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*  Isolation of cell-free bacterial inclusion bodies | Microbial Cell Factories | Full Text
Although many IB purification protocols have been developed, their effectiveness regarding residual cell viability had not been ... Viable BacteriumViable Cell CountBacterial LysisLysozyme TreatmentSonication Cycle. Background. Bacterial inclusion bodies (IBs ... all of them including bacterial lysis and IB washing steps. Bacterial lysis is achieved using either mechanical or non ... we have determined the number of viable bacterial cells before and after cell lysis, using different bacterial strains (MC4100 ...
  https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-9-71
*  Experimentally Induced Anachoresis in the Periapical Region After Root Canal Filling
... since they might also reduce the viability of some microbial cells (Block, 1991). The presence of other microbial groups in ... Microbial adhesion to the periapical tissues may have impaired isolation of bacteria, even after maceration of the periapical ... The bacterial cells were washed three times in 1.5 ml of ultra-pure Milli Q water by centrifuging at 8,000 x g for 5 min. and ... This highlights the importance of methodologies that do not depend on microbial viability, such as PCR (Polymerase Chain ...
  http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2012000100001&lng=en&nrm=iso&tlng=en
*  Final Report Summary - CFMATTERS (Cystic Fibrosis Microbiome-determined Antibiotic Therapy Trial in Exacerbations: Results...
CFMATTERS offers a stratified next generation approach to antibacterial treatment in a disease model of chronic bacterial ... The essential functions of a bacterial pathogen reflect the most basic processes required for its viability and growth, and ... For example, our work indicates that regional isolation drives bacterial diversification within CF lungs. While it was is known ... 2.1.6 WP 05 Microbial activities and functions. WP05 will use metatranscriptomics and metabolomics to assess bacterial gene ...
  https://cordis.europa.eu/result/rcn/227029_en.html
*  Use of lysozyme, nisin, and EDTA combined treatments for maintaining quality of packed ostrich patties.
Lactobacillaceae / drug effects, isolation & purification. Meat Products* / analysis, microbiology. Microbial Viability / drug ... Anti-Bacterial Agents / pharmacology*. Antioxidants / pharmacology. Dose-Response Relationship, Drug. Edetic Acid / ... 0/Anti-Bacterial Agents; 0/Antioxidants; 0/Thiobarbituric Acid Reactive Substances; 1414-45-5/Nisin; 9G34HU7RV0/Edetic Acid; EC ... Enterobacteriaceae / drug effects, isolation & purification. Food Microbiology*. Food Packaging / methods. Food Preservation / ...
  http://www.biomedsearch.com/nih/Use-lysozyme-nisin-EDTA-combined/20492308.html
*  Growth medium - Wikipedia
An agar plate - an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are ... Maintain the viability of all organisms in the specimen without altering their concentration ... Blood-free, charcoal-based selective medium agar (CSM) for isolation of Campylobacter ... Lack of carbon, nitrogen, and organic growth factors so as to prevent microbial multiplication ...
  https://en.wikipedia.org/wiki/Selective_medium
*  Growth medium - Wikipedia
... and organic growth factors so as to prevent microbial multiplication Transport media used in the isolation of anaerobes must be ... Certain bacterial inhibitors are used for gonococci, and buffered glycerol saline for enteric bacilli. Venkataraman Ramakrishna ... Temporary storage of specimens being transported to the laboratory for cultivation Maintain the viability of all organisms in ... YM (yeast extract, malt extract agar) has a low pH, deterring bacterial growth. MacConkey agar is for Gram-negative bacteria. ...
  https://en.wikipedia.org/wiki/Growth_medium
*  Andrea Rocha Co-authors Article on Soil Metabolites for Frontiers in Microbiology
To evaluate the viability of the SDM, we examined the growth of 30 phylogenetically diverse soil bacterial isolates from the ... that this approach can be expanded to other environments to enhance isolation and characterization of diverse microbial ... Andrea is a Senior Staff Scientist based in Tennessee focused on leading-edge research using an array of geochemical, microbial ... Andrea Rocha (Tennessee) will deliver a presentation entitled "High-throughput Sequencing as a Microbial Diagnostic Tool for ...
  https://www.geosyntec.com/about/news-events/4-news/5994-andrea-rocha-co-authors-article-on-soil-metabolites-for-frontiers-in-microbiology
*  Bacterial strain, processed plant extracts, compositions containing same, processes for their preparation and their...
The microbial biomass could be kept in saline in the refrigerator for 48 hours without loosing viability.. Example 3. ... Isolation and Growth of the Probiotic Organism E. coli ATCC Deposit No. 202226. The probiotic organism E. coli (deposited at ... This latter phenomenon has been describedunder such names as `bacterial antagonism`, `bacterial interference`, `barrier effect ... The bacterial strains identified byany such test are within the category of probiotic bacteria, as the term is used herein.. ...
  http://www.patentgenius.com/patent/6500424.html
*  Enhancement of biofilm-mode growth of CoNS cells by oxa | Open-i
Microbial Viability/drug effects. *Microscopy, Confocal. *Microscopy, Fluorescence/methods. *Polysaccharides, Bacterial/ ... Bottom Line: Epifluorescence microscopy and confocal laser scanning microscopy in combination with bacterial viability staining ... Bottom Line: Epifluorescence microscopy and confocal laser scanning microscopy in combination with bacterial viability staining ... Epifluorescence microscopy and confocal laser scanning microscopy in combination with bacterial viability staining and ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2902406_1476-0711-9-16-1&req=4
*  Enumeration and quantification of M. leprae in mouse FP | Open-i
... viability was markedly decreased by 8 weeks in immunocompetent mice.Rifapentine significantly reduced bacterial viability after ... leprae in tissues without the need for bacterial isolation or immediate processing, making these assays applicable for in vivo ... viability was markedly decreased by 8 weeks in immunocompetent mice.Rifapentine significantly reduced bacterial viability after ... In contrast, viability was markedly decreased by 8 weeks in immunocompetent mice. Rifapentine significantly reduced bacterial ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC3750008_pntd.0002404.g001&req=4
*  Resilience of microbial communities in a simulated drinking water distribution system subjected to disturbances: role of...
... maintaining microbial water quality) of the systems.19 It also provides a window into the bacterial genome structure and the ... Microbial Cell Viability Assay kit on a GloMax® 96 microplate luminometer (Promega, Madison, WI), following the protocol by ... Genomic DNA was extracted using an UltraClean DNA Microbial Isolation kit (MoBio Laboratories, Solana Beach, CA). Genome ... DNA Isolation Kit. Total DNA from BW was extracted using a MoBio PowerWater® DNA Isolation Kit. DNA extractions followed the ...
  http://pubs.rsc.org/en/content/articlehtml/2016/ew/c6ew00053c?page=search
*  Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea
Although bacterial isolation is considered the gold standard for diagnosing bacterial disease, the isolation of mycobacteria ... and isolation can be adversely affected by other microbial contamination (Pate et al., 2005). Instead, mycobacteria have been ... from paradise fish, as freezing the spleens and kidneys affected the viability of the mycobacteria, resulting in negative ... Histopathological examination led us to suspect mycobacteriosis, so we attempted bacterial isolation using Middlebrook 7H10 ...
  http://www.kpubs.org/article/articleMain.kpubs?articleANo=E1HKAL_2013_v16n3_165
*  Listeria - The Full Wiki
Bacterial adherence and viability on cutting board surfaces. Journal of Food Safety. 14: 153-172. ... Microbial attachment to food and food contact surfaces. In: Advances in Food and Nutrition Research, Vol. 43. ed. Taylor, S.L. ... Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review. FEMS Microbiology Reviews. ... Refrigerated foods in the home should be kept below 4C to discourage bacterial growth.[9] preventing listeria also can be done ...
  http://www.thefullwiki.org/Listeria
*  Resetting microbiota by Lactobacillus reuteri inhibits T reg deficiency-induced autoimmunity via adenosine A2A receptors | JEM
... our results also showed reduced gut microbial diversity and altered bacterial composition in T reg cell-deficient SF mice, ... Naive CD4+ T cell isolation and in vitro differentiation to Th1 and Th2. Naive CD4+ T cells were isolated from the spleens of 6 ... After 96 h, cell viability was measured by TACS XTT cell proliferation assay kit (Trevigen, Inc.). ... Gut microbial Shannon diversity analysis (two-way ANOVA; n = 6-12 per group). (D) Gut microbial analysis of weighted unifrac- ...
  http://jem.rupress.org/content/early/2016/12/22/jem.20160961
*  Lipid Metabolism sub-cluster 73
Column fractionation and purification of an n-hexane fraction led to the isolation of three lupeol-based triterpenoid esters ... New chemical-enzymatic technology based on the modification of the bacterial polysaccharide K5 from Escherichia coli leads to ... Prebiotic Fructooligosaccharides (FOS) escape metabolism in upper GI tract undergo microbial metabolism in colon and thereby ... In vitro fermentation of prebiotics by Lactobacillus plantarum CFR 2194: selectivity, viability ... ...
  http://www.biomedsearch.com/cluster/14/Lipid-Metabolism/sub-73-p10.html
*  A microbial world within us - Zoetendal - 2006 - Molecular Microbiology - Wiley Online Library
The host genotype affects the bacterial community in the human gastrointestinal tract. Microbial Ecol Health Dis 13: 129-134. * ... Isolation and characterization of GI tract microbes have given us insight into their physiology and potential roles in the GI ... Viability and metabolic activity. While a first step in studying ecosystems is the identification of their members, a ... Construction and analysis of bacterial artificial chromosome libraries from marine microbial assemblage. Environ Microbiol 2: ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05056.x/full
*  ASMscience | Community Structure: Bac
The PCR methods have been used with samples from sediments and microbial mats, as well as deep-sea holothurian guts, often ... showing unexpected bacterial and archaeal diversity. Some studies have used information about the 16S rRNA clones short of ... The oldest method for obtaining information on microbial community structure is to examine the sample with a microscope and ... Button, D. K.,, F. Schuts,, P. Quang,, R. Martin, and, B. R. Robertson. 1993. Viability and isolation of marine bacteria by ...
  http://www.asmscience.org/content/book/10.1128/9781555815882.ch35
*  Metagenomic Analysis of Stress Genes in Microbial Mat Communities from Antarctica and the High Arctic
Analysis of bacterial exopolysaccharides. Biotechnol. Appl. Biochem. 9:12-19 [PubMed]. 23. Klein W, Weber MHW, Marahiel MA. ... Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol. 36:251-258 ... Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures. ... Recent developments in bacterial cold-shock response. Curr. Issues Mol. Biol. 6:125-136 [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3255749/?lang=en-ca
*  Burkholderia pseudomallei - Wikipedia
... viability, and isolation from contaminated specimens". J Bacteriol. 55 (1): 115-126. PMC 518415 . PMID 16561426. Wuthiekanun V ... March 2007). "Suitability of partial 16S ribosomal RNA gene sequence analysis for the identification of dangerous bacterial ... Microbial Pathogenesis. 36 (5): 287-9. doi:10.1016/j.micpath.2004.01.001. PMID 15043863. Nandi T, Ong C, Singh AP, Boddey J, ... Rose, L. J.; O'Connell, H. (2009-05-01). "UV Light Inactivation of Bacterial Biothreat Agents". Applied and Environmental ...
  https://en.wikipedia.org/wiki/Burkholderia_pseudomallei
*  Genetics - Medical Microbiology - NCBI Bookshelf
These latter characteristics are often selected as the inherited traits to be analyzed in studies of bacterial genetics. ... Bacterial viruses (bacteriophages or phages) have DNA or RNA as genetic material. The two essential functions of genetic ... Plasmids usually encode traits that are not essential for bacterial viability, and replicate independently of the chromosome. ... Isolation of lactose-negative (phenotype Lac-) mutants of E. coli poses a different problem. On minimal media with lactose as ...
  https://www.ncbi.nlm.nih.gov/books/NBK7908/?report=reader
*  Robotic Instruments Products, Reviews and Suppliers on SelectScience
QUANTOM Tx™ Microbial Cell Counter. Logos Biosystems, Inc.. Single bacterial cell detection - Rapid quantification - No ... Cellometer Auto 2000 Cell Viability Counter. Nexcelom Bioscience. All-in-one, touch-screen instrument for viability of primary ... DNA / RNA Isolation Purification * Evidence Collection & Analysis * Light Microscopy * Toxicology / Drugs of Abuse Testing ... The LUNA-FL™ can even detect and accurately measure the count and viability of primary cells such as PBMCs, splenocytes, ...
  http://www.selectscience.net/robotic-instruments/product-directory/?u=AC3B4D5D-08BB-44AD-98FD-5E42BCB6F7A6
*  Human presence impacts fungal diversity of inflated lunar/Mars analog habitat | Microbiome | Full Text
Treatment of samples with propidium monoazide (PMA; a DNA intercalating dye for selective detection of viable microbial ... populations) had a significant effect on the microbial diversity compared to non-PMA-treated samples. Statistical analysis ... and immunological characteristics of human inhabiting in isolation, but it was also available for other studies such as ... Bacterial diversity in two neonatal intensive care units (NICUs). PLoS One. 2013;8(1):e54703.PubMedPubMed CentralView Article ...
  https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0280-8
*  Lipopolysaccharide Stimulates p62-Dependent Autophagy-Like Aggregate Clearance in Hepatocytes
Hepatocyte isolation from mice was performed as previously described [18, 26]. Hepatocyte purity was assessed to be ,99% by ... Y. Vodovotz, S. Liu, C. McCloskey, R. Shapiro, A. Green, and T. R. Billiar, "The hepatocyte as a microbial product-responsive ... F. Randow, "How cells deploy ubiquitin and autophagy to defend their cytosol from bacterial invasion," Autophagy, vol. 7, no. 3 ... flow cytometry with a typical viability of over 95% using trypan blue staining. Hepatocytes (150,000 cells/mL) were plated ...
  https://www.hindawi.com/journals/bmri/2014/267350/
*  Frontiers | Parasitic Nematodes Exert Antimicrobial Activity and Benefit From Microbiota-Driven Support for Host Immune...
Our data suggest that nematodes sense their microbial surrounding and have evolved factors that limit the outgrowth of certain ... Our data suggest that nematodes sense their microbial surrounding and have evolved factors that limit the outgrowth of certain ... senses its microbial environment and if the anti-nematode immune and regulatory responses are altered in mice devoid of gut ... senses its microbial environment and if the anti-nematode immune and regulatory responses are altered in mice devoid of gut ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2018.02282/full
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Immunology, Issue 86, Gram-Positive Bacterial Infections, Pneumonia, Bacterial, Lung Diseases, Respiratory Tract Infections, ... Biofilm cell viability is assayed after the recovery period. The MBC-P for the antimicrobial agent is defined as the lowest ... The microbial community encased within the biofilm often shows the differentiation of distinct subpopulation of specialized ... In nature, bacteria rarely exist in isolation; they are instead surrounded by a diverse array of other microorganisms that ...
  https://www.jove.com/visualize/abstract/21187931/prophage-spontaneous-activation-promotes-dna-release-enhancing