*  Cytochrome c - Wikipedia
Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor-1 (Apaf-1).[5] ... This release of cytochrome c in turn activates caspase 9, a cysteine protease. Caspase 9 can then go on to activate caspase 3 ... The release of cytochrome-c from mitochondria to the cytosol, where it activates the caspase family of proteases is believed to ... It belongs to the cytochrome c family of proteins. Cytochrome c is highly water-soluble, unlike other cytochromes, and is an ...
  https://en.wikipedia.org/wiki/Cytochrome_C
*  Apaf1 - Apoptotic protease-activating factor 1 - Mus musculus (Mouse) - Apaf1 gene & protein
Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. ... Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 ( ... Oligomerizes upon binding of cytochrome c and dATP.UniRule annotation. ,p>Information which has been generated by the UniProtKB ... Apoptotic protease-activating factor 1UniRule annotation. ,p>Information which has been generated by the UniProtKB automatic ...
  http://www.uniprot.org/uniprot/G3XA09
*  Caspase 9: The Suicidal Cell Whisperer | Antibody News: Novus Biologicals
... by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease ... Their data indicates that the association between apoptotic protease activating factor-1 (Apaf-1) and cytochrome C directly ... The Caspase 9 antibody was used by Krajewski's group to characterize caspase 9 mitochondrial release during cytochrome c- ... Activated caspase 9 primary signaling occurs through caspase 3, which it cleaves and activates. ...
  https://www.novusbio.com/antibody-news/antibodies/caspase-9-the-suicidal-cell-whisperer
*  Reactive oxygen species - Wikipedia
... or apoptotic protease activating factor-1, which is free-floating in the cell's cytoplasm. Using energy from the ATPs in the ... causing cytochrome C to leak out. This cytochrome C binds to Apaf-1, ... ROS then activate various transcription factors such as nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB ... In the intrinsic pathway, ROS function to facilitate cytochrome c release by activating pore-stabilizing proteins (Bcl-2 and ...
  https://en.wikipedia.org/wiki/Reactive_oxygen_species
*  Differential Regulation and ATP Requirement for Caspase-8 and Caspase-3 Activation during CD95- and Anticancer Drug-induced...
... cytochrome c interacts with apoptotic protease-activating factor 1 (Apaf-1), the mammalian homologue of the Caenorhabditis ... 1997) Cytochrome c and dATP-dependent formation of Apaf-1/Caspase-9 complex initiates an apoptotic protease cascade. Cell 91: ... c from mitochondria and the subsequent ATP-dependent activation of the death regulator apoptotic protease-activating factor 1 ( ... A redistribution of cytochrome c into the cytosol is observed in a variety of apoptotic conditions, such as CD95 ligation, or ...
  http://jem.rupress.org/content/188/5/979
*  Plus it
In the cytochrome c/apoptotic protease activating factor-1 (Apaf-1)/caspase-9 pathway, Smac released from the mitochondria ... Smac promotes caspase-9 activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to the IAP and removing their ... A, JNK is activated (phosphorylated) in a time-dependent manner by Aβ. Neuron cultures were treated with 25 μm Aβ25-35 for the ... Carson JP, Behnam M, Sutton JN, Du C, Wang X, Hunt DF, Weber MJ, Kulik G (2002) Smac is required for cytochrome c-induced ...
  http://www.jneurosci.org/content/25/5/1149
*  Xiaodong Wang (biochemist) - Wikipedia
This allowed the biochemical purification of a complex of Cytochrome c, Caspase-9 and the Apoptotic Protease Activating factor- ... APAF1). These components are essential for forming a ternary complex called the apoptosome that activates Caspase-3 downstream ... Zou, H.; Henzel, W. J.; Liu, X.; Lutschg, A.; Wang, X. (1997-08-08). "Apaf-1, a human protein homologous to C. elegans CED-4, ... participates in cytochrome c-dependent activation of caspase-3". Cell. 90 (3): 405-413. doi:10.1016/s0092-8674(00)80501-2. ISSN ...
  https://en.wikipedia.org/wiki/Xiaodong_Wang_(biochemist)
*  Plus it
... cytochrome c binds the docking molecule apo-ptotic protease activating factor 1 (Apaf-1), which then acquires the ability to ... In the case of fludarabine, for example, phosphorylated metabolites were shown to directly activate the cytochrome c/Apaf-1/ ... drug-induced apoptosis is markedly inhibited by targeted deletion of genes encoding apoptotic protease activating factor 1 ( ... factors that regulate activation of the apoptotic machinery might play a more important role than variations in the apoptotic ...
  http://clincancerres.aacrjournals.org/content/10/20/6807
*  The Proapoptotic Effect of Traditional and Novel Nonsteroidal Anti-Inflammatory Drugs in Mammalian and Yeast Cells
Furthermore, deletion of apoptotic protease-activating factor-1 (APAF-1), a cytosolic molecule which mediates caspase 9 ... Truncated Bid (tBid) can translocate to the mitochondria to trigger cytochrome c release [94, 95] and can also activate Bax [96 ... Implications for tumor necrosis factorapoptotic signaling," Journal of Biological Chemistry, vol. 276, no. 15, pp. 12035- ... These observations indicate that cytochrome c release is a critical mediatory mechanism of apoptotic cell death induced by ...
  https://www.hindawi.com/journals/omcl/2013/504230/
*  Mitofusin 2 Triggers Vascular Smooth Muscle Cell Apoptosis via Mitochondrial Death Pathway | Circulation Research
... and it involves sequentially the release of cytochrome c, the recruitment of apoptotic protease activating factor-1 (Apaf-1), ... Overexpression of rMfn-2 Activates Mitochondrial Apoptotic Pathway. To discriminate which apoptotic pathway was responsible for ... There are 2 major apoptotic signaling cascades: the first is the death receptor (Fas or tumor necrosis factor receptor)- ... A representative Western blot with a cytochrome c antibody to assay cytochrome c content in the cytosol and mitochondria from ...
  http://circres.ahajournals.org/content/101/11/1113
*  Growth Factor Signaling in Cell Survival: Implications for Cancer Treatment | Journal of Pharmacology and Experimental...
Once in the cytoplasm, cytochrome c binds to apoptotic protease-activating factor-1, which binds and activates caspase-9 ... 1997) Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. ... apoptotic protease-activating factor 1; PKA, cAMP-dependent protein kinase; ANT, adenine nucleotide transporter. ... nerve growth factor), brain-derived neurotrophic factor, and growth factors (insulin-like growth factor-1, platelet-derived ...
  http://jpet.aspetjournals.org/content/298/3/873
*  Rapid extracellular release of cytochrome c is specific for apoptosis and marks cell death in vivo | Blood Journal
Once released, cytochrome c cooperates with apoptotic protease-activating factor-1 and deoxyadenosine triphosphate in caspase-9 ... 5 6 Released cytochrome c then activates the apoptosome-dependent death machinery by binding to Apaf-1, resulting in the ... cytochrome c together with deoxyadenosine triphosphate binds to apoptotic protease-activating factor-1 (Apaf-1), leading to an ... whereas the release of cytochrome c characterizes apoptotic events.. So far, it has only been described that cytochrome c is ...
  http://www.bloodjournal.org/content/98/5/1542?sso-checked=1
*  Apoptosis - Wikipedia
Once cytochrome c is released it binds with Apoptotic protease activating factor - 1 (Apaf-1) and ATP, which then bind to pro- ... Apaf-1 is the protein that turns on caspase 9 by cleavage to begin the caspase cascade that leads to apoptosis. Since a -/- ... Some apoptotic factors are vital during mitochondrial respiration e.g. cytochrome C. Pathological inactivation of apoptosis in ... which are proteases, or enzymes that degrade proteins. The two pathways both activate initiator caspases, which then activate ...
  https://en.wikipedia.org/wiki/Apoptosis
*  UNO (?? ) ??????. UNO-???
... apoptotic protease activating factor-1) with cytochrome c molecules assembled to the tips of each spoke. "Gee, how did it ... Apaf1) and cyt c participate in mitochondria- associated apartment death. Into the bargain, it was accomplishable to complete ... cytochrome c facilitates the self-assembly of the apoptosome, the abhorrent position of extirpation which comprises seven ... while caspases 3 and 9 together with apoptosis protease-activated ingredient 1 ( ...
  http://play-uno.ru/index/comments/?page=1995
*  Edelstar, ?????????
... apoptotic protease activating factor-1) with cytochrome c molecules assembled to the tips of each spoke. The amount of clearing ... In particular, cytochrome c facilitates the self-assembly of the apoptosome, the bad wheel of expiry which comprises seven ... spokes' contain- ing the protein Apaf-1 ( ... ther of these factors participate a post in ovarian cancer 3]. ... and IL-10 whose drop is increased in vitro after phagocytosis of apoptotic bodies (Jack et al. A fourth phase of clinical ...
  http://edelstar-shop.ru/?showpage=1508
*  しっかり練習してきたようです / Chương trình khuyến mại M88Bet / M88 Diễn đàn thành viên
... apoptotic protease activating factor-1) with cytochrome c molecules assembled to the tips of each spoke. Health-giving ... teins such as cytochrome c, apoptosis-inducing factor (AIF), and other mitochondrial pro-apoptotic expiry mediators. Congenital ... ing the protein Apaf-1 ( ... Factors placing the infant or toddler at risk repayment for ... In hypercritical, cytochrome c facilitates the self-assembly of the apoptosome, the bad ring of death which comprises seven ...
  http://ourm88.com/forum/viewtopic.php?pid=829
*  CHINESE HAMSTER APOPTOSIS-RELATED GENES - Patent application
Released cytochrome c binds to the apoptotic protease activating factor, APAF-1, forming a complex that activates procaspase-9 ... CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases. Proc. Natl. Acad. Sci. USA. 93:14486-14491 ... Proteins made in such systems may be designed to include heparin, thrombin, or factor XA protease cleavage sites so that the ... 0347] An Ethidium Bromide/Acridine Orange Assay is used to classify cells in samples collected into apoptotic or non-apoptotic ...
  http://www.patentsencyclopedia.com/app/20110318316
*  Cardiac Hypertrophy Cell Biology - Cell Biology of Disease and Exercise
... apoptotic protease-activating factor) and caspase-9 in the presence of dATP to form an active apoptosome complex. [135][139] ... and pro-apoptotic members of the Bcl-2 protein family. Once released, cytosolic cytochrome c binds to Apaf-1 ( ... TGFβ also signals through TGFβ-activated kinase (TAK1) to activate ATF2 (activated transcription factor 2).[28] Studies of ... Overexpression of tumor necrosis factor- alpha activates both anti- and pro-apoptotic pathways in the myocardium. J Mol Cell ...
  http://pt851.wikidot.com/cardiac-hypertrophy-cell-biology
*  Apaf1 plays a pro-survival role by regulating centrosome morphology and function | Journal of Cell Science
The apoptotic protease activating factor 1 (Apaf1) is one of the most studied proapoptotic proteins. It is the core of the ... anti-apoptotic proteins are downregulated in Apaf1-depleted cells, which, in fact, rapidly release cytochrome c upon apoptotic ... The apoptotic protease activating factor 1 (Apaf1) is the main component of the apoptosome, and a crucial factor in the ... 2000). Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl- ...
  http://jcs.biologists.org/content/124/20/3450
*  Drosophila larvae lacking the bcl-2 gene, buffy, are sensitive to nutrient stress, maintain increased basal target of rapamycin...
... as well as activation of apoptotic protease activating factor 1 (Apaf-1) and caspases to promote death. Apoptosis inducing ... many apoptotic mediators have a dual role. For example, cytochrome c is essential for respiration, a pro-survival function, ... In the simplest current thinking, nutrient deprivation both inactivates Tor and activates AMPK to phosphorylate and activate ... but modulate the apoptotic response to stress [11-13]. Whether release of mitochondrial factors is essential for fruit fly ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3411425/
*  Molecular mechanisms of tumour suppression by the P53-stabilizing compound CP-31398 - UBC Library Open Collections
... apoptotic protease activating factor-1), causing an ATP or dATP-dependent conformational 7 change that allows Apaf-1 to bind ... Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. J. Biol. Chem. 1999, ... PCR PI PIGs PUMA PVDF RB ROS RT SDS SRB TCA TNFR1 TRAIL UV VIN Z-VAD-FMK 5-fluorouracil apoptotic protease activating factor-1 ... 3.2.6 CP-31398 activates caspases-9 and -3 Activation of the mitochondrial apoptotic pathway involves the release of cytochrome ...
  https://open.library.ubc.ca/cIRcle/collections/ubctheses/831/items/1.0090758
*  Apoptotic markers in protozoan parasites | Parasites & Vectors | Full Text
... apoptotic protease activating factor (Apaf-1), which subsequently activates procaspase-9. Even though protozoan parasites and ... In this case, the antibodies used were generated against peptides from T. brucei cytochrome c [44]. No reports of cytochrome c ... although there is some support for the view that cysteine proteases are related to the expression of other apoptotic markers. ... canonical apoptotic marker) and lastly to PS positive/PI positive (late apoptotic marker). Further incubation will render most ...
  https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-3-104
*  History of apoptosis research - Wikipedia
... apoptotic protease activating factor). The team published their results in an article entitled "Apaf-1, a human protein ... participates in cytochrome c-dependent activation of caspase-3". Cell. 90 (3): 405-13. doi:10.1016/S0092-8674(00)80501-2. PMID ... Zou H, Henzel WJ, Liu X, Lutschg A, Wang X (August 1997). "Apaf-1, a human protein homologous to C. elegans CED-4, ... Landmark research by David L. Vaux and colleagues described the anti-apoptotic and tumorigenic (tumor-causing) role of the ...
  https://en.wikipedia.org/wiki/History_of_apoptosis_research