*  Toxins | Free Full-Text | Type II Toxin-Antitoxin Systems in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803 | HTML
... suggesting extensive potential for toxicity-related RNA degradation and toxin-mediated transcriptome remodeling. Of particular ... asRNA, antisense RNA; PIN, PIN domain (homologues of the "pilT N"-terminal domain); Hic, Hic domain (hif-contiguous); YcfA, ... Stazic, D.; Lindell, D.; Steglich, C. Antisense RNA protects mRNA from RNase E degradation by RNA-RNA duplex formation during ... asRNA, antisense RNA; PIN, PIN domain (homologues of the "pilT N"-terminal domain); Hic, Hic domain (hif-contiguous); YcfA, ...
  http://www.mdpi.com/2072-6651/8/7/228/htm
*  IJMS | Free Full-Text | Noncoding RNA in Oncogenesis: A New Era of Identifying Key Players | HTML
At the end, we will also discuss recently unraveled activities of circular RNAs (circRNAs). ... New discoveries and accelerating progresses in the field of noncoding RNAs (ncRNAs) continuously challenges our deep-rooted ... PTENP1 is the PTEN pseudogene and encodes two antisense RNA (asRNA) transcripts, asRNA α and β [161]. The α asRNA isoform can ... The β asRNA isoform interacts with the PTENP1 lncRNA through RNA-RNA pairing, which can maintain stable PTENP1 lncRNA levels in ...
  http://mdpi.com/1422-0067/14/9/18319/htm
*  Production of Anthocyanins in Grape Cell Cultures: A Potential Source of Raw Material for Pharmaceutical, Food, and Cosmetic...
... pairing of the normal sense RNAs and antisense RNAs (asRNA) arising from aberrant transcription of the transgene; 3) pairing of ... pairing of the normal sense RNAs and antisense RNAs (asRNA) arising from aberrant transcription of the transgene; 3) pairing of ... pairing of transcripts with antisense RNA (asRNA) produced by RNAdirected RNA polymerase (RdRP) [138, 139]. ... pairing of transcripts with antisense RNA (asRNA) produced by RNAdirected RNA polymerase (RdRP) [138, 139]. ...
  https://www.intechopen.com/books/the-mediterranean-genetic-code-grapevine-and-olive/production-of-anthocyanins-in-grape-cell-cultures-a-potential-source-of-raw-material-for-pharmaceuti/
*  CH391L/S13/CleanGenomes - OpenWetWare
mRNA Disruption using Antisense RNA. Antisense RNA (asRNA) is a single strand of RNA that complementary to an mRNA inside a ... When antisense RNA base pairs with mRNA, the mRNA is unable to be translated. Cells that can't survive in the presence of ... Antisense RNA disruption can only work is there is an adequate amount of mRNA to disrupt. Intracellular signaling polypeptides ... Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science. 2001 Sep 21; ...
  https://openwetware.org/wiki/?title=CH391L/S13/CleanGenomes&oldid=672758
*  Variance explained by whole genome sequence variants in coding and regulatory genome annotations for six dairy traits | BMC...
23]. Long noncoding RNA (lncRNA) and antisense RNA (asRNA) were obtained from the study by Koufariotis L et al. [49]. ... Annotation of long noncoding RNA and antisense RNA. Long noncoding RNA were obtained from the study by Koufariotis et al. [49] ... long noncoding RNA (lncRNA), antisense RNA (asRNA) and untranslated regions (UTR). Out of these 20 annotation classes, 13 were ... Long noncoding RNA in genome regulation: prospects and mechanisms. RNA Biol. 2010;7(5):582-5.View ArticlePubMedPubMed Central ...
  https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4617-x
*  Andes Biotechnologies Global Jobs - CB Insights
The company has discovered two novel families of long noncoding RNAs: sense (S-RNA) and antisense noncoding (AS-RNA). Both are ... The company has discovered two novel families of long noncoding RNAs: sense (S-RNA) and antisense noncoding (AS-RNA). Both are ... developed by Andes Biotechnologies is based on oligonucleotide therapeutics that target the novel long noncoding regulatory RNA ...
  https://www.cbinsights.com/company/andes-biotechnologies-global-jobs
*  There is a world beyond protein mutations: the role of non-coding RNAs in melanomagenesis - Swoboda - 2013 - Experimental...
... long intergenic non-coding RNAs (lincRNA), which are located away from protein-coding regions, antisense RNAs (asRNA), which ... It is known that asRNA levels influence sense RNA levels. Epigenetic changes are another way to vary RNA expression. The ... which contain not only non-coding RNAs but also coding RNAs although to a lesser extent (e.g. FGF-2 and its asRNA FGF-AS [69 ... Table 1. Conceivable applications based on non-coding RNAs. Diagnosis. Presence of specific non-coding RNAs as biomarker for ...
  http://onlinelibrary.wiley.com/doi/10.1111/exd.12117/full
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
In this study, fine-tuning of the fatty acid pathway in Escherichia coli with antisense RNA (asRNA) to balance the demands on ... Fine-tuning of the fatty acid pathway by synthetic antisense RNA for enhanced (2S)-naringenin production from L-tyrosine in ... It was demonstrated that the gene-silencing effect of asRNA could be tuned by directing asRNA to different positions in the 5'- ... To establish an efficient asRNA system, the relationship between sequence and function for asRNA was explored. ...
  https://www.jove.com/visualize?author=Jian+Chen
*  SMART: Secondary literature for RPOL9 domain
... rot RNA as a natural antisense RNA (asRNA) regulator of l(2)tid expression, expressed in the same temporal and spatial manner ... Human RNA polymerase II subunit hRPB14 is homologous to yeast RNA polymerase I, II, and III subunits (AC19 and RPB11) and is ... l(2)tid knock-out by antisense RNA yielded late embryonic lethality resulting from multiple morphogenetic defects. ... Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III. ...
  http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=RPOL9
*  Frontiers | Emerging Putative Associations between Non-Coding RNAs and Protein-Coding Genes in Neuropathic Pain: Added Value...
... antisense RNA (31 asRNA in SN, and 12 in DRG) and pseudogenes (456 in SN, 56 in DRG). In particular, contextual analysis ... little is currently known about the role of non-coding RNAs (ncRNAs). This leaves open questions about the potential effects of ... with neighborhood defined by a fixed genomic distance from long or antisense ncRNA loci, and of parent genes associated with ... with neighborhood defined by a fixed genomic distance from long or antisense ncRNA loci, and of parent genes associated with ...
  https://www.frontiersin.org/articles/10.3389/fneur.2016.00168/full
*  Directional RNA-seq reveals highly complex condition-dependent transcriptomes in E. coli K12 through accurate full-length...
... and a large portion of genes and intergenic regions have antisense RNA (asRNA) and non-coding RNA (ncRNA) transcripts, ... RNA-seqProkaryote E. coli TranscriptomeAssemblyTranscription start siteAlternative operonAntisense RNANon-coding RNA ... generating non-coding RNAs (ncRNAs) [1, 2] and anti-sense RNAs (asRNAs) [3, 4], respectively. Accumulating body of evidence ... 12864_2013_5230_MOESM12_ESM.xlsx Additional file 12:The predicted antisense RNAs in the E. coli samples.(XLSX 3 MB) ...
  https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-520
*  Antisense RNA - Wikipedia
Antisense RNA (asRNA), also referred as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, ... In the presence of the asRNA RNA I, RNA I and RNA II forms a duplex which introduces a conformational change of RNA II. ... RNA-RNA interactions either in nucleus or cytoplasm and RNA-protein interactions (epigenetic). Antisense RNAs can be ... The asRNA that is responsible for this silencing effect is antisense non-coding RNA in the INK locus (ANRIL), which is ...
  https://en.wikipedia.org/wiki/Antisense_RNA
*  Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus | Genome Biology | Full Text
... ncRNA and antisense RNA (asRNA) coding genes).. Genome-wide RNA decay. The median half-life and the median decay rate of ... Distribution of RNA decay rates and RNA half lives using the two phase decay step or the twofold decay step method. (a) RNA ... Rate of RNA polymerase transcription. The fast RNA turnover we found for Prochlorococcus made us wonder whether both RNA ... RNA isolation. Total RNA was extracted from cells on filters using a hot-phenol method described previously [24, 50]. Total ...
  https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-5-r54
*  Comparative analysis of non-coding RNAs in the antibiotic-producing Streptomyces bacteria | BMC Genomics | Full Text
Expression profiles of antisense RNAs within secondary metabolite clusters. (A) Expression profile of the asRNA expressed ... we discovered hundreds of novel cis-antisense RNAs and intergenic small RNAs (sRNAs). We identified a ubiquitous antisense RNA ... Antisense RNAs resulted from the divergent transcription of these two genes [60] and an independent antisense RNA was expressed ... Streptomyces Non-coding RNAsRNAAntisense RNASecondary metabolic gene clusterAntibioticRNA degradation ...
  https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-558
*  Plasmodium falciparum UvrD activities are downregulated by DNA-interacting compounds and its dsRNA inhibits malaria parasite...
These purified templates were used for in vitro transcription to generate sense RNA (sRNA) and anti-sense RNA (asRNA) using T7 ... Double stranded (ds) RNA preparation. The clone of PfUDN in the pGEMT easy vector of Promega (Madison, WI, USA) was used as a ... shows ds RNA preparation of green fluorescent protein (GFP) (lane 1) and PfUDN (lane 2) lane M is the marker. B. shows the ... For the production of dsRNA, equal amounts of sRNA and asRNA were mixed and incubated at 65°C for 30 min, and then the ...
  https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-15-9
*  AsponA antisense RNA - Wikipedia
AsponA is a small asRNA transcribed antisense to the penicillin-binding protein 1A gene called ponA. It was identified by ... Gómez-Lozano, María; Marvig, Rasmus L.; Tulstrup, Monica V. L.; Molin, Søren (2014-09-11). "Expression of antisense small RNAs ... profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs". PLOS One ...
  https://en.wikipedia.org/wiki/AsponA_antisense_RNA
*  RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE - Patent application
... the anti-sense preparation. However, when Rr-luc mRNA was included in the reaction with the 32P-radiolabeled, capped Rr-asRNA, ... isolated RNA (partially purified RNA, essentially pure RNA, synthetic RNA, recombinantly produced RNA), as well as altered RNA ... As used herein, the terms RNA, RNA molecule(s), RNA segment(s) and RNA fragment(s) are used interchangeably to refer to RNA ... All of these are referred to as 21-23 nt RNAs that mediate RNA interference. As used herein, the term isolated RNA includes RNA ...
  http://www.patentsencyclopedia.com/app/20160032288
*  Biopolymers and Cell
Antisense RNAs. Molekulyarnaya Onkologiya. 1987; 21(1):28-32. [2] Wickstrom EL, Bacon TA, Gonzalez A, Freeman DL, Lyman GH, ... An expression of specific RNA in cell cultures with an integrated asRNA genome and their possible correlation with the ... 6] Izant JG, Weintraub H. Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic ... An investigation of the effect of antisense RNA gene on bovine leukaemia virus reproduction in cell culture. Vet Microbiol. ...
  http://biopolymers.org.ua/content/9/4/059/
*  Eric E. Swayze, Encinitas US - Patent applications
ANTISENSE COMPOUNDS - Provided herein are gapmer oligomeric compounds for reduction of target RNA in vivo comprising different ... The modified oligomeric compounds include siRNA and asRNA having at least one affinity modification.. 12-23-2010. ... Certain such antisense compounds are useful as RNase H antisense compounds, as RNAi compounds, and/or as modulators of splicing ... Certain such antisense compounds are useful as RNase H antisense compounds or as RNAi compounds.. 12-22-2011. ...
  http://www.patentsencyclopedia.com/inventor/eric-e-swayze-encinitas-us-1/
*  Most recent papers with the keyword porf2 | Read by QxMD
... www.readbyqxmd.com/read/26104584/recombinant-hepatitis-e-virus-like-particles-can-function-as-rna-nanocarriers ... We found that sense and antisense PolyA of simian virus 40 (SV40PolyA, 240 bp) eliminated the repression of GFP gene expression ... The protein encoded by ORF2 in hepatitis E virus (HEV) is the only capsid protein for this single-stranded RNA virus. It was ... The hepatitis E virus (HEV), a nonenveloped RNA virus, is the causative agent of hepatitis E. The mode by which HEV attaches to ...
  https://www.readbyqxmd.com/keyword/38355
*  LncRNAs: From Basic Research to Medical Application
Phosphatase and tensin homolog pseudogene 1 antisense RNA (PTENP1-AS) may be an example of the RNA-RNA interaction. Because of ... PTENP1 asRNA. (α,β,unspliced). 1. the α isform suppress the expression of PTEN by localize on the PTEN promoter and interact ... RNA and RNA interaction: rap-rna modification of the RAP method can provide a means to detect RNA-RNA interactions, even to ... Until now, except for messengers RNA (mRNA) and other functional RNAs, such as transfer RNA (tRNA), small nuclear RNA (snRNA), ...
  http://www.ijbs.com/v13p0295.htm
*  Natural antisense RNAs in the nervous system : Sussex Research Online
Natural antisense RNAs are endogenous molecules that are complementary to RNA transcripts of already established function. They were discovered first in prokaryotes in which they are now recognised as an important component of molecular mechanisms involved in the regulation of gene expression. Recently, through the cumulative efforts of molecular biologists and bioinformaticians, natural antisense RNAs have been demonstrated in significant numbers in eukaryotic systems also. Probably the most exciting outcome of these studies is that natural antisense RNAs are particularly prevalent in the nervous system. Here we discuss the major known types of natural antisense RNAs in eukaryotic systems and focus on their potential roles in the regulation of gene expression in the brain.. ...
  http://sro.sussex.ac.uk/30312/
*  Permanent Inhibition of Angiotensinogen Synthesis by Antisense RNA Expression | Hypertension
This study clearly demonstrates that rat AOGEN mRNA and protein can be reduced by the permanent expression of an AS RNA in cell culture. The mechanism of this decrease is speculative at the present. The AS effect could be due to an inhibition of splicing of the pre-mRNA,7 blocking of ribosome binding,12 or modification of the target mRNA in the RNA-RNA duplex by the enzyme unwindase.26 The AS RNA used in the present study could function via all of these mechanisms, since it covers the transcription start site; the exon/intron borders of exon 1, intron 1, and exon 2; and the translation start site. In addition, it overlaps the coding sequence for angiotensin II, the effector hormone of the RAS. To increase the expression, an intron was introduced into the plasmids coding for ...
  http://hyper.ahajournals.org/content/27/3/508
*  Treatement Of Vascular Endothelial Growth Factor (vegf) Related Diseases By Inhibition Of Natural Antisense Transcript To Vegf ...
Oligonucleotide compounds modulate expression and/or function of Vascular Endothelial Growth Factor (VEGF) polynucleotides and encoded products thereof. Methods for treating diseases associated with Vascular Endothelial Growth Factor (VEGF) comprise administering one or more Oligonucleotide compounds designed to inhibit the VEGF natural antisense transcript to patients.
  http://www.freshpatents.com/-dt20150521ptan20150141494.php
*  Staff Profile - Institute for Cell and Molecular Biosciences - Newcastle University
Natural antisense transcripts (NATs). Large proportions of the human genome do not code for proteins and were long considered as junk or 'the dark matter of the genome'. Recent studies, however, have shown that much of the noncoding regions of the genome are actively transcribed. The resulting noncoding RNAs have emerged as essential and abundant regulators of eukaryotic gene expression. Interestingly, noncoding RNAs are implicated in cancer, Alzheimer's and thalassemia. Our research mainly focuses on a particular family of noncoding RNAs, so-called natural antisense transcripts (NATs). NATs are long regulatory RNAs that are transcribed in the opposite direction of protein-coding transcripts and potentially regulate their expression level. Moreover, NATs are hypothesised to enable the evolution of complex organisms.. Wight M, Werner A. The ...
  http://www.ncl.ac.uk/camb/staff/profile/andreaswerner.html
*  An antisense RNA inhibits translation by competing with standby ribosomes
Most antisense RNAs in bacteria inhibit translation by competing with ribosomes for translation initiation regions (TIRs) on nascent mRNA. We propose a mechanism by which an antisense RNA inhibits translation without binding directly to a TIR. The tisAB locus encodes an SOS-induced toxin, and IstR-1 is the antisense RNA that counteracts toxicity. We show that full-length tisAB mRNA (+1) is translationally inactive and endonucleolytic processing produces an active mRNA (+42). IstR-1 binding inhibits translation of this mRNA, and subsequent RNase III cleavage generates a truncated, inactive mRNA (+106). In vitro translation, toeprinting, and structure mapping ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:40737
*  r-nastiseq - Bioconda documentation
Pairs of RNA molecules transcribed from partially or entirely complementary loci are called cis-natural antisense transcripts (cis-NATs), and they play key roles in the regulation of gene expression in many organisms. A promising experimental tool for profiling sense and antisense transcription is strand-specific RNA sequencing (ssRNA-seq). To identify cis-NATs using ssRNA-seq, we developed a new computational method based on model comparison that incorporates the inherent variable efficiency of generating perfectly strand-specific libraries. Applying the method to new ssRNA-seq data from whole root and cell-type specific Arabidopsis libraries confirmed most of the known cis-NAT pairs and identified hundreds of additional cis-NAT pairs.. ...
  http://bioconda.github.io/recipes/r-nastiseq/README.html
*  ALZFORUM | NETWORKING FOR A CURE
In this elegant study, the Ranum group provides novel insight into the presence of antisense and sense transcripts of the C9ORF72 repeat expansion, and RAN translation thereof. Through the use of antibodies toward the repeat motifs of RAN proteins, as well as antibodies against their corresponding unique C-terminal regions, the group confirmed that six C9ORF72 RAN proteins (GPS, GRS, GAS, PAAS, PRAS, GPAS) form aggregates in c9ALS brain, with GPS/AS inclusions also being present in spinal cord motor neurons. Of interest, their studies reveal that the majority of GP-repeat inclusions derive from the antisense transcript, raising the question as to whether sense and antisense transcripts are differentially prone to foci formation or RAN translation. Perhaps most exciting of all, this study provides the first evidence that C9ORF72 RAN proteins are toxic to cells. Future studies examining the toxicity of individual RAN proteins, and the mechanisms by which they ...
  http://www.alzforum.org/papers/ran-proteins-and-rna-foci-antisense-transcripts-c9orf72-als-and-frontotemporal-dementia
*  At3g03300 - Endoribonuclease Dicer homolog 2 - Arabidopsis thaliana (Mouse-ear cress) - At3g03300 gene & protein
Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Involved in the processing of natural small interfering RNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving small dsRNAs into 24 nucleotide nat-siRNAs. Plays an essential role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL4 and RDR6, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. May participate with DCL3 in the production of 24 nucleotide repeat-associated siRNAs ...
  http://www.uniprot.org/uniprot/Q3EBC8
*  BIO-SYNTHESIS - Custom Antibody Custom Peptide Synthesis Custom SiRNA Synthesis: Small Interfering RNA
siRNAs were first discovered by David Baulcombe's group in Norwich, England, as part of post-transcriptional gene silencing (PTGS) in plants, and published their findings in Science in a paper titled "A species of small antisense RNA in posttranscriptional gene silencing in plants". Shortly thereafter, in 2001, synthetic siRNAs were then shown to be able to induce RNAi in mammalian cells by Thomas Tuschl and colleagues in a paper published in Nature. This discovery led to a surge in interest in harnessing RNAi for biomedical research and drug development. ...
  http://bio-synthesis.blogspot.com/2011/03/small-interfering-rna_14.html
*  TREATMENT OF INSULIN RECEPTOR SUBSTRATE 2 (IRS2) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO IRS2 AND...
0241] The Abstract of the disclosure will allow the reader to quickly ascertain the nature of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the following claims. Sequence CWU 1 SEQUENCE LISTING ,160, NUMBER OF SEQ ID NOS: 11 ,210, SEQ ID NO 1 ,211, LENGTH: 7014 ,212, TYPE: DNA ,213, ORGANISM: Homo sapiens ,300, PUBLICATION INFORMATION: ,308, DATABASE ACCESSION NUMBER: NM_003749 ,309, DATABASE ENTRY DATE: 2010-12-25 ,313, RELEVANT RESIDUES IN SEQ ID NO: (1)..(7014) ,400, SEQUENCE: 1 cggggaccgc gacgagcccg ggtcgccgtt ggcagcagca gcagcaacac cagcagcagc 60 agcagccccg gcggcggcgc ggaccccgag cgcccgggcg caccccggct tcccggagcg 120 cgacgcggcg gcagcagccc cggtgcggcc gcgcgcgcct taggctcggc cccgcggctc 180 ggggaccccg actcccggcc cagcgagcgc gtcccccggc gccgcccgag agcccgagga 240 ggcagcggcc gcaggcagcc ggggaggggg gcggccaccg cccgcgccgg gcatcctcag 300 gagccccaga gcgcggaggg cgcggcgccg ccgagcggtg ctggcccccg cgggcctccc 360 cggaccttcc ...
  http://www.patentsencyclopedia.com/app/20120289583
*  TREATMENT OF DYSTROPHIN FAMILY RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO DMD FAMILY - Patent...
0261] The Abstract of the disclosure will allow the reader to quickly ascertain foe nature of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the following claims. Sequence CWU 1 SEQUENCE LISTING ,160, NUMBER OF SEQ ID NOS: 32 ,210, SEQ ID NO 1 ,211, LENGTH: 13993 ,212, TYPE: DNA ,213, ORGANISM: Homo sapiens ,300, PUBLICATION INFORMATION: ,308, DATABASE ACCESSION NUMBER: NM_004006 ,309, DATABASE ENTRY DATE: 2010-08-04 ,313, RELEVANT RESIDUES IN SEQ ID NO: (1)..(13993) ,400, SEQUENCE: 1 tcctggcatc agttactgtg ttgactcact cagtgttggg atcactcact ttccccctac 60 aggactcaga tctgggaggc aattaccttc ggagaaaaac gaataggaaa aactgaagtg 120 ttactttttt taaagctgct gaagtttgtt ggtttctcat tgtttttaag cctactggag 180 caataaagtt tgaagaactt ttaccaggtt ttttttatcg ctgccttgat atacactttt 240 caaaatgctt tggtgggaag aagtagagga ctgttatgaa agagaagatg ttcaaaagaa 300 aacattcaca aaatgggtaa atgcacaatt ttctaagttt gggaagcagc atattgagaa 360 cctcttcagt ...
  http://www.patentsencyclopedia.com/app/20120046345
*  Induction of G alpha i2-specific antisense RNA in vivo inhibits neonatal growth | Science
Guanosine triphosphate-binding regulatory proteins (G proteins) are key elements in transmembrane signaling and have been implicated as regulators of more complex biological processes such as differentiation and development. The G protein G alpha i2 is capable of mediating the inhibitory control of adenylylcyclase and regulates stem cell differentiation to primitive endoderm. Here an antisense RNA to G alpha i2 was expressed in a hybrid RNA construct whose expression was both tissue-specific and induced at birth. Transgenic mice in which the antisense construct was expressed displayed a lack of normal development in targeted organs that correlated with the absence of G alpha i2. The loss of G alpha i2 expression in adipose tissue of the transgenic mice was correlated with a rise in basal levels of adenosine 3',5'-monophosphate (cAMP) and the loss of receptor-mediated inhibition of adenylylcyclase. These data ...
  http://science.sciencemag.org/content/260/5110/991
*  High-resolution transcription atlas of the mitotic cell cycle in budding yeast | Genome Biology | Full Text
We analyzed Gene Ontology (GO) categories of genes overlapped by antisense transcripts. Most of the protein-coding messages opposite the 37 periodic antisense transcripts (13 + 24) fall into GO categories linked with the process of cell division, including cell wall and organelle organization and biosynthesis, regulation of transcription, signal transduction and protein modification, carbohydrate metabolic processes, and cell cycle (Additional file 10). Surprisingly, 15 of the 37 sense transcripts are of unknown function. We carried out a similar analysis for the 43 non-periodic antisense transcripts opposite periodic sense transcripts. As expected, most of these cycling sense messages fall into cell-cycle-related GO categories, including genes involved in bud site selection and polarization (BUD9, GIC1), daughter cell separation from the mother (DSE2, CTS1), cell wall proteins, and so on (Additional file 7). Analysis of GO categories for the remaining 443 ...
  https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-3-r24
*  Secondary structure of HIVaINR antisense RNA[14] predic | Open-i
Secondary structure of HIVaINR antisense RNA[14] predicted by enhanced Mfold [31-33]. This is one of 19 structures predicted, but was chosen to illustrate the e
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2553099_1742-4690-5-79-1&req=4
*  antisense rna
... definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. Look it up now!
  http://www.dictionary.com/browse/antisense-rna
*  Researchers discover novel 'gene toggles' in world's top food crop
Some 240 microRNAs previously had been annotated in rice. Using a high-throughput gene-sequencing technique known as Massively Parallel Signature Sequencing (MPSS), the UD research team analyzed over 4 million small RNAs from 6 rice samples, which yielded 24 new microRNAs, including the unique new group of molecules called natural antisense microRNAs.. When a gene is ready to produce a protein, its two strands of DNA unravel. The first strand, called the "sense" transcript, produces messenger RNA, which carries the recipe for making a specific protein. However, the other strand of DNA may produce a complementary antisense RNA molecule, which sometimes can block production of the protein, thus turning off, or "silencing," the gene.. Blake Meyers, associate professor of plant ...
  http://www.innovations-report.com/html/reports/agricultural-sciences/report-107479.html
*  Apple miRNAs and tasiRNAs with novel regulatory networks | Genome Biology | Full Text
The discovery of RNA interference in the late 1990s [1] prompted a revolution in RNA biology, and the unveiling of small RNA (sRNA)-mediated gene regulatory pathways has profoundly shaped our understanding of the complexity of gene regulation. In eukaryotes, sRNAs have been found to control cellular metabolism, growth and differentiation, to maintain genome integrity, and to combat viruses and mobile genetic elements [2]. These regulatory sRNAs have been classified into at least six groups, including microRNAs (miRNAs), heterochromatic small interfering RNAs (hc-siRNAs), trans-acting small interfering RNAs ...
  https://genomebiology.biomedcentral.com/articles/10.1186/gb-2012-13-6-r47
*  TGF-beta 1 is an autocrine-negative growth regulator of human colon carcinoma FET cells in vivo as revealed by transfection of...
Transforming growth factor-beta 1 (TGF-beta 1) has previously been implicated as a potential negative autocrine or paracrine growth regulator of certain cell types (Arteaga, C. L., R. J. Coffey, Jr., T. C. Dugger, C. M. McCutchen, H. L. Moses, and R. M. Lyons. 1990. Cell Growth & Differ. 1:367-374; Hafez, M. M., D. Infante, S. Winawer, and E. Friedman. 1990. Cell Growth & Differ. 1:617-626; Glick, A. B., K. C. Flanders, D. Danielpour, S. H. Yuspa, and M. B. Sporn. 1989. Cell Regulation. 1:87-97). This is based mainly on experiments assessing the effects of exogenous TGF-beta 1 or neutralizing antibodies to TGF-beta 1 on normal or tumor cell proliferation in vitro. However, direct evidence demonstrating such a negative regulation of tumor cell growth in vivo is still lacking. To overcome this problem we have constructed and used an antisense expression vector for TGF-beta 1 as a means of regulating endogenous TGF-beta 1 expression in tumor cells. Antisense-transfected FET ...
  http://jcb.rupress.org/content/116/1/187
*  WikiGenes - ATP6V1B1-AS1 - ATP6V1B1 antisense RNA 1
The world's first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.
  https://www.wikigenes.org/e/gene/e/101927750.html
*  WikiGenes - SRD5A3-AS1 - SRD5A3 antisense RNA 1
The world's first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.
  https://www.wikigenes.org/e/gene/e/100506462.html
*  WikiGenes - NPHP3-AS1 - NPHP3 antisense RNA 1
The world's first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.
  https://www.wikigenes.org/e/gene/e/348808.html
*  WikiGenes - LIMD1-AS1 - LIMD1 antisense RNA 1
The world's first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.
  https://www.wikigenes.org/e/gene/e/644714.html