*  Cryptosporidiosis - Wikipedia
Detecting antigens is yet another way to diagnose the disease. This can be done with direct fluorescent antibody (DFA) ... Another form of microscopy is fluorescent microscopy done by staining with auramine. Other staining techniques include acid- ... Antibodies in the serum of humans and animals infected with Cryptosporidium parvum react with several antigens, one of which is ... vaccine has produced an antibody response in a large group of cows and also antibody response in calves fed rCP15/60-immune ...
  https://en.wikipedia.org/wiki/Cryptosporidiosis
*  August 7, 2002 - Payment Determinations for Calendar Year 2003 for New Clinical Laboratory Tests
... infectious agent antigen detection by immunofluorescent technique rather than direct fluorescent antibody).. Proposed code ... direct fluorescent antibody; 8727X Cytomegalovirus, direct fluorescent antibody; and 8905X Leukocyte count, fecal.Summarized ... Proposed code 8726X: Enterovirus, direct fluorescent antibody. Nature of the test method:. This code in the series 87260-87299 ... Clinical applications for a primary source enterovirus direct fluorescent antibody test are not well established. The current ...
  https://www.asm.org/index.php/publicpolicy-2/statements-testimony?id=2370
*  MLN1202 in Treating Patients With Bone Metastases
Monoclonal antibodies, such MLN1202, can block tumor growth in different ways. Some block the ability of tumor c... ... Fluorescent Antibody Technique. Test for tissue antigen using either a direct method, by conjugation of antibody with ... Fluorescent Antibody Technique, Direct. A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an ... Fluorescent Antibody Technique, Indirect. A form of fluorescent antibody technique commonly used to detect serum antibodies and ...
  https://www.bioportfolio.com/resources/trial/70723/MLN1202-in-Treating-Patients-With-Bone-Metastases.html
*  Giardiasis | Pediatric Opportunistic Infection | AIDSinfo
... direct fluorescent antibody (DFA) assays; by detecting soluble stool antigens using enzyme immunoassays (EIA); or, by using ... molecular techniques including polymerase chain reaction.21,22 Identification of both trophozoites and cysts can be made on ... Use of polyclonal antisera or monoclonal antibodies against Giardia-specific antigens has improved diagnostic testing. Studies ... All fluorescent antibody tests had 100% sensitivity and specificity.24 These rapid diagnostic tests can be positive before and ...
  https://aidsinfo.nih.gov/guidelines/html/5/pediatric-opportunistic-infection/402/giardiasis
*  Laboratory Tests for Infectious Diseases
Direct Fluorescent Antibody (DFA) is the straightforward detection of antigens using fluorescently labeled antigen-specific ... Direct ELISA is a technique for measuring antigen using competition for antibody binding sites between enzyme-labeled antigen ... Antigen, if present, is then "sandwiched" between bound antibodies and antigen-specific, enzyme-labeled antibodies. Antigen- ... analytical technique for quantitation of antigen or antibody in which enzyme-labeled antibody or antigen is bound to a solid ...
  https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/TestsForInfectDiseases.htm
*  Patent US4257774 - Intercalation inhibition assay for compounds that interact with DNA or RNA - Google Patents
Measurement of binding inhibition utilizes the rapid, repeatable technique of fluorescence polarization. ... This technique has been applied to measuring the interactions of antigens and antibodies, enzymes and substrates, membranes and ... Fluorescence polarization studies of the direct binding of several fluorescent intercalators to DNA has been reported ( ... The technique of fluorescence polarization is well-documented in the publications of Weber; "Fluorescence Techniques in Cell ...
  http://www.google.com.au/patents/US4257774
*  Testing For Chlamydia ASAP
An alternative technique useful for Chlamydia testing is named the direct fluorescent antibody stain. It functions by seeking ... the Chlamydia antigens, elements that activate the discharge of antibodies that will counteract and exterminate them. ... Testing techniques. The first example of a Chlamydia testing method is known as the Nucleic Acid Amplification Tests or NAAT. ...
  https://www.stdrapidtestkits.com/blog/testing-for-chlamydia-asap
*  Plus it
The lungs of three infants, two with bronchiolitis and one with pneumonia, were examined by fluorescent antibody techniques for ... virus antigen, whereas in R.S. virus pneumonia the mucosal necrosis and alveolar and interstitial inflammation are the result ... of direct virus damage to the lungs. The alternative explanation put forward is that the process may be a type 3 allergic ...
  http://www.bmj.com/content/1/5692/327
*  Reasons To Get A Chlamydia Test Promptly
An alternative technique useful for Chlamydia testing is known as the direct fluorescent antibody stain. It does the job by ... searching for the Chlamydia antigens, elements that result in the launch of antibodies that will counteract and slaughter them ...
  https://www.stdrapidtestkits.com/blog/reasons-to-get-a-chlamydia-test-promptly
*  New technique efficiently turns antibodies into highly tuned 'nanobodies' - Healthcanal.com : Healthcanal.com
... directed against two fluorescent proteins that biologists often use as markers to visualize activity within cells: GFP and ... keeping only the antigen-binding section to create nanobodies. ... New technique efficiently turns antibodies into highly tuned ' ... Home Medical Breakthroughs New technique efficiently turns antibodies into highly tuned 'nanobodies' ... started by making antibody sequence databases from RNA isolated out of antibody-producing cells in the llamas' bone marrow. ...
  https://www.healthcanal.com/medical-breakthroughs/56934-new-technique-efficiently-turns-antibodies-into-highly-tuned-nanobodies.html
*  PPT - Chapter 18 PowerPoint Presentation - ID:687591
Precipitation: Soluble antigens *Fluorescent-antibody technique: Antibodies linked to fluorescent dye *Complement fixation: ... Enzyme-Linked Immunosorbent Assay Viral Diseases in Humans(Direct ELISA) Figure 18.12a ... Antibody Titer Viral Diseases in Humans *Is the concentration of antibodies against a particular antigen ... Viral hemagglutination inhibition tests for antibodies by the antibodies' ability to prevent viruses from agglutinating RBCs. ...
  https://www.slideserve.com/adonica/practical-applications-of-immunology
*  Immunofluorescence - Wikipedia
... an antigen can also be conjugated to the antibody with a fluorescent probe in a technique called fluorescent antigen technique ... Since the number of fluorescent molecules that can be bound to the primary antibody is limited, direct immunofluorescence is ... This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets ... There are two classes of immunofluorescence techniques, primary (or direct) and secondary (or indirect). Primary (direct) ...
  https://en.wikipedia.org/wiki/Immunofluorescence
*  Infectious disease
fluorescent (fluorescently labeled antibodies) can be directed to bind to and identify a specific antigens present on a ... staining techniques, and can be made exquisitely specific when used in combination with antibody based techniques. For example ... These tests are based upon the ability of an antibody to bind specifically to an antigen. The antigen, usually a protein or ... a direct effect upon a pathogen, such as antibody-initiated complement-dependent bacteriolysis, opsonoization, phagocytosis and ...
  http://www.cs.mcgill.ca/~rwest/link-suggestion/wpcd_2008-09_augmented/wp/i/Infectious_disease.htm
*  Syphilis Infection during pregnancy: fetal risks and clinical management.
Other possible methods include direct fluorescent antibody (DFA) testing and the rabbit infectivity test (not used in clinical ... Dark field microscopy is the most specific technique for diagnosing syphilis when an active chancre or condyloma latum is ... TTs detect an interaction between serum immunoglobulins and surface antigens of Treponema pallidum. They include the ... Secondary syphilis is always characterized by a reactive VDRL, with a titer greater than 1/16 [2]. The titer of antibodies ...
  http://www.biomedsearch.com/nih/Syphilis-Infection-during-Pregnancy-Fetal/22829747.html
*  Rabies Diagnosis
Direct Fluorescent Antibody Test. Direct fluorescent antibody (DFA) test, which detects antigens of the rabies virus in the ... Other Diagnostic Techniques. Direct rapid immunochemistry tests are also accepted to have adequate sensitivity and specificity ... This is done with the use of fluorescently marked antibodies that are applied to the tissue and observing the fluorescent ... Deciphering Antibodies with Next-Generation Protein Sequencing Technology. Mingjie Xie. Mr. Mingjie Xie, MSc, MBA, is the co- ...
  https://www.news-medical.net/health/Rabies-Diagnosis.aspx
*  Immunohistochemistry - EDRN Public Portal
The primary antibody is conjugated to fluorescein binds directly with the antigen and can be detected by the fluorescent tag ... Direct immunofluorescence method is used to detect the deposit of immunoglobulins, complement components, fibrinogen, etc. in ... This technique is usually performed on frozen sections. ... using a fluorescent microscope. Immunoperoxidase Staining Pre- ...
  https://edrn.nci.nih.gov/resources/standard-operating-procedures/assays/immunohistochemistry
*  GBMC Test Dictionary
Direct ELISA is a technique for measuring antigen using competition for antibody binding sites between enzyme- labeled antigen ... Direct fluorescent antibody (DFA) is the straightforward detection of antigens using fluorescently labeled antigen-specific ... Antigen, if present, is then 'sandwiched' between bound antibodies and antigen-specific, enzyme- labeled antibodies. Antigen- ... Particulate Antigen-Antibody Reaction Assays Direct agglutination (DA) is a general term for techniques which use the ...
  http://www.specialtylabs.com/clients/gbmc/methodology.asp?id=WBC
*  indirect fluorescent antibody technique
The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti- ... Using direct enzyme linked immunosorbent assay for the detection of IgG antibody on Bartonella henselae among healthy people in ... indirect fluorescent antibody technique. Summary. Summary: A form of fluorescent antibody technique commonly used to detect ... viral antibodies*biological markers*fluorescent antibody technique*antineutrophil cytoplasmic antibodies*monoclonal antibodies* ...
  https://www.labome.org/topics/diagnostic/diagnosis/laboratory/immunologic/fluorescent/indirect-fluorescent-antibody-technique-16534.html
*  Rabies - Wikipedia
6: The fluorescent antibody test". In Kaplan, M.M.; Koprowski, H. Laboratory techniques in rabies. Monograph series. 23 (3rd ed ... Microscopic analysis of samples is the only direct method that allows for the identification of rabies virus-specific antigen ... to bind to and allow the visualisation of rabies antigen using fluorescent microscopy techniques. ... The reference method for diagnosing rabies is the fluorescent antibody test (FAT), an immunohistochemistry procedure, which is ...
  https://en.wikipedia.org/wiki/Rabies
*  Infection - Wikipedia
For example, the use of antibodies made artificially fluorescent (fluorescently labeled antibodies) can be directed to bind to ... These tests are based upon the ability of an antibody to bind specifically to an antigen. The antigen, usually a protein or ... Complex serological techniques have been developed into what are known as Immunoassays. Immunoassays can use the basic antibody ... Immunity mediated by these two factors may be manifested by: a direct effect upon a pathogen, such as antibody-initiated ...
  https://en.wikipedia.org/wiki/Infection
*  Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a Polyaniline/Graphene Nanocomposite Functionalized Impedimetric...
It is directed at both academic researchers and practicing engineers. Journal of Nanomaterials will highlight the continued ... while flow cytometry also has to be done with fluorescent labeled antibodies. Moreover this advanced cell analysis technique ... To evaluate the interaction between CSPG4 antibody and antigen, the D2.8.5 antibody functionalized PANI@GO/ITO electrodes were ... In addition, the direct and sandwich ELISA were conducted to analyze the same cell membrane protein lysate. The results support ...
  https://www.hindawi.com/journals/jnm/2016/7834657/
*  Neuronal lineage marker - Wikipedia
Immunohistochemistry is a technique that uses antibodies with fluorescent staining tags that target a specific antigen present ... Cellular fates are analysed by staining with antibodies directed against antigens specific for astrocytes, oligodendrocytes, ... He created an immunoflorescent technique for labelling the antibodies. This technique continues to be widely used in ... Coons used for the first time a revolutionary technique that uses the principle of antibodies binding specifically to antigens ...
  https://en.wikipedia.org/wiki/Neuronal_lineage_marker
*  Laboratory Diagnosis of Human Rabies: Recent Advances
Demonstration of Viral Antigen. 3.2.1. Fluorescent Antibody Technique (FAT). The most widely used test for postmortem rabies ... recommends further development of direct rapid immunohistochemistry tests as an alternative to the direct fluorescent antibody ... D. J. Dean, M. K. Ableseth, and P. Atanasiu, "The fluorescent antibody test," in Laboratory Techniques in Rabies, F.-X. Meslin ... R. A. Goldwasser and R. E. Kissling, "Fluorescent antibody staining of street and fixed rabies virus antigens," Proceedings of ...
  https://www.hindawi.com/journals/tswj/2013/569712/
*  US Patent # 6,326,472. Human receptor proteins; related reagents and methods - Patents.com
Antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositi ... Thus, in vitro assays of the present invention will often use antibodies or antigen binding segments of these antibodies, or ... Also, while many of the techniques described are directed to the IL-1RD9 reagents, corresponding methods will typically be ... substrate-labeled fluorescent immunoassay (SLFIA) and the like. For example, unlabeled antibodies can be employed by using a ...
  http://patents.com/us-6326472.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The antigen-antibody reactions are observed microscopically. The protocol has three main steps: 1) preparation of a bacterial ... Antibodies, Monoclonal, Green Fluorescent Proteins, Plant Proteins, Recombinant Proteins, Vaccines, Synthetic, Virus-Like ... The technique may be applied to numerous human specific pathogens that infect the blood stream. ... Optimization of Agrobacterium cultivation in AB medium allows direct dilution of the bacterial culture in Milli-Q water, ...
  https://www.jove.com/visualize/abstract/23805230/mutant-native-outer-membrane-vesicles-combined-with-serogroup