*  Aspartate carbamoyltransferase - Wikipedia
... the condensation of l-aspartate and carbamoyl phosphate to form N-carbamyl-L-aspartate and inorganic phosphate. ATCase controls ... and the carbamoyl phosphate domain, which contains most of the residues that bind to carbamoyl phosphate. Each regulatory ... These residues are located at the cleft between the carbamoyl phosphate and aspartate domains at the C1-C4 interface. The ... Gouaux JE, Lipscomb WN (Jun 1988). "Three-dimensional structure of carbamoyl phosphate and succinate bound to aspartate ...
*  aspartate carbamoyltransferase(EC - Creative Enzymes
... also known as aspartate transcarbamoylase or ATCase) catalyzes the first step in the pyrimidine biosynthetic pathway. ATCase is ... the condensation of l-aspartate and carbamoyl phosphate to form N-carbamyl-L-aspartate and inorganic phosphate. ... L-aspartate transcarbamoylase; L-aspartate transcarbamylase; carbamoylaspartotranskinase; aspartate transcarbamylase; aspartate ... Aspartate carbamoyltransferase (also known as aspartate transcarbamoylase or ATCase) catalyzes the first step in the pyrimidine ...
*  Transferase - Wikipedia
In ATCase such a transfer is written as Carbamyl phosphate + L-aspertate → {\displaystyle \rightarrow } L-carbamyl aspartate + ... Carbamoyltransferases, as an example, transfer a carbamoyl group from one molecule to another. Carbamoyl groups follow the ... glyceraldehyde 3-phosphate ⇌ {\displaystyle \rightleftharpoons } erythrose 4-phosphate + fructose 6-phosphate. Transfer of acyl ... Reichard P, Hanshoff G (1956). "Aspartate Carbamyl Transferase from Escherichia Coli" (PDF). Acta Chemica Scandinavica: 548-566 ...
*  Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (IPR005479) | InterPro | EMBL-EBI
Carbamyl phosphate synthetase III, an evolutionary intermediate in the transition between glutamine-dependent and ammonia- ... CPSase II occurs as part of a multi-enzyme complex along with aspartate transcarbamoylase and dihydroorotase; this complex is ... Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (IPR005479) *Carbamoyl-phosphate synthase large subunit, ... Role of conserved residues within the carboxy phosphate domain of carbamoyl phosphate synthetase.. Biochemistry 35 14352-61 ...
Carbamoyl-phosphate synthase (CPSase) catalyzes the ATP-dependent synthesis of carbamyl-phosphate from glutamine (EC ... the C-terminal part which encompass the dihydroorotase and aspartate transcarbamylase activities. ... one to catalyze the phosphorylation of bicarbonate to carboxy phosphate and the other that of carbamate to carbamyl phosphate. ... Mammalian carbamyl phosphate synthetase (CPS). DNA sequence and evolution of the CPS domain of the Syrian hamster ...
*  nitrogen metabolism
This liver mitochondrial enzyme converts the ammonia produced by glutamate dehydrogenase into carbamyl phosphate (=carbamoyl ... Once in the cytosol, citrulline condenses with aspartate and the reaction is driven by ATP. In this way aspartate contributes ... Carbamyl phosphate synthesis is a major burden for liver mitochondria. This enzyme accounts for about 20% of the total protein ... It should be distinguished from a second cytosolic glutamine-dependent carbamyl phosphate synthetase [CPS2] which is found in ...
*  Compositions and Methods for Modeling Saccharomyces cerevisiae Metabolism - Patent application
CAASP N-Carbamoyl-L-aspartate CAIR 1-(5-Phospho-D-ribosyl)-5-amino- 4-imidazolecarboxylate CALH 2-(3-Carboxy-3-aminopropyl)-L- ... YJR109C CPA2 carbamyl phosphate synthetase, large chain GLN + 2 ATP + CO2 -, GLU + CAP + 2 ADP + cpa2 PI YOR303W 6.3. ... G1P D-Glucose 1-phosphate G6P alpha-D-Glucose 6-phosphate GA1P D-Glucosamine 1-phosphate GA6P D-Glucosamine 6-phosphate GABA 4- ... PHOSPHATE 5-KINASE, 1- phosphatidylinositol-4-phosphate kinase YDR208W MSS4 Phosphatidylinositol-4-phosphate 5-kinase ...
*  Mammalian dihydroorotase: nucleotide sequence, peptide sequences, and evolution of the dihydroorotase domain of the...
... and aspartate transcarbamoylase (carbamoyl-phosphate: L-aspartate carbamoyltransferase, EC activities. We sequenced ... Thus, the order of domains within the polypeptide is NH2-carbamoyl-phosphate synthetase-DHO-aspartate transcarbamoylase-COOH. ... which also has a carbamoyl-phosphate synthetase [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate- ... Substructure of the Amidotransferase Domain of Mammalian Carbamyl Phosphate Synthetase. *Scopus (49) ...
*  Sirt5 Is a NAD-Dependent Protein Lysine Demalonylase and Desuccinylase | Science
SIRT5 Deacetylates carbamoyl phosphate synthetase 1 and regulates the urea cycle. Cell 137, 560 (2009). doi:10.1016/j.cell. ... A kinetic study of carbamyl phosphate synthetase. J. Biol. Chem. 239, 1925 (1964). pmid:14213379. ... and aspartate aminotransferase. The sites of succinyl modification were identified through MS/MS (fig. S13). Furthermore, we ... Sirt5 is known to regulate the activity of carbamoyl phosphate synthase 1 (CPS1) (22). We therefore sought to test whether CPS1 ...
*  WikiGenes - arginine - (2S)-2-amino-5-(diaminomethylideneamino)pe...
Neonatal pulmonary hypertension--urea-cycle intermediates, nitric oxide production, and carbamoyl-phosphate synthetase function ... Unsuccessful therapy of neonatal hyperammonemia with N-carbamyl-L-glutamate and L-arginine. Gelehrter, T.D., Rosenberg, L.E. N ... We demonstrate here the essential function of an aspartate group in the catalysis of phosphoryl transfer by Escherichia coli ... Unsuccessful therapy of neonatal hyperammonemia with N-carbamyl-L-glutamate and L-arginine [24]. ...
*  Carbamyl - definition of carbamyl by The Free Dictionary
carbamyl synonyms, carbamyl pronunciation, carbamyl translation, English dictionary definition of carbamyl. n a radical, NH2CO ... carbamoyl phosphate synthetases. *carbamoyl-phosphate synthase [ammonia], mitochondrial. *carbamoyl-phosphate synthase 1, ... carbamyl aspartate and dihydroorotate accumulate to high levels in an equilibrium ratio of 20.. Antimicrobial properties of ... carbamyl. Also found in: Medical, Encyclopedia, Wikipedia.. Related to carbamyl: carbamoyl, carbamate carbamyl. (ˈkɑːbəmɪl) n. ...
*  Amino-Heterocyclic Compounds - Patent application
0047] The terms "carbamoyl" and "carbamyl" denote an amino group (--NR'R'') bonded to a carbonyl group (C═O) that is attached ... 8-3H]guanosine 3',5'-cyclic phosphate (TRK 392, GE Healthcare Life Sciences) was diluted in assay buffer (50 mM Tris-HCl, pH7.5 ... 0098] (xxi) N-methyl-D-aspartate (NMDA) receptor antagonists, such as memantine (NAMENDA, AXURA, EBIXA), amantadine (SYMMETREL ... 0183] C. Preparation of (1S)-2-[(4-carbamoyl-1-cyclopentyl-1H-pyrazol-5-yl)amino]-1-methyl-2-oxoe- thyl acetate (C30). (1S)-2- ...
*  Agmatine - Scientific Review on Usage, Dosage, Side Effects | Examine.com
If the latter pathway is chosen and carbamoyl phosphate is produced, it can be metabolized into carbamate via the carbamate ... Reynolds IJ. Arcaine uncovers dual interactions of polyamines with the N-methyl-D-aspartate receptor. J Pharmacol Exp Ther. ( ... TIGIER H, GRISOLIA S. INDUCTION OF CARBAMYL-P SPECIFIC OXAMATE TRANSCARBAMYLASE BY PARABANIC ACID IN A STREPTOCOCCUS. Biochem ... The above mechanism is common to transcarbomylase enzymes (that produce carbamoyl phosphate), as both ornithine ...
*  NOVEL INHIBITORS OF RHO KINASE - Patent application
"Phosphonate" includes "phosphate [(OH)2P(O)O--] and related phosphoric acid anions which may form salts. [0069]The terms " ... 0037]The term "O-carbamyl" as used herein, alone or in combination, refers to a --OC(O)NRR', group-with R and R' as defined ... 0025]The terms "amido" and "carbamoyl," as used herein, alone or in combination, refer to an amino group as described below ... Representative acid addition salts include acetate, adipate, alginate, L-ascorbate, aspartate, benzoate, benzenesulfonate( ...
*  A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N...
Abbreviations: NAGP, N-acetylglutamyl phosphate; NAGPR, N-acetylglutamyl phosphate reductase; AOAT, acetylornithine ... L-glutamine or L-aspartate. The amounts of acetyl-D-glutamate, acetyl-L-glutamine or acetyl-L-aspartate formed were measured ... In mammals, amphibians, fish and some invertebrates, NAGS, carbamylphosphate synthase, together with the last four enzymes of ... Lawson FS, Charlebois RL, Dillon JA: Phylogenetic analysis of carbamoylphosphate synthetase genes: complex evolutionary history ...
*  Preparation of Saturated Ketone Morphinan Compounds by Catalytic Isomerization - Patent application
0038]In preferred embodiments, Z is selected from the group consisting of acetate, aspartate, benzoate, bitartrate, citrate, ... These substituents include alkyl, alkoxy, acyl, acyloxy, alkenyl, alkenoxy, aryl, aryloxy, amino, amido, acetal, carbamyl, ... Crystal of 6,7-unsaturated-7-carbamoyl morphinan derivative and method for producing the same. ... phosphate, phthalate, propionate, pyruvate, salicylate, stearate, succinate, sulfate, tannate, tartrate, terephthalate, ...
*  Kinetic Mechanism
ATCase catalyzes the reaction of aspartate with carbamyl-P to produce carbamylaspartate in the first step of pyrimidine ... Carbamoyl Phosphate Synthetase. *Carbanionic Mechanisms. *Carbocationic Mechanisms. *Carboxylation Mechanism. *Caspases. * ... An early example of the binding of a two-substrate analog was the inhibition of aspartate transcarbamylase (ATCase) by N-( ... and aspartate a-decarboxylases. Pyruvoyl-enzymes are found in both prokaryotes and eukaryotes. d-Proline reductase is also a ...
0350]Kinder, D. H., Frank, S. K., Ames, M. M. (1990) Analogues of Carbamyl Aspartate as Inhibitors of Dihydrooratase: ... bringing the γ-phosphate of ATP and the Ser OH in closer proximity for direct transfer of the phosphate. [0011]However, many ... N-(1-carbamoyl-2-methylbutyl)(5-hydroxyindol-2-yl)carboxyamide (7) [0270]100 mg (0.56 mmol) 5-hydroxy-2-indolecarboxylic acid, ... proposed to interact with the γ-phosphate of ATP to help catalyze transfer of this phosphate. Two additional important PKA ...
*  Citrulline synthesis in rat tissues and liver content of carbamoyl phosphate and ornithine | Biochemical Journal
Rat liver ornithine carbamoyltransferase appears to be located exclusively in the mitochondria; the activity that is found in the soluble fraction is indistinguishable from mitochondrial ornithine carbamoyltransferase by simple kinetic criteria, and seems to result from breakage of mitochondria during homogenization. Of several rat tissues studied, only the liver and the mucosa of small intestine contain significant amounts of ornithine carbamoyltransferase; the activity in intestinal mucosa is less than one thousandth of that in liver. Qualitatively, this distribution coincides with that of carbamoyl phosphate synthetase I and its cofactor, acetylglutamate. The rat liver contents of carbamoyl phosphate and ornithine were 0.1 and 0.15μmol/g wet wt. of tissue respectively. On the basis of these values, it is proposed that in vivo the ornithine ...
*  The Toxoplasma Blog: Genetic identification of essential indels and domains in carbamoyl phosphate synthetase II
New treatments need to be developed for the significant human diseases of toxoplasmosis and malaria to circumvent problems with current treatments and drug resistance. Apicomplexan parasites causing these lethal diseases are deficient in pyrimidine salvage, suggesting that selective inhibition of de novo pyrimidine biosynthesis can lead to a severe loss of uridine 5'-monophosphate (UMP) and thymidine 5'-monophosphate (dTMP) pools, thereby inhibiting parasite RNA and DNA synthesis. Disruption of Toxoplasma gondii carbamoyl phosphate synthetase II (CPSII) induces a severe uracil auxotrophy with no detectable parasite replication in vitro and complete attenuation of virulence in mice. Here we show that a CPSII cDNA minigene efficiently complements the uracil auxotrophy of CPSII-deficient mutants, restoring parasite growth and virulence. Our complementation assays reveal that engineered ...
*  Toxicology and Cancer Biology
A study of the sulfhydryl groups of the catalytic subunit of Escherichia coli aspartate transcarbamylase. The use of enzyme--5-thio-2-nitrobenzoate mixed disulfides as intermediates in modifying enzyme sulfhydryl groups ...
PRINCIPLE: Enzymatic Assay of ORNITHINE CARBAMYL TRANSFERASE Carbamyl phosphate + L-Ornithine OCT > L-Citrulline + P i Abbreviations: P i = Inorganic Phosphate OCT = Ornithine Carbamyl Transferase CONDITIONS:
*  Unikont - Wikipedia
The unikonts have a triple-gene fusion that is lacking in the bikonts. The three genes that are fused together in the unikonts, but not bacteria or bikonts, encode enzymes for synthesis of the pyrimidine nucleotides: carbamoyl phosphate synthase, dihydroorotase, aspartate carbamoyltransferase. This must have involved a double fusion, a rare pair of events, supporting the shared ancestry of Opisthokonta and Amoebozoa.. Cavalier-Smith[1] originally proposed that unikonts ancestrally had a single flagellum and single basal body. This is unlikely, however, as flagellated opisthokonts, as well as some flagellated Amoebozoa, including Breviata, actually have two basal bodies, as in typical 'bikonts' (even though only one is flagellated in most unikonts). This paired arrangement can also be seen in the organization of centrioles in typical animal cells. In spite of the name of the group, the common ancestor of all 'unikonts' was ...
*  Rate limiting enzymes - PgAspirant.in
Rate limiting enzymes Glycolysis- Phosphofructokinase 1f Gluconeogenesis- Fructose 1,6 bisphosphatase Glycogen synthesis- Glycogen synthase Glycogenolysis-Glycogen Phosphorylase Fatty acid synthesis- AcetylCoA Carboxylase Fatty acid beta oxidation-Carnitine acyl transferase 1 Lipolysis- hormone sensitive lipase Purine metabolism- PRPP Amidotransferase Pyrimidine metabolism- Aspartate transacetylase Ketone body synthesis-HMG CoA synthase Cholesterol synthesis- HMG CoA resuctase Bile acid synthesis- 7 Alpha hydroxylase Uric acid synthesis- xanthine oxidase Catecholamine synthesis- Tyrosine hydroxylase Urea cycle- Carbamoyl Phosphate synthase 1 Pentose phosphate pathway- Glucose-6-Phosphate dehydrogenase Kreb's- Isocitrate dehydrogenase Adrenal hormones- Desmolase Porphyrin/Haem synthesis- ALA synthase Postaglandin synthesis- PG ...
*  The effects L-leucine on the synthesis of urea, glutamate and glutamine by isolated rat liver cells | Biochemical Journal
With either alanine or a mixture of 15 different amino acids as nitrogen source, the addition of L-leucine inhibited the synthesis of urea by isolated rat liver cells. With alanine present leucine promoted the production of glutamate and glutamine. Comparison of effects of leucine on soluble glutamate dehydrogenase, mitochondria and isolated cells supports the postulate that leucine exerts its effect through activation of glutamate dehydrogenase. It is suggested that this latter enzyme may not be as important for the production of NH3 for carbamoyl phosphate synthesis as has been considered hitherto.. ...
*  Carbamoyl-phosphate synthase [ammonia], mitochondrial
The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.[provided by RefSeq, May 2010 ...
*  Ornithine Carbamoyltransferase Proteins: Novus Biologicals
Ornithine Carbamoyltransferase Proteins available through Novus Biologicals. Browse our Ornithine Carbamoyltransferase Protein catalog backed by our Guarantee+.
*  Talk:Carbamoyl phosphate synthetase I - Wikipedia
CPS I does not transfer ammonia from glutamine. This is done by CPS II. The ammonia source for CPS I is glutamat. Its an enzyme with a channel for free ammonia. Ammonia never leaves the enzym.. "CPSI is allosterically regulated by purine and pyrimidine nucleotides where the former is allosterically activates the enzyme and the later allosterically inhibits CPSI." ,--Is this true? I thought this was for CPSII? N- Acetylglutamate is the most important allosteric regulator of CPSI (http://www.biochemj.org/bj/424/bj4240211.htm). -Preceding unsigned comment added by (talk) 17:37, 7 February 2011 (UTC). ...
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
*  carbamyl plasmin A Summary Report | CureHunter
carbamyl plasmin A: an active enzyme with a single free NH(2)-terminal amino group(Val-561); derivative of EC, fibrinolysin
*  Ornithine Carbamoyltransferase Antibody (NBP1-88121): Novus Biologicals
Rabbit Polyclonal Anti-Ornithine Carbamoyltransferase Antibody. Validated: IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat. 100% Guaranteed.
*  Three serendipitous pathways in E. coli can bypass a block in pyridoxal‐5′‐phosphate synthesis | Molecular Systems Biology
The experiments described here reveal the existence of three serendipitous pathways that allow synthesis of PLP in the ΔpdxB strain when any one of seven different genes is overexpressed. The number of genes that allow complementation is surprising; most multicopy suppression experiments reveal fewer genes that can complement a strain lacking a metabolic enzyme. For example, Patrick et al (2007) found that 21 of 104 knockout strains of E. coli could be complemented by multicopy suppression using the ASKA library, but in most cases by only one or two genes. One exception, the ΔglyA strain, was complemented by four genes, one of which encodes an antisigma factor. A second unusual case was described by Miller and Raines (2004, 2005), who found that overexpression of four genes encoding glycokinases with promiscuous glucokinase activity complemented a strain lacking glucokinase. Our finding that seven different genes complement the ΔpdxB strain is, to our knowledge, the record. Furthermore, our ...
*  Nobel::1953 - Fritz A. Lipmann
Fritz Albert Lipmann (1899 -1986) was awarded half of the 1953 Nobel Prize in Physiology or Medicine for his discovery of coenzyme A and its importance for intermediary metabolism, in which energy is extracted from cellular nutrients and used to build cellular components. His early work included the identification of serine phosphate as the constituent of phosphoproteins, a group of proteins which are chemically bonded to a substance containing phosphoric acid, and an investigation into the Pasteur effect, which showed that oxygen inhibits fermentation in yeast and led to important discoveries on the mechanism of this reaction and on the role of glycolysis in the metabolism of cells in embryos. Lipmann's initial work on coenzyme A led him to investigate the role of phosphorylation in the intermediary reactions of biosynthesis. His later research included demonstrating that carbamoyl phosphate - a molecule involved in ...
*  anti-CAD antibody | GeneTex
CAD antibody (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) for ICC/IF, WB. Anti-CAD pAb (GTX28406) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
8AT1: Crystal structures of aspartate carbamoyltransferase ligated with phosphonoacetamide, malonate, and CTP or ATP at 2.8-A resolution and neutral pH.
*  Protein Lounge: Synthesis of Pyrimidine Ribonucleotides
|P>Synthesis of the Pyrimidines is less complex than that of the Purines, since the base is much simpler. Synthesis of Carbamoyl-P (Carbamoyl Phosphate) is the first reaction of Pyrimidine biosynthesis. Carbamoyl-P is formed from HCO3- (Bicarbonate) and the amide nitrogen [...]
*  PQR | N-{2-benzyl-4-[(methylsulfonyl)carbamoyl]phenyl}-6-(cyclohexylmethyl)-2-pyridinecarboxamide
You are viewing an interactive 3D depiction of the molecule n-{2-benzyl-4-[(methylsulfonyl)carbamoyl]phenyl}-6-(cyclohexylmethyl)-2-pyridinecarboxamide (C28H31N3O4S) from the PQR.
*  PQR | O-[(4-methyl-2-oxo-2h-chromen-7-yl)carbamoyl]-l-serine
You are viewing an interactive 3D depiction of the molecule o-[(4-methyl-2-oxo-2h-chromen-7-yl)carbamoyl]-l-serine (C14H15N2O6+) from the PQR.
*  Patent US7516568 - Calendar - Google Patents
A calendar is formed of a plurality of substrates. A first substrate carries indicia thereon which identifies selected time periods, such as days or months of the year. A second substrate is positioned adjacent to the first substrate. The second substrate defines a plurality of cavities dimensioned to individually retain a respective information carrying article, such as a web. Each of the cavities is corresponding supplied with a respective information carrying article. Each indicia on the first substrate is positionally associated with a respective cavity in the second substrate. A third substrate, positioned adjacent to the second substrate, is positioned to retain the information carrying articles releasably within the second substrate. The third substrate provides a rupturable cover over each of the cavities of the second substrate whereby upon the application of a sufficient lateral force on the information carrying article within a selected cavity, the article passes through the cover to a
4) Manually examined on the 1247 genes to validate it was acutally related to CHD. Some genes would be filtered if it represents other meanings (such as gene CAD, Entrez ID:790, carbamoyl-phosphate synthetase 2, is mostly meant coronary arterial disease in articles). 681 genes were then validated with at least one reference ...
*  Hexasodium 4-hydroxy-7-[({5-hydroxy-7-sulfonato-6-[(E)-{2-sulfonato-4-[(E)-(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]-2...
Hexasodium 4-hydroxy-7-[({5-hydroxy-7-sulfonato-6-[(E)-{2-sulfonato-4-[(E)-(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]-2-naphthyl}carbamoyl)amino]-3-[(E)-{2-sulfonato-4-[(E)-(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]-2- ...