Avian Sarcoma Virus Transformed Hamster Cells made Resistant to Ethidium Bromide | Journal of Cell Science
Hamster cells transformed with the Schmidt-Ruppin strain of avian sarcoma virus were selected for resistance to ethidium bromide (EB). The resistant cell lines proliferated in the presence of up to 30 µg/ml EB.. From avian sarcoma virus-transformed hamster cells already resistant to bromodeoxy-uridine (BrdU), ethidium bromide-resistant cells which were able to grow in 10 µg/ml EB were also prepared. These cells remain deficient in thymidine kinase activity and are suitable for selective preparation of hybrid cells.. The EB resistance was genetically stable. The EB-resistant cell lines, and doubly resistant cells (BrdU, EB) showed no differences in mitochondrial ultrastructure compared with the original cell lines. Thymidine incorporation into mitochondrial DNA was not influenced by EB resistance.. All resistant cell lines, including the ...http://jcs.biologists.org/content/16/3/603
Heteroduplex Analysis of Avian RNA Tumor Viruses - CaltechAUTHORS
Electron microscopic heteroduplex analysis of avian RNA tumor viruses has been undertaken by using 35S viral RNA and long, complementary DNA synthesized in vitro. In this initial study, heteroduplex molecules were formed between complementary DNA from Rous sarcoma virus [Prague B strain (Pr-B)] and RNAs from Pr-B and Rous sarcoma virus [Prague C strain (Pr-C)] and from their transformation defective (td) derivatives, td-Pr-B and td-Pr-C. In the case of heteroduplexes with the td viruses, a deletion loop was observed of the order of two kilobases in size and less than one kilobase from the 3' terminus of the RNA. This deletion probably spans part or all of the sequences of one or more genes in the nondefective sarcoma ...http://authors.library.caltech.edu/4684/
PRCII, a representative of a new class of avian sarcoma viruses - Enlighten: Publications
The Poultry Research Center Virus II (PRC II) is a replication-defective avian sarcoma virus with envelope determinants of the A and B subgroups. In nonproducing cells transformed by PRCII the products of the replicative genes gag, pol, and env are not demonstrable, but a single polyprotein of Mr 105,000 (p105) can be detected. P105 contains peptides of the gag proteins p19 and p27 plus transformationspecific sequences. It does not contain peptides of gPr95env of Pr180gag-PoI (with the possible exception of one pol peptide). The transformation-specific sequences of p105 are distinct form those of p100 of avian carcinoma virus MH2, of p1 10 coded for by avian myelocytoma virus MC29, and of p75 or p40 of avian erythroblastosis virus AEV. They ...http://eprints.gla.ac.uk/123610/
Veterinary PCR Kits - ALV/RSV - Avian leukosis virus Rous sarcoma virus RT-PCR Detection Kit
Avian Lymphoid leukosis has been the most common form of the leukosis/sarcoma group of diseases seen in chicken flocks. Members of the leukosis/sarcoma group of avian retroviruses, have similar physical and molecular characteristics and share a common group-specific antigen. Avian leukosis occurs naturally only in chickens. Experimentally, some of the viruses of the leukosis/ sarcoma group can infect and produce tumors in other species of birds or even mammals. VetPCR ALV/RSV Detection Kit is the direct detection of Avian leukosis virus/Rous sarcoma virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify ...http://www.kitpcr.com/product2/261-ALV_RSV
"Nonmajor histocompatibility complex alloantigen effects on the fate of" by K. T. Lepage, W. E. Briles et al.
Rous sarcoma virus-induced tumor outcome is controlled by the MHC (B). Additional data, using controlled segregation in families, has indicated non-MHC effects as well, but few studies have focused on blood groups other than the B complex. Segregating combinations of genes encoding erythocyte (Ea) alloantigen systems A, C, D, E, H, I, P, and L in (BB5)-B-2 and (BB5)-B-5 MHC (B) backgrounds were examined for their effects on Rous sarcomas. Six-week-old chickens were inoculated in the wing-web with 30 pfu of Rous sarcoma virus (RSV). Tumors were scored six times over a 10-wk period. A tumor profile index (TPI) was assigned to each chicken based on the six tumor size scores. Response was evaluated using tumor size at each measurement period, TPI, and mortality. The genotypes of Ea systems A, C, ...http://scholars.unh.edu/nhaes/178/
Woolly monkey sarcoma virus - Wikipedia
Woolly monkey sarcoma virus (WMSV), with synonym Simian sarcoma virus (often abbreviated by SSV, but this may also stand for some species called 'Sulfolobus spindle-shaped virus', that belong to different genera in family Fuselloviridae) is a species of gammaretrovirus that infects primates. First isolation was from a fibrosarcoma in a woolly monkey (genus Lagothrix). For its reproduction the virus needs a helper or associated virus which is called Simian sarcoma associated virus (SSAV). International Committee on Taxonomy of Viruses NCBI Taxonomy Browser Zoologix Simian ...https://en.wikipedia.org/wiki/Woolly_monkey_sarcoma_virus
AID 550465 - Antiviral activity against Rous sarcoma virus assessed as inhibition of capsid assembly - PubChem
BioAssay record AID 550465 submitted by ChEMBL: Antiviral activity against Rous sarcoma virus assessed as inhibition of capsid assembly.https://pubchem.ncbi.nlm.nih.gov/bioassay/550465
A noncatalytic domain conserved among cytoplasmic protein-tyrosine ki" by I Sadowski, J C. Stone et al.
Proteins encoded by oncogenes such as v-fps/fes, v-src, v-yes, v-abl, and v-fgr are cytoplasmic protein tyrosine kinases which, unlike transmembrane receptors, are localized to the inside of the cell. These proteins possess two contiguous regions of sequence identity: a C-terminal catalytic domain of 260 residues with homology to other tyrosine-specific and serine-threonine-specific protein kinases, and a unique domain of approximately 100 residues which is located N terminal to the kinase region and is absent from kinases that span the plasma membrane. In-frame linker insertion mutations in Fujinami avian sarcoma virus which introduced dipeptide insertions into the most stringently conserved segment of this N-terminal domain in P130gag-fps impaired the ability of Fujinami avian sarcoma virus to transform rat-2 cells. The ...https://mouseion.jax.org/stfb1980_1989/789/
Human immunodeficiency virus tat-activated expression of poliovirus protein 2A inhibits mRNA translation - CaltechAUTHORS
To study the effect of poliovirus protein 2A on cellular RNA translation, the tat control system of human immunodeficiency virus (HIV) was used. Protein 2A was expressed from a plasmid construct (pHIV/2A) incorporating the HIV long terminal repeat. Protein synthesis was measured by using chloramphenicol acetyltransferase as a reporter gene driven by the Rous sarcoma virus long terminal repeat. When HIV/2A was contransfected with the reporter, addition of a tat-producing plasmid caused at least a 50-fold drop in chloramphenicol acetyltransferase synthesis. A HeLa cell line carrying HIV/2A was established. In it, tat expression caused more than a 10-fold drop in chloramphenicol acetyltransferase synthesis from the reporter plasmid. Furthermore, 2A induction by tat caused cleavage of the cellular translation factor P220, a part of eukaryotic translation ...http://authors.library.caltech.edu/7714/
A line of normal rat embryo fibroblasts was transformed with N-methyl-N′-nitro-N-nitrosoguanidine (a chemical carcinogen), SV40 and polyoma virus (two DNA viruses), and Rous sarcoma virus (an RNA tumor virus). In this study, we report a comparison of the levels of collagen synthesis and procollagen messenger RNA (mRNA) in 13 lines selected after transformation with one of these agents. Collagen synthesis and procollagen mRNA levels were compared with the degree of transformation determined from morphology, saturation density, growth in agarose, and tumorigenicity in nude mice. Each class of transformants had a characteristic level of collagen synthesis; this level correlated inversely with the degree of transformation of the rat embryo fibroblasts. In N-methyl-N′-nitro-N-nitrosoguanidine and SV40 transformants which were ...http://cancerres.aacrjournals.org/content/41/3/830
RCSB PDB - 1D1D: NMR SOLUTION STRUCTURE OF THE CAPSID PROTEIN FROM ROUS SARCOMA VIRUS Macromolecule Annotations Page
1D1D: Solution structure and dynamics of the Rous sarcoma virus capsid protein and comparison with capsid proteins of other retroviruses.http://www.rcsb.org/pdb/explore/derivedData.do?structureId=1D1D
RCSB PDB - 1D1D: NMR SOLUTION STRUCTURE OF THE CAPSID PROTEIN FROM ROUS SARCOMA VIRUS Structure Summary Page
1D1D: Solution structure and dynamics of the Rous sarcoma virus capsid protein and comparison with capsid proteins of other retroviruses.http://www.rcsb.org/pdb/explore/explore.do?structureId=1D1D
V-Src - Wikipedia
v-Src is a gene found in Rous sarcoma virus that encodes a tyrosine kinase that causes a type of cancer in chickens. The src gene is oncogenic as it triggers uncontrolled growth in abnormal host cells. It was the first retroviral oncogene to be discovered. The src gene was taken up by RSV and incorporated into its genome conferring it with the advantage of being able to stimulate uncontrolled mitosis of host cells, providing abundant cells for fresh infection. The src gene is not essential for RSV proliferation but it greatly increases virulence when present. Francis Peyton Rous first proposed that viruses can cause cancer. He proved it in 1911 and was later awarded the Nobel prize in 1966. Chickens grow a tumor called a fibrosarcoma. Rous collected and ground up these sarcomas, and then ...https://en.wikipedia.org/wiki/V-Src
anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) (pTyr416) antibody ABIN204748 from antibodies...
anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) (pTyr416) antibody ABIN204748 from antibodies-onlinehttp://www.biocompare.com/9776-Antibodies/4074802-anti-V-Src-Sarcoma-Schmidt-Ruppin-A-2-Viral-Oncogene-Homolog-Avian-SRC-pTyr416-antibody/
anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) antibody (Alexa Fluor 488) ABIN916357 from...
anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) antibody (Alexa Fluor 488) ABIN916357 from antibodies-onlinehttp://www.biocompare.com/9776-Antibodies/4058703-anti-V-Src-Sarcoma-Schmidt-Ruppin-A-2-Viral-Oncogene-Homolog-Avian-SRC-antibody-Alexa-Fluor-488/
The Age Factor in Adaptability of a Sarcoma Virus to Other Animal Species | Science
The age of the chicken in which the Rous sarcoma is grown has an influence on the variation and subsequent adaptation of the causative virus to ducks. Adaptation is relatively easy to accomplish when the tumor has been grown in adult chickens several months of age. It has never been accomplished when the tumor has been grown in chicks and only occasionally when it has been grown in old chickens. ...http://science.sciencemag.org/content/103/2687/748
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The RCAS System Viral Replication and Behavior
click on the image for a larger view) Figure 2. RSV virion structure and expression. Panel A shows a diagrammatic representation of a mature Rous sarcoma virus (RSV) virion. The legend at the right identifies individual proteins found in the mature virion. The outer membrane of the virion contains the transmembrane protein (TM), which is associated with the surface protein (SU). The matrix protein (MA) lies just under this outer membrane. The core of the virion is structurally delimited by the capsid protein (CA). Inside the capsid are two viral RNA genomes, shown partially covered with nucleocapsid protein (NC). The two genomic RNAs are hydrogen bonded near their 5' ends. The core also contains reverse transcriptase (RT), integrase (IN), and protease (PR). Panel B shows the relationship of the proviral DNA, the open reading frames, viral RNAs, and proteins of RSV. The LTRs of the provirus ...https://home.ncifcrf.gov/hivdrp/RCAS/replication.html
Ostelli Plus Prague, Prague, Repúbblica Ceca - Gomio.com
Prenota il ostello Plus Prague a Prague online per il prezzo più economico. Guarda le foto di Plus Prague, legge le critiche e prenota senza pagare nessuna tassa di prenotazione para confirmare il tuo alloggio.http://www.gomio.com/it/hostels/europe/czech-republic/prague/Plus-Prague/overview.htm
Prague is the best place to live in ČR | Prague TV - Living Like a Local!
A survey of Czech regions saw the capital still on top, Moving to a new country is an exciting process! Prague.TV's relocation section offers expats all the information you need to make your move to Prague as smooth as possible.https://prague.tv/en/s72/Directory/c212-Relocation/n11769-Prague-is-the-best-place-to-live-in-CR
Zubní Ordinace - Dentist in Prague - WhatClinic.com
Visit Zubní Ordinace in Prague, Czech Republic. See contact details, check prices, read reviews, look at pictures, and get directions. Dentist Praguehttps://www.whatclinic.com/dentists/czech-republic/prague/zubni-ordinace
Simply Hotel (Prague, Czech Republic) - Reviews, Photos & Price Comparison - TripAdvisor
Book Simply Hotel, Prague on TripAdvisor: See traveller reviews, 14 candid photos, and great deals for Simply Hotel, ranked #189 of 365 B&Bs / inns in Prague and rated 3.5 of 5 at TripAdvisor.https://www.tripadvisor.co.uk/Hotel_Review-g274707-d6000541-Reviews-Simply_Hotel-Prague_Bohemia.html
Pressure-Volume Techniques & Analysis Practical Workshop, Prague: Day 1 Registration, Thu, 22 Jun 2017 at 09:00 | Eventbrite
Eventbrite - ADInstruments Limited presents Pressure-Volume Techniques & Analysis Practical Workshop, Prague: Day 1 - Thursday, 22 June 2017 at IKEM. Find event and registration information.https://www.eventbrite.co.uk/e/pressure-volume-techniques-analysis-practical-workshop-prague-day-1-registration-35002390091
Pav Bryan - Long Hilly Sportive Training Plan - 14 Weeks - 115+ Miles, UK Hills - Support Included | cycling Training Plan |...
This plan has been designed by Pav Bryan Bikes Etc Magazines Cycling Guru. Director and Head Coach at Direct Power Coaching and Nutritionally Fit.https://www.trainingpeaks.com/training-plans/cycling/road-cycling/tp-112880/pav-bryan-long-hilly-sportive-training-plan-14-weeks-115-miles-uk-hills-support-included
ChessCube Forums • View topic - Exclusive interview with Bryan - 1 : Before the event
1) Me: Hello Bryan, I hope you are doing good. And, not only making 2 big events on chesscube - now you keep going with another one ' Raising money for Doctors Without Borders ' - What do you say about this ...http://www.chesscube.com/forum/viewtopic.php?f=18&t=3800&view=previous