Toxins | Free Full-Text | Type II Toxin-Antitoxin Systems in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803 | HTML
... suggesting extensive potential for toxicity-related RNA degradation and toxin-mediated transcriptome remodeling. Of particular ... asRNA, antisense RNA; PIN, PIN domain (homologues of the "pilT N"-terminal domain); Hic, Hic domain (hif-contiguous); YcfA, ... Stazic, D.; Lindell, D.; Steglich, C. Antisense RNA protects mRNA from RNase E degradation by RNA-RNA duplex formation during ... asRNA, antisense RNA; PIN, PIN domain (homologues of the "pilT N"-terminal domain); Hic, Hic domain (hif-contiguous); YcfA, ...http://www.mdpi.com/2072-6651/8/7/228/htm
IJMS | Free Full-Text | Noncoding RNA in Oncogenesis: A New Era of Identifying Key Players | HTML
At the end, we will also discuss recently unraveled activities of circular RNAs (circRNAs). ... New discoveries and accelerating progresses in the field of noncoding RNAs (ncRNAs) continuously challenges our deep-rooted ... PTENP1 is the PTEN pseudogene and encodes two antisense RNA (asRNA) transcripts, asRNA α and β . The α asRNA isoform can ... The β asRNA isoform interacts with the PTENP1 lncRNA through RNA-RNA pairing, which can maintain stable PTENP1 lncRNA levels in ...http://mdpi.com/1422-0067/14/9/18319/htm
Production of Anthocyanins in Grape Cell Cultures: A Potential Source of Raw Material for Pharmaceutical, Food, and Cosmetic...
... pairing of the normal sense RNAs and antisense RNAs (asRNA) arising from aberrant transcription of the transgene; 3) pairing of ... pairing of the normal sense RNAs and antisense RNAs (asRNA) arising from aberrant transcription of the transgene; 3) pairing of ... pairing of transcripts with antisense RNA (asRNA) produced by RNAdirected RNA polymerase (RdRP) [138, 139]. ... pairing of transcripts with antisense RNA (asRNA) produced by RNAdirected RNA polymerase (RdRP) [138, 139]. ...https://www.intechopen.com/books/the-mediterranean-genetic-code-grapevine-and-olive/production-of-anthocyanins-in-grape-cell-cultures-a-potential-source-of-raw-material-for-pharmaceuti/
CH391L/S13/CleanGenomes - OpenWetWare
mRNA Disruption using Antisense RNA. Antisense RNA (asRNA) is a single strand of RNA that complementary to an mRNA inside a ... When antisense RNA base pairs with mRNA, the mRNA is unable to be translated. Cells that can't survive in the presence of ... Antisense RNA disruption can only work is there is an adequate amount of mRNA to disrupt. Intracellular signaling polypeptides ... Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science. 2001 Sep 21; ...https://openwetware.org/wiki/?title=CH391L/S13/CleanGenomes&oldid=672758
Variance explained by whole genome sequence variants in coding and regulatory genome annotations for six dairy traits | BMC...
23]. Long noncoding RNA (lncRNA) and antisense RNA (asRNA) were obtained from the study by Koufariotis L et al. . ... Annotation of long noncoding RNA and antisense RNA. Long noncoding RNA were obtained from the study by Koufariotis et al.  ... long noncoding RNA (lncRNA), antisense RNA (asRNA) and untranslated regions (UTR). Out of these 20 annotation classes, 13 were ... Long noncoding RNA in genome regulation: prospects and mechanisms. RNA Biol. 2010;7(5):582-5.View ArticlePubMedPubMed Central ...https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4617-x
Andes Biotechnologies Global Jobs - CB Insights
The company has discovered two novel families of long noncoding RNAs: sense (S-RNA) and antisense noncoding (AS-RNA). Both are ... The company has discovered two novel families of long noncoding RNAs: sense (S-RNA) and antisense noncoding (AS-RNA). Both are ... developed by Andes Biotechnologies is based on oligonucleotide therapeutics that target the novel long noncoding regulatory RNA ...https://www.cbinsights.com/company/andes-biotechnologies-global-jobs
There is a world beyond protein mutations: the role of non-coding RNAs in melanomagenesis - Swoboda - 2013 - Experimental...
... long intergenic non-coding RNAs (lincRNA), which are located away from protein-coding regions, antisense RNAs (asRNA), which ... It is known that asRNA levels influence sense RNA levels. Epigenetic changes are another way to vary RNA expression. The ... which contain not only non-coding RNAs but also coding RNAs although to a lesser extent (e.g. FGF-2 and its asRNA FGF-AS [69 ... Table 1. Conceivable applications based on non-coding RNAs. Diagnosis. Presence of specific non-coding RNAs as biomarker for ...http://onlinelibrary.wiley.com/doi/10.1111/exd.12117/full
Iron Deprivation in Synechocystis: Inference of Pathways, Non-coding RNAs, and Regulatory Elements from Comprehensive...
... where one of the three Fur homologs is controlled by a cis-acting antisense RNA (asRNA) (Hernandez et al. 2006). Similar asRNAs ... 2011 Chloroplast RNase J compensates for inefficient transcription termination by removal of antisense RNA. RNA 17(12): 2165- ... Statistical and clustering analyses identified 10 small RNAs, 62 antisense RNAs, four 5′UTRs, and seven intragenic elements as ... the asRNA to isiA; (ii) sll0027-as1, the asRNA to ndhD4 encoding the NDH subunit D4; (iii) sll1867-as1, the asRNA to psbA3; (iv ...http://www.g3journal.org/content/2/12/1475
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
In this study, fine-tuning of the fatty acid pathway in Escherichia coli with antisense RNA (asRNA) to balance the demands on ... Fine-tuning of the fatty acid pathway by synthetic antisense RNA for enhanced (2S)-naringenin production from L-tyrosine in ... It was demonstrated that the gene-silencing effect of asRNA could be tuned by directing asRNA to different positions in the 5'- ... To establish an efficient asRNA system, the relationship between sequence and function for asRNA was explored. ...https://www.jove.com/visualize?author=Jian+Chen
SMART: Secondary literature for RPOL9 domain
... rot RNA as a natural antisense RNA (asRNA) regulator of l(2)tid expression, expressed in the same temporal and spatial manner ... Human RNA polymerase II subunit hRPB14 is homologous to yeast RNA polymerase I, II, and III subunits (AC19 and RPB11) and is ... l(2)tid knock-out by antisense RNA yielded late embryonic lethality resulting from multiple morphogenetic defects. ... Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III. ...http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=RPOL9
Frontiers | Emerging Putative Associations between Non-Coding RNAs and Protein-Coding Genes in Neuropathic Pain: Added Value...
... antisense RNA (31 asRNA in SN, and 12 in DRG) and pseudogenes (456 in SN, 56 in DRG). In particular, contextual analysis ... little is currently known about the role of non-coding RNAs (ncRNAs). This leaves open questions about the potential effects of ... with neighborhood defined by a fixed genomic distance from long or antisense ncRNA loci, and of parent genes associated with ... with neighborhood defined by a fixed genomic distance from long or antisense ncRNA loci, and of parent genes associated with ...https://www.frontiersin.org/articles/10.3389/fneur.2016.00168/full
Directional RNA-seq reveals highly complex condition-dependent transcriptomes in E. coli K12 through accurate full-length...
... and a large portion of genes and intergenic regions have antisense RNA (asRNA) and non-coding RNA (ncRNA) transcripts, ... RNA-seqProkaryote E. coli TranscriptomeAssemblyTranscription start siteAlternative operonAntisense RNANon-coding RNA ... generating non-coding RNAs (ncRNAs) [1, 2] and anti-sense RNAs (asRNAs) [3, 4], respectively. Accumulating body of evidence ... 12864_2013_5230_MOESM12_ESM.xlsx Additional file 12:The predicted antisense RNAs in the E. coli samples.(XLSX 3 MB) ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-520
Antisense RNA - Wikipedia
Antisense RNA (asRNA), also referred as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, ... In the presence of the asRNA RNA I, RNA I and RNA II forms a duplex which introduces a conformational change of RNA II. ... RNA-RNA interactions either in nucleus or cytoplasm and RNA-protein interactions (epigenetic). Antisense RNAs can be ... The asRNA that is responsible for this silencing effect is antisense non-coding RNA in the INK locus (ANRIL), which is ...https://en.wikipedia.org/wiki/Antisense_RNA
Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus | Genome Biology | Full Text
... ncRNA and antisense RNA (asRNA) coding genes).. Genome-wide RNA decay. The median half-life and the median decay rate of ... Distribution of RNA decay rates and RNA half lives using the two phase decay step or the twofold decay step method. (a) RNA ... Rate of RNA polymerase transcription. The fast RNA turnover we found for Prochlorococcus made us wonder whether both RNA ... RNA isolation. Total RNA was extracted from cells on filters using a hot-phenol method described previously [24, 50]. Total ...https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-5-r54
Chromatin: From Structure to Epigenetics
Antisense RNAs. Jane Mellor, University of Oxford. Dr. Mellor discussed antisense transcripts in yeast (asRNA). These RNAs bind ... They determined that these asRNA "reset" the state of the promoter in many cells and high levels of asRNA keep the genes ... They showed that the RNA-protein interaction of JARID2 enhances its binding to Ezh2. They proposed a mechanism by which JAIRD2 ... Mellor noted that genes with a high incidence of asRNA tend to have non-canonical distributions of H3K4me3 levels. Her lab ...https://epigenie.com/conferences/chromatin-from-structure-to-epigenetics/
US20080108513A1 - Cellular Arrays - Google Patents
Included within this definition are antisense RNA ("asRNA") molecules involved in gene regulation by bacteria. Antisense RNA ... As used herein, the term "antisense" is used in reference to RNA sequences that are complementary to a specific RNA sequence (e ... of RNA by electrophoresis of RNA on agarose gels to fractionate the RNA according to size followed by transfer of the RNA from ... which is transcribed into an anti-sense RNA sequence, a ribozyme or double-stranded RNA, etc.), or a regulatory sequence (e.g ...https://patents.google.com/patent/US20080108513A1/en
Comparative analysis of non-coding RNAs in the antibiotic-producing Streptomyces bacteria | BMC Genomics | Full Text
Expression profiles of antisense RNAs within secondary metabolite clusters. (A) Expression profile of the asRNA expressed ... we discovered hundreds of novel cis-antisense RNAs and intergenic small RNAs (sRNAs). We identified a ubiquitous antisense RNA ... Antisense RNAs resulted from the divergent transcription of these two genes  and an independent antisense RNA was expressed ... Streptomyces Non-coding RNAsRNAAntisense RNASecondary metabolic gene clusterAntibioticRNA degradation ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-558
Plasmodium falciparum UvrD activities are downregulated by DNA-interacting compounds and its dsRNA inhibits malaria parasite...
These purified templates were used for in vitro transcription to generate sense RNA (sRNA) and anti-sense RNA (asRNA) using T7 ... Double stranded (ds) RNA preparation. The clone of PfUDN in the pGEMT easy vector of Promega (Madison, WI, USA) was used as a ... shows ds RNA preparation of green fluorescent protein (GFP) (lane 1) and PfUDN (lane 2) lane M is the marker. B. shows the ... For the production of dsRNA, equal amounts of sRNA and asRNA were mixed and incubated at 65°C for 30 min, and then the ...https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-15-9
RNA associated with the nerve healing process identified - African Biosciences Blog
... "anti-sense" RNA (AS-RNA) is expressed when nerves are injured. Their experiments in mice show that the AS-RNA helps to regulate ... Inducing expression of anti-sense RNA (right) inhibits the production of myelin (red) in mouse nerve cells compared to ... Later, when new nerve growth has occurred, the AS-RNA expression dies down to allow the re-myelination to occur.. In further ... Types of RNA: mRNA, rRNA and tRNA. RNA or ribonucleic acid is a polymer of nucleotides which is made up of a ribose sugar, a ...http://blog.africanbio.com/2017/08/rna-associated-with-nerve-healing.html
CoreGenomics: RNA-seq contextualised: what's possible in 2D and in situ RNA sequencing
They were able to demonstrate that nuclear RNA was twice as likely to be non-coding, and antisense mRNA was almost twice as ... FISSEQ generated 43% mRNA reads, 43% rRNA, 7% ncRNA, and 7% asRNA. ... RNA-seq shows the differential gene expression in bulk cells from a tissue, RNA-FISH confirms if this DGE is located in ... In their discussion they propose that the poor correlation of FISEQ to RNA-seq for genes involved in RNA and protein processing ...http://core-genomics.blogspot.com/2015/06/rna-seq-contextualised-whats-possibile.html
AsponA antisense RNA - Wikipedia
AsponA is a small asRNA transcribed antisense to the penicillin-binding protein 1A gene called ponA. It was identified by ... Gómez-Lozano, María; Marvig, Rasmus L.; Tulstrup, Monica V. L.; Molin, Søren (2014-09-11). "Expression of antisense small RNAs ... profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs". PLOS One ...https://en.wikipedia.org/wiki/AsponA_antisense_RNA
RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE - Patent application
... the anti-sense preparation. However, when Rr-luc mRNA was included in the reaction with the 32P-radiolabeled, capped Rr-asRNA, ... isolated RNA (partially purified RNA, essentially pure RNA, synthetic RNA, recombinantly produced RNA), as well as altered RNA ... As used herein, the terms RNA, RNA molecule(s), RNA segment(s) and RNA fragment(s) are used interchangeably to refer to RNA ... All of these are referred to as 21-23 nt RNAs that mediate RNA interference. As used herein, the term isolated RNA includes RNA ...http://www.patentsencyclopedia.com/app/20160032288
Biopolymers and Cell
Antisense RNAs. Molekulyarnaya Onkologiya. 1987; 21(1):28-32.  Wickstrom EL, Bacon TA, Gonzalez A, Freeman DL, Lyman GH, ... An expression of specific RNA in cell cultures with an integrated asRNA genome and their possible correlation with the ... 6] Izant JG, Weintraub H. Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic ... An investigation of the effect of antisense RNA gene on bovine leukaemia virus reproduction in cell culture. Vet Microbiol. ...http://biopolymers.org.ua/content/9/4/059/
Eric E. Swayze, Encinitas US - Patent applications
ANTISENSE COMPOUNDS - Provided herein are gapmer oligomeric compounds for reduction of target RNA in vivo comprising different ... The modified oligomeric compounds include siRNA and asRNA having at least one affinity modification.. 12-23-2010. ... Certain such antisense compounds are useful as RNase H antisense compounds, as RNAi compounds, and/or as modulators of splicing ... Certain such antisense compounds are useful as RNase H antisense compounds or as RNAi compounds.. 12-22-2011. ...http://www.patentsencyclopedia.com/inventor/eric-e-swayze-encinitas-us-1/