, Amanda M. Gleixner, Hailey J. Choi, Yiran ...
  https://www.jove.com/visualize/abstract/24937478/community-shifts-surface-microbiomes-coral-porites-astreoides-with
*  Growth and survival of bacteria implicated in sudden infant death syndrome on cot mattress materials.
Microbial Viability*. Polyurethanes. Staphylococcus aureus / growth & development, isolation & purification. Streptococcus ... aureus on PU at low RH could explain, in the context of the common bacterial toxins hypothesis, an increased risk of SIDS ... Colony Count, Microbial. Colorimetry / methods. Culture Media. Escherichia coli / growth & development, isolation & ... 24013829 - The microbial spectrum of neonatal sepsis in uganda: recovery of culturable bacteria in.... 22418619 - Body ...
  http://www.biomedsearch.com/nih/Growth-survival-bacteria-implicated-in/16108799.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... of transgenic mice and defined bacterial mutants makes this model particularly suitable for identifying both host and microbial ... Biofilm cell viability is assayed after the recovery period. The MBC-P for the antimicrobial agent is defined as the lowest ... In nature, bacteria rarely exist in isolation; they are instead surrounded by a diverse array of other microorganisms that ... Gram-Positive Bacterial Infections, Bacteriology, Staphylococcus aureus, iron acquisition, hemoglobin, bacterial growth, ...
  https://www.jove.com/visualize/abstract/25375181/the-porphyromonas-gingivalis-ferric-uptake-regulator-orthologue-binds
*  Impacts of Climate Change on Indirect Human Exposure to Pathogens and Chemicals from Agriculture
... and flooding will increase mobility of microbial pathogens around the landscape. Therefore, environmental levels of microbial ... Harrus S, Baneth G. Drivers for emergence and re-emergence of vector-borne protozoal and bacterial diseases. Int J Parasitol. ... The impact of climate change on the behavior, viability, and fate of pathogens in the environment, and the stability and ... The various pathways and processes should not be considered in isolation because different climate-sensitive factors may have ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2679592/?lang=en-ca
*  Impact of ypfP deletion on cell wall proteins. A. Surfa | Open-i
Genes, Bacterial*. *Lipopolysaccharides/chemistry/isolation & purification/metabolism*. *Mutation/genetics*. *N-Acetylmuramoyl- ... Microbial Viability/drug effects. *Sodium Chloride/pharmacology. *Surface Properties. Related in: MedlinePlus ... C. Spontaneous bacterial autolysis. Data represent the means ± SD of three independent experiments. Mentions: Lipoteichoic acid ... C. Spontaneous bacterial autolysis. Data represent the means ± SD of three independent experiments. ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2169524_mmi0065-1078-f7&req=4
*  GLC-MS spectra of the per-O-acetylated glycan isolated | Open-i
Genes, Bacterial*. *Lipopolysaccharides/chemistry/isolation & purification/metabolism*. *Mutation/genetics*. *N-Acetylmuramoyl- ... Microbial Viability/drug effects. *Sodium Chloride/pharmacology. *Surface Properties. Related in: MedlinePlus ... A Staphylococcus aureus ypfP mutant with strongly reduced lipoteichoic acid (LTA) content: LTA governs bacterial surface ... A Staphylococcus aureus ypfP mutant with strongly reduced lipoteichoic acid (LTA) content: LTA governs bacterial surface ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2169524_mmi0065-1078-f3&req=4