Selective Inhibition of the Lectin Pathway of Complement with Phage Display Selected Peptides against Mannose-Binding Lectin...
The concentration of the free enzyme in the inhibitory assay was determined by the ratio of the residual enzyme activity (in ... The high pathway selectivity of the SFMI inhibitors is somewhat unexpected considering that these are substrate-like inhibitors ... Reagents. All common laboratory reagents were from Sigma-Aldrich (St. Louis, MO) and Merck (Whitehouse, NJ). Maxisorp plates ... The sulfhydryl of the cleaved substrate reacted with the cosubstrate dithiopyridine (143057; Sigma-Aldrich) producing the ...http://www.jimmunol.org/content/185/7/4169
... peptide substrate and/or fluorophore must be examined with secondary assays that use different outputs for sortase activity ( ... Copeland R (2005) Tight binding inhibition, in Evaluation of Enzyme Inhibitors in Drug Discovery: a Guide for Medicinal ... The partial amino acid sequence of the SrtA substrate LPAT fused to cyanoalkene and sulfhydryl moieties allowed for the ... Although poor in inhibition, the usefulness of this reagent was realized when a kinetic analysis of the inhibitory pattern ...http://pharmrev.aspetjournals.org/content/60/1/128
Purification and characterization of α-Amylase from Miswak Salvadora persica | BMC Complementary and Alternative Medicine |...
The enzyme in the toothpaste retained 55% of its original activity after 10 months of storage at room temperature. ... Crude miswak α-amylase in the toothpaste retained 55% of its original activity after 10 months of storage at room temperature. ... amylase activities were almost completely abolished by 100 mM EDTA [32, 43]. For the effect of inhibitors reagents such as PMSF ... One unit of enzyme activity was defined as the amount of enzyme which liberated one μmol maltose per h under standard assay ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-119
Cellular Redox State and Endothelial Dysfunction in Mildly Hyperhomocysteinemic Cystathionine β-Synthase-Deficient Mice |...
GPx-1 activity was monitored by following the decrease in light absorbance at 340 nm with oxidation of NADPH. Enzyme activity ... Thiols were then measured by adding 200 μL Ellman's reagent from a commercially available assay to 50 μL of neutralized ... the reaction product of sulfhydryl groups with 5,5′-dithiobis-2-nitrobenzoic acid (Ellman's reagent). An equal volume of 10% ... Aortic P-selectin was visualized by using the diaminobenzidine substrate method as described in the ImmunoCruz Staining Systems ...http://atvb.ahajournals.org/content/22/1/34
AquaSpark(TM) - Technology - Biosynth Direct Store
... which are ideal for applications in enzyme linked immunosorbent assays (ELISA). ... substrate commercially available for any assay or analytical method based on the detection of alpha-glucosidase activity.. ... Enzymes, Substrates, Reagents, Cofactors. *Enzyme Inhibitors. *Lipids. *Nucleoside, Nucleotide Chemistry. *Protease Inhibitors ... The molecule contains numerous sulfhydryl groups and is glycosylated. This type of enzyme is found widespread in many ...https://www.biosynth.com/en/technology/aquaspark-chemiluminescence/aquaspark-tm.html
Anti-inf lammatory and Antioxidant Effects of Captopri l Compared to Methy lpredniso lone in l-Arginine-Induced Acute...
Measurement of pancreatic lipase activity in serum by a kinetic colorimetric assay using a new chromogenic substrate. Ann Clin ... Mikrut K, Kupsz J, Koźlik J, Krauss H, Pruszyńska-Oszmałek E, Gibas-Dorna M. Angiotensin-converting enzyme inhibitors reduce ... Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82:70-77.CrossRefPubMedGoogle Scholar ... Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-275.PubMedGoogle Scholar ...https://rd.springer.com/article/10.1007/s10620-018-5036-1
Patent US5162504 - Monoclonal antibodies to a new antigenic marker in epithelial prostatic ... - Google Patents
Inhibitors of NAALADase enzyme activity. US5824662 *. Sep 27, 1996. Oct 20, 1998. Guilford Pharmaceuticals Inc.. Treatment of ... 6 5. ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA). The enzyme linked immunosorbent assay (ELISA) was used for general enzyme ... the peroxidase activity was revealed using diaminobenzidine 0.5 mg/ml): H2 O2 (0.01%) substrate in 0.1M Tris buffer pH 7.2 The ... all other reagents used were as described above for cell surface enzyme immunoassay. ...http://www.google.com/patents/US5162504?dq=5,966,702
A novel bound form of plant invertase in rice suspension cells
... and thiol reagents inhibited the enzyme activity, but activity could be restored with DTT. It is suggested that sulfhydryl- ... Invertase Assay. In a total volume of 0.36 mL, the assay mixture contained 60 L of enzyme solution, 0.05 M sodium phosphate, pH ... Effect of Reaction Products on Invertase Activities. Fructose and glucose were inhibitors (Figure 8). Five mM of fructose or ... The purified enzyme responded to sucrose concentration according to Michaelis-Menten kinetics up to 0.1 M, but showed substrate ...http://ejournal.sinica.edu.tw/bbas/content/2002/2/bot432-04.html
Patent US4906565 - Method for the selective detection of microbial nucleotides - Google Patents
... followed by the rapid and specific inhibition of the inactivating enzyme and the release and detection of microbial nucleotides ... in an appropriate assay, such as a bioluminescent assay. ... The ATP-inactivating activity of the enzyme hexokinase, and its ... A preferred group of inhibitors are reagents, particularly those that are specific for the sulfhydryl groups of enzymes. Some ... such as firefly luciferase enzyme and luciferin substrate, in the presence of magnesium and oxygen, to produce photons. These ...http://www.google.com.au/patents/US4906565
Patent US5200313 - Nucleic acid hybridization assay employing detectable anti-hybrid antibodies - Google Patents
Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, ... A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or ... or a participant in an enzyme reaction (e.g., see above list). For example, one can use an enzyme which acts upon a substrate ... Fractions are assayed for β-galactosidase activity and antibody binding activity. Fractions having both activities are pooled. ...http://www.google.ca/patents/US5200313
Patent US6344486 - Benzamide and sulfonamide substituted aminoguanidines and alkoxyguanidines ... - Google Patents
... or are intermediates useful for forming compounds having antithrombotic activity. The invention includes a composition for ... Also described are methods for preparing the compounds of Formula I. Certain of the compounds exhibit antithrombotic activity ... Barrett, A.J., "Proteinase inhibitors: potential drugs?" in Enzyme Inhibitors as Drugs, Sandler, M., ed., The MacMillan Press ... Substrate solutions were prepared at a concentration of 51 mM (51,,Km=1.3 mM) in assay buffer. Final DMSO concentration was 4.3 ...http://www.google.com/patents/US6344486?dq=7493558
Complex chemical libraries - Patent # 6800444 - PatentGenius
In addition, compounds of the present invention are useful as research reagents. ... while low levels of activity are observed on monomeric substrate. As a result, competitive inhibitors of PLA.sub.2 either have ... enzyme binding assays, and selection assays. Another method involves fixing one letter at a time. A furthermethod involves the ... Reagents are kept on ice during the course of the experiment, and the remaining portions are stored at -20.degree. C.. Library ...http://www.patentgenius.com/patent/6800444.html
Molecules | Free Full-Text | Modification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching | HTML
This enzyme, SsoPox, which is a lactonase and phosphotriesterase, provides a means of interrupting bacterial communication ... This work sets out to provide a self-assembled biopolymer capsule activated with a multi-functional enzyme for localized ... Determining Lactonase Activity of Modified SsoPox. The modified SsoPox enzyme activity was verified using two reporter cells ... Verification of SsoPox binding to chitosan films and capsules was completed using enzyme labeled with DyLightTM Sulfhydryl- ...https://www.mdpi.com/1420-3049/23/2/341/htm
... thereby inhibiting the enzyme activity by obstructing access of substrate to the active site. We also clarified that Y-700 is ... Enzyme Assay. The molybdenum-pterin sites of both XDH and XO are structurally equivalent (Enroth et al., 2000). Thus, steady- ... The reagents were mixed with the enzyme anaerobically and incubated at room temperature for 1 h. Under aerobic conditions, ... Synthesis and structure-activity relationships of 1-phenylpyrazoles as xanthine oxidase inhibitors. Bioorg Med Chem Lett 11: ...http://jpet.aspetjournals.org/content/311/2/519
SMART: Secondary literature for VKc domain
Removal of the fourth substrate, oxygen, resulted in the loss of more than 99% of carboxylase activity. The sulfhydryl reagent ... Using an adapted assay that requires an enzyme aliquot that forms only 5 pmoles vitamin K, we were able to demonstrate vitamin ... K1 ratios it was possible to grade the antagonism of vitamin K epoxide reductase activity by known and suspected inhibitors. ... Dithiothreitol and CHAPS are essential for activity, but high concentrations of glycerol reduces the activity. The enzyme is ...http://smart.embl.de/smart/show_secondary.cgi?domain=VKc
A 1,536-Well-Based Kinetic HTS Assay for Inhibitors of Schistosoma mansoni Thioredoxin Glutathione Reductase
Three microliters of reagents (100. μM NADPH in row 32 as no-enzyme control and 100. μM NADPH/15 nM TGR, in rows 1-31) were ... μl in black clear-bottom assay plates). The assay plate was incubated for 15. min at room temperature, and then substrate was ... The assay protocol is detailed in Table 1. The enzymatic activity associated with each well was computed from the change in ... Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82:70-77. [PubMed]. 19. Riddles PW. Blakeley RL. Zerner B. Ellman's ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2669305/
Glutathiolation Enhances the Degradation of γC-crystallin in Lens and Reticulocyte Lysates, Partially via the Ubiquitin...
The activities of the ubiquitin-activating enzyme (E1) and the ubiquitin-conjugating enzyme (E2) were reduced when the GSSG-GSH ... Protein concentrations were determined by using a protein assay reagent (Coomassie Plus; Pierce, Rockford, IL), with bovine ... Consistent with previous studies that have shown that native γ-crystallin is a poor substrate for the proteasome, 32 43 44 ... The state of sulfhydryl groups in normal and cataractous human lens proteins. I. Nuclear region. Exp Eye Res. 1978;26:407-417. ...http://iovs.arvojournals.org/article.aspx?articleid=2126033
Proline residues link the active site to transmembrane domain movements in human nucleoside triphosphate diphosphohydrolase 3 ...
... modulating enzyme activity, and substrate specificity. Using site-directed mutagenesis, the roles of the conserved proline ... Protein assay. Protein concentrations were determined using the Bio-Rad protein assay reagent with the modifications of ... Cysteine residues (V42C and G489C) were introduced in the "free sulfhydryl-less" C10S/C501S/C509S NTPDase3 background (C10S/ ... Due to the scarcity of specific inhibitors and genetically modified animals, the functions of most individual NTPDases are ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2947659/
US6617125B2 - Compositions for enhanced catalyzed reporter deposition - Google Patents
A kit is provided containing an enhancing reagent for enhancing the detection of an enzyme reaction as described herein, ... comprising a detectably labeled phenol-containing molecule with a peroxidase enzyme in the presence of an enhancing reagent, ... the enhancing reagent comprising an organic compound having the structure wherein each R is independently selected from the ... A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting ...https://patents.google.com/patent/US6617125B2/en
Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer
Recent developments in microarray-based enzyme assays: from functional annotation to substrate/inhibitor fingerprinting. Anal ... Luminescent quantum dots fluorescence resonance energy transfer-based probes for enzymatic activity and enzyme inhibitors. Anal ... low consumption of reagents and samples, and decrease in errors between inter-sampling. Although many of powerful analytical ... for collagenase were conjugated on a single QD through a sulfhydryl group of cysteine residue. When the probes were first ...http://thno.org/v02p0127.htm
Protocols and Video Articles Authored by Taisuke Tomita
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag' ... Here we show that the activity of β-site APP cleaving enzyme-1 (BACE1), the rate-limiting enzyme for amyloid-β peptide (Aβ) ... opening the way to design of substrate-specific γ-secretase inhibitors that would reduce the amyloid burden without significant ... which enables identification of the hydrophilic environment by the accessibility of sulfhydryl reagents to cysteine residues ...https://www.jove.com/author/Taisuke_Tomita
US5719041A - DNA encoding ecotin homologs - Google Patents
General Activity Assays. Routine enzyme inhibition assays were conducted in a microtiter format and absorbance changes were ... Controls lacking inhibitor and/or enzyme were also assayed to assess the uninhibited rates and the background substrate ... TFPI inhibitors and methods of use WO2010071894A3 (en) * 2008-12-19. 2010-10-07. Baxter International Inc.. Tfpi inhibitors and ... The sample (plasma and inhibitor) and reagent (CaCl2 /TF) were automatically mixed together after a 2 min incubation at 37° C. ...https://patents.google.com/patent/US5719041?oq=patent%3A5992892
James Swartz | Stanford Medicine Profiles
Protein purification was avoided because the expressed enzyme activity was significantly greater than the background activity ... These results are important for cell-free technology and also suggest an assay for intrinsic in vivo protein synthesis activity ... and identify a fluorogenic substrate that links activity after oxygen exposure to the generation of a fluorescent signal. We ... Prolonging cell-free protein synthesis by selective reagent additions BIOTECHNOLOGY PROGRESS Kim, D. M., Swartz, J. R. 2000; 16 ...https://med.stanford.edu/profiles/james-swartz
Cysteine Protease & Regulator Proteins - Creative Biomart
The first step of the peptide hydrolysis mechanism involves the deprotonation of the sulfhydryl group in the enzyme's active ... Cysteine proteases have a cysteine residue in their active side and therefore employ the sulfhydryl group of the side chain of ... Cathepsin B is a lysosomal cysteine protease with both endopeptidase and dipeptidyl carboxypeptidase activities. The substrate ... Cruzain is an excellent target for the development of irreversible inhibitors due to the presence of the enzyme in all stages ...https://www.creativebiomart.net/researcharea-cysteine-protease-regulator-proteins_958.htm
US7229810B2 - Polymer conjugates of proteinases - Google Patents
... were not observed when assays of proteolytic activity were performed with a substrate of low molecular weight (see He, Z., et ... of graphs of residual enzymatic activity versus time of preincubation of the enzyme preparation in the absence of substrate. As ... preferably using one or more art-known inhibitors of proteinase activity that will be familiar to the ordinarily skilled ... The methods of this invention are similarly applicable to other types of proteinases and to other conjugation reagents. ...https://patents.google.com/patent/US7229810?oq=6437692