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*  Targeted Metabolic Imaging to Improve the Management of Heart Disease | JACC: Cardiovascular Imaging
... the balance between cellular metabolism and contractile function is perturbed. The decrease in coronary flow and concomitant ... To assess fatty acid use, earlier studies used the labeled substrate carbon C 11 (11C)-palmitate. After its uptake into the ... the resultant energy deficit ultimately leads to cell death, either by apoptosis or necrosis (14). Hence, acute and chronic ... The function of amino acids as building blocks for proteins and the intermediary metabolism of amino acids in the body was ...
  http://imaging.onlinejacc.org/content/5/2/214
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, ... Mitochondria have roles in many cellular processes, from energy metabolism and calcium homeostasis to control of cellular ... These methods allow for a selective activation/inhibition of individual PKC isozymes to assess their role in cellular functions ... Cellular Biology, Issue 50, Ratiometric pericam, mitochondria, calcium, apoptosis, staurosporine, live cell imaging ...
  https://www.jove.com/visualize/abstract/20548781/polymorphisms-mitochondrial-dna-control-region-frailty-older
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, ... Mitochondria have roles in many cellular processes, from energy metabolism and calcium homeostasis to control of cellular ... Cellular Biology, Issue 50, Ratiometric pericam, mitochondria, calcium, apoptosis, staurosporine, live cell imaging ... Methods to Assess Subcellular Compartments of Muscle in C. elegans. Authors: Christopher J. Gaffney, Joseph J. Bass, Thomas F. ...
  https://www.jove.com/visualize/abstract/23520503/metabolic-adaptation-to-chronic-inhibition-mitochondrial-protein
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, ... Here we describe optimization of a multiplex assay to assess apoptosis following a palmitic acid (PA) challenge in an in vitro ... Mitochondria have roles in many cellular processes, from energy metabolism and calcium homeostasis to control of cellular ... Our recent data demonstrate that PKC regulates mitochondrial function and cellular energy status. Numerous reports demonstrated ...
  https://www.jove.com/visualize/abstract/22586466/overfeeding-reduces-insulin-sensitivity-increases-oxidative-stress
*  Cardiac Metabolism Imaging and Chemotherapy Cardiotoxicity | OMICS International
Carbohydrates metabolism. The heart can function with 10 to 40% of its energy derived from pyruvate oxidation (glycolysis and ... This false long-chain FFA is blocked by its sulfur component, and cellular uptake represents both β-oxidation and ... Medical imaging has been used extensively to diagnose heart failure and assess cardiac function. Cardiac structure and function ... which in turn induces apoptosis and the byproduct, nitric oxide, involved in endothelial function [64,65]. Furthermore, ...
  https://www.omicsonline.org/open-access/cardiac-metabolism-imaging-and-chemotherapy-cardiotoxicity-2161-0495-1000213.php?aid=67039
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Mitochondria have roles in many cellular processes, from energy metabolism and calcium homeostasis to control of cellular ... deoxyglucose to assess tissue-specific glucose uptake and [3-3H]glucose to assess the ability of insulin to suppress the rate ... Our recent data demonstrate that PKC regulates mitochondrial function and cellular energy status. Numerous reports demonstrated ... RPTC viability is assessed using annexin V/propidium iodide staining followed by flow cytometry to determine apoptosis and ...
  https://www.jove.com/visualize/abstract/22028857/changes-cardiac-substrate-transporters-metabolic-proteins-mirror
*  Protocols and Video Articles Authored by Jen-Tsan Chi (Translated to Spanish)
Acidosis Induces Reprogramming of Cellular Metabolism to Mitigate Oxidative Stress Cancer & Metabolism. 2013 , Pubmed ID: ... We also provide evidence that knockdown of NFYB enhances E2F1-induced apoptosis, suggesting a pro-survival function of the NFYB ... Here, we demonstrate that PGC-1α, a central regulator of energy metabolism, is suppressed in VHL-deficient ccRCC by a HIF/Dec1- ... Kinetic uptake rates of lactate in vivo surpassed that of glucose in R3230Ac mammary carcinomas. The uptake appeared specific ...
  https://www.jove.com/author/Jen-Tsan_Chi?language=Spanish
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Mitochondria have roles in many cellular processes, from energy metabolism and calcium homeostasis to control of cellular ... Our recent data demonstrate that PKC regulates mitochondrial function and cellular energy status. Numerous reports demonstrated ... These methods allow for a selective activation/inhibition of individual PKC isozymes to assess their role in cellular functions ... Furthermore, since cation uptake is determined on single cells, the flux experiments can be carried out in combination with two ...
  https://www.jove.com/visualize/abstract/22870326/the-effect-gonadotropin-on-glucose-transport-apoptosis-rat
*  Protocols and Video Articles Authored by Filippo Rossi (Translated to Italian)
The uptake mechanisms of PMMA-NPs were in-depth investigated, together with their possible toxic effects on microglia/ ... Sixteen female piglets were studied to assess the effect of sorbic acid supplementation on the small intestine tissue ... Research on the interaction among nutrients, gut microbiota and host metabolism is presently unravelling the molecular ... is involved in the direct signaling of cell death by Abeta via the function of its death domain. This signaling leads to the ...
  https://www.jove.com/author/Filippo_Rossi?language=Italian
*  Cardiomyocyte ATP Production, Metabolic Flexibility, and Survival Require Calcium Flux through Cardiac Ryanodine Receptors in...
Our data indicate that disruption of RYR2 function, via its effects on mitochondrial calcium signaling and cellular metabolism ... 3. Liu T., O'Rourke B. (2008) Enhancing mitochondrial Ca2+ uptake in myocytes from failing hearts restores energy supply and ... Paraformaldehyde-fixed ventricular tissue sections were stained with H&E and Masson's trichrome to assess fibrosis. Apoptosis ... Ideally, the hypothesis that intracellular Ca2+ release is required to sustain energy metabolism in vivo would be tested using ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3696672/
*  Protocols and Video Articles Authored by Werner J. H. Koopman
... sustains organelle function and plays a central role in cellular energy metabolism. The OXPHOS system consists of 5 ... Next, we discuss the interactions between cellular glucose metabolism and ROS. ROS-stimulated cellular glucose uptake can ... Mitochondria are crucially involved in cellular Ca2+ and redox homeostasis and apoptosis induction. Maintenance of ... cellular ROS levels is a challenging task. In this chapter we describe two strategies to measure ROS: one approach to assess ...
  https://www.jove.com/author/Werner++J.+H._Koopman
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, ... Our recent data demonstrate that PKC regulates mitochondrial function and cellular energy status. Numerous reports demonstrated ... These methods allow for a selective activation/inhibition of individual PKC isozymes to assess their role in cellular functions ... Cellular Biology, Issue 50, Ratiometric pericam, mitochondria, calcium, apoptosis, staurosporine, live cell imaging ...
  https://www.jove.com/visualize/abstract/19557129/linoleic-acid-induced-mitochondrial-ca-2-efflux-causes-peroxynitrite
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, ... Mitochondria have roles in many cellular processes, from energy metabolism and calcium homeostasis to control of cellular ... Methods to Assess Subcellular Compartments of Muscle in C. elegans. Authors: Christopher J. Gaffney, Joseph J. Bass, Thomas F. ... We have reported selective induction of apoptosis in cancer cells by the natural compound pancratistatin (PST). Recently, a ...
  https://www.jove.com/visualize/abstract/23626747/penetrating-cations-enhance-uncoupling-activity-anionic-protonophores
*  Protocols and Video Articles Authored by K. George Chandy (Translated to Russian)
The obesity diet induced Kv1.3 expression in the liver, and ShK-186 caused profound alterations in energy and lipid metabolism ... Our results provide clues to the structure and function of the vast family of proteins that contain domains related to sea ... These results were compared with SPECT/CT data collected with an (111)In-DOTA-conjugate of ShK-186 to assess whole blood ... ShK-186 therapy activated brown adipose tissue as evidenced by a doubling of glucose uptake, and increased β-oxidation of fatty ...
  https://www.jove.com/author/K.+George_Chandy?language=Russian
*  Plus it
One way to assess this is to measure the aconitase activity because the function of this mitochondrial enzyme critically ... Carvajal K, Banos G, Moreno-Sanchez R: Impairment of glucose metabolism and energy transfer in the rat heart. Mol Cell Biochem ... 6A). We also measured the citrate synthase activity, which is frequently used to assess the cellular mitochondrial content, and ... Listenberger LL, Ory DS, Schaffer JE: Palmitate-induced apoptosis can occur through a ceramide-independent pathway. J Biol Chem ...
  http://diabetes.diabetesjournals.org/content/56/4/1136
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, ... Mitochondria have roles in many cellular processes, from energy metabolism and calcium homeostasis to control of cellular ... These methods allow for a selective activation/inhibition of individual PKC isozymes to assess their role in cellular functions ... RPTC viability is assessed using annexin V/propidium iodide staining followed by flow cytometry to determine apoptosis and ...
  https://www.jove.com/visualize/abstract/22194845/galactose-enhances-oxidative-metabolism-reveals-mitochondrial
*  The Use of Anti-Diabetic Drugs in Alzheimer's Disease, New Therapeutic Options and Future Perspective
Thus, it has been shown that an increase in OS can lead to further inhibition of the production of cellular energy and ... AMPK plays an important role in the regulation of intracellular event such as glucose uptake, metabolism of lipids and ... the motor function of the brain and obesity indicating that the deterioration of cognitive function can increase with high body ... Based on the shared pathophysiology between diabetes and AD, several studies have been conducted to assess the neuroprotective ...
  http://www.scirp.org/journal/PaperInformation.aspx?PaperID=85437
*  http://ufdc.ufl.edu/UFE0042452/00001
Thus it is not feasible to use these inhibitors at high concentration to assess CAIX function. At low concentrations of the AE ... One of striking features of tumor cells is the shift of their metabolism toward glycolysis, which is less efficient in energy ... Uptake of 3 O methyl D [1 3 H ] glucose into the cells reaches a plateau after 40 50 min an d remains constant for up to at ... 79 Sub cellular localization of the non specific protein(s) CAIX is a transmembrane protein and well recognized as a hypoxia ...
  http://ufdc.ufl.edu/UFE0042452/00001
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, ... This method presents how to assess the differential contribution of the energy systems in sports that are difficult to mimic in ... We have reported selective induction of apoptosis in cancer cells by the natural compound pancratistatin (PST). Recently, a ... Mitochondria have roles in many cellular processes, from energy metabolism and calcium homeostasis to control of cellular ...
  https://www.jove.com/visualize/abstract/21541279/amyloid-beta-resistance-nerve-cell-lines-is-mediated-warburg
*  Frontiers | The impact of vitamin D in breast cancer: genomics, pathways, metabolism | Physiology
... apoptosis and differentiation. New areas of emphasis include regulation of tumor metabolism and innate immune responses. ... New areas of emphasis include regulation of tumor metabolism and innate immune responses. However, the role of VDR in ... However, tissue uptake and cellular metabolism of these metabolites in vivo is likely to be highly relevant to cancer biology, ... attention has recently been re-focused on the role of cellular energy metabolism as a critical component of tumor initiation ...
  https://www.frontiersin.org/articles/10.3389/fphys.2014.00213/full
*  Bioassays and Preclinical Studies | Brunswick Labs
Energy metabolism investigation impact of a material on cellular and mitochondrial potential, oxidative phosphorylation, ... Anti-cancer Function evaluation: cancer cell viability, proliferation, apoptosis, and invasion as biomarkers ... Diabetes and weight management insulin secretion, insulin receptor activity, glucose transport, uptake, and metabolism, and ... As a step forward from chemistry "test tube" testing, we provide biological assays to assess the health effects of a material ...
  https://brunswicklabs.com/capabilities/bioassays/
*  Haemodynamic response - Wikipedia
As a result, cellular energy failure, depolarization of neuronal and glial membranes, edema, and excess neurotransmitter and ... can help assess the effects of stroke and other degenerative diseases such as Alzheimer's disease on brain function. Another ... NE uptake leads to an increase in intracellular astrocyte Ca2+. When these calcium ion waves spread down the length of the ... This ultimately ends with cell death, as cells succumb to a lack of nutrients to power their metabolism and to a toxic brain ...
  https://en.wikipedia.org/wiki/Haemodynamic_response
*  Microbial cooperation - Wikipedia
These molecules are known as chelating agents and play an important role in facilitating the uptake and metabolism of iron in ... Structure and function of microbial iron transport compounds. J. Biol. Chem. 270:26723-6. 7. doi: 10.1074/jbc.270.45.26723 West ... Apoptosis in yeast-mechanisms and ben- efits to a unicellular organism. Mol. Microbiol. 62:1515-21. Charlesworth B. 1978. Some ... In the case of siderophore production, there must be equilibrium between the microbes that spend their energy to produce the ...
  https://en.wikipedia.org/wiki/Microbial_cooperation
*  Biochemical cascade - Wikipedia
... may disrupt neuronal energy metabolism and ion homeostasis, by impairing the function of membrane ion-motive ATPases, glucose ... This kinase is involved in cell survival and inhibition of apoptosis, cellular growth and maintenance of pluripotency, in ... Maere, S (2005). "BiNGO: a Cytoscape plugin to assess overrepresentation of Gene Ontology categories in biological networks". ... mitochondrial dysfunction and impaired glucose uptake in vulnerable neuronal populations. Studies of animal and cell culture ...
  https://en.wikipedia.org/wiki/Biochemical_cascade
*  MicroRNA - Wikipedia
Consistent with the profiling results, ectopic apoptosis was observed in the cellular derivatives of the FoxD1 derived ... The function of miRNAs appears to be in gene regulation. For that purpose, a miRNA is complementary to a part of one or more ... Exportin-5-mediated transport to the cytoplasm is energy-dependent, using GTP bound to the Ran protein. In the cytoplasm, the ... During miRNA maturation in the cytoplasm, uptake by the Argonaute protein is thought to stabilize the guide strand, while the ...
  https://en.wikipedia.org/wiki/MicroRNA