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*  Construction of a chimeric hepatitis C virus replicon based on a strain isolated from a chronic hepatitis C patient |...
Here, we report a new subgenomic replicon based on a strain isolated from a chronically infected pat ... Subgenomic replicons of hepatitis C virus (HCV) have been widely used for studying HCV replication. ... Interaction between the HCV NS3 protein and the host TBK1 protein leads to inhibition of cellular antiviral responses. ... The subgenomic replicon of CCH was replication-deficient in cell culture, due to dysfunctions in NS3 and NS5B. Various JFH1/CCH ...
  https://link.springer.com/article/10.1007/s12250-014-3408-z
*  Development of protease assays and replicons for antiviral studies against FMDV | EUVIRNA
Development of protease assays and replicons for antiviral studies against FMDV. EUVIRNA ER-3 ... Antivirals identified will be developed by Aratana Therapeutics into powerful drugs that offer an alternative for the culling ... Surrogate models will not only be used in the screening and lead optimization process for developing FMDV antivirals, but also ...
  http://euvirna.phrmy.cf.ac.uk/?q=node/20
*  Hepatitis C virus subgenomic replicon requires an active NS3 RNA helicase. - Semantic Scholar
In a hepatoma cell line that has been cured of HCV replicons using interferon, the T450I and W501F replicons synthesized both ... Surprisingly the same phenotype was seen for the E493K replicon. The inability of the E493K replicon to replicate might point ... Con1 subgenomic replicon to ascertain the role of the helicase in viral replication. One new replicon lacked two-thirds of the ... However, the Deltahel, R393A, F438A, and W501A replicons encoded and processed an HCV polyprotein but did not synthesize ...
  https://www.semanticscholar.org/paper/Hepatitis-C-virus-subgenomic-replicon-requires-an-Lam-Frick/2282d500429d4b5a95ae03be767abd40e8c44a14
*  Patent US6153421 - Cloned genomes of infectious hepatitis C viruses and uses thereof - Google Patents
... development of vaccines and diagnostics for HCV and in the development of screening assays for the identification of antiviral ... Efficient hepatitis C virus replicon and its use in identifying antiviral compounds. ... nucleic acid sequence and antiviral agent) would indicate, the degree, if any, of antiviral activity of the candidate antiviral ... may be directly assayed in vivo for antiviral activity by administering the candidate antiviral agent to a chimpanzee ...
  http://www.google.com/patents/US6153421?dq=5179747
*  Viruses | Free Full-Text | Hepatitis C Virus NS3/4A Protease Inhibitors: A Light at the End of the Tunnel
... review summarizes the NS3 protease inhibitors currently challenged in clinical trials as one of the most promising antiviral ... that is essential for viral polyprotein maturation and contributes to the evasion of the host innate antiviral immunity by HCV ... HCV replicon; clinical trial HCV; protease inhibitor; NS3 protease; antiviral therapy; HCV replicon; clinical trial ... Antiviral Strategies for Pandemic and Seasonal Influenza. Next Article in Special Issue. Last Stop Before Exit - Hepatitis C ...
  http://www.mdpi.com/1999-4915/2/8/1752
*  Resistance Analysis and Characterization of a Thiazole Analogue, BP008, as a Potent Hepatitis C Virus NS5A Inhibitor |...
VX-950, a novel hepatitis C virus (HCV) NS3-4A protease inhibitor, exhibits potent antiviral activities in HCV replicon cells. ... Isolation of resistant replicons.The selection of resistant replicon cells was performed by growing HCV genotype 1b replicon ... The HCV replicon cell lines were isolated from colonies as described by Lohman et al. (37). The culture medium of replicon ... Huh7.5-HCV1b replicon (a HCV genotype 1b [con1-based] subgenomic replicon clone with cell culture-adaptive mutation S2204I in ...
  https://aac.asm.org/content/56/1/44
*  Research Projects | EUVIRNA
Development of protease assays and replicons for antiviral studies against FMDV Finished. ... Antivirals against polio and other enteroviruses Finished. ESR-12. Rega Institute for Medical Research, KU Leuven. Belgium. ... Dissecting RdRp mechanisms; antivirals as tools for structural and functional studies Finished. ... Understanding the molecular mechanisms of virus replication is crucial for antiviral drug development. In EUVIRNA, virologists ...
  http://euvirna.phrmy.cf.ac.uk/?q=node/2
*  Joseph (Jody) Puglisi | Stanford Medicine Profiles
... recently was shown to have unexpected antiviral activity in the HCV replicon system and in chronically infected patients. A ... a key kinase that regulates the cell's innate antiviral response. These observations could explain the clinical antiviral ... Translational insensitivity to potent activation of PKR by HCV IRES RNA ANTIVIRAL RESEARCH Shimoike, T., Mckenna, S. A., ... The RNA-dependent protein kinase (PKR) plays an integral role in the antiviral response to cellular infection. PKR contains ...
  https://med.stanford.edu/profiles/joseph-puglisi
*  Protocols and Video Articles Authored by Silke Stertz
They further imply that the observed insensitivity of the 1918 virus-based replicon to the antiviral activity of human MxA is a ... S20 shows potent antiviral activity (IC50 = 80 nM) and low toxicity (CC50 = 40 μM), yielding a selectivity index of 500 and ... Interferon-induced, Antiviral Human MxA Protein Localizes to a Distinct Subcompartment of the Smooth Endoplasmic Reticulum ... MxA is induced by interferons-alpha/beta (IFN-alpha/beta) and is a key component of the antiviral response against RNA viruses ...
  https://www.jove.com/author/Silke_Stertz
*  Mavyret | FULL Prescribing Information | PDR.net
Antiviral Activity. In HCV replicon assays, glecaprevir had median EC50 values of 0.08-4.6 nM against laboratory and clinical ... Y93H in genotype 1a replicons, F28S + M31I or P29S + K30G in genotype 2a replicons, and Y93H in genotype 3a replicons. The ... Evaluation of combination of glecaprevir and pibrentasvir showed no antagonism in antiviral activity in HCV genotype 1 replicon ... HIV-Antiviral Agents:. Atazanavir. ↑ glecaprevir. ↑ pibrentasvir. Coadministration is contraindicated due to increased risk of ...
  http://www.pdr.net/full-prescribing-information?druglabelid=24125
*  The enterovirus protease inhibitor rupintrivir exerts cross-genotypic anti-norovirus activity and clears cells from the...
Antiviral activity of rupintrivir against the Norwalk virus replicon, represented by relative genome quantification (Norwalk ... Antiviral effect of the combination of either rupintrivir and 2CMC (A) or rupintrivir and T-705 (B). Values in the zero plane ... The design of antiviral molecules targeting the norovirus protease could be a valuable approach for the treatment and/or ... Antiviral activity of rupintrivir against MNV as quantified by (i) virus-induced cytopathic effect (CPE) reduction assay using ...
  https://www.ncbi.nlm.nih.gov/pubmed/24890597
*  Sofosbuvir and Velpatasvir for HCV Genotype 1, 2, 4, 5, and 6 Infection
... is a new pangenotypic HCV NS5A inhibitor with antiviral activity against HCV replicons in genotypes 1 through 6.9-11 In phase 2 ... There are now effective combinations of direct-acting antiviral agents for most patients, but in choosing an appropriate ... patterns of antiviral resistance. Some regimens also include ribavirin, which has known hematologic and other side effects and ... of velpatasvir in the HCV genotype 1a replicon. Arginine (R) variants at position 30 of the NS5A protein were present at ...
  http://natap.org/2015/AASLD/AASLD_66.htm
*  Books by Subject - Lane Medical Library - Stanford University School of Medicine
Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds / Sofia L. Alcaraz- ... Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models / Joke Van den ... Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease ... Cell-based antiviral assays for screening and profiling inhibitors against dengue virus / Edwin Yunhao Gong ... [et al.] -- ...
  http://lane.stanford.edu/biomed-resources/ebsubjectbrowse.html?m=Pharmacology
*  ADAR - Wikipedia
Taylor DR, Puig M, Darnell ME, Mihalik K, Feinstone SM (2005). "New antiviral pathway that mediates hepatitis C virus replicon ... This seems to be true for the HCV replicon, Lymphocytic choriomeningitis LCMV, and polyomavirus ADAR1 is known to be proviral ... Samuel CE (March 2011). "Adenosine deaminases acting on RNA (ADARs) are both antiviral and proviral". Virology. 411 (2): 180-93 ... viruses are thought to use ADAR1 as a positive replication factor by selectively suppressing dsRNA-dependent and antiviral ...
  https://en.wikipedia.org/wiki/ADAR
*  Dengue and Zika: Control and Antiviral Treatment Strategies | SpringerLink
Establishment and Application of Flavivirus Replicons Beate M. Kümmerer. Pages 165-173 ... Viral Entry and NS1 as Potential Antiviral Drug Targets Aravinda M. de Silva, Félix A. Rey, Paul R. Young, Rolf Hilgenfeld, ... Structural Insights into the Broad-Spectrum Antiviral Target Endoplasmic Reticulum Alpha-Glucosidase II ... Nucleocytoplasmic Trafficking of Dengue Non-structural Protein 5 as a Target for Antivirals ...
  https://link.springer.com/book/10.1007%2F978-981-10-8727-1
*  Zakład Biofizyki | Instytut Biochemii i Biofizyki
... its mechanism of action and antiviral activity in the replicon system. Antimicrobial Agents and Chemotherapy (2008) 52: 393-401 ...
  https://www.ibb.waw.pl/pl/struktura/zaklady-i-pracownie-ibb/zaklad-biofizyki
*  Synergistic inhibition of human cytomegalovirus replication by interferon-alpha/beta and interferon-gamma | Virology Journal |...
Synergistic antiviral activity of human interferon combinations in the hepatitis C virus replicon system. J Interferon Cytokine ... suggesting action via distinct antiviral pathways.. Table 2 Degree of antiviral interaction between IFN-α/β and IFN-γ ... The antiviral activity of IFNs on HCMV plaque formation was further assessed by generating dose-response curves (Figure 2A). ... The lack of GFP-positive cells in IFN-α/β and IFN-γ-treated groups suggested to us that the synergistic antiviral activities ...
  https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-2-14
*  WikiGenes - Replicon
The SKI2 antiviral gene represses the copy numbers of the L-A and L-BC viruses and the 20S RNA replicon, apparently by ... Psychiatry related information on Replicon. *The high permissiveness of the cured cells for the replicon was abolished by ... Analytical, diagnostic and therapeutic context of Replicon. *Using a selectable subgenomic HCV replicon cell culture system, we ... Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral ...
  https://www.wikigenes.org/e/mesh/e/399.html
*  Antiviral Activity of Bictegravir (GS-9883), a Novel Potent HIV-1 Integrase Strand Transfer Inhibitor with an Improved...
Stacey Eng and Christian Voitenleitner for antiviral testing in HCV replicon cells, Ruoyu Gong for antiviral testing against ... Antiviral Agents. Antiviral Activity of Bictegravir (GS-9883), a Novel Potent HIV-1 Integrase Strand Transfer Inhibitor with an ... BIC is synergistic in combination with approved HIV-1 antiviral drugs.To assess the antiviral activity of BIC in combination ... For the antiviral assay, CD4+ T lymphocytes at a density of 4 × 106 cells per ml were infected in bulk culture with HIV-1 BaL ...
  https://aac.asm.org/content/60/12/7086
*  Future Antivirals: Latest Developments in HIV, Hepatitis, Herpes, Influenza, RSV & SARS : Pharmaceuticals : All
GI-GPx is down-regulated in replicon cells *Overexpression of GI-GPx suppresses RNA replication *Identification of compounds ... 10:20 ANTIVIRAL THERAPIES, ANTIVIRAL STRATEGIES Sabine Hallenberger, Director, Virology Research, Bayer *Similarities, ... Current antiviral therapies *Medivirs' approach - novel nucleoside analogues *HIV resistance *The outlook for the antiviral ... SMi's inaugural conference Antivirals, aims to cover the latest approaches in antiviral research, drug discovery and ...
  http://www.smi-online.co.uk/pharmaceuticals/archive/10-2005/workshop/?o=login&dl=br&p1=2254
*  Protocols and Video Articles Authored by Brian J. Geiss
... displays antiviral effects in dengue virus replicon assays and inhibits growth of West Nile and yellow fever viruses with low ... was found to possess significant antiviral activity in a dengue virus subgenomic replicon assay. Further testing of BG-323 ... In this study we further characterized BG-323's antiviral activity in vitro and in vivo. We found that BG-323 was able to ... The most intriguing ML283 analogue inhibited DENV NS3 with an IC50 value of 500 nM and was active against the DENV replicon. ...
  https://www.jove.com/author/Brian++J._Geiss
*  2018 July | mirna inhibitor
Supernatants from HCV-infected PHH cultures mediated antiviral activity upon transfer to HCV-replicon-containing cells. This ... To identify the cytokines and cells that drive ISG induction 上海皓元医药股份有限公司 and mediate antiviral activity during acute HCV ...
  http://mirnainhibitor.com/2018/07
*  CiNii 論文 - Advances in Genomic Research on Hepatitis C Virus with a Useful Tool, Replicon...
An NS 3 protease inhibitor with antiviral effects in humans infected with hepatitis C virus LAMARRE D ... Advances in Genomic Research on Hepatitis C Virus with a Useful Tool, Replicon System * * NAKAMURA Mitsuyasu ... Subgenomic replicon derived from a cell line infected with the hepatitis C virus KISHINE H ... Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons GOSERT R ...
  http://ci.nii.ac.jp/naid/10021988439
*  Karla Kirkegaard | Stanford Medicine Profiles
When superinfecting poliovirus resistant to the antiviral compound guanidine was used, the RNA replication of the replicon RNAs ... For example, the export of most proinflammatory and antiviral cytokines, which recruit lymphocytes and initiate antiviral ... RBV resistance in HCV replicon cell lines can be conferred by changes in the cell line or by mutations in the HCV replicon. ... RBV-resistant, HCV replicon-containing cell lines were generated, and the majority of RBV resistance was found to be conferred ...
  https://med.stanford.edu/profiles/karla-kirkegaard
*  Peter Sarnow | Stanford Medicine Profiles
When superinfecting poliovirus resistant to the antiviral compound guanidine was used, the RNA replication of the replicon RNAs ... RNAi, Antiviral after all. Science Sagan, S. M., Sarnow, P. 2013; 342 (6155): 207-208 View details for DOI 10.1126/science. ... The Imd Pathway Is Involved in Antiviral Immune Responses in Drosophila PLOS ONE Costa, A., Jan, E., Sarnow, P., Schneider, D. ... Replication of HCV replicon NNeo/C-5B RNA (genotype 1b) is significantly suppressed in HuH-7-derived cells expressing green ...
  https://med.stanford.edu/profiles/peter-sarnow