Electron Microscopic Autoradiography of Rabbit Reticulocytes Active and Inactive in Protein Synthesis | Science
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We have localized muscarinic cholinergic receptors in the rat brainstem by a light microscopic autoradiographic method. Initially, we examined the kinetics and specificity of the binding of radiolabeled quinuclidinyl benzilate ([3H]QNB) in slide-mounted, frozen tissue sections to determine that we were labeling the muscarinic cholinergic receptors as they had been described previously in biochemical pharmacological studies. Subsequently, using optimal binding parameters to obtain high specific to nonspecific ratios, we labeled tissue sections and generated autoradiograms by the apposition of emulsion- coated coverslips. We found high concentrations of autoradiographic grains associated with many nuclei in the brainstem including the superior colliculus, nuclei of the lateral lemniscus, pontine nucleus, parabrachial nucleus, trigeminal nerve nucleus (V), the tegmental nuclei, nucleus of the facial nerve (VII), the locus coeruleus, the medial vestibular nucleus, cochlear ...http://www.jneurosci.org/content/1/2/176
Autoradiograph - Wikipedia
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance. Alternatively, the autoradiograph is also available as a digital image (digital autoradiography), due to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto- prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography or microradiography, in which the sample is X-rayed using an external source. Some autoradiographs can be examined microscopically for localization of silver grains (such as on the interiors or exteriors of cells or organelles) in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine was being ...https://en.wikipedia.org/wiki/Autoradiograph
Double-tracer autoradiographic study of protein synthesis and glucose consumption in rats with focal cerebral ischemia. |...
Double-tracer autoradiographic study of protein synthesis and glucose consumption in rats with focal cerebral ischemia. - T Christensen, T Balchen, T Bruhn, N H Diemerhttp://www.curehunter.com/public/pubmed10555193.do
"An autoradiographic analysis of postnatal cell proliferation in the no" by J C. Blanks and D Bok
Blanks, J C. and Bok, D, "An autoradiographic analysis of postnatal cell proliferation in the normal and degenerative mouse retina." (1977). Subject Strain Bibliography 1977. 3447 ...https://mouseion.jax.org/ssbb1977/3447/
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Abstract Ultrastructural changes induced in Plasmodium falciparum by artemisinin were studied in vitro. Electron microscopic autoradiography was performed on infected erythrocytes that were exposed in vitro to 3H-dihydroartemisinin and 14C-artemisinin. These drugs consistently were located in food vacuoles and mitochondria. Two hours after administration, changes were observed in parasite mitochondria, rough endoplasmic reticulum, and nuclear envelope. At four hours, in addition to the earlier changes, nuclear membranes and, to a lesser extent, some plasma membranes formed myelin figures. In addition, there was a disappearance of ribosomes, and a destruction of food vacuole membranes. These changes may lead to the total disorganization of the parasites. Approximately 30% of the parasites manifested these alterations.http://www.ajtmh.org/content/journals/10.4269/ajtmh.1993.49.485
Regional measurements of 131I-9.2.27 distribution in human melanoma tumors were obtained using quantitative autoradiography. Tumors were removed from patients 72-96 h after they had received an i.v. injection of 9.15 mCi (100 mg) of 131I-9.2.27. The autoradiographic images showed that the radioactivity reaching the tumor was heterogeneously distributed. Areas of relative high and low uptake were selected in each tumor. Regions of high activity contained from 51 to 1371 nCi/g, while areas with low uptake had radioactivity ranging from 12 to 487 nCi/g. The reliability of the autoradiographic measurements was demonstrated by the strong positive correlation with direct tissue sample counting (r = 0.994 P , 0.001). Since comparative immunocytochemistry showed a homogeneous and diffuse staining of target antigen on viable tumor cells, variability of monoclonal antibody uptake within individual tumors was not primarily due to heterogeneity of antigen expression in these cases. ...http://cancerres.aacrjournals.org/content/49/10/2783
α1-Adrenoceptors in Normal and Hyperalgesic Human Skin | Clinical Science
1. Evidence of an adrenergic component of cutaneous hyperalgesia has recently been obtained in animal models of painful peripheral neuropathy. These findings have prompted speculation that an increased density or sensitivity of peripheral α-adrenoceptors contributes to sensory abnormalities and chronic neuropathic pain in conditions such as reflex sympathetic dystrophy. However, it is not known whether α-adrenoceptors are present at the site of nociception, either in hyperalgesic or normal skin.. 2. We used the selective radioligand 125I-hydroxyphenyl-ethyl-aminomethyl-tetralone (HEAT) to label α1-adrenoceptors, and quantitative autoradiography to assess the relative density of these receptors in skin samples from seven normal individuals and from the hyperalgesic and pain-free limbs of five patients with reflex sympathetic dystrophy. The distribution of autoradiographic grains over the epidermis and dermis was investigated in 10 μm serial transverse sections.. 3. α1-Adrenoceptors were ...http://www.clinsci.org/content/91/1/73
"Radioautographic studies on the intracellular distribution of bleomyci" by J Fujimoto
Fujimoto, J, "Radioautographic studies on the intracellular distribution of bleomycin-14c in mouse tumor cells." (1974). Subject Strain Bibliography 1974. 663 ...https://mouseion.jax.org/ssbb1974/663/
Repeated ECS differentially affects rat brain 5-HT1A and 5-HT2A receptor expression. - Department of Psychiatry
We investigated the effect of electroconvulsive shock (ECS), administered five times over 10 days, on 5-HT1A and 5-HT2A receptor mRNA and binding site densities in the rat brain using in situ hybridization histochemistry and quantitative autoradiography. ECS treatment increased 5-HT1A receptor mRNA abundance and binding site densities in the dentate gyrus, but decreased these parameters in the CA3c layer of the hippocampus. No changes in 5-HT1A receptor mRNA and binding sites occurred in other hippocampal subfields, neocortex or raphe nuclei. Repeated ECS was also found to increase 5-HT2A receptor binding site densities in the neocortex and this was accompanied by a non-significant increase in cortical 5-HT2A receptor mRNA abundance. Our study demonstrates that in the rat, repeated ECS produces anatomically and molecularly discrete effects on 5-HT1A and 5-HT2A receptor gene expression. These changes may be relevant to the therapeutic effect of repeated ECS in depression.https://www.psych.ox.ac.uk/publications/349599
Gamma-Aminobutyric Acid Uptake and Metabolism in 'Aplysia dactylomela'
Selected ganglia of Aplysia dactylomela were found to possess a sodium dependent gamma-aminobutyric acid (GABA) uptake system. Tissue to medium ratios as high as 20:1 were calculated in the buccal ganglion after a 45-minute incubation in 6 Micromoles GABA. The metabolism of accumulated GABA was low with 90 percent of the radioactivity remaining as GABA after a 45-minute incubation and 20-minute washout period. The buccal ganglion displayed a sodium dependent, high affinity uptake system with a Km = 52 Micromoles. No net sodium dependent accumulation was found in the pedal ganglion. In the buccal ganglion, light microscope autoradiography indicated that the sites of GABA accumulation were predominantly extraneuronal.http://www.dtic.mil/docs/citations/ADA012639
Comparative anatomical distribution of 5-HT1A receptor mRNA and 5-HT1A binding in rat brain -- a combined in situ hybridisation...
Abstract: The present study examined the comparative distribution of 5-HT1A receptor mRNA and 5-HT1A receptors in rat brain using a combination of in situ hybridisation histochemistry and in vitro receptor autoradiography. 5-HT1A mRNA was visualised using a 910 bp cRNA probe synthesised from a BalI-PvuII fragment of the rat 5-HT1A reetor gene, while 5-HT1A receptors were labelled with the 5-HT1A-selective ligand 8-OH-DPAT. In general terms, there was a complementary distribution of cells expressing 5-HT1A receptor mRNA and 5-HT1A receptor sites. High levels of both 5-HT1A mRNA and 5-HT1A receptors were evident in the hippocampal formation (CA1, CA3, dentate gyrus), entorhinal cortex, and raphe nuclei and lower levels in neocortex and thalamus. Although 5-HT1A mRNA was not expressed in any regions which did not also exhibit 5-HT1A receptors, within both the diagonal band and the medial septal nucleus mRNA levels were proportionately higher than 5-HT1A receptor levels, possibly reflecting receptor ...https://deepblue.lib.umich.edu/handle/2027.42/29092
Synthesis and evaluation of novel α-fluorinated (E)-3-((6-methylpyridin-2-yl)ethynyl)cyclohex-2-enone-O-methyl oxime (ABP688)...
In the search for an optimal fluorine-18-labeled positron emission tomography (PET) radiotracer for imaging metabotropic glutamate receptor subtype 5 (mGluR5), we have prepared a series of five α-fluorinated derivatives based on the ABP688 structural manifold by application of a two-step enolization/NFSI α-fluorination method. Their binding affinities were evaluated in vitro, and the most promising candidate (Z)-16 exhibited a K(i) of 5.7 nM and a clogP value of 2.3. The synthesis of the precursor tosylate (E)-22 revealed a preference for the (E)-configurational isomer (K(i) = 31.2 nM), and successful radiosynthesis afforded (E)-[(18)F]-16 which was used as a model PET tracer to establish plasma and PBS stability. (E)-[(18)F]-16 (K(d) = 70 nM) exhibited excellent specificity for mGluR5 in autoradiographic studies on horizontal rat brain slices in vitro. ...http://www.zora.uzh.ch/id/eprint/71389/
sup|3|/sup|H]WAY-100635 for 5-HT(1A) receptor autoradiography in human brain: A comparison with [|sup|3|/sup|H]8-OH-DPAT and...
WAY-100635 is the first selective, silent 5-HT(1A) (5-hydroxytryptamine(1A), serotonin-1A) receptor antagonist. We have investigated the use of [ 3 H]WAY-100635 as a quantitative autoradiographic ligand in post-mortem human hippocampus, raphe and four cortical regions, and compared it with the 5-HT(1A) receptor agonist, [ 3 H]8-OH-DPAT. Saturation studies showed an average Kd for [ 3 H]WAY-100635 binding in hippocampus of 1.1 nM. The regional and laminar distributions of [ 3 H]WAY-100635 binding and [ 3 H]8-OH-DPAT binding were similar. The density of [ 3 H]WAY-100635 binding sites was 60-70% more than that of [ 3 H]8-OH-DPAT in all areas examined except the cingulate gyrus where it was 165% higher. [ 3 H]WAY-100635 binding was robust and was not affected by the post-mortem interval, freezer storage time or brain pH (agonal state). Using [ 3 H]WAY-100635, we confirmed an increase of 5-HT(1A) receptor binding sites in the frontal cortex in schizophrenia, previously demonstrated with [ 3 ...https://www.ndcn.ox.ac.uk/publications/657648
The quantitation and kinetics of unscheduled (repair) DNA synthesis in ultraviolet-irradiated human skin by automated image...
Grain counting by eye is a tedious and time-consuming technique but one with great potential in cell kinetics and for the study of DNA excision repair activity (unscheduled DNA synthesis or UDS). We have been investigating the levels of UDS in human skin sections exposed in situ to ultraviolet radiation using a short-term incubation in tritiated thymidine and autoradiography and the decline in UDS levels with time (repair kinetics). We have adapted an automated image analysis system automatically to assess the number of grains over epidermal cell nuclei in autoradiographs of sections of epidermis. An excellent correlation was observed between visual counting and machine measurement of the area (in pixels) occupied by silver grains. The levels of UDS declined with time as lesions are progressively repaired. The half time (+/- standard deviation) for the reduction in UDS is 7.25 +/- 0.18 h. The grain counts can be significantly increased by increasing the autoradiographic exposure, by ...http://christie.openrepository.com/christie/handle/10541/95624
Protein Synthesis in the Brain of Patients With Fragile X Syndrome - Full Text View - ClinicalTrials.gov
Biosynthesis of proteins is essential for growth and continued maintenance of the entire neuron including axons, dendrites, and synaptic terminals, and it is clearly one of the important biochemical processes underlying adaptive changes in the nervous system. Studies in experimental animals with the quantitative autoradiographic L [1 (14)C]leucine method have demonstrated a number of the physiological and pathological conditions in which changes in regional rates of cerebral protein synthesis (rCPS) occur.. We have recently developed the first fully quantitative method for determining rCPS with positron emission tomography (PET). The PET method was adapted from the autoradiographic L [1 (14)C]leucine method; it uses L [1 (11)C]leucine as the PET tracer, dynamic scanning, and a kinetic modeling approach for quantification. This method was validated in nonhuman primates by comparison of PET measurements with those based on established biochemical and ...https://clinicaltrials.gov/ct2/show/NCT00362843?recr=Open&cond=%22Fragile+X+Syndrome%22&rank=6
INT6293 - wiki-pain
The distribution of cells expressing mu-receptor mRNA and mu-receptor binding sites were compared in brain and spinal cord tissue sections using a combination of in situ hybridization and receptor autoradiographic techniques. mu-Receptor mRNA was visualized with a 35S-labeled cRNA probe directed to transmembrane III-VI of the rat mu-receptor, while mu-receptor binding sites were labeled with the mu-selective ligand [3H]DAMGO ...http://gnteam.cs.manchester.ac.uk/demos/wiki-pain/vhosts/wikipaints/mediawiki-1.19.1/index.php/INT6293
Alterations in cerebral blood flow and glucose utilization in mice overexpressing the amyloid precursor protein.
Abstract We have used quantitative autoradiographic techniques to study the relationship between cerebral blood flow (CBF) and glucose utilization (CGU) in two lines of transgenic ..https://www.omicsonline.org/references/alterations-in-cerebral-blood-flow-and-glucose-utilization-in-mice-overexpressing-the-amyloid-precursor-protein-1177384.html
Autoradiography - Immune Response - Barnard Health Care
4. Exposure by placing the prepared sample in close approximation with a special photographic emulsion so that the products of radioactive decay in the sample may interact with the silver halide crystals of the emulsion to form latent images.. 5. Developing the latent images to convert them into silver grains.. 6. Fixing the resultant autoradiogram by washing away unreacted silver halide crystals.. The silver grains thus deposited are visualized and their position noted in relation to the underlying sample structure to try and understand the distribution of the label in the sample. Depending upon the nature of the information sought, autoradiography can be carried out at three levels:. 1. Gross level: in the case of samples such as entire organs, large tissue slices, leaves, gels, etc., where the resolution required is of the order perceived by the naked eye* (0.2 mm). 2. Microscopic level: cellular and tissue level localization, resolution of around 1-5 ,xm required.. 3. Ultrastructural level: ...https://www.barnardhealth.us/immune-response/autoradiography.html
Observations and quantitative radioautographic studies of calcium45 deposited in vivo in forming Haversian systems and old bone...
Steven A. Abrams, Nora V. Esteban, Nancy E. Vieira, James B. Sidbury, Bonny L. Specker, Alfred L. Yergey, Developmental changes in calcium kinetics in children assessed using stable isotopes, Journal of Bone and Mineral Research, 1992, 7, 3, ...http://onlinelibrary.wiley.com/doi/10.1002/aja.1000990205/abstract
Comparisons were made by autoradiographic methods of incorporation of tritiated precursors of DNA, RNA, and proteins in C1498 and E.L.4 ascites tumors at various times during the growth of tumors in a susceptible strain of mice (C57BL) and a resistant coisogenic subline (B10.D2), which differ only at the H-2 histocompatibility locus. Autoradiographic examination at various times following tumor inoculation revealed a close similarity in pattern of incorporation of precursors into nucleic acids and proteins of the tumor cells in both sublines until tumor destruction took place in the resistant mice. Only during the period of actual homograft rejection did the extent of labeling of the cells in the resistant line decline greatly. A slight, as yet unexplained, stimulation of incorporation of labeled uridine and amino acids into tumor cells frequently was noted in the resistant animals just prior to tumor rejection.. ...http://cancerres.aacrjournals.org/content/24/10/1798
PCR products were separated by electrophoresis (Protean-2, Biorad, Issy-les-Moulineaux, France) on 8% polyacrylamide gels, which were then vacuum dried and processed for autoradiography on Biomax films (Kodak, Sevran, France). Exposure times were adjusted to avoid film saturation. For each primer pair, we first determined the optimal hybridization temperature and minimum number of PCR cycles necessary to detect radiolabeled reaction products at a unique, expected molecular size, so that each PCR analysis would be performed in the exponential phase of amplification. Forward and reverse primers were designed in separate exons to avoid any bias caused by residual genomic contamination. PCR-amplified products were checked with restriction enzymes. For all primer pairs, no amplification was observed when PCR was performed on either RNase-free water or non-reverse-transcribed RNA samples. At least three independent PCRs were performed on the same samples for each primer pair.. Optical densities (ODs) ...http://www.jneurosci.org/content/23/5/1792
Peter Lipton, Department of Physiology, UW-Madison
The first concerns cellular mechanisms of learning and memory. There is considerable evidence that a crucial step in memory formation is the synthesis of new proteins in the hippocampus. We are using autoradiographic techniques to focus on proteins that are made in dendrites of hippocampal neurons, near synapses. Very few proteins are made there, but we have found out that electrical stimulation of the axons that make contact with these dendrites greatly stimulates their synthesis of proteins. This only happens when the stimulus is combined with another neurotransmitter, acetylcholine. There is reason to believe this phenomenon is important in memory formation and we are studying its mechanisms and significance. The second area concerns the regulation of synaptic transmission by glycolysis. We have found that synaptic transmission in the hippocampus will only occur if glycolysis is active, even if adequate energy is supplied in other ways. This is very unexpected. There appears to be a ...http://www.physiology.wisc.edu/faculty/lipton.html
Sharon Vile on HubPages
I've had a decades-long fascination with herbs and natural healing and found over the years that many such treatments can be quite successful. I'm...https://hubpages.com/@blueheron
Branded car magnets | FASTSIGNS® of Grand Rapids, MI
Generate more awareness for your business with branded car magnets. These versatile magnets offer a less permanent option over decals or wraps.https://www.fastsigns.com/467-grand-rapids-mi/products/product-details/branded-car-magnets