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*  Sensors | Free Full-Text | The Evolution of the Bacterial Luciferase Gene Cassette (lux) as a Real-Time Bioreporter | HTML
While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in ... The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to ... Shiga toxin expression in E. coli. Gene expression profiling in enterohemorrhagic E. coli. [64]. ... Construction of a novel bioluminescent reporter system for investigating Shiga toxin expression of enterohemorrhagic ...
  http://www.mdpi.com/1424-8220/12/1/732/htm
*  Frontiers | Simulation Study of cDNA Dataset to Investigate Possible Association of Differentially Expressed Genes of Human...
... is a family of structurally and functionally related bacterial cytotoxins produced by Shigella dysenteriae serotype 1 and ... is a family of structurally and functionally related bacterial cytotoxins produced by Shigella dysenteriae serotype 1 and ... The functional enrichment analysis showed 6 down-regulated and 1 up-regulated genes. Among these DEGs, IL6 was associated with ... Shiga toxins (Stxs) are bacterial cytotoxins mainly produced by enteric pathogens. It is a family of related toxins with two ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2018.00380/full
*  Sub-Optimal Treatment of Bacterial Biofilms
Dutta S., Iida K., Takade A., Meno Y., Nair G.B., Yoshida S. Release of shiga toxin by membrane vesicles in shigella ... Gerdes K., Maisonneuve E. Bacterial persistence and toxin-antitoxin loci. Annu. Rev. Microbiol. 2012;66:103-123. doi: 10.1146/ ... from initial bacterial adhesion to maintenance of the biofilm's structural integrity. eDNA adsorbs to the surface of bacterial ... Sub-Optimal Treatment of Bacterial Biofilms. Tianyan Song,1,2,3,† Marylise Duperthuy,1,2,† and Sun Nyunt Wai1,2,* ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC4929437/
*  Toxins | Free Full-Text | Shiga Toxin: Expression, Distribution, and Its Role in the Environment | HTML
Shiga toxin in the environment to work that demonstrates that the genetic mechanisms underlying the production of Shiga toxin ... Specifically, we review studies that offer an expanded view of environmental reservoirs for Shiga toxin producing microbes in ... we highlight recent work that has increased our understanding of the production and distribution of Shiga toxin in the ... Toxin gene expression by Shiga toxin-producing Escherichia coli: The role of antibiotics and the bacterial SOS response. Emerg ...
  http://www.mdpi.com/2072-6651/3/6/608/htm
*  Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks | BMC Microbiology | Full Text
These data could indicate that Shiga toxins are experiencing purifying selection. ... Stx1 and Stx2 are genetically and immunologically distinct but share the same compound toxin structure, method of entry and ... Shiga toxins 1 and 2 (Stx1 and Stx2) are bacteriophage-encoded proteins that have been associated with hemorrhagic colitis, ... The toxin has also been observed in other bacterial genera, including Citrobacter, Enterobacter and Acinetobacter [9-11]. ...
  https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-7-109
*  Acute Gastrointestinal Infections - Bacterial Infections Part 1 | Coursera
the production of shiga-like toxin, as well as new bacteriophages.. Once ingested by humans the StEC bacterium displays ... The Shiga-like toxin can then enter these blood vessels causing endophilio ... Bacterial Infections Part 1. In Module Two, we will explore six infectious diseases caused by bacteria. We will see how factors ... There's also evidence to suggest that the genes for toxin production. are upregulated in bacteria that sense their imminent ...
  https://es.coursera.org/lecture/stories-of-infection/acute-gastrointestinal-infections-xBBSc
*  Fairview Diagnostic Laboratories
Shiga Toxin 1 & 2 genes (Shiga toxin producing E.coli typically harbors one or both genes) ... Culture for bacterial pathogens will be performed if PCR is invalid. Turnaround Time:. Performed daily; results are reported ... Stool Pathogens; Shiga Toxin 1 & 2; Salmonella; Shigella; Campylobacter; Yersinia enterocolitica; Vibrio; Norovirus; Rotavirus ... Salmonella, Shigella and Campylobacter, Shiga Toxin 1&2, Yersinia enterocolitica, Vibrio, Norovirus and Rotavirus. ...
  http://labguide.fairview.org/showtest.asp?testid=6389&format=long
*  Typical and Atypical Enteropathogenic <em>Escherichia coli</em> - Volume 8, Number 5-May 2002 - Emerging Infectious Diseases...
Atypical EPEC is more closely related to Shiga toxin-producing E. coli (STEC), and like STEC these strains appear to be ... Non production of Shiga-like toxins by Escherichia coli serogroup O26. Rev Microbiol, São Paulo. 1994;25:154-5. ... data) and the afa gene of serotype O55:H7 are located on the bacterial chromosome (R. Keller et al., unpub. data). Typical and ... Epidemiology of Shiga toxin-producing Escherichia coli infections in humans in the United States. In: Kaper JB, editor. ...
  https://wwwnc.cdc.gov/eid/article/8/5/01-0385_article
*  Browsing by Subject "Bacteriophages -- Research"
Human infections with Shiga-toxin (Stx)-producing E. coli (STEC) vary in severity of illness. The pan-genome of a bacterial ... The evolution of the pan-genome of Shiga-toxin (Stx) producing Escherichia coli and the Stx₂ bacteriophage  ... Mannheimia haemolytica is the principal bacterial agent associated with bovine respiratory disease (BRD). It has a significant ...
  https://www.uleth.ca/dspace/browse?value=Bacteriophages+--+Research&type=subject
*  David I. Friedman, M.D. | Microbiology & Immunology | Michigan Medicine | University of Michigan
The operator and early promoter region of the Shiga toxin type 2-encoding bacteriophage 933W and control of toxin expression. J ... Host-Virus interactions in regulation of gene expression and viral contribution to bacterial pathogenesis. This laboratory uses ... Shiga-like toxin: One serious public health problem that is of current concern is the infections caused by certain Escherichia ... The major contributor to the virulence of these bacteria are the products of genes encoding Shiga toxin (Stx), a glucosidase ...
  https://medicine.umich.edu/dept/microbiology-immunology/david-i-friedman-md
*  Shiga toxin 1 targets bovine leukemia virus-expressing cells. - Semantic Scholar
The active A subunit of Stx type 1 (StxA1) targeted a selected population of permeable cells expressing BLV and inhibited BLV ... Direct evidence that Escherichia coli Shiga toxin (Stx) acts against bovine leukemia virus (BLV)-expressing cells was obtained ... ecf operon and lipid A myristoyl transferase activity in bacterial survival in the bovine gastrointestinal tract and bacterial ... Shiga toxin 1 targets bovine leukemia virus-expressing cells.. *. Witold A. Ferens. , Luke J. Grauke. , Carolyn J. Hovde ...
  https://www.semanticscholar.org/paper/Shiga-toxin-1-targets-bovine-leukemia-virus-expres-Ferens-Grauke/5943c9247800493821b88ae8bb7ddd7309b5389c
*  Frontiers | Screening of an E. coli O157:H7 Bacterial Artificial Chromosome Library by Comparative Genomic Hybridization to...
A bacterial artificial chromosome (BAC) library was generated from a derivative of the sequenced E. coli O157:H7 Sakai strain. ... A bacterial artificial chromosome (BAC) library was generated from a derivative of the sequenced E. coli O157:H7 Sakai strain. ... and Shiga toxins (Hayashi et al., 2001; Perna et al., 2001; Ogura et al., 2009). Therefore these often large, horizontally ... Bacterial Strains, Plasmids, Oligonucleotides and Media. The bacterial strains and plasmids used in the study are described in ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2011.00168/full
*  Incidence and Trends of Infection with Pathogens Transmitted Commonly Through Food - Foodborne Diseases Active Surveillance...
Shiga toxin-producing Escherichia coli.. † The position of each line indicates the relative change in the incidence of that ... Number of cases of culture-confirmed bacterial and laboratory-confirmed parasitic infection, hospitalizations, and deaths, by ... Abbreviations: CI = confidence interval; STEC = Shiga toxin-producing Escherichia coli. * No significant change = 95% CI is ... The incidences of infection caused by Campylobacter, Salmonella, Shiga toxin-producing Escherichia coli O157, and Vibrio are ...
  https://www.cdc.gov/mmwr/preview/mmwrhtml/mm6315a3.htm
*  Frontiers | Calpain Mediates Epithelial Cell Microvillar Effacement by Enterohemorrhagic Escherichia Coli | Microbiology
HUS, the leading cause of renal failure in children in the US, is caused by systemic absorption of the EHEC toxin, Shiga toxin ... Bacterial Strains. Table 1 lists bacterial strains used in this study. The wild type, Stx-deficient EHEC strain of serotype ... Tarr, P. I., Gordon, C. A., and Chandler, W. L. (2005). Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome ... The lack of Shigatoxin production by TUV-93-0 permits the prolonged infection of a wide variety of cell lines without the ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2011.00222/full
*  Environmental factor that causes ill health - Answers on HealthTap
Botulinum toxin, shiga toxin, tetnus toxin, and diptheria toxin are the most potent poisons known (ok, except for uranium and ... First: Bacterial endotoxins ( see http://textbookofbacteriology.Net/endotoxin.Html ) and exotoxins ( http://en.Wikipedia.Org/ ... Are there certain bacterial toxins that play a more important role in sepsis? ... Botulinum toxin, shiga toxin, tetnus toxin, and diptheria toxin are the most potent poisons known (ok, except for uranium and ...
  https://www.healthtap.com/topics/environmental-factor-that-causes-ill-health
*  Diarrhea | ARUPConsult Lab Test Selection
If an isolate requires testing for Shiga-like toxin (eg, STEC), refer to E. coli Shiga-like toxin by EIA ... Bacterial diarrhea represents only 1-5% of diarrhea cases and is often associated with clustering of cases or outbreaks. ... Testing includes cultures for Salmonella, Shigella, Campylobacter, E. coli O157, and EIA for Shiga-like toxin from E. coli ... If systemic symptoms of acute salmonellosis are present, the preferred tests are stool culture and E. coli Shiga-like toxin by ...
  https://arupconsult.com/content/diarrhea
*  Ribosome-inactivating protein, subdomain 1 (IPR016138) | InterPro | EMBL-EBI
A number of bacterial and plant toxins act by inhibiting protein synthesis in eukaryotic cells. The toxins of the shiga and ... Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N- ... Members of the family include shiga and shiga-like toxins, and type I (e.g. trichosanthin and luffin) and type II (e.g. ricin, ... Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I.. Proc. Natl. Acad. Sci. U.S.A. 85 2568-72 1988 ...
  http://www.ebi.ac.uk/interpro/entry/IPR016138
*  The Journal of General and Applied Microbiology
The Shiga toxin (Stx)-encoding phage plays an important role in the horizontal transfer of the stx gene. However, the influence ... Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH ... Bacteriophages are genetic elements that play key roles in the evolution and diversification of bacterial genomes. ... The amino acid sequence of XynF1 had 99% coverage and 95% homology to the endo-β-1,4-xylanase encoded by the cellulase gene of ...
  https://www.jstage.jst.go.jp/browse/jgam/64/1/_contents/-char/en
*  Genomics and outbreak investigation: from sequence to consequence | Genome Medicine | Full Text
Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4. N Engl J Med. 2011, 365: 718-724.View ArticlePubMed ... Bench-top sequencing of whole bacterial genomes in near real time to confirm or refute the existence of outbreaks of MRSA or C ... an outbreak of Shiga-toxin-producing Escherichia coli (STEC) struck Germany in May-June 2011, resulting in over 3,000 cases and ... Epidemic profile of shiga-toxin-producing Escherichia coli O104:H4 outbreak in germany - preliminary report. N Engl J Med. 2011 ...
  https://genomemedicine.biomedcentral.com/articles/10.1186/gm440
*  EXAM #1: BACTERIAL INFECTIONS OF THE GI TRACT Flashcards by Jonathan Kallevang | Brainscape
BACTERIAL INFECTIONS OF THE GI TRACT flashcards from Jonathan Kallevang ... Shiga-Like Toxins 1 and 2 i.e. "SLT-I and SLT-II" ... ETEC toxin is similar to the cholera toxin and causes a WATERY ... What is the MOA of the ETEC toxin? What symptom does this toxin cause? ... Where are C. botulinum spores and toxins identifiable in Floppy Baby Syndrome? ...
  https://www.brainscape.com/flashcards/exam-1-bacterial-infections-of-the-gi-tra-4224846/packs/6311361
*  Sensors | Free Full-Text | A High-Throughput Antibody-Based Microarray Typing Platform | HTML
The assay was demonstrated using the Shiga-toxin producing E. coli (STEC), E. coli O157:H7 as well as the "Big Six" non-O157 ... multiplexed detection of foodborne bacterial pathogens and toxins [7-9]. In this study, the high capacity of microarray to ... To that end, two sets of antibodies raised against each of the "Big Six" non-O157 Shiga toxin-producing E. coli (STEC) as well ... Anti-Shiga toxin-1 (Stx-1) antibody (from Toxin Technology, Sarasota, FL, USA) was labeled with Alexa Fluor 555 (from ...
  http://www.mdpi.com/1424-8220/13/5/5737/htm
*  M- Bacterial Infections of the Gut Flashcards by heather Fahey | Brainscape
Bacterial Infections of the Gut flashcards from heather Fahey ... 2. Phage-encoded Shiga toxin binds to G3 glycolipid receptor on ... M- Bacterial Infections of the Gut Flashcards Preview GI, Liver, GallBladder, Pancreas , M- Bacterial Infections of the Gut , ... ETEC has a heat labile (LT) and heat stabile (ST) toxin. LT is an ab toxin like cholera. B binds to GM1 ganglioside receptor ... 3. Secrete cholera toxin (A-B with one A and five B) 4. the five B form a pentamer and bind to GM1 ganglioside. 5. the A ...
  https://www.brainscape.com/flashcards/m-bacterial-infections-of-the-gut-1739836/packs/3239092
*  Bacterial genome sequencing in the clinic: bioinformatic challenges and solutions - Semantic Scholar
The potential of bacterial whole-genome sequencing (WGS) to complement existing diagnostic infrastructures in clinical ... Molecular characterization and phylogeny of Shiga toxin-producing E . coli ( STEC ) isolates obtained from two Dutch regions ... The potential of bacterial whole-genome sequencing (WGS) to complement existing diagnostic infrastructures in clinical ... Bacterial genome sequencing in the clinic: bioinformatic challenges and solutions. @article{Fricke2014BacterialGS, title={ ...
  https://www.semanticscholar.org/paper/Bacterial-genome-sequencing-in-the-clinic%3A-and-Fricke-Rasko/b14a506914871eb43c1ddb7fae197a12d0a5b21e
*  Shiga toxins even when different are encoded at identical positions in the genomes of related temperate bacteriophages -...
... expression of Stx and for developing universal diagnostic primers for use in tracking the origin and evolution of Shiga toxins ... It is concluded that toxin expression is directly associated with the initiation of cell lysis. Downstream of the stx 2 operon ... In phage 933W, a region of ORFs interlinked by overlapping start-stop codons (ATGA) was detected preceding the toxin gene. ... appeared to be common to both stx 1 - and stx 2 -harbouring phages. However, only seven of the 22 STEC strains investigated ...
  https://www.semanticscholar.org/paper/Shiga-toxins-even-when-different-are-encoded-at-in-Karch-Schmidt/8ad70103869f68b5f91abab9e18e319207f1b153
*  Robert Hnasko : USDA ARS
Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices. Toxins. Toxins 7: ... Detection of bacterial toxins by lateral flow immunoassay - Ching, K.H., Stanker, L.H., He, X., Hnasko, R.M. 2013. Detection of ... Detection of shiga toxins by lateral flow assay. Toxins. 7:1163-1173. doi: 10.3390/Toxins7041163. ... Detection of shiga toxins by lateral flow assay - Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Mcgarvey, J.A., Hnasko, R.M. ...
  https://www.ars.usda.gov/people-locations/person/?person-id=39021&pf=1